Hoppert, Michael

[["dc.bibliographiccitation.artnumber","624"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schilling, Tobias"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Hertel, Robert"],["dc.date.accessioned","2019-07-09T11:49:42Z"],["dc.date.available","2019-07-09T11:49:42Z"],["dc.date.issued","2018"],["dc.description.abstract","We present the recently isolated virus vB_BthP-Goe4 infecting Bacillus thuringiensis HD1. Morphological investigation via transmission electron microscopy revealed key characteristics of the genus Phi29virus, but with an elongated head resulting in larger virion particles of approximately 50 nm width and 120 nm height. Genome sequencing and analysis resulted in a linear phage chromosome of approximately 26 kb, harbouring 40 protein-encoding genes and a packaging RNA. Sequence comparison confirmed the relation to the Phi29virus genus and genomes of other related strains. A global average nucleotide identity analysis of all identified φ29-like viruses revealed the formation of several new groups previously not observed. The largest group includes Goe4 and may significantly expand the genus Phi29virus (Salasvirus) or the Picovirinae subfamily."],["dc.description.sponsorship","Volkswagen Foundation"],["dc.identifier.doi","10.3390/v10110624"],["dc.identifier.pmid","30428528"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15746"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59610"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1999-4915"],["dc.relation.issn","1999-4915"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Genomic Analysis of the Recent Viral Isolate vB_BthP-Goe4 Reveals Increased Diversity of φ29-Like Phages."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Willms, Inka M."],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Hertel, Robert"],["dc.date.accessioned","2019-07-09T11:43:27Z"],["dc.date.available","2019-07-09T11:43:27Z"],["dc.date.issued","2017-06-12"],["dc.description.abstract","The Spounavirinae viruses are ubiquitous in nature and have an obligatory virulent lifestyle. They infect Firmicutes, a bacterial phylum containing an array of environmental non-pathogenic and pathogenic organisms. To expand the knowledge of this viral subfamily, new strains were isolated and investigated in this study. Here we present two new viruses, vB_BsuM-Goe2 and vB_BsuM-Goe3, isolated from raw sewage and infecting Bacillus species. Both were morphologically classified via transmission electron microscopy (TEM) as members of the Spounavirinae subfamily belonging to the Myoviridae family. Genomic sequencing and analyses allowed further affiliation of vB_BsuM-Goe2 to the SPO1-like virus group and vB_BsuM-Goe3 to the Bastille-like virus group. Experimentally determined adsorption constant, latency period, burst size and host range for both viruses revealed different survival strategies. Thus vB_BsuM-Goe2 seemed to rely on fewer host species compared to vB_BsuM-Goe3, but efficiently recruits those. Stability tests pointed out that both viruses are best preserved in LB-medium or TMK-buffer at 4 or 21 °C, whereas cryopreservation strongly reduced viability."],["dc.identifier.doi","10.3390/v9060146"],["dc.identifier.pmid","28604650"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14536"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58890"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1999-4915"],["dc.relation.issn","1999-4915"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Characterization of Bacillus Subtilis Viruses vB_BsuM-Goe2 and vB_BsuM-Goe3"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","241"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schilling, Tobias"],["dc.contributor.author","Dietrich, Sascha"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Hertel, Robert"],["dc.date.accessioned","2019-07-09T11:45:29Z"],["dc.date.available","2019-07-09T11:45:29Z"],["dc.date.issued","2018"],["dc.description.abstract","Phages are currently under discussion as a solution for the antibiotic crisis, as they may cure diseases caused by multi-drug-resistant pathogens. However, knowledge of phage biology and genetics is limited, which impedes risk assessment of therapeutic applications. In order to enable advances in phage genetic research, the aim of this work was to create a toolkit for simple and fast genetic engineering of phages recruiting Bacillus subtilis as host system. The model organism B. subtilis represents a non-pathogenic surrogate of its harmful relatives, such as Bacillus anthracis or Bacillus cereus. This toolkit comprises the application CutSPR, a bioinformatic tool for rapid primer design, and facilitates the cloning of specific CRISPR-Cas9-based mutagenesis plasmids. The employment of the prophage-free and super-competent B. subtilis TS01 strain enables an easy and fast introduction of specific constructs for in vivo phage mutagenesis. Clean gene deletions and a functional clean gene insertion into the genome of the model phage vB_BsuP-Goe1 served as proof of concept and demonstrate reliability and high efficiency. The here presented toolkit allows comprehensive investigation of the diverse phage genetic pool, a better understanding of phage biology, and safe phage applications."],["dc.identifier.doi","10.3390/v10050241"],["dc.identifier.pmid","29734705"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15225"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59239"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1999-4915"],["dc.relation.issn","1999-4915"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","A CRISPR-Cas9-Based Toolkit for Fast and Precise In Vivo Genetic Engineering of Bacillus subtilis Phages"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e00819-20"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","40"],["dc.bibliographiccitation.journal","Microbiology Resource Announcements"],["dc.bibliographiccitation.lastpage","3"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Winkler, Lucia"],["dc.contributor.author","Münker, Marc F."],["dc.contributor.author","Brunotte, Susanne"],["dc.contributor.author","Rohlmann, Lina"],["dc.contributor.author","Diez Alfageme, Alvaro"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Reitner, Joachim"],["dc.contributor.author","Nacke, Heiko"],["dc.date.accessioned","2020-10-08T11:07:42Z"],["dc.date.accessioned","2021-10-27T13:14:04Z"],["dc.date.available","2020-10-08T11:07:42Z"],["dc.date.available","2021-10-27T13:14:04Z"],["dc.date.issued","2020"],["dc.description.abstract","We sequenced the metagenome of an anoxygenic photosynthetic consortium originating from pond water and reconstructed four metagenome-assembled genomes. These genomes include Desulfocapsa, Paludibacter, Lamprocystis, and Rhodocyclaceae representatives and indicate the presence of genes for dissimilatory sulfate reduction and oxidation of reduced sulfur compounds."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.1128/MRA.00819-20"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17589"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91829"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","2576-098X"],["dc.relation.orgunit","Fakultät für Geowissenschaften und Geographie"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Metagenome-Assembled Genome Sequences from an Anoxygenic Photosynthetic Consortium Involved in Sulfur Cycling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1607"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Biogeosciences"],["dc.bibliographiccitation.lastpage","1627"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Rincon-Tomas, Blanca"],["dc.contributor.author","Duda, Jan-Peter"],["dc.contributor.author","Somoza, Luis"],["dc.contributor.author","González, Francisco Javier"],["dc.contributor.author","Schneider, Dominik"],["dc.contributor.author","Medialdea, Teresa"],["dc.contributor.author","Santofimia, Esther"],["dc.contributor.author","López-Pamo, Enrique"],["dc.contributor.author","Madureira, Pedro"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Reitner, Joachim"],["dc.date.accessioned","2019-07-09T11:51:57Z"],["dc.date.available","2019-07-09T11:51:57Z"],["dc.date.issued","2019"],["dc.description.abstract","Azooxanthellate cold-water corals (CWCs) have a global distribution and have commonly been found in areas of active fluid seepage. The relationship between the CWCs and these fluids, however, is not well understood. This study aims to unravel the relationship between CWC development and hydrocarbon-rich seepage in Pompeia Province (Gulf of Cádiz, Atlantic Ocean). This region is comprised of mud volcanoes (MVs), coral ridges and fields of coral mounds, which are all affected by the tectonically driven seepage of hydrocarbon-rich fluids. These types of seepage, for example, focused, scattered, diffused or eruptive, is tightly controlled by a complex system of faults and diapirs. Early diagenetic carbonates from the currently active Al Gacel MV exhibit δ13C signatures down to −28.77 ‰ Vienna Pee Dee Belemnite (VPDB), which indicate biologically derived methane as the main carbon source. The same samples contain 13C-depleted lipid biomarkers diagnostic for archaea such as crocetane (δ13C down to −101.2 ‰ VPDB) and pentamethylicosane (PMI) (δ13C down to −102.9 ‰ VPDB), which is evidence of microbially mediated anaerobic oxidation of methane (AOM). This is further supported by next generation DNA sequencing data, demonstrating the presence of AOM-related microorganisms (ANMEs, archaea, sulfate-reducing bacteria) in the carbonate. Embedded corals in some of the carbonates and CWC fragments exhibit less negative δ13C values (−8.08 ‰ to −1.39 ‰ VPDB), pointing against the use of methane as the carbon source. Likewise, the absence of DNA from methane- and sulfide-oxidizing microbes in sampled coral does not support the idea of these organisms having a chemosynthetic lifestyle. In light of these findings, it appears that the CWCs benefit rather indirectly from hydrocarbon-rich seepage by using methane-derived authigenic carbonates as a substratum for colonization. At the same time, chemosynthetic organisms at active sites prevent coral dissolution and necrosis by feeding on the seeping fluids (i.e., methane, sulfate, hydrogen sulfide), allowing cold-water corals to colonize carbonates currently affected by hydrocarbon-rich seepage."],["dc.identifier.doi","10.5194/bg-16-1607-2019"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16248"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60051"],["dc.language.iso","en"],["dc.subject.ddc","570"],["dc.title","Cold-water corals and hydrocarbon-rich seepage in Pompeia Province (Gulf of Cádiz) – living on the edge"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]