Fernandes dos Reis, Micael

[["dc.bibliographiccitation.artnumber","e25520"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Reis, Micael"],["dc.contributor.author","Vieira, Cristina P."],["dc.contributor.author","Morales-Hojas, Ramiro"],["dc.contributor.author","Aguiar, Bruno"],["dc.contributor.author","Rocha, Hélder"],["dc.contributor.author","Schlötterer, Christian"],["dc.contributor.author","Vieira, Jorge"],["dc.date.accessioned","2020-06-29T06:54:33Z"],["dc.date.available","2020-06-29T06:54:33Z"],["dc.date.issued","2011"],["dc.description.abstract","The molecular basis of short term cold resistance (indexed as chill-coma recovery time) has been mostly addressed in D. melanogaster, where candidate genes (Dca (also known as smp-30) and Frost (Fst)) have been identified. Nevertheless, in Drosophila, the ability to tolerate short term exposure to low temperatures evolved several times independently. Therefore, it is unclear whether variation in the same candidate genes is also responsible for short term cold resistance in distantly related Drosophila species. It should be noted that Dca is a candidate gene for cold resistance in the Sophophora subgenus only, since there is no orthologous gene copy in the Drosophila subgenus. Here we show that, in D. americana (Drosophila subgenus), there is a north-south gradient for a variant at the 5' non-coding region of regucalcin (a Dca-like gene; in D. melanogaster the proteins encoded by the two genes share 71.9% amino acid identities) but in our D. americana F2 association experiment there is no association between this polymorphism and chill-coma recovery times. Moreover, we found no convincing evidence that this gene is up-regulated after cold shock in both D. americana and D. melanogaster. Size variation in the Fst PEST domain (putatively involved in rapid protein degradation) is observed when comparing distantly related Drosophila species, and is associated with short term cold resistance differences in D. americana. Nevertheless, this effect is likely through body size variation. Moreover, we show that, even at two hours after cold shock, when up-regulation of this gene is maximal in D. melanogaster (about 48 fold expression change), in D. americana this gene is only moderately up-regulated (about 3 fold expression change). Our work thus shows that there are important differences regarding the molecular basis of cold resistance in distantly related Drosophila species."],["dc.identifier.doi","10.1371/journal.pone.0025520"],["dc.identifier.pmid","21991316"],["dc.identifier.scopus","2-s2.0-80053451103"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66757"],["dc.identifier.url","http://www.scopus.com/inward/record.url?eid=2-s2.0-80053451103&partnerID=MN8TOARS"],["dc.language.iso","en"],["dc.relation.eissn","1932-6203"],["dc.relation.issn","1932-6203"],["dc.title","A comparative study of the short term cold resistance response in distantly related Drosophila species: The role of regucalcin and Frost"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3152"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Genome Biology and Evolution"],["dc.bibliographiccitation.lastpage","3166"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Reis, Micael"],["dc.contributor.author","Vieira, Cristina P."],["dc.contributor.author","Lata, Rodrigo"],["dc.contributor.author","Posnien, Nico"],["dc.contributor.author","Vieira, Jorge"],["dc.date.accessioned","2019-08-09T07:10:34Z"],["dc.date.accessioned","2020-06-25T10:16:52Z"],["dc.date.available","2019-08-09T07:10:34Z"],["dc.date.available","2020-06-25T10:16:52Z"],["dc.date.issued","2018"],["dc.description.abstract","In Drosophila, large variations in rearrangement rate have been reported among different lineages and among Muller's elements. Nevertheless, the mechanisms that are involved in the generation of inversions, their increase in frequency, as well as their impact on the genome are not completely understood. This is in part due to the lack of comparative studies on species distantly related to Drosophila melanogaster. Therefore, we sequenced and assembled the genomes of two species of the virilis phylad (Drosophila novamexicana [15010-1031.00] and Drosophila americana [SF12]), which are diverging from D. melanogaster for more than 40 Myr. Based on these data, we identified the precise location of six novel inversion breakpoints. A molecular characterization provided clear evidence that DAIBAM (a miniature inverted-repeat transposable element) was involved in the generation of eight out of the nine inversions identified. In contrast to what has been previously reported for D. melanogaster and close relatives, ectopic recombination is thus the prevalent mechanism of generating inversions in species of the virilis phylad. Using pool-sequencing data for three populations of D. americana, we also show that common polymorphic inversions create a high degree of genetic differentiation between populations for chromosomes X, 4, and 5 over large physical distances. We did not find statistically significant differences in expression levels between D. americana (SF12) and D. novamexicana (15010-1031.00) strains for the three genes surveyed (CG9588, Fig 4, and fab1) flanking three inversion breakpoints."],["dc.identifier.doi","10.1093/gbe/evy239"],["dc.identifier.pmid","30376068"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16345"],["dc.identifier.scopus","2-s2.0-85060237162"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62355"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66745"],["dc.identifier.url","http://www.scopus.com/inward/record.url?eid=2-s2.0-85060237162&partnerID=MN8TOARS"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1759-6653"],["dc.relation.issn","1759-6653"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Origin and Consequences of Chromosomal Inversions in the virilis Group of Drosophila"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0138758"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Reis, Micael"],["dc.contributor.author","Valer, Felipe B."],["dc.contributor.author","Vieira, Cristina P."],["dc.contributor.author","Vieira, Jorge"],["dc.date.accessioned","2020-06-29T06:52:28Z"],["dc.date.available","2020-06-29T06:52:28Z"],["dc.date.issued","2015"],["dc.description.abstract","Diapause is a period of arrested development which is controlled physiologically, preprogrammed environmentally and characterized by metabolic depression that can occur during any stage of insect development. Nevertheless, in the genus Drosophila, diapause is almost always associated with the cessation of ovarian development and reproductive activity in adult females. In this work, we show that, in D. americana (a temperate species of the virilis group), diapause is a genetically determined delay in ovarian development that is triggered by temperature and/or photoperiod. Moreover, we show that in this species diapause incidence increases with latitude, ranging from 13% in the southernmost to 91% in the northernmost range of the distribution. When exposed to diapause inducing conditions, both diapausing and non-diapausing females show a 10% increase in lifespan, that is further increased by 18.6% in diapausing females, although senescence is far from being negligible.ActinD1 expression levels suggest that diapausing females are biologically much younger than their chronological age, and that the fly as a whole, rather than the ovarian developmental one, which is phenotypically more evident, is delayed by diapause. Therefore, diapause candidate genes that show expression levels that are compatible with flies younger than their chronological age may not necessarily play a role in reproductive diapause and in adaptation to seasonally varying environmental conditions [corrected]."],["dc.identifier.doi","10.1371/journal.pone.0138758"],["dc.identifier.pmid","26398836"],["dc.identifier.scopus","2-s2.0-84946949500"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66756"],["dc.identifier.url","http://www.scopus.com/inward/record.url?eid=2-s2.0-84946949500&partnerID=MN8TOARS"],["dc.language.iso","en"],["dc.relation.eissn","1932-6203"],["dc.relation.issn","1932-6203"],["dc.title","Drosophila Americana diapausing females show features typical of young flies"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e17512"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Reis, Micael"],["dc.contributor.author","Sousa-Guimarães, Sofia"],["dc.contributor.author","Vieira, Cristina P."],["dc.contributor.author","Sunkel, Cláudio E."],["dc.contributor.author","Vieira, Jorge"],["dc.date.accessioned","2020-06-29T07:14:33Z"],["dc.date.available","2020-06-29T07:14:33Z"],["dc.date.issued","2011"],["dc.description.abstract","Using a phylogenetic approach, the examination of 33 meiosis/meiosis-related genes in 12 Drosophila species, revealed nine independent gene duplications, involving the genes cav, mre11, meiS332, polo and mtrm. Evidence is provided that at least eight out of the nine gene duplicates are functional. Therefore, the rate at which Drosophila meiosis/meiosis-related genes are duplicated and retained is estimated to be 0.0012 per gene per million years, a value that is similar to the average for all Drosophila genes. It should be noted that by using a phylogenetic approach the confounding effect of concerted evolution, that is known to lead to overestimation of the duplication and retention rate, is avoided. This is an important issue, since even in our moderate size sample, evidence for long-term concerted evolution (lasting for more than 30 million years) was found for the meiS332 gene pair in species of the Drosophila subgenus. Most striking, in contrast to theoretical expectations, is the finding that genes that encode proteins that must follow a close stoichiometric balance, such as polo, mtrm and meiS332 have been found duplicated. The duplicated genes may be examples of gene neofunctionalization. It is speculated that meiosis duration may be a trait that is under selection in Drosophila and that it has different optimal values in different species."],["dc.identifier.doi","10.1371/journal.pone.0017512"],["dc.identifier.scopus","2-s2.0-79952523294"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66762"],["dc.identifier.url","http://www.scopus.com/inward/record.url?eid=2-s2.0-79952523294&partnerID=MN8TOARS"],["dc.language.iso","en"],["dc.relation.issn","1932-6203"],["dc.title","Drosophila genes that affect meiosis duration are among the meiosis related genes that are more often found duplicated"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","661"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Genome Biology and Evolution"],["dc.bibliographiccitation.lastpage","679"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Fonseca, Nuno A."],["dc.contributor.author","Morales-Hojas, Ramiro"],["dc.contributor.author","Fernandes dos Reis, Micael"],["dc.contributor.author","Rocha, Helder"],["dc.contributor.author","Vieira, Cristina P."],["dc.contributor.author","Nolte, Viola"],["dc.contributor.author","Schlötterer, Christian"],["dc.contributor.author","Vieira, Jorge"],["dc.date.accessioned","2020-06-29T07:12:28Z"],["dc.date.available","2020-06-29T07:12:28Z"],["dc.date.issued","2013"],["dc.description.abstract","Understanding the molecular basis of within and between species phenotypic variation is one of the main goals of Biology. In Drosophila, most of the work regarding this issue has been performed in D. melanogaster, but other distantly related species must also be studied to verify the generality of the findings obtained for this species. Here, we make the case for D. americana, a species of the virilis group of Drosophila that has been diverging from the model species, D. melanogaster, for approximately 40 Myr. To determine the suitability of this species for such studies, polymorphism and recombination estimates are presented for D. americana based on the largest nucleotide sequence polymorphism data set so far analyzed (more than 100 data sets) for this species. The polymorphism estimates are also compared with those obtained from the comparison of the genome assembly of two D. americana strains (H5 and W11) here reported. As an example of the general utility of these resources, we perform a preliminary study on the molecular basis of lifespan differences in D. americana. First, we show that there are lifespan differences between D. americana populations from different regions of the distribution range. Then, we perform five F2 association experiments using markers for 21 candidate genes previously identified in D. melanogaster. Significant associations are found between polymorphism at two genes (hep and Lim3) and lifespan. For the F2 association study involving the two sequenced strains (H5 and W11), we identify amino acid differences at Lim3 and Hep that could be responsible for the observed changes in lifespan. For both genes, no large gene expression differences were observed between the two strains."],["dc.identifier.doi","10.1093/gbe/evt037"],["dc.identifier.pmid","23493635"],["dc.identifier.scopus","2-s2.0-84876523283"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66761"],["dc.identifier.url","http://www.scopus.com/inward/record.url?eid=2-s2.0-84876523283&partnerID=MN8TOARS"],["dc.language.iso","en"],["dc.relation.eissn","1759-6653"],["dc.relation.issn","1759-6653"],["dc.title","Drosophila americana as a model species for comparative studies on the molecular basis of phenotypic variation"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e63747"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Araújo, Ana Rita"],["dc.contributor.author","Reis, Micael"],["dc.contributor.author","Rocha, Helder"],["dc.contributor.author","Aguiar, Bruno"],["dc.contributor.author","Morales-Hojas, Ramiro"],["dc.contributor.author","Macedo-Ribeiro, Sandra"],["dc.contributor.author","Fonseca, Nuno A."],["dc.contributor.author","Reboiro-Jato, David"],["dc.contributor.author","Reboiro-Jato, Miguel"],["dc.contributor.author","Fdez-Riverola, Florentino"],["dc.contributor.author","Vieira, Cristina P."],["dc.contributor.author","Vieira, Jorge"],["dc.date.accessioned","2020-06-29T06:57:44Z"],["dc.date.available","2020-06-29T06:57:44Z"],["dc.date.issued","2013"],["dc.description.abstract","The Drosophila melanogaster G protein-coupled receptor gene, methuselah (mth), has been described as a novel gene that is less than 10 million years old. Nevertheless, it shows a highly specific expression pattern in embryos, larvae, and adults, and has been implicated in larval development, stress resistance, and in the setting of adult lifespan, among others. Although mth belongs to a gene subfamily with 16 members in D. melanogaster, there is no evidence for functional redundancy in this subfamily. Therefore, it is surprising that a novel gene influences so many traits. Here, we explore the alternative hypothesis that mth is an old gene. Under this hypothesis, in species distantly related to D. melanogaster, there should be a gene with features similar to those of mth. By performing detailed phylogenetic, synteny, protein structure, and gene expression analyses we show that the D. virilis GJ12490 gene is the orthologous of mth in species distantly related to D. melanogaster. We also show that, in D. americana (a species of the virilis group of Drosophila), a common amino acid polymorphism at the GJ12490 orthologous gene is significantly associated with developmental time, size, and lifespan differences. Our results imply that GJ12490 orthologous genes are candidates for developmental time and lifespan differences in Drosophila in general."],["dc.identifier.doi","10.1371/journal.pone.0063747"],["dc.identifier.pmid","23696853"],["dc.identifier.scopus","2-s2.0-84877819189"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66758"],["dc.identifier.url","http://www.scopus.com/inward/record.url?eid=2-s2.0-84877819189&partnerID=MN8TOARS"],["dc.language.iso","en"],["dc.relation.eissn","1932-6203"],["dc.title","The Drosophila melanogaster methuselah Gene: A Novel Gene with Ancient Functions"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]