Wilting, Jörg

[["dc.bibliographiccitation.artnumber","157"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cell & Bioscience"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Wilting, Jörg"],["dc.contributor.author","Becker, Jürgen"],["dc.date.accessioned","2022-09-19T07:17:59Z"],["dc.date.available","2022-09-19T07:17:59Z"],["dc.date.issued","2022-09-15"],["dc.date.updated","2022-09-18T03:12:12Z"],["dc.description.abstract","Abstract\r\n Almost 400 years after the (re)discovery of the lymphatic vascular system (LVS) by Gaspare Aselli (Asellius G. De lactibus, sive lacteis venis, quarto vasorum mesaraicorum genere, novo invento Gasparis Asellii Cremo. Dissertatio. (MDCXXIIX), Milan; 1628.), structure, function, development and evolution of this so-called ‘second’ vascular system are still enigmatic. Interest in the LVS was low because it was (and is) hardly visible, and its diseases are not as life-threatening as those of the blood vascular system. It is not uncommon for patients with lymphedema to be told that yes, they can live with it. Usually, the functions of the LVS are discussed in terms of fluid homeostasis, uptake of chylomicrons from the gut, and immune cell circulation. However, the broad molecular equipment of lymphatic endothelial cells suggests that they possess many more functions, which are also reflected in the pathophysiology of the system. With some specific exceptions, lymphatics develop in all organs. Although basic structure and function are the same regardless their position in the body wall or the internal organs, there are important site-specific characteristics. We discuss common structure and function of lymphatics; and point to important functions for hyaluronan turn-over, salt balance, coagulation, extracellular matrix production, adipose tissue development and potential appetite regulation, and the influence of hypoxia on the regulation of these functions. Differences with respect to the embryonic origin and molecular equipment between somatic and splanchnic lymphatics are discussed with a side-view on the phylogeny of the LVS. The functions of the lymphatic vasculature are much broader than generally thought, and lymphatic research will have many interesting and surprising aspects to offer in the future."],["dc.identifier.citation","Cell & Bioscience. 2022 Sep 15;12(1):157"],["dc.identifier.doi","10.1186/s13578-022-00898-0"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114251"],["dc.language.iso","en"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.subject","Initial lymphatics"],["dc.subject","Lymphatic collector"],["dc.subject","Lymphangiogenesis"],["dc.subject","Circulating endothelial precursor cells"],["dc.subject","Pacemaker cell"],["dc.subject","Smooth muscle cell origin"],["dc.subject","Sphingosine-1-phosphate"],["dc.subject","Melanocortin-2 receptor accessory protein-2"],["dc.title","The lymphatic vascular system: much more than just a sewer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1057"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Cellular and Molecular Life Sciences"],["dc.bibliographiccitation.lastpage","1070"],["dc.bibliographiccitation.volume","75"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2020-12-10T14:07:53Z"],["dc.date.available","2020-12-10T14:07:53Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1007/s00018-017-2685-8"],["dc.identifier.eissn","1420-9071"],["dc.identifier.issn","1420-682X"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15539"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70321"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","WNT signaling, the development of the sympathoadrenal–paraganglionic system and neuroblastoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Pregnancy and Childbirth"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Tchagou Tchangou, Gilles E."],["dc.contributor.author","Schmidt, Sonja"],["dc.contributor.author","Zelent, Christina"],["dc.contributor.author","Kahl, Fritz"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2021-04-14T08:25:15Z"],["dc.date.available","2021-04-14T08:25:15Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1186/s12884-020-03073-w"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17437"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81570"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","1471-2393"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Absence of lymphatic vessels in term placenta"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Vascular Cell"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Covelo-Fernandez, Ana"],["dc.contributor.author","von Bonin, Frederike"],["dc.contributor.author","Kube, Dieter"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2021-06-01T10:48:01Z"],["dc.date.available","2021-06-01T10:48:01Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1186/2045-824X-4-3"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7510"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85803"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-824X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Specific tumor-stroma interactions of EBV-positive Burkitt's lymphoma cells in the chick chorioallantoic membrane"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","339"],["dc.bibliographiccitation.journal","BMC Cancer"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Klingenberg, Marcel"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Eberth, Sonja"],["dc.contributor.author","Kube, Dieter"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T09:40:11Z"],["dc.date.available","2018-11-07T09:40:11Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Burkitt lymphoma (BL) is an aggressive malignancy that arises from B-cells and belongs to the group of Non-Hodgkin lymphomas (NHL). Due to the lack of appropriate in vivo models NHL research is mainly performed in vitro. Here, we studied the use of the chick chorioallantoic membrane (CAM) for the generation of human BL xenograft tumors, which we compared with known characteristics of the human disease. Methods: In order to generate experimental BL tumors, we inoculated human BL2B95 and BL2-GFP cells on the CAM. BL2B95 xenograft-tumors were grown for seven days and subsequently analyzed with transmission electron and immunofluorescence microscopy, as well as histological staining approaches. BL2-GFP cells were studied at regular intervals up to seven days, and their metastatic behavior was visualized with intravital immunofluorescence techniques. Results: Xenografted BL2B95 cells formed solid tumors in the CAM model with a Ki67-index greater than 90%, preservation of typical tumor markers (CD10, CD19, CD20), a 'starry sky' morphology, production of agyrophilic fibers in the stroma, formation of blood and lymphatic vessels and lymphogenic dissemination of BL2B95 to distant sites. We identified macrophages, lymphocytes and heterophilic granulocytes (chick homolog of neutrophils) as the most abundant immune cells in the experimental tumors. BL2-GFP cells could be traced in real-time during their distribution in the CAM, and the first signs for their dissemination were visible after 2-3 days. Conclusions: We show that xenografted BL2B95 cells generate tumors in the CAM with a high degree of cellular, molecular and proliferative concord with the human disease, supporting the application of the CAM model for NHL research with a focus on tumor-stroma interactions. Additionally we report that BL2-GFP cells, grafted on the CAM of ex ovo cultured chick embryos, provide a powerful tool to study lymphogenic dissemination in real-time."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [FOR942/12-1, GRK1034]"],["dc.identifier.doi","10.1186/1471-2407-14-339"],["dc.identifier.isi","000338141900001"],["dc.identifier.pmid","24884418"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10149"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33449"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2407"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","The chick chorioallantoic membrane as an in vivo xenograft model for Burkitt lymphoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","763"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Thrombosis and Haemostasis"],["dc.bibliographiccitation.lastpage","773"],["dc.bibliographiccitation.volume","97"],["dc.contributor.author","Ebenebe, Chinedu U."],["dc.contributor.author","Diehl, Stefanie"],["dc.contributor.author","Bartnick, Katja"],["dc.contributor.author","Doerge, Hilmar"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Schweigerer, Lothar L."],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T11:03:04Z"],["dc.date.available","2018-11-07T11:03:04Z"],["dc.date.issued","2007"],["dc.description.abstract","Vascular malformations affect 3% of neonates. Venous malformations (VMs) are the largest group representing more than 50% of cases. In hereditary forms of VMs gene mutations have been identified,but for the large group of spontaneous forms the primary cause and downstream dysregulated genes are unknown. We have performed a global comparison of gene expression in slow-flow VMs and normal saphenous veins using human whole genome micro-arrays. Genes of interest were validated with qRT-PCR. Gene expression in the tunica media was studied after laser micro-dissection of small pieces of tissue. Protein expression in endothelial cells (ECs) was studied with antibodies. We detected 511 genes more than four-fold down- and 112 genes more than four-fold up-regulated. Notably, chemokines, growth factors, transcription factors and regulators of extra-cellular matrix (ECM) turnover were regulated. We observed activation and \"arterialization\" of ECs of the VM proper, whereas ECs of vasa vasorum exhibited up-regulation of inflammation markers. In the tunica media, an altered ECM turnover and composition was found. Our studies demonstrate dysregulated gene expression in tunica interna, media and externa of VMs, and show that each of the three layers represents a reactive compartment. The dysregulated genes may serve as therapeutic targets."],["dc.identifier.doi","10.1160/TH07-01-0021"],["dc.identifier.isi","000246688200011"],["dc.identifier.pmid","17479187"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51533"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Schattauer Gmbh-verlag Medizin Naturwissenschaften"],["dc.relation.issn","0340-6245"],["dc.title","Three reactive compartments in venous malformations"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","365"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","376"],["dc.bibliographiccitation.volume","381"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2021-06-01T10:49:58Z"],["dc.date.available","2021-06-01T10:49:58Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1016/j.ydbio.2013.06.028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86479"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0012-1606"],["dc.title","Maldevelopment of dermal lymphatics in Wnt5a-knockout-mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","681"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","International Journal of Oncology"],["dc.bibliographiccitation.lastpage","689"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","BECKER, JÜRGEN"],["dc.contributor.author","FRÖHLICH, JOHANNA"],["dc.contributor.author","PERSKE, CHRISTINA"],["dc.contributor.author","PAVLAKOVIC, HELENA"],["dc.contributor.author","WILTING, JÖRG"],["dc.date.accessioned","2021-06-01T10:48:52Z"],["dc.date.available","2021-06-01T10:48:52Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.3892/ijo.2012.1488"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86082"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1791-2423"],["dc.relation.issn","1019-6439"],["dc.title","Reelin signalling in neuroblastoma: Migratory switch in metastatic stages"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","209"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cell and Tissue Research"],["dc.bibliographiccitation.lastpage","220"],["dc.bibliographiccitation.volume","330"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Tripodi, Marco"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T10:55:36Z"],["dc.date.available","2018-11-07T10:55:36Z"],["dc.date.issued","2007"],["dc.description.abstract","The homeobox transcription factor Prox1 is expressed in embryonic hepatoblasts and remains expressed in adult hepatocytes. Prox1-null mice show severe deficiencies in liver development, although the underlying mechanisms are unknown. We have studied the effects of Prox1 on the transcriptional profile of met-murine hepatocytes (MMH) obtained on embryonic day 14 (ED14). These immortalized murine hepatoblasts express numerous hepatoblast markers, but not Prox1. We have performed stable transfection with Prox1 cDNA, analyzed the transcriptome with Agilent mouse whole-genome microarrays, and validated genes by quantitative reverse transcription/polymerase chain reaction. We have observed the up-regulation of 22 genes and the down-regulation of 232 genes, by more than 12-fold. Many of these genes are involved in metabolic hepatocyte functions and may be regulated by Prox1 directly or indirectly, e.g., by the down-regulation of hepatocyte nuclear factor 4 alpha. Prox1 induces the down-regulation of transcription factors that are highly expressed in neighboring endodermal organs, suggesting a function during hepatoblast commitment. Prox1 does not influence the proliferative activity of MMH but regulates genes involved in liver morphogenesis. We have observed the up-regulation of both type-IV alpha 3 procollagen and functionally active matrix metalloproteinase-2 (MMP-2), an observation that places Prox1 at the center of liver matrix turnover. This is consistent with MMP-2 expression in hepatoblasts during liver development and with the persistence of a basal lamina around the liver bud in Prox1-deficient mice. Our studies suggest that Prox1 is a multifunctional regulator of liver morphogenesis and of hepatocyte function and commitment."],["dc.identifier.doi","10.1007/s00441-007-0477-4"],["dc.identifier.isi","000249917000002"],["dc.identifier.pmid","17828556"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49825"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0302-766X"],["dc.title","Gene regulation by homeobox transcription factor Prox1 in murine hepatoblasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","235"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","International Journal of Oncology"],["dc.bibliographiccitation.lastpage","240"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Volland, Sonja"],["dc.contributor.author","Noskova, Ievgeniia"],["dc.contributor.author","Schramm, Alexander"],["dc.contributor.author","Schweigerer, Lothar L."],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T11:20:06Z"],["dc.date.available","2018-11-07T11:20:06Z"],["dc.date.issued","2008"],["dc.description.abstract","Neuroblastoma is the most frequent solid malignancy of children. The most reliable prognostic factor in neuroblastoma is the amplification status of the MYCN oncogene, but exceptions from this rule have been observed. Recently we have demonstrated that keratoepithelin (BIGH3, TGFBI) expression significantly reduces proliferation and invasion of neuroblastomas in vitro and in vivo. In these experiments, we also observed that tissue factor pathway inhibitor 2 (TFP12, PP5, MSPI), a potent inhibitor of matrix-metalloproteinases, is most prominently up-regulated. As MYCN-amplified neuroblastomas are highly invasive, we sought to determine the interaction between MYCN, keratoepithelin and TFP12. In this study we provide initial evidence that i) keratoepithelin expression in neuroblastoma inversely correlates with MYCN expression; ii) TFP12 expression in neuroblastoma also correlates inversely with MYCN expression but positively with keratoepithelin expression and iii) keratoepithelin induces elevated TFP12 transcript levels in neuroblastoma cells without alterations of MYCN expression."],["dc.identifier.isi","000251977900026"],["dc.identifier.pmid","18097563"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55457"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Professor D A Spandidos"],["dc.relation.issn","1019-6439"],["dc.title","Keratoepithelin reverts the suppression of tissue factor pathway inhibitor 2 by MYCN in human neuroblastoma: A mechanism to inhibit invasion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]