Wilting, Jörg

[["dc.bibliographiccitation.firstpage","163"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Angiogenesis"],["dc.bibliographiccitation.lastpage","172"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Hemmen, Katherina"],["dc.contributor.author","Reinl, Tobias"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Behler, Friederike"],["dc.contributor.author","Dieken, Hauke"],["dc.contributor.author","Jaensch, Lothar"],["dc.contributor.author","Wilting, Joerg"],["dc.contributor.author","Weich, Herbert A."],["dc.date.accessioned","2018-11-07T08:56:20Z"],["dc.date.available","2018-11-07T08:56:20Z"],["dc.date.issued","2011"],["dc.description.abstract","Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogeneis."],["dc.identifier.doi","10.1007/s10456-011-9200-x"],["dc.identifier.isi","000291038200006"],["dc.identifier.pmid","21234671"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23121"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0969-6970"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","High-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","220"],["dc.bibliographiccitation.lastpage","229"],["dc.contributor.author","Wilting, Jörg"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Rössler, Jochen"],["dc.contributor.author","Norgall, Susanne"],["dc.contributor.author","Schweigerer, Lothar"],["dc.contributor.author","Weich, Herbert A."],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.editor","Chadwick, Derek J."],["dc.contributor.editor","Goode, Jamie"],["dc.date.accessioned","2021-06-02T10:44:30Z"],["dc.date.available","2021-06-02T10:44:30Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1002/9780470319413.ch17"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87064"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","John Wiley & Sons, Ltd"],["dc.publisher.place","Chichester, UK"],["dc.relation.eisbn","978-0-470-31941-3"],["dc.relation.isbn","978-0-470-03428-6"],["dc.relation.ispartof","Vascular Development"],["dc.title","Embryonic Development and Malformation of Lymphatic Vessels"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4581"],["dc.bibliographiccitation.issue","34"],["dc.bibliographiccitation.journal","Current Medicinal Chemistry"],["dc.bibliographiccitation.lastpage","4592"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Wilting, J."],["dc.contributor.author","Becker, J."],["dc.contributor.author","Buttler, K."],["dc.contributor.author","Weich, H."],["dc.date.accessioned","2021-06-01T10:48:33Z"],["dc.date.available","2021-06-01T10:48:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Inflammation is a local or systemic tissue reaction caused by external or internal stimuli with the objective to remove the noxa, inhibit its further dissemination and eventually repair damaged tissue. Blood vessels and perivascular connective tissue are important regulators of the inflammatory process. After a short initial ischemic phase, inflamed tissue is characterized by hyperaemia and increased permeability of capillaries. Therefore, blood vessels have been in the focus of inflammation research for quite some time, whereas lymphatic vessels have been neglected. Their reactivity is not immediately obvious, and, their identification within the tissue has hardly been possible until lymphatic endothelial cell (LEC)-specific molecules have been identified a few years ago. This has opened up the possibility to study lymphatics in normal and diseased tissues, and to isolate LECs for transcriptome and proteome analyses. Initial studies now provide evidence that lymphatics are not just a passive route for circulating lymphocytes, but seem to be directly involved in both the induction and the resolution of inflammation. This review provides a summary on the basics of inflammation, the structure of lymphatics and their molecular markers, human inflammation-associated diseases and their relation to lymphatics, animal models to study the interaction of lymphatics and inflammation, and finally inflammation-associated molecules expressed in LECs. The integration of lymphatics into inflammation research opens up an exciting new field with great clinical potential."],["dc.identifier.doi","10.2174/092986709789760751"],["dc.identifier.isi","000271382900006"],["dc.identifier.pmid","19903150"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85979"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Bentham Science Publ Ltd"],["dc.relation.issn","0929-8673"],["dc.relation.issn","1875-533X"],["dc.title","Lymphatics and Inflammation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","50"],["dc.bibliographiccitation.journal","BMC Cell Biology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schniedermann, Judith"],["dc.contributor.author","Rennecke, Moritz"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Richter, Georg"],["dc.contributor.author","Staedtler, Anna-Maria"],["dc.contributor.author","Norgall, Susanne"],["dc.contributor.author","Badar, Muhammad"],["dc.contributor.author","Barleon, Bernhard"],["dc.contributor.author","May, Tobias"],["dc.contributor.author","Wilting, Joerg"],["dc.contributor.author","Weich, Herbert A."],["dc.date.accessioned","2018-11-07T08:41:30Z"],["dc.date.available","2018-11-07T08:41:30Z"],["dc.date.issued","2010"],["dc.description.abstract","Background: Postnatal endothelial progenitor cells (EPCs) have been successfully isolated from whole bone marrow, blood and the walls of conduit vessels. They can, therefore, be classified into circulating and resident progenitor cells. The differentiation capacity of resident lung endothelial progenitor cells from mouse has not been evaluated. Results: In an attempt to isolate differentiated mature endothelial cells from mouse lung we found that the lung contains EPCs with a high vasculogenic capacity and capability of de novo vasculogenesis for blood and lymph vessels. Mouse lung microvascular endothelial cells (MLMVECs) were isolated by selection of CD31(+) cells. Whereas the majority of the CD31+ cells did not divide, some scattered cells started to proliferate giving rise to large colonies (> 3000 cells/colony). These highly dividing cells possess the capacity to integrate into various types of vessels including blood and lymph vessels unveiling the existence of local microvascular endothelial progenitor cells (LMEPCs) in adult mouse lung. EPCs could be amplified > passage 30 and still expressed panendothelial markers as well as the progenitor cell antigens, but not antigens for immune cells and hematopoietic stem cells. A high percentage of these cells are also positive for Lyve1, Prox1, podoplanin and VEGFR-3 indicating that a considerabe fraction of the cells are committed to develop lymphatic endothelium. Clonogenic highly proliferating cells from limiting dilution assays were also bipotent. Combined in vitro and in vivo spheroid and matrigel assays revealed that these EPCs exhibit vasculogenic capacity by forming functional blood and lymph vessels. Conclusion: The lung contains large numbers of EPCs that display commitment for both types of vessels, suggesting that lung blood and lymphatic endothelial cells are derived from a single progenitor cell."],["dc.identifier.doi","10.1186/1471-2121-11-50"],["dc.identifier.isi","000282731800001"],["dc.identifier.pmid","20594323"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5672"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19484"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2121"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","365"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","376"],["dc.bibliographiccitation.volume","381"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2021-06-01T10:49:58Z"],["dc.date.available","2021-06-01T10:49:58Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1016/j.ydbio.2013.06.028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86479"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0012-1606"],["dc.title","Maldevelopment of dermal lymphatics in Wnt5a-knockout-mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","17"],["dc.bibliographiccitation.lastpage","24"],["dc.contributor.author","Wilting, Jörg"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.editor","Rosen, Steven T."],["dc.contributor.editor","Leong, Stanley P. L."],["dc.date.accessioned","2021-06-02T10:44:20Z"],["dc.date.available","2021-06-02T10:44:20Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1007/978-0-387-69219-7_2"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87003"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","Springer US"],["dc.publisher.place","Boston, MA"],["dc.relation.eisbn","978-0-387-69219-7"],["dc.relation.isbn","978-0-387-69218-0"],["dc.relation.ispartof","Cancer Metastasis And The Lymphovascular System: Basis For Rational Therapy"],["dc.title","Embryonic Development of the Lymphovascular System and Tumor Lymphangiogenesis"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","112"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Pediatric Research"],["dc.bibliographiccitation.lastpage","117"],["dc.bibliographiccitation.volume","68"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Wang, Baigang"],["dc.contributor.author","Pavlakovic, Helena"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2021-06-01T10:48:09Z"],["dc.date.available","2021-06-01T10:48:09Z"],["dc.date.issued","2010"],["dc.identifier.doi","10.1203/PDR.0b013e3181e5bc0f"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85842"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1530-0447"],["dc.relation.issn","0031-3998"],["dc.title","Homeobox Transcription Factor Prox1 in Sympathetic Ganglia of Vertebrate Embryos: Correlation With Human Stage 4s Neuroblastoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2952"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Developmental Dynamics"],["dc.bibliographiccitation.lastpage","2961"],["dc.bibliographiccitation.volume","236"],["dc.contributor.author","Kasten, Philipp"],["dc.contributor.author","Schnoeink, Gerrit"],["dc.contributor.author","Bergmann, Astrid"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Roessler, Jochen"],["dc.contributor.author","Weich, Herbert A."],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T10:57:58Z"],["dc.date.available","2018-11-07T10:57:58Z"],["dc.date.issued","2007"],["dc.description.abstract","Lymphangioma is a disfiguring malformation of early childhood. A mouse lymphangioma model has been established by injecting Freund's incomplete adjuvant (FIA) intraperitoneally, but has not been compared with the human disease. We show that, in accordance with studies from the 1960s, the mouse model represents an oil-granuloma, made up of CD45-positive leukocytes and invaded by blood and lymph vessels. Several markers of lymphatic endothelial cells are expressed in both mouse and human, like CD31, Prox1, podoplanin, and Lyve-1. However, the human disease affects all parts of the lymphovascular tree. We observed convolutes of lymphatic capillaries, irregularly formed collectors with signs of disintegration, and large lymph cysts. We observed VEGFR-2 and -3 expression in both blood vessels and lymphatics of the patients, whereas in mouse VEGFR-2 was confined to activated blood vessels. The experimental mouse FIA model represents a vascularized oil-granuloma rather than a lymphangioma and reflects the complexity of human lymphangioma only partially."],["dc.description.sponsorship","NICHD NIH HHS [N01-HD-6-2915]"],["dc.identifier.doi","10.1002/dvdy.21298"],["dc.identifier.isi","000250192100025"],["dc.identifier.pmid","17879316"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50377"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1058-8388"],["dc.title","Similarities and differences of human and experimental mouse lymphangiomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0164964"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Hasselhof, Viktoria"],["dc.contributor.author","Sperling, Anastasia"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Strobel, Philipp"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Aung, Thiha"],["dc.contributor.author","Felmerer, Gunther"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T10:06:56Z"],["dc.date.available","2018-11-07T10:06:56Z"],["dc.date.issued","2016"],["dc.description.abstract","Millions of patients suffer from lymphedema worldwide. Supporting the contractility of lymphatic collectors is an attractive target for pharmacological therapy of lymphedema. However, lymphatics have mostly been studied in animals, while the cellular and molecular characteristics of human lymphatic collectors are largely unknown. We studied epifascial lymphatic collectors of the thigh, which were isolated for autologous transplantations. Our immunohistological studies identify additional markers for LECs (vimentin, CCBE1). We show and confirm differences between initial and collecting lymphatics concerning the markers ESAM1, D2-40 and LYVE-1. Our transmission electron microscopic studies reveal two types of smooth muscle cells (SMCs) in the media of the collectors with dark and light cytoplasm. We observed vasa vasorum in the media of the largest collectors, as well as interstitial Cajal-like cells, which are highly ramified cells with long processes, caveolae, and lacking a basal lamina. They are in close contact with SMCs, which possess multiple caveolae at the contact sites. Immunohistologically we identified such cells with antibodies against vimentin and PDGFR alpha, but not CD34 and cKIT. With Next Generation Sequencing we searched for highly expressed genes in the media of lymphatic collectors, and found therapeutic targets, suitable for acceleration of lymphatic contractility, such as neuropeptide Y receptors 1, and 5; tachykinin receptors 1, and 2; purinergic receptors P2RX1, and 6, P2RY12, 13, and 14; 5-hydroxytryptamine receptors HTR2B, and 3C; and adrenoceptors alpha(2A),(B),(C). Our studies represent the first comprehensive characterization of human epifascial lymphatic collectors, as a prerequisite for diagnosis and therapy."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0164964"],["dc.identifier.isi","000386204500086"],["dc.identifier.pmid","27764183"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13797"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39190"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Morphological and Molecular Characterization of Human Dermal Lymphatic Collectors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","43"],["dc.bibliographiccitation.journal","BMC Developmental Biology"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Ezaki, Taichi"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T11:15:59Z"],["dc.date.available","2018-11-07T11:15:59Z"],["dc.date.issued","2008"],["dc.description.abstract","Background: The data on the embryonic origin of lymphatic endothelial cells (LECs) from either deep embryonic veins or mesenchymal (or circulating) lymphangioblasts presently available remain inconsistent. In various vertebrates, markers for LECs are first expressed in specific segments of embryonic veins arguing for a venous origin of lymph vessels. Very recently, studies on the mouse have strongly supported this view. However, in the chick, we have observed a dual origin of LECs from veins and from mesodermal lymphangioblasts. Additionally, in murine embryos we have detected mesenchymal cells that co-express LEC markers and the pan-leukocyte marker CD45. Here, we have characterized the mesoderm of murine embryos with LEC markers Prox1, Lyve-1 and LA102 in combination with macrophage markers CD11b and F4/80. Results: We observed cells co-expressing both types of markers (e. g. Prox1 - Lyve-1 - F4/80 triple-positive) located in the mesoderm, immediately adjacent to, and within lymph vessels. Our proliferation studies with Ki-67 antibodies showed high proliferative capacities of both the Lyve-1-positive LECs of lymph sacs/lymphatic sprouts and the Lyve-1-positive mesenchymal cells. Conclusion: Our data argue for a dual origin of LECs in the mouse, although the primary source of embryonic LECs may reside in specific embryonic veins and mesenchymal lymphangioblasts integrated secondarily into lymph vessels. The impact of a dual source of LECs for ontogenetic, phylogenetic and pathological lymphangiogenesis is discussed."],["dc.identifier.doi","10.1186/1471-213X-8-43"],["dc.identifier.isi","000255933200001"],["dc.identifier.pmid","18430230"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54489"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-213X"],["dc.title","Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]