Wilting, Jörg

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","105"],["dc.bibliographiccitation.journal","BMC cancer"],["dc.bibliographiccitation.lastpage","17"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Norgall, Susanne"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Rössler, Jochen"],["dc.contributor.author","Schweigerer, Lothar"],["dc.contributor.author","Wilting, Jörg"],["dc.contributor.author","Weich, Herbert A."],["dc.date.accessioned","2019-07-10T08:13:00Z"],["dc.date.available","2019-07-10T08:13:00Z"],["dc.date.issued","2007"],["dc.description.abstract","Background: Lymphangiomas are neoplasias of childhood. Their etiology is unknown and a causal therapy does not exist. The recent discovery of highly specific markers for lymphatic endothelial cells (LECs) has permitted their isolation and characterization, but expression levels and stability of molecular markers on LECs from healthy and lymphangioma tissues have not been studied yet. We addressed this problem by profiling LECs from normal dermis and two children suffering from lymphangioma, and also compared them with blood endothelial cells (BECs) from umbilical vein, aorta and myometrial microvessels. Methods: Lymphangioma tissue samples were obtained from two young patients suffering from lymphangioma in the axillary and upper arm region. Initially isolated with anti-CD31 (PECAM-1) antibodies, the cells were separated by FACS sorting and magnetic beads using anti-podoplanin and/or LYVE-1 antibodies. Characterization was performed by FACS analysis, immunofluorescence staining, ELISA and micro-array gene analysis. Results: LECs from foreskin and lymphangioma had an almost identical pattern of lymphendothelial markers such as podoplanin, Prox1, reelin, cMaf and integrin-a1 and -a9. However, LYVE-1 was down-regulated and VEGFR-2 and R-3 were up-regulated in lymphangiomas. Prox1 was constantly expressed in LECs but not in any of the BECs. Conclusion: LECs from different sources express slightly variable molecular markers, but can always be distinguished from BECs by their Prox1 expression. High levels of VEGFR-3 and -2 seem to contribute to the etiology of lymphangiomas."],["dc.identifier.fs","171198"],["dc.identifier.ppn","560267541"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4366"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61096"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas"],["dc.title.alternative","Research article"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","220"],["dc.bibliographiccitation.lastpage","229"],["dc.contributor.author","Wilting, Jörg"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Rössler, Jochen"],["dc.contributor.author","Norgall, Susanne"],["dc.contributor.author","Schweigerer, Lothar"],["dc.contributor.author","Weich, Herbert A."],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.editor","Chadwick, Derek J."],["dc.contributor.editor","Goode, Jamie"],["dc.date.accessioned","2021-06-02T10:44:30Z"],["dc.date.available","2021-06-02T10:44:30Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1002/9780470319413.ch17"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87064"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","John Wiley & Sons, Ltd"],["dc.publisher.place","Chichester, UK"],["dc.relation.eisbn","978-0-470-31941-3"],["dc.relation.isbn","978-0-470-03428-6"],["dc.relation.ispartof","Vascular Development"],["dc.title","Embryonic Development and Malformation of Lymphatic Vessels"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","209"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cell and Tissue Research"],["dc.bibliographiccitation.lastpage","220"],["dc.bibliographiccitation.volume","330"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Tripodi, Marco"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T10:55:36Z"],["dc.date.available","2018-11-07T10:55:36Z"],["dc.date.issued","2007"],["dc.description.abstract","The homeobox transcription factor Prox1 is expressed in embryonic hepatoblasts and remains expressed in adult hepatocytes. Prox1-null mice show severe deficiencies in liver development, although the underlying mechanisms are unknown. We have studied the effects of Prox1 on the transcriptional profile of met-murine hepatocytes (MMH) obtained on embryonic day 14 (ED14). These immortalized murine hepatoblasts express numerous hepatoblast markers, but not Prox1. We have performed stable transfection with Prox1 cDNA, analyzed the transcriptome with Agilent mouse whole-genome microarrays, and validated genes by quantitative reverse transcription/polymerase chain reaction. We have observed the up-regulation of 22 genes and the down-regulation of 232 genes, by more than 12-fold. Many of these genes are involved in metabolic hepatocyte functions and may be regulated by Prox1 directly or indirectly, e.g., by the down-regulation of hepatocyte nuclear factor 4 alpha. Prox1 induces the down-regulation of transcription factors that are highly expressed in neighboring endodermal organs, suggesting a function during hepatoblast commitment. Prox1 does not influence the proliferative activity of MMH but regulates genes involved in liver morphogenesis. We have observed the up-regulation of both type-IV alpha 3 procollagen and functionally active matrix metalloproteinase-2 (MMP-2), an observation that places Prox1 at the center of liver matrix turnover. This is consistent with MMP-2 expression in hepatoblasts during liver development and with the persistence of a basal lamina around the liver bud in Prox1-deficient mice. Our studies suggest that Prox1 is a multifunctional regulator of liver morphogenesis and of hepatocyte function and commitment."],["dc.identifier.doi","10.1007/s00441-007-0477-4"],["dc.identifier.isi","000249917000002"],["dc.identifier.pmid","17828556"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49825"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0302-766X"],["dc.title","Gene regulation by homeobox transcription factor Prox1 in murine hepatoblasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","17"],["dc.bibliographiccitation.lastpage","24"],["dc.contributor.author","Wilting, Jörg"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.editor","Rosen, Steven T."],["dc.contributor.editor","Leong, Stanley P. L."],["dc.date.accessioned","2021-06-02T10:44:20Z"],["dc.date.available","2021-06-02T10:44:20Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1007/978-0-387-69219-7_2"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87003"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","Springer US"],["dc.publisher.place","Boston, MA"],["dc.relation.eisbn","978-0-387-69219-7"],["dc.relation.isbn","978-0-387-69218-0"],["dc.relation.ispartof","Cancer Metastasis And The Lymphovascular System: Basis For Rational Therapy"],["dc.title","Embryonic Development of the Lymphovascular System and Tumor Lymphangiogenesis"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2952"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Developmental Dynamics"],["dc.bibliographiccitation.lastpage","2961"],["dc.bibliographiccitation.volume","236"],["dc.contributor.author","Kasten, Philipp"],["dc.contributor.author","Schnoeink, Gerrit"],["dc.contributor.author","Bergmann, Astrid"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Roessler, Jochen"],["dc.contributor.author","Weich, Herbert A."],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T10:57:58Z"],["dc.date.available","2018-11-07T10:57:58Z"],["dc.date.issued","2007"],["dc.description.abstract","Lymphangioma is a disfiguring malformation of early childhood. A mouse lymphangioma model has been established by injecting Freund's incomplete adjuvant (FIA) intraperitoneally, but has not been compared with the human disease. We show that, in accordance with studies from the 1960s, the mouse model represents an oil-granuloma, made up of CD45-positive leukocytes and invaded by blood and lymph vessels. Several markers of lymphatic endothelial cells are expressed in both mouse and human, like CD31, Prox1, podoplanin, and Lyve-1. However, the human disease affects all parts of the lymphovascular tree. We observed convolutes of lymphatic capillaries, irregularly formed collectors with signs of disintegration, and large lymph cysts. We observed VEGFR-2 and -3 expression in both blood vessels and lymphatics of the patients, whereas in mouse VEGFR-2 was confined to activated blood vessels. The experimental mouse FIA model represents a vascularized oil-granuloma rather than a lymphangioma and reflects the complexity of human lymphangioma only partially."],["dc.description.sponsorship","NICHD NIH HHS [N01-HD-6-2915]"],["dc.identifier.doi","10.1002/dvdy.21298"],["dc.identifier.isi","000250192100025"],["dc.identifier.pmid","17879316"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50377"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1058-8388"],["dc.title","Similarities and differences of human and experimental mouse lymphangiomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","451"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","459"],["dc.bibliographiccitation.volume","305"],["dc.contributor.author","Wilting, Joerg"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Schulte, Inga"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Schweigerer, Lothar L."],["dc.contributor.author","Maenner, Joerg"],["dc.date.accessioned","2018-11-07T11:02:22Z"],["dc.date.available","2018-11-07T11:02:22Z"],["dc.date.issued","2007"],["dc.description.abstract","The mass of the myocardium and endocardium of the vertebrate heart derive from the heart-forming fields of the lateral plate mesoderm. Further components of the mature heart such as the epicardium, cardiac interstitium and coronary blood vessels originate from a primarily extracardiac progenitor cell population: the proepicardium (PE). The coronary blood vessels are accompanied by lymph vessels, suggesting a common origin of the two vessel types. However, the origin of cardiac lymphatics has not been studied yet. We have grafted PE of HH-stage 17 (day 3) quail embryos hetero- and hornotopically into chick embryos, which were re-incubated until day 15. Double staining with the quail endothelial cell (EC) marker QH1 and the lymphendothelial marker Prox1 shows that the PE of avian embryos delivers hernangioblasts but not lymphangioblasts. We have never observed quail ECs in lymphatics of the chick host. However, one exception was a large lymphatic trunk at the base of the chick heart, indicating a lympho-venous anastomosis and a 'homing' mechanism of venous ECs into the lymphatic trunk. Cardiac lymphatics grow from the base toward the apex of the heart. In murine embryos, we observed a basal to apical gradient of scattered Lyve-(+)/ CD31(+)/CD45(+) cells in the subepicardium at ernbryonic day 12.5, indicating a contribution of immigrating lymphangioblasts to the cardiac lymphatic system. Our studies show that coronary blood and lymph vessels are derived from different sources, but grow in close association with each other. (c) 2007 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","NICHD NIH HHS [N01-HD-6-2915]"],["dc.identifier.doi","10.1016/j.ydbio.2007.02.026"],["dc.identifier.isi","000246461000007"],["dc.identifier.pmid","17383624"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51369"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0012-1606"],["dc.title","The proepicardium delivers hemangioblasts but not lymphangioblasts to the developing heart"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","549"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","562"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Mansuroglu, Tuemen"],["dc.contributor.author","Batusic, Danko"],["dc.contributor.author","Tron, Kyrylo"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Pieler, Tomas"],["dc.contributor.author","Wilting, Joerg"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T09:01:16Z"],["dc.date.available","2018-11-07T09:01:16Z"],["dc.date.issued","2006"],["dc.description.abstract","The aim of this study was to analyse the changes of Prospero-related homeobox 1 (Prox1) gene expression in rat liver under different experimental conditions of liver injury, regeneration and acute phase reaction, and to correlate it with that of markers for hepatoblasts, hepatocytes, cholangiocytes and oval cells. Gene expression was studied at RNA level by RT-PCR, and at protein level by immunohistochemistry. At embryonal stage of rat liver development (embryonal days (ED) 14-16) hepatoblasts were found to be Prox1(+)/Cytokeratin (CK) 19(+) and alpha-fetoprotein (AFP)(+), at this stage Prox1(-)/CK19(+)/AFP(-) small cells (early cholangiocytes?) were identified. In fetal liver (ED 18-22) hepatoblasts were Prox1(+)/CK19(-)/AFP(+). CK7(+) cholangiocytes were detected at this stage, and they were Prox1(-)/AFP(-). In the adult liver hepatocytes were Prox1(+)/CK19(-)/CK7(-)/AFP(-), cholangiocytes were CK19(+) and/or CK7(+) and AFP(-)/Prox1(-). In models of liver damage and regeneration Prox1 remained a stable marker of hepatocytes. After 2-acetyl-aminofluorene treatment with partial hepatectomy (AAF/PH) the amount of Prox1 specific transcripts was low in the liver, when CK19 and AFP gene expression was high, and at no time point AFP(+)/CK19(+) \"oval cells\" were found to be Prox1(+). However, a few Prox1(+)/CK19(+) and a few Prox1(+)/CK7(+) cells were identified in the liver of AAF/PH-animals, which may represent precursors of hepatocytes, or a precancerous state."],["dc.identifier.doi","10.1007/s00418-006-0191-4"],["dc.identifier.isi","000240980900003"],["dc.identifier.pmid","16770575"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24380"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0948-6143"],["dc.title","Prospero-related homeobox 1 (Prox1) is a stable hepatocyte marker during liver development, injury and regeneration, and is absent from \"oval cells\""],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]