Krause, Petra

[["dc.bibliographiccitation.firstpage","681"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Clinical & Experimental Metastasis"],["dc.bibliographiccitation.lastpage","693"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Flikweert, H."],["dc.contributor.author","Monin, Malte B."],["dc.contributor.author","Hosseini, Ali Seif Amir"],["dc.contributor.author","Helms, G."],["dc.contributor.author","Cantanhede, G."],["dc.contributor.author","Ghadimi, B. Michael"],["dc.contributor.author","Koenig, S."],["dc.date.accessioned","2018-11-07T09:24:13Z"],["dc.date.available","2018-11-07T09:24:13Z"],["dc.date.issued","2013"],["dc.description.abstract","Nearly 50 % of colorectal cancer (CRC) patients develop liver metastases with liver resection being the only option to cure patients. Residual micrometastases or circulating tumor cells are considered a cause of tumor relapse. This work investigates the influence of partial hepatectomy (PH) on the growth and molecular composition of CRC liver metastasis in a syngeneic rat model. One million CC531 colorectal tumor cells were implanted via the portal vein in WAG/Rij rats followed by a 30 % PH a day later. Control groups either received tumor cells followed by a sham-operation or were injected with a buffer solution followed by PH. Animals were examined with magnetic resonance imaging (MRI) and liver tissues were processed for immunolabeling and PCR analysis. One-third PH was associated with an almost threefold increase in relative tumor mass (MRI volumetry: 2.8-fold and transcript levels of CD44: 2.3-fold). Expression of molecular markers for invasiveness and aggressiveness (CD49f, CXCR4, Axin2 and c-met) was increased following PH, however with no significant differences when referring to the relative expression levels (relating to tumor mass). Liver metastases demonstrated a significantly higher proliferation rate (Ki67) 2 weeks following PH and cell divisions also increased in the surrounding liver tissue. Following PH, the stimulated growth of metastases clearly exceeded the compensation in liver volume with long-lasting proliferative effects. However, the distinct tumor composition was not influenced by liver regeneration. Future investigations should focus on the inhibition of cell cycle (i.e. systemic therapy strategies, irradiation) to hinder liver regeneration and therefore restrain tumor growth."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [KO 2218/5-1]"],["dc.identifier.doi","10.1007/s10585-013-9572-y"],["dc.identifier.isi","000319345900013"],["dc.identifier.pmid","23385555"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11172"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29773"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0262-0898"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Increased growth of colorectal liver metastasis following partial hepatectomy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","303"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cell Transplantation"],["dc.bibliographiccitation.lastpage","311"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Yuan, Q."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Kafert-Kasting, S."],["dc.contributor.author","Kriegbaum, H."],["dc.contributor.author","Schneider, Anja"],["dc.contributor.author","Ott, M."],["dc.contributor.author","Meyburg, Jan P."],["dc.date.accessioned","2018-11-07T09:00:56Z"],["dc.date.available","2018-11-07T09:00:56Z"],["dc.date.issued","2011"],["dc.description.abstract","Hepatocyte transplantation is regarded as a promising option to correct hereditary metabolic liver disease. This study describes a novel method involving regional transient portal ischemia (RTPI) in combination with hepatic irradiation (IR) as a preparative regimen for hepatocyte transplantation. The right lobules of rat livers (45% of liver mass) were subjected to RTPI of 30-120 min. Liver specimens and serum samples were analyzed for transaminase levels, DNA damage, apoptosis, and proliferation. Repopulation experiments involved livers of dipeptidylpeptidase IV (DPPIV)-deficient rats preconditioned with RTPI (60-90 min) either with or without prior partial hepatic IR (25 Gy). After reperfusion intervals of 1 and 24 h, 12 million wild-type (DPPIV positive) hepatocytes were transplanted into recipient livers via the spleen. RTPI of 60-90 min caused limited hepatic injury through necrosis and induced a distinct regenerative response in the host Twelve weeks following transplantation, small clusters of donor hepatocytes were detected within the portal areas. Quantitative analysis revealed limited engraftment of 0.79% to 2.95%, whereas control animals (sham OP) exhibited 4.16% (determined as relative activity of DPPIV when compared to wild-type liver). Repopulation was significantly enhanced (21.43%) when IR was performed prior to RTPI, optimum preconditioning settings being 90 min of ischemia and 1 h of reperfusion before transplantation. We demonstrate that RTPI alone is disadvantageous to donor cell engraftment, whereas the combination of IR with RTPI comprises an effective preparative regimen for liver repopulation. The method described clearly has potential for clinical application."],["dc.identifier.doi","10.3727/096368910X520074"],["dc.identifier.isi","000289322000013"],["dc.identifier.pmid","20719089"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7193"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24282"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cognizant Communication Corp"],["dc.relation.issn","0963-6897"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Regional Transient Portal Ischemia and Irradiation as Preparative Regimen for Hepatocyte Transplantation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]