Krause, Petra

[["dc.bibliographiccitation.journal","Strahlentherapie und Onkologie"],["dc.bibliographiccitation.volume","185"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Yuan, Q."],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Wolff, Hendrik Andreas"],["dc.contributor.author","Kafert-Kasting, S."],["dc.contributor.author","Ott, M."],["dc.contributor.author","Meyburg, Jan P."],["dc.contributor.author","Koenig, S."],["dc.date.accessioned","2018-11-07T08:29:44Z"],["dc.date.available","2018-11-07T08:29:44Z"],["dc.date.issued","2009"],["dc.format.extent","144"],["dc.identifier.isi","000268225500376"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16726"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Vogel"],["dc.publisher.place","Munich"],["dc.relation.conference","15th Annual Conference of the Deutschen-Gesellschaft-fur-Radioonkologie"],["dc.relation.eventlocation","Bremen, GERMANY"],["dc.relation.issn","0179-7158"],["dc.title","Liver re-population after radiation and ischemia/reperfusion damage as a proliferation stimulus conditioning in the rat model"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Strahlentherapie und Onkologie"],["dc.bibliographiccitation.volume","183"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Hermann, Robert Michael"],["dc.contributor.author","Becker, H."],["dc.contributor.author","Hess, C. F."],["dc.date.accessioned","2018-11-07T11:02:14Z"],["dc.date.available","2018-11-07T11:02:14Z"],["dc.date.issued","2007"],["dc.format.extent","49"],["dc.identifier.isi","000247071800130"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51330"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Vogel"],["dc.publisher.place","Munich"],["dc.relation.conference","13th Congress of the Deutschen-Gesellschaft-fur-Radioonkologie"],["dc.relation.eventlocation","Hannover, GERMANY"],["dc.relation.issn","0179-7158"],["dc.title","Radiation as a conditioning proliferation stimulus for liver repopulation through transplanted hepatocytes in the rat model - Molecular mechanisms and effect of fractionated radiation"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1115"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Hepatology"],["dc.bibliographiccitation.lastpage","1124"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Drabent, B."],["dc.contributor.author","Schaeffner, I."],["dc.contributor.author","Christ, B."],["dc.contributor.author","Schwartz, P."],["dc.contributor.author","Unthan-Fechner, K."],["dc.contributor.author","Probst, I."],["dc.date.accessioned","2018-11-07T09:45:51Z"],["dc.date.available","2018-11-07T09:45:51Z"],["dc.date.issued","2006"],["dc.description.abstract","Background/Aims: Cultured adult hepatocytes may be stimulated into clonal expansion. We raise the question whether adult hepatocytes proliferating in vitro recapitulate the early process of hepatic development. Methods: A non-enzymatic method was used to isolate hepatocytes free of contamination with non-parenchymal cells. Hepatocytes were stimulated into proliferation in the presence of mitogens and conditioned media from nonparenchymal cell and hepatocyte culture supernatants. Immunofluorescence methods and PCR analysis were used to demonstrate immunophenotypical characteristics and gene expression profiles similar to those of progenitor cells. Results: Rapid growth occurred during the first 7 days of culture. Cells continued to express hepatic markers (phosphoenolpyruvate carboxykinase, cytokeratin 18, transferrin and dipeptidylpeptidase IV), but the gap junction protein connexin 32 was down-regulated. In the early stage of proliferation, cells started to express biliary and extrahepatic progenitor markers (cytokeratin 19, CD49b, CD49f, nestin, vimentin, Thyl and c-kit), followed by cytokeratin 7, connexin 43, and neural cell adhesion molecule. Co-expression of the epithelial liver progenitor marker alpha-foetoprotein with either nestin (neural marker) or Thyl (mesenchymal marker) was also demonstrated. Conclusions: Mature, hepatocytes reveal their potential to regain a spectrum of progenitor markers from different germ layers, suggesting, enormous plasticity and differentiation potential of adult liver cells. (c) 2006 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.jhep.2005.09.016"],["dc.identifier.isi","000237984400014"],["dc.identifier.pmid","16458388"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34724"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0168-8278"],["dc.title","The expression of mesenchymal, neural and haematopoietic stem cell markers in adult hepatocytes proliferating in vitro"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","681"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Clinical & Experimental Metastasis"],["dc.bibliographiccitation.lastpage","693"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Flikweert, H."],["dc.contributor.author","Monin, Malte B."],["dc.contributor.author","Hosseini, Ali Seif Amir"],["dc.contributor.author","Helms, G."],["dc.contributor.author","Cantanhede, G."],["dc.contributor.author","Ghadimi, B. Michael"],["dc.contributor.author","Koenig, S."],["dc.date.accessioned","2018-11-07T09:24:13Z"],["dc.date.available","2018-11-07T09:24:13Z"],["dc.date.issued","2013"],["dc.description.abstract","Nearly 50 % of colorectal cancer (CRC) patients develop liver metastases with liver resection being the only option to cure patients. Residual micrometastases or circulating tumor cells are considered a cause of tumor relapse. This work investigates the influence of partial hepatectomy (PH) on the growth and molecular composition of CRC liver metastasis in a syngeneic rat model. One million CC531 colorectal tumor cells were implanted via the portal vein in WAG/Rij rats followed by a 30 % PH a day later. Control groups either received tumor cells followed by a sham-operation or were injected with a buffer solution followed by PH. Animals were examined with magnetic resonance imaging (MRI) and liver tissues were processed for immunolabeling and PCR analysis. One-third PH was associated with an almost threefold increase in relative tumor mass (MRI volumetry: 2.8-fold and transcript levels of CD44: 2.3-fold). Expression of molecular markers for invasiveness and aggressiveness (CD49f, CXCR4, Axin2 and c-met) was increased following PH, however with no significant differences when referring to the relative expression levels (relating to tumor mass). Liver metastases demonstrated a significantly higher proliferation rate (Ki67) 2 weeks following PH and cell divisions also increased in the surrounding liver tissue. Following PH, the stimulated growth of metastases clearly exceeded the compensation in liver volume with long-lasting proliferative effects. However, the distinct tumor composition was not influenced by liver regeneration. Future investigations should focus on the inhibition of cell cycle (i.e. systemic therapy strategies, irradiation) to hinder liver regeneration and therefore restrain tumor growth."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [KO 2218/5-1]"],["dc.identifier.doi","10.1007/s10585-013-9572-y"],["dc.identifier.isi","000319345900013"],["dc.identifier.pmid","23385555"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11172"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29773"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0262-0898"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Increased growth of colorectal liver metastasis following partial hepatectomy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S310"],["dc.bibliographiccitation.journal","Radiotherapy and Oncology"],["dc.bibliographiccitation.lastpage","S311"],["dc.bibliographiccitation.volume","81"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Kimmina, Sarah"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Hermann, R."],["dc.contributor.author","Becker, H."],["dc.contributor.author","Hess, C."],["dc.date.accessioned","2018-11-07T09:13:49Z"],["dc.date.available","2018-11-07T09:13:49Z"],["dc.date.issued","2006"],["dc.identifier.isi","000242719101075"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27256"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Ireland Ltd"],["dc.publisher.place","Clare"],["dc.relation.eventlocation","Leipzig, GERMANY"],["dc.relation.issn","0167-8140"],["dc.title","Liver repopulation by transplantation of donor hepatocytes after external beam radiotherapy as preparative regimen followed by partial hepatectomy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","303"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cell Transplantation"],["dc.bibliographiccitation.lastpage","311"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Yuan, Q."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Kafert-Kasting, S."],["dc.contributor.author","Kriegbaum, H."],["dc.contributor.author","Schneider, Anja"],["dc.contributor.author","Ott, M."],["dc.contributor.author","Meyburg, Jan P."],["dc.date.accessioned","2018-11-07T09:00:56Z"],["dc.date.available","2018-11-07T09:00:56Z"],["dc.date.issued","2011"],["dc.description.abstract","Hepatocyte transplantation is regarded as a promising option to correct hereditary metabolic liver disease. This study describes a novel method involving regional transient portal ischemia (RTPI) in combination with hepatic irradiation (IR) as a preparative regimen for hepatocyte transplantation. The right lobules of rat livers (45% of liver mass) were subjected to RTPI of 30-120 min. Liver specimens and serum samples were analyzed for transaminase levels, DNA damage, apoptosis, and proliferation. Repopulation experiments involved livers of dipeptidylpeptidase IV (DPPIV)-deficient rats preconditioned with RTPI (60-90 min) either with or without prior partial hepatic IR (25 Gy). After reperfusion intervals of 1 and 24 h, 12 million wild-type (DPPIV positive) hepatocytes were transplanted into recipient livers via the spleen. RTPI of 60-90 min caused limited hepatic injury through necrosis and induced a distinct regenerative response in the host Twelve weeks following transplantation, small clusters of donor hepatocytes were detected within the portal areas. Quantitative analysis revealed limited engraftment of 0.79% to 2.95%, whereas control animals (sham OP) exhibited 4.16% (determined as relative activity of DPPIV when compared to wild-type liver). Repopulation was significantly enhanced (21.43%) when IR was performed prior to RTPI, optimum preconditioning settings being 90 min of ischemia and 1 h of reperfusion before transplantation. We demonstrate that RTPI alone is disadvantageous to donor cell engraftment, whereas the combination of IR with RTPI comprises an effective preparative regimen for liver repopulation. The method described clearly has potential for clinical application."],["dc.identifier.doi","10.3727/096368910X520074"],["dc.identifier.isi","000289322000013"],["dc.identifier.pmid","20719089"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7193"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24282"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cognizant Communication Corp"],["dc.relation.issn","0963-6897"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Regional Transient Portal Ischemia and Irradiation as Preparative Regimen for Hepatocyte Transplantation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","509"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","International Journal of Radiation Oncology*Biology*Physics"],["dc.bibliographiccitation.lastpage","516"],["dc.bibliographiccitation.volume","65"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Hermann, Robert Michael"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Proehl, T."],["dc.contributor.author","Becker, H."],["dc.contributor.author","Hess, C. F."],["dc.contributor.author","Schmidberger, Heinz"],["dc.date.accessioned","2018-11-07T09:45:17Z"],["dc.date.available","2018-11-07T09:45:17Z"],["dc.date.issued","2006"],["dc.description.abstract","Purpose: The transplantation of donor hepatocytes is considered a promising option to correct chronic liver failure through repopulation of the diseased organ. This study describes a novel selective external-beam irradiation technique as a preparative regimen for hepatocyte transplantation. Methods and Materials: Livers of dipeptidylpeptidase IV (DPPIV)-deficient rats were preconditioned with external-beam single-dose irradiation (25 Gy) delivered to two thirds of the liver. Four days later, a one-third partial hepatectomy (PH) was performed to resect the untreated liver section, and 15 million wild-type (DPPIV+) hepatocytes were transplanted via the spleen into the recipient livers. The degree of donor-cell integration and growth was studied 8 h, 3 days, and 5 and 12 weeks after transplantation. Results: Transplanted hepatocytes integrated rapidly into the irradiated liver and proliferated as clusters, finally repopulating the host liver to approximately 20% hepatocyte mass. After 12 weeks, donor cells and their numerous descendents were fully integrated and expressed functional markers to the same extent as host hepatocytes. Conclusions: We demonstrate that external-beam liver irradiation is sufficient to achieve partial repopulation of the host liver after hepatocyte transplantation, under the additional stimulus of one-third PH. The method described has potentially good prospects for its application in a clinically viable form of treatment. (c) 2006 Elsevier Inc."],["dc.identifier.doi","10.1016/j.ijrobp.2006.01.040"],["dc.identifier.isi","000237543800028"],["dc.identifier.pmid","16690433"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34579"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","0360-3016"],["dc.title","External-beam radiotherapy as preparative regimen for hepatocyte transplantation after partial hepatectomy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Strahlentherapie und Onkologie"],["dc.bibliographiccitation.volume","184"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Hermann, Robert Michael"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Becker, H."],["dc.contributor.author","Hess, C. F."],["dc.contributor.author","Koenig, S."],["dc.date.accessioned","2018-11-07T11:15:51Z"],["dc.date.available","2018-11-07T11:15:51Z"],["dc.date.issued","2008"],["dc.format.extent","48"],["dc.identifier.isi","000255034700118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54458"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Vogel"],["dc.publisher.place","Munich"],["dc.relation.conference","14th Annual Congress of the Deutschen-Gesellschaft-fur-Radioonkologie/25th Annual Conference of the Osterreichischen-Gesellschaft-fur-Radioonkologie-Radiobiologie-und-Mediz inische-Radiophysik"],["dc.relation.eventlocation","Vienna, AUSTRIA"],["dc.relation.issn","0179-7158"],["dc.title","Biliary and endothelial side hepatocytic lever re-population after radiation as conditioning proliferation stimulus as well as partial hepatectomy in rat model"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","497"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Cell Transplantation"],["dc.bibliographiccitation.lastpage","506"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Aurich, H."],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Schneider, C."],["dc.contributor.author","Walldorf, J."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Fleig, Wolfgang E."],["dc.contributor.author","Christ, B."],["dc.date.accessioned","2018-11-07T08:35:30Z"],["dc.date.available","2018-11-07T08:35:30Z"],["dc.date.issued","2005"],["dc.description.abstract","Although ex vivo culture of hepatocytes is known to impair functionality, it may still be considered as desirable to propagate or manipulate them in culture prior to transplantation into the host liver. The aim of this study was to clarify whether rat hepatocytes cultured over different periods of time proliferate and retain their hepatocyte-specific functions following transplantation into the recipient liver. Rat hepatocytes were cultured under serum-free conditions in the presence of hepatocyte and epidermal growth factors. Cells derived from wild-type donor livers were transplanted into the livers of CD26-deficient rats. Cell proliferation and the expression of hepatocyte-specific markers were determined before and after transplantation. Cell number increased threefold over a culture period of 10 days. The expression of connexin 32 and phosphoenolpyruvate carboxykinase declined over time, indicating the loss of hepatocyte-specific functions. Hepatocytes cultured over 4 or 7 days and then transplanted proliferated in the host parenchyma. The transplanted cells expressed connexin 32, cytokeratin 18, and phosphoenolpyruvate carboxykinase, indicating the differentiated phenotype. The loss of hepatocyte-specific functions during culture may be restored after transplantation, suggesting that the proper physiological environment is required to maintain the differentiated phenotype."],["dc.identifier.doi","10.3727/000000005783982855"],["dc.identifier.isi","000233025800009"],["dc.identifier.pmid","16285258"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18080"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","0963-6897"],["dc.title","Functional characterization of serum-free cultured rat hepatocytes for downstream transplantation applications"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]