Krause, Petra

[["dc.bibliographiccitation.firstpage","581"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","591"],["dc.bibliographiccitation.volume","135"],["dc.contributor.author","Doratiotto, Silvia"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Serra, Maria Paola"],["dc.contributor.author","Marongiu, Fabio"],["dc.contributor.author","Sini, Marcella"],["dc.contributor.author","Koenig, Sarah"],["dc.contributor.author","Laconi, Ezio"],["dc.date.accessioned","2018-11-07T08:55:35Z"],["dc.date.available","2018-11-07T08:55:35Z"],["dc.date.issued","2011"],["dc.description.abstract","Overt neoplasia is often the end result of a long biological process beginning with the appearance of focal lesions of altered tissue morphology. While the putative clonal nature of focal lesions has often been emphasized, increasing attention is being devoted to the possible role of an altered growth pattern in the evolution of carcinogenesis. Here we compare the growth patterns of normal and nodular hepatocytes in a transplantation system that allows their selective clonal proliferation in vivo. Rats were pre-treated with retrorsine, which blocks the growth of resident hepatocytes, and were then transplanted with hepatocytes isolated from either normal liver or hepatocyte nodules. Both cell types were able to proliferate extensively in the recipient liver, as expected. However, their growth pattern was remarkably different. Clusters of normal hepatocytes integrated in the host liver, displaying a normal histology; however, transplanted nodular hepatocytes formed new hepatocyte nodules, with altered morphology and sharp demarcation from surrounding host liver. Both the expression and distribution of proteins involved in cell polarity, cell communication, and cell adhesion, including connexin 32, E-cadherin, and matrix metalloproteinase-2, were altered in clusters of nodular hepatocytes. Furthermore, we were able to show that down-regulation of connexin 32 and E-cadherin in nodular hepatocyte clusters was independent of growth rate. These results support the concept that a dominant pathway towards neoplastic disease in several organs involves defect(s) in tissue pattern formation."],["dc.description.sponsorship","AIRC (Italian Association for Cancer Research) [4927, 10604]"],["dc.identifier.doi","10.1007/s00418-011-0813-3"],["dc.identifier.isi","000291222300005"],["dc.identifier.pmid","21528371"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6622"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22939"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0948-6143"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The growth pattern of transplanted normal and nodular hepatocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","805"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Cell Transplantation"],["dc.bibliographiccitation.lastpage","817"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Unthan-Fechner, Kirsten"],["dc.contributor.author","Probst, Irmelin"],["dc.contributor.author","Koenig, Sarah"],["dc.date.accessioned","2018-11-07T09:46:06Z"],["dc.date.available","2018-11-07T09:46:06Z"],["dc.date.issued","2014"],["dc.description.abstract","Clinical studies have proved the therapeutic potential of hepatocyte transplantation as a promising alternative to whole organ liver transplantation in the treatment of hereditary or end-stage liver disease. However, donor shortage seriously restricts cell availability, and the lack of appropriate cell culture protocols for the storage and maintenance of donor cells constitutes a significant obstacle. The aim of this study was to stimulate mature hepatocytes in culture to multiply in vitro and track their fate on transplantation. Rat hepatocytes isolated nonenzymatically were cultured serum free for up to 10 days. They were stimulated into proliferation in the presence of growth factors and conditioned media from nonparenchymal and hepatocyte culture supernatants, as well as 10 mM lithium chloride (LiCl). Cell proliferation was assessed by determining DNA content. Additionally, the extent of cell differentiation was estimated using immunofluorescence staining of hepatic, biliary, progenitor, and mesenchymal markers and gene expression analyses. Transplantation studies were performed on the Fischer CD26-mutant rat following pretreatment with retrorsine and partial hepatectomy. Proliferating hepatocytes increasingly adopted precursor characteristics, expressing progenitor (OV6, CD133), hepatic lineage (CK18), biliary (CD49f, CK7, CK19), and mesenchymal (vimentin) markers. The supplement of LiCl further enhanced the proliferative capacity by 30%. Transplantation studies revealed extensive repopulation by large donor hepatocyte clusters. Furthermore, bile duct-like structures deriving from donor cells proved to be immunoreactive to ductular markers and formed in close proximity to endogenous bile ducts. Mature hepatocytes reveal their potential to \"switch\" between phenotypes, adopting progenitor characteristics during proliferation in vitro. Following transplantation, these \"retrodifferentiated\" cells further expanded in vivo, thereby generating bipotentially differentiated progenies (hepatocytes and bile duct-like structures). This apparent plasticity of mature hepatocytes may open new approaches for cell-based strategies to treat liver disease."],["dc.identifier.doi","10.3727/096368913X664856"],["dc.identifier.isi","000337989700002"],["dc.identifier.pmid","23485196"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10644"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34784"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cognizant Communication Corp"],["dc.relation.issn","1555-3892"],["dc.relation.issn","0963-6897"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Cultured Hepatocytes Adopt Progenitor Characteristics and Display Bipotent Capacity to Repopulate the Liver"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","342"],["dc.bibliographiccitation.journal","BMC Cancer"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Bocuk, Derya"],["dc.contributor.author","Wolff, Alexander"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Salinas, Gabriella"],["dc.contributor.author","Bleckmann, Annalen"],["dc.contributor.author","Hackl, Christina"],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Koenig, Sarah"],["dc.date.accessioned","2018-10-10T10:45:50Z"],["dc.date.available","2018-10-10T10:45:50Z"],["dc.date.issued","2017"],["dc.description.abstract","Colorectal cancer (CRC) is the second leading cause of cancer-related death in men and women. Systemic disease with metastatic spread to distant sites such as the liver reduces the survival rate considerably. The aim of this study was to investigate the changes in gene expression that occur on invasion and expansion of CRC cells when forming metastases in the liver. The livers of syngeneic C57BL/6NCrl mice were inoculated with 1 million CRC cells (CMT-93) via the portal vein, leading to the stable formation of metastases within 4 weeks. RNA sequencing performed on the Illumina platform was employed to evaluate the expression profiles of more than 14,000 genes, utilizing the RNA of the cell line cells and liver metastases as well as from corresponding tumour-free liver. A total of 3329 differentially expressed genes (DEGs) were identified when cultured CMT-93 cells propagated as metastases in the liver. Hierarchical clustering on heat maps demonstrated the clear changes in gene expression of CMT-93 cells on propagation in the liver. Gene ontology analysis determined inflammation, angiogenesis, and signal transduction as the top three relevant biological processes involved. Using a selection list, matrix metallopeptidases 2, 7, and 9, wnt inhibitory factor, and chemokine receptor 4 were the top five significantly dysregulated genes. Bioinformatics assists in elucidating the factors and processes involved in CRC liver metastasis. Our results support the notion of an invasion-metastasis cascade involving CRC cells forming metastases on successful invasion and expansion within the liver. Furthermore, we identified a gene expression signature correlating strongly with invasiveness and migration. Our findings may guide future research on novel therapeutic targets in the treatment of CRC liver metastasis."],["dc.identifier.doi","10.1186/s12885-017-3342-1"],["dc.identifier.gro","630797"],["dc.identifier.pmid","28525976"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14459"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15943"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1471-2407"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The adaptation of colorectal cancer cells when forming metastases in the liver: expression of associated genes and pathways in a mouse model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2542"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biomaterials"],["dc.bibliographiccitation.lastpage","2549"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Frosch, Karl-Heinz"],["dc.contributor.author","Drengk, Anja"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Viereckc, V."],["dc.contributor.author","Miosge, Nicolai"],["dc.contributor.author","Werner, C."],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Sturmer, E. K."],["dc.contributor.author","Sturmer, K. M."],["dc.date.accessioned","2018-11-07T10:00:31Z"],["dc.date.available","2018-11-07T10:00:31Z"],["dc.date.issued","2006"],["dc.description.abstract","The goal of the present study was to evaluate the partial surface replacement of the knee with stem cell-coated titanium implants and to provide a basis for a successful treatment of large osteochondral defects. Mesenchymal stem cells (MSCs) were isolated from bone marrow aspirates of adult sheep. Round titanium implants with a diameter of 2 x 7.3 mm were seeded with autologous MSC and inserted into an osteochondral defect in the medial femoral condyle. As controls, defects received either an uncoated implant or were left untreated. Nine animals with 18 defects were sacrificed after 6 months. Histological evaluation was performed by intravital polychrome fluorescent labelling, intravital perfusion with Indian ink, microradiographs and differential staining with toluidine blue. The quality of regenerated cartilage was assessed by in situ hybridization of collagen type II and immunohistochemistry of collagen types I and II. In 50% of the cases, defects treated with MSC-coated implants showed a complete regeneration of the subchondral bone layer. In these cases collagen type II and only traces of collagen type I were detected. A high level of collagen type II mRNA expression compared to articular cartilage indicates regenerating hyaline-like cartilage. A total of 50% of MSC-coated and uncoated implants failed to osseointegrate and formation of fibrocartilage was observed. Untreated defects as well as defects treated with uncoated implants demonstrated incomplete healing of subchondral bone and formation of fibrous cartilage. A modified histological score according to Wakitani significantly demonstrated better results for cell-coated implants (8.8 +/- 6.4) than for uncoated implants (5.5 +/- 3.9) and for untreated defects (2.8 +/- 2.5). Our results demonstrate that, in a significant number of cases, a partial joint resurfacing of the knee with stem cell-coated titanium implants occur. A slow bone and cartilage regeneration and an incomplete healing in half of the MSC-coated implants are limitations of the presented method. To improve our approach and optimize the experimental parameters, further investigations are needed prior to clinical application. (c) 2005 Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.biomaterials.2005.11.034"],["dc.identifier.isi","000235722100004"],["dc.identifier.pmid","16368134"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37824"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","0142-9612"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Stem cell-coated titanium implants for the partial joint resurfacing of the knee"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","681"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Clinical & Experimental Metastasis"],["dc.bibliographiccitation.lastpage","693"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Flikweert, H."],["dc.contributor.author","Monin, Malte B."],["dc.contributor.author","Hosseini, Ali Seif Amir"],["dc.contributor.author","Helms, G."],["dc.contributor.author","Cantanhede, G."],["dc.contributor.author","Ghadimi, B. Michael"],["dc.contributor.author","Koenig, S."],["dc.date.accessioned","2018-11-07T09:24:13Z"],["dc.date.available","2018-11-07T09:24:13Z"],["dc.date.issued","2013"],["dc.description.abstract","Nearly 50 % of colorectal cancer (CRC) patients develop liver metastases with liver resection being the only option to cure patients. Residual micrometastases or circulating tumor cells are considered a cause of tumor relapse. This work investigates the influence of partial hepatectomy (PH) on the growth and molecular composition of CRC liver metastasis in a syngeneic rat model. One million CC531 colorectal tumor cells were implanted via the portal vein in WAG/Rij rats followed by a 30 % PH a day later. Control groups either received tumor cells followed by a sham-operation or were injected with a buffer solution followed by PH. Animals were examined with magnetic resonance imaging (MRI) and liver tissues were processed for immunolabeling and PCR analysis. One-third PH was associated with an almost threefold increase in relative tumor mass (MRI volumetry: 2.8-fold and transcript levels of CD44: 2.3-fold). Expression of molecular markers for invasiveness and aggressiveness (CD49f, CXCR4, Axin2 and c-met) was increased following PH, however with no significant differences when referring to the relative expression levels (relating to tumor mass). Liver metastases demonstrated a significantly higher proliferation rate (Ki67) 2 weeks following PH and cell divisions also increased in the surrounding liver tissue. Following PH, the stimulated growth of metastases clearly exceeded the compensation in liver volume with long-lasting proliferative effects. However, the distinct tumor composition was not influenced by liver regeneration. Future investigations should focus on the inhibition of cell cycle (i.e. systemic therapy strategies, irradiation) to hinder liver regeneration and therefore restrain tumor growth."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [KO 2218/5-1]"],["dc.identifier.doi","10.1007/s10585-013-9572-y"],["dc.identifier.isi","000319345900013"],["dc.identifier.pmid","23385555"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11172"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29773"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0262-0898"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Increased growth of colorectal liver metastasis following partial hepatectomy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","205"],["dc.bibliographiccitation.issue","5-6"],["dc.bibliographiccitation.journal","In Vitro Cellular & Developmental Biology - Animal"],["dc.bibliographiccitation.lastpage","212"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Saghatolislam, Farahnaz"],["dc.contributor.author","Koenig, Sarah"],["dc.contributor.author","Unthan-Fechner, Kirsten"],["dc.contributor.author","Probst, Irmelin"],["dc.date.accessioned","2018-11-07T08:30:19Z"],["dc.date.available","2018-11-07T08:30:19Z"],["dc.date.issued","2009"],["dc.description.abstract","Primary hepatocytes lose their differentiated functions rapidly when in culture. Our aim was to maintain the differentiated status of hepatocytes in vitro by means of vital hepatic stellate cells (HSCs), their soluble and particulate factors and lipid extracts. Hepatocytes were placed into collagen-coated culture dishes in the presence of HSCs at different ages of pre-culture, with or without direct cell to cell contacts, at different cell ratios and in monoculture with cellular HSC components in place of vital cells. Changes in morphology and enhancement of phosphoenolpyruvate carboxykinase (PCK) activity by glucagon were used to determine the differentiated status of hepatocytes in 2d-short-term culture. HSCs proved able to maintain the differentiated function of hepatocytes in co-culture either by direct cell contacts or via factors derived from HSC-conditioned medium. In comparison, however, without cellular contact to hepatocytes five to ten times as many HSCs were necessary to increase the PCK activity to the same degree as in the presence of intercellular contacts. Whereas stimulation in the presence of HSC/hepatocyte contacts was independent of HSC culture age only quiescent, resting HSCs (precultured for 1-2 d) were able to stimulate hepatocytes significantly via soluble factors. Culturing of hepatocytes with a lipid extract or a particulate fraction from HSCs clearly displayed a very strong beneficial effect on enzyme activity and morphology. HSCs maintain hepatocyte function and structure through preferentially cell-bound signalling and transfer of lipids."],["dc.identifier.doi","10.1007/s11626-008-9166-1"],["dc.identifier.isi","000266261700002"],["dc.identifier.pmid","19184253"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3608"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16865"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1071-2690"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Maintaining hepatocyte differentiation in vitro through co-culture with hepatic stellate cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","303"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cell Transplantation"],["dc.bibliographiccitation.lastpage","311"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Koenig, S."],["dc.contributor.author","Yuan, Q."],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Kafert-Kasting, S."],["dc.contributor.author","Kriegbaum, H."],["dc.contributor.author","Schneider, Anja"],["dc.contributor.author","Ott, M."],["dc.contributor.author","Meyburg, Jan P."],["dc.date.accessioned","2018-11-07T09:00:56Z"],["dc.date.available","2018-11-07T09:00:56Z"],["dc.date.issued","2011"],["dc.description.abstract","Hepatocyte transplantation is regarded as a promising option to correct hereditary metabolic liver disease. This study describes a novel method involving regional transient portal ischemia (RTPI) in combination with hepatic irradiation (IR) as a preparative regimen for hepatocyte transplantation. The right lobules of rat livers (45% of liver mass) were subjected to RTPI of 30-120 min. Liver specimens and serum samples were analyzed for transaminase levels, DNA damage, apoptosis, and proliferation. Repopulation experiments involved livers of dipeptidylpeptidase IV (DPPIV)-deficient rats preconditioned with RTPI (60-90 min) either with or without prior partial hepatic IR (25 Gy). After reperfusion intervals of 1 and 24 h, 12 million wild-type (DPPIV positive) hepatocytes were transplanted into recipient livers via the spleen. RTPI of 60-90 min caused limited hepatic injury through necrosis and induced a distinct regenerative response in the host Twelve weeks following transplantation, small clusters of donor hepatocytes were detected within the portal areas. Quantitative analysis revealed limited engraftment of 0.79% to 2.95%, whereas control animals (sham OP) exhibited 4.16% (determined as relative activity of DPPIV when compared to wild-type liver). Repopulation was significantly enhanced (21.43%) when IR was performed prior to RTPI, optimum preconditioning settings being 90 min of ischemia and 1 h of reperfusion before transplantation. We demonstrate that RTPI alone is disadvantageous to donor cell engraftment, whereas the combination of IR with RTPI comprises an effective preparative regimen for liver repopulation. The method described clearly has potential for clinical application."],["dc.identifier.doi","10.3727/096368910X520074"],["dc.identifier.isi","000289322000013"],["dc.identifier.pmid","20719089"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7193"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24282"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cognizant Communication Corp"],["dc.relation.issn","0963-6897"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Regional Transient Portal Ischemia and Irradiation as Preparative Regimen for Hepatocyte Transplantation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3928"],["dc.bibliographiccitation.issue","31"],["dc.bibliographiccitation.journal","World journal of gastroenterology : WJG"],["dc.bibliographiccitation.lastpage","3935"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Rave-Fränk, Margret"],["dc.contributor.author","Wolff, Hendrik Andreas"],["dc.contributor.author","Becker, Heinz"],["dc.contributor.author","Christiansen, Hans"],["dc.contributor.author","Koenig, Sarah"],["dc.date.accessioned","2019-07-10T08:13:36Z"],["dc.date.available","2019-07-10T08:13:36Z"],["dc.date.issued","2010"],["dc.description.abstract","AIM: To investigate whether irradiation (IR) and partial hepatectomy (PH) may prepare the host liver for non-parenchymal cell (NPC) transplantation. METHODS: Livers of dipeptidyl peptidase IV (DPPIV)-deficient rats were pre-conditioned with external beam IR (25 Gy) delivered to two-thirds of the right liver lobules followed by a one-third PH of the untreated lobule. DPPIV-positive liver cells (NPC preparations enriched for liver sinusoidal endothelial cells (LSECs) and hepatocytes) were transplanted via the spleen into the recipient livers. The extent and quality of donor cell engraftment and growth was studied over a long-term interval of 16 wk after transplantation. RESULTS: Host liver staining demonstrated 3 different repopulation types. Well defined clusters of donor-derived hepatocytes with canalicular expression of DPPIV were detectable either adjacent to or in between large areas of donor cells (covering up to 90% of the section plane) co-expressing the endothelial marker platelet endothelial cell adhesion molecule. The third type consisted of formations of DPPIV-positive duct-like structures which co-localized with biliary epithelial CD49f. CONCLUSION: Liver IR and PH as a preconditioning stimulus enables multiple cell liver repopulation by donor hepatocytes, LSECs, and bile duct cells."],["dc.identifier.fs","574471"],["dc.identifier.pmid","20712054"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6866"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61286"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1007-9327"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Bile Ducts"],["dc.subject.mesh","Cell Proliferation"],["dc.subject.mesh","Cell Survival"],["dc.subject.mesh","Dipeptidyl Peptidase 4"],["dc.subject.mesh","Endothelial Cells"],["dc.subject.mesh","Hepatectomy"],["dc.subject.mesh","Hepatocytes"],["dc.subject.mesh","Liver"],["dc.subject.mesh","Liver Regeneration"],["dc.subject.mesh","Rats"],["dc.subject.mesh","Rats, Inbred F344"],["dc.subject.mesh","Rats, Transgenic"],["dc.subject.mesh","Time Factors"],["dc.subject.mesh","Transplantation Conditioning"],["dc.title","Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","37"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","International Journal of Colorectal Disease"],["dc.bibliographiccitation.lastpage","43"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Bobisch, Nina S."],["dc.contributor.author","Thelen, Paul"],["dc.contributor.author","Koehler, Karola"],["dc.contributor.author","Koenig, Sarah"],["dc.contributor.author","Becker, Heinz"],["dc.contributor.author","Leister, Ingo"],["dc.date.accessioned","2018-11-07T09:01:27Z"],["dc.date.available","2018-11-07T09:01:27Z"],["dc.date.issued","2011"],["dc.description.abstract","Laparoscopic surgery in the treatment of colon carcinoma causes pH value alterations as well as changes in fibrinolytic activity. This results in enhanced proliferation of colon carcinoma cells in vitro and also in enhanced growth of liver metastasis when compared to isobaric (gasless) laparoscopy in vivo. So far, the direct influence of CO2 pneumoperitoneum on the invasiveness and metastatic capabilities of colon cancer cells remains unclear. We therefore evaluated transcripts of the uPA system. The influence of CO2 pneumoperitoneum on the gene expression of plasminogen activator inhibitor-1 (PAI-1), urokinase-type plasminogen activator (uPA), and tissue-type plasminogen activator (tPA) was investigated in colon carcinoma cell lines (HT116, SW48, and WiDr) and mesothelial cells employing a pneumoperitoneum chamber in vitro. Quantitative gene expression data were collected using real-time RT-PCR and statistical analysis was performed by means of analysis of variance and Bonferroni correction. The expression of uPA and PAI-1 was increased in colon carcinoma cell lines when cultivated at pH 6.1, a value corresponding to intraabdominal pH values during CO2 insufflation. Elevated PAI-1 mRNA levels were also observed when CO2 was simultaneously applied with a pressure of 10 mmHg. In contrast, there were no significant changes in mesothelial cells in the investigated parameter. The conditions of CO2 pneumoperitoneum cause changes in the expression of genes controlling the fibrinolytic activity. The increase of PAI-1 and uPA can contribute to the enhancement of metastasis and invasive potential of tumour cells. Therefore, changes in the conditions of laparoscopy may well optimise laparoscopic therapy in colon cancer."],["dc.identifier.doi","10.1007/s00384-010-1062-y"],["dc.identifier.isi","000285785600005"],["dc.identifier.pmid","20931209"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6937"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24426"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0179-1958"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The plasminogen activator inhibitor system in colon cancer cell lines is influenced by the CO2 pneumoperitoneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]