Simons, Mikael

[["dc.bibliographiccitation.artnumber","101141"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","STAR Protocols"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Bosch-Queralt, Mar"],["dc.contributor.author","Tiwari, Vini"],["dc.contributor.author","Damkou, Alkmini"],["dc.contributor.author","Vaculčiaková, Lenka"],["dc.contributor.author","Alexopoulos, Ioannis"],["dc.contributor.author","Simons, Mikael"],["dc.date.accessioned","2022-08-19T07:37:21Z"],["dc.date.available","2022-08-19T07:37:21Z"],["dc.date.issued","2022"],["dc.description.abstract","Lysolecithin injections into the white matter tracts of the central nervous system are a valuable tool to study remyelination, but evaluating the resulting demyelinating lesion size is challenging. Here, we present a protocol to consistently measure the volume of demyelination and remyelination in mice following brain lysolecithin injections. We describe serial sectioning of the lesion, followed by the evaluation of the demyelinated area in two-dimensional images. We then detail the computation of the volume using our own automated iPython script. For complete details on the use and execution of this profile, please refer to Bosch-Queralt et al. (2021)."],["dc.identifier.doi","10.1016/j.xpro.2022.101141"],["dc.identifier.pmid","35141565"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113022"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/57"],["dc.language.iso","en"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation.eissn","2666-1667"],["dc.relation.workinggroup","RG Simons (The Biology of Glia in Development and Disease)"],["dc.title","A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Experimental Medicine"],["dc.bibliographiccitation.volume","217"],["dc.contributor.author","Cunha, Maria Inês"],["dc.contributor.author","Su, Minhui"],["dc.contributor.author","Cantuti-Castelvetri, Ludovico"],["dc.contributor.author","Müller, Stephan A."],["dc.contributor.author","Schifferer, Martina"],["dc.contributor.author","Djannatian, Minou"],["dc.contributor.author","Alexopoulos, Ioannis"],["dc.contributor.author","van der Meer, Franziska"],["dc.contributor.author","Winkler, Anne"],["dc.contributor.author","van Ham, Tjakko J."],["dc.contributor.author","Schmid, Bettina"],["dc.contributor.author","Lichtenthaler, Stefan F."],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Simons, Mikael"],["dc.date.accessioned","2020-12-10T18:15:37Z"],["dc.date.available","2020-12-10T18:15:37Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1084/jem.20191390"],["dc.identifier.pmid","32078678"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74902"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/24"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation","TRR 274 | B01: The role of inflammatory cytokine signaling for efficient remyelination in multiple sclerosis"],["dc.relation.workinggroup","RG Cantuti"],["dc.relation.workinggroup","RG Simons (The Biology of Glia in Development and Disease)"],["dc.relation.workinggroup","RG Schifferer"],["dc.relation.workinggroup","RG Stadelmann-Nessler"],["dc.title","Pro-inflammatory activation following demyelination is required for myelin clearance and oligodendrogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","139"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Cell"],["dc.bibliographiccitation.lastpage","151"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Nawaz, Schanila"],["dc.contributor.author","Sanchez, Paula"],["dc.contributor.author","Schmitt, Sebastian"],["dc.contributor.author","Snaidero, Nicolas"],["dc.contributor.author","Mitkovski, Miso"],["dc.contributor.author","Velte, Caroline"],["dc.contributor.author","Brueckner, Bastian Rouven"],["dc.contributor.author","Alexopoulos, Ioannis"],["dc.contributor.author","Czopka, Tim"],["dc.contributor.author","Jung, Sang Y."],["dc.contributor.author","Rhee, Jeong-Seop"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Witke, Walter"],["dc.contributor.author","Schaap, Iwan Alexander Taco"],["dc.contributor.author","Lyons, David A."],["dc.contributor.author","Simons, Mikael"],["dc.date.accessioned","2018-11-07T09:54:28Z"],["dc.date.available","2018-11-07T09:54:28Z"],["dc.date.issued","2015"],["dc.description.abstract","During CNS development, oligodendrocytes wrap their plasma membrane around axons to generate multilamellar myelin sheaths. To drive growth at the leading edge of myelin at the interface with the axon, mechanical forces are necessary, but the underlying mechanisms are not known. Using an interdisciplinary approach that combines morphological, genetic, and biophysical analyses, we identified a key role for actin filament network turnover in myelin growth. At the onset of myelin biogenesis, F-actin is redistributed to the leading edge, where its polymerization-based forces push out non-adhesive and motile protrusions. F-actin disassembly converts protrusions into sheets by reducing surface tension and in turn inducing membrane spreading and adhesion. We identified the actin depolymerizing factor ADF/cofilin1, which mediates high F-actin turnover rates, as an essential factor in this process. We propose that F-actin turnover is the driving force in myelin wrapping by regulating repetitive cycles of leading edge protrusion and spreading."],["dc.identifier.doi","10.1016/j.devcel.2015.05.013"],["dc.identifier.isi","000358599400006"],["dc.identifier.pmid","26166299"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36542"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1878-1551"],["dc.relation.issn","1534-5807"],["dc.title","Actin Filament Turnover Drives Leading Edge Growth during Myelin Sheath Formation in the Central Nervous System"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","211"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nature Metabolism"],["dc.bibliographiccitation.lastpage","227"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Bosch-Queralt, Mar"],["dc.contributor.author","Cantuti-Castelvetri, Ludovico"],["dc.contributor.author","Damkou, Alkmini"],["dc.contributor.author","Schifferer, Martina"],["dc.contributor.author","Schlepckow, Kai"],["dc.contributor.author","Alexopoulos, Ioannis"],["dc.contributor.author","Lütjohann, Dieter"],["dc.contributor.author","Klose, Christian"],["dc.contributor.author","Vaculčiaková, Lenka"],["dc.contributor.author","Masuda, Takahiro"],["dc.contributor.author","Prinz, Marco"],["dc.contributor.author","Monroe, Kathryn M."],["dc.contributor.author","Di Paolo, Gilbert"],["dc.contributor.author","Lewcock, Joseph W."],["dc.contributor.author","Haass, Christian"],["dc.contributor.author","Simons, Mikael"],["dc.date.accessioned","2022-08-18T14:19:20Z"],["dc.date.available","2022-08-18T14:19:20Z"],["dc.date.issued","2021"],["dc.description.abstract","Proregenerative responses are required for the restoration of nervous-system functionality in demyelinating diseases such as multiple sclerosis (MS). Yet, the limiting factors responsible for poor CNS repair are only partially understood. Here, we test the impact of a Western diet (WD) on phagocyte function in a mouse model of demyelinating injury that requires microglial innate immune function for a regenerative response to occur. We find that WD feeding triggers an ageing-related, dysfunctional metabolic response that is associated with impaired myelin-debris clearance in microglia, thereby impairing lesion recovery after demyelination. Mechanistically, we detect enhanced transforming growth factor beta (TGFβ) signalling, which suppresses the activation of the liver X receptor (LXR)-regulated genes involved in cholesterol efflux, thereby inhibiting phagocytic clearance of myelin and cholesterol. Blocking TGFβ or promoting triggering receptor expressed on myeloid cells 2 (TREM2) activity restores microglia responsiveness and myelin-debris clearance after demyelinating injury. Thus, we have identified a druggable microglial immune checkpoint mechanism regulating the microglial response to injury that promotes remyelination."],["dc.identifier.doi","10.1038/s42255-021-00341-7"],["dc.identifier.pmid","33619376"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113011"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/25"],["dc.language.iso","en"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation","TRR 274 | B01: The role of inflammatory cytokine signaling for efficient remyelination in multiple sclerosis"],["dc.relation.issn","2522-5812"],["dc.relation.workinggroup","RG Cantuti"],["dc.relation.workinggroup","RG Schifferer"],["dc.relation.workinggroup","RG Simons (The Biology of Glia in Development and Disease)"],["dc.title","Diet-dependent regulation of TGFβ impairs reparative innate immune responses after demyelination"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]