Fechner, Kim

[["dc.bibliographiccitation.artnumber","e0168733"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Schäfer, Jenny"],["dc.contributor.author","Fechner, Kim"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:43:02Z"],["dc.date.available","2019-07-09T11:43:02Z"],["dc.date.issued","2016"],["dc.description.abstract","BACKGROUND: The detection of Mycobacterium avium subsp. paratuberculosis (MAP) infections in ruminants is crucial to control spread among animals and to humans. Cultivation of MAP is seen as the gold standard for detection, although it is very time consuming and labour intensive. In addition, several PCR assays have been developed to detect MAP in around 90 minutes, but these assays required highly sophisticated equipment as well as lengthy and complicated procedure. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we have developed a rapid assay for the detection of MAP based on the recombinase polymerase amplification (RPA) assay targeting a MAP specific region, the IS900 gene. The detection limit was 16 DNA molecules in 15 minutes as determined by the probit analysis on eight runs of the plasmid standard. Cross reactivity with other mycobacterial and environmentally associated bacterial strains was not observed. The clinical performance of the MAP RPA assay was tested using 48 MAP-positive and 20 MAP-negative blood, sperm, faecal and tissue samples. All results were compared with reads of a highly sensitive real-time PCR assay. The specificity of the MAP RPA assay was 100%, while the sensitivity was 89.5%. CONCLUSIONS/SIGNIFICANCE: The RPA assay is quicker and much easier to handle than real-time PCR. All RPA reagents were cold-chain independent. Moreover, combining RPA assay with a simple extraction protocol will maximize its use at point of need for rapid detection of MAP."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1371/journal.pone.0168733"],["dc.identifier.pmid","27992571"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14082"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58806"],["dc.language.iso","en"],["dc.relation.issn","1932-6203"],["dc.rights.access","openAccess"],["dc.title","Development of a Recombinase Polymerase Amplification Assay for Rapid Detection of the Mycobacterium avium subsp. paratuberculosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","36"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Diagnostics (Basel, Switzerland)"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Roller, Marco"],["dc.contributor.author","Alslim, Lamia M. A."],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Fechner, Kim"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-29T15:02:58Z"],["dc.date.available","2019-07-29T15:02:58Z"],["dc.date.issued","2019"],["dc.description.abstract","The rapid identification of Mycobacteriumavium subspecies paratuberculosis (MAP) infected animals within the herd is essential for preventing the spread of the disease as well as avoiding human exposure. Although culture is seen as the gold standard, there are various molecular assays available i.e., polymerase chain reaction (PCR) or isothermal amplification technique (recombinase polymerase amplification (RPA)) for the detection of MAP. The accuracy of the molecular assays is highly dependent on the DNA extraction method. In order to establish a rapid point of need system for the detection of MAP DNA from stool samples, we developed a rapid DNA extraction protocol (MAP DNA SpeedXtract) specified for use in combination with the RPA. The whole procedure from \"sample in\" to \"result out\" was conducted in a mobile suitcase laboratory. The DNA extraction is based on reverse purification by magnetic beads, which reduces the required technical demand. The MAP DNA SpeedXtract was performed within 25 min and only three pipetting steps were needed. The amplification and detection time were 20 min in RPA. The sensitivity and specificity of the developed protocol in comparison with the lab-based silica membrane column extraction and real-time PCR were 90.9% (n = 22) and 100% (n = 23), respectively. In conclusion, we established a rapid and reliable protocol for the extraction and detection of MAP DNA. All reagents are cold chain independent. The entire setup is ideal for point of need identification of MAP infected cases."],["dc.identifier.doi","10.3390/diagnostics9020036"],["dc.identifier.pmid","30934956"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16310"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62152"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2075-4418"],["dc.relation.issn","2075-4418"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Development of Rapid Extraction Method of Mycobacterium avium Subspecies paratuberculosis DNA from Bovine Stool Samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","28"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Veterinary Sciences"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Lönker, Nelly Sophie"],["dc.contributor.author","Fechner, Kim"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2021-04-14T08:26:58Z"],["dc.date.available","2021-04-14T08:26:58Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/vetsci7010028"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17372"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82129"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2306-7381"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Horses as a Crucial Part of One Health"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]