Schneider, Irene

[["dc.bibliographiccitation.firstpage","1487"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Neurochemistry"],["dc.bibliographiccitation.lastpage","1492"],["dc.bibliographiccitation.volume","75"],["dc.contributor.author","Herms, J. W."],["dc.contributor.author","Korte, S."],["dc.contributor.author","Gall, S."],["dc.contributor.author","Schneider, I."],["dc.contributor.author","Dunker, S."],["dc.contributor.author","Kretzschmar, Hans A."],["dc.date.accessioned","2018-11-07T09:45:16Z"],["dc.date.available","2018-11-07T09:45:16Z"],["dc.date.issued","2000"],["dc.description.abstract","Previous studies have indicated that recombinant cellular prion protein (PrPC), as well as a synthetic peptide of PrPC, affects intracellular calcium homeostasis. To analyze whether calcium homeostasis in neurons lis also affected by a loss of PrPC, we performed microfluorometric calcium measurements on cultured cerebellar granule cells derived from prion protein-deficient (Prnp(0/0)) mice, The resting concentration of intracellular free calcium ([Ca2+](i)) was found to be slightly, but significantly, reduced in Prnp(0/0) mouse granule cell neurites, Moreover, we observed a highly significant reduction in the [Ca2+](i) increase after high potassium depolarization, Pharmacological studies further revealed that the L-type specific blocker nifedipine, which reduces the depolarization-induced [Ca2+](i) increase by 66% in wild-type granule cell somas, has no effect on [Ca2+](i) in Prnp(O/O) mouse granule cells. Patch-clamp measurements, however, did not reveal a reduced calcium influx through voltage-gated calcium channels in Prnp(0/0) mice. These data clearly indicate that loss of PrPC alters the intracellular calcium homeostasis of cultured cerebellar granule cells, There is no evidence, though, that this change is due to a direct alteration of voltage-gated calcium channels."],["dc.identifier.doi","10.1046/j.1471-4159.2000.0751487.x"],["dc.identifier.isi","000089263000016"],["dc.identifier.pmid","10987828"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34575"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0022-3042"],["dc.title","Altered intracellular calcium homeostasis in cerebellar granule cells of prion protein-deficient mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]