Germer, Juliane

[["dc.bibliographiccitation.artnumber","e0140100"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Germer, Juliane"],["dc.contributor.author","Mann, Karlheinz"],["dc.contributor.author","Wörheide, Gert"],["dc.contributor.author","Jackson, Daniel John"],["dc.date.accessioned","2018-11-07T09:49:05Z"],["dc.date.available","2018-11-07T09:49:05Z"],["dc.date.issued","2015"],["dc.description.abstract","The ability to construct a mineralized skeleton was a major innovation for the Metazoa during their evolution in the late Precambrian/early Cambrian. Porifera (sponges) hold an informative position for efforts aimed at unraveling the origins of this ability because they are widely regarded to be the earliest branching metazoans, and are among the first multi-cellular animals to display the ability to biomineralize in the fossil record. Very few biomineralization associated proteins have been identified in sponges so far, with no transcriptome or proteome scale surveys yet available. In order to understand what genetic repertoire may have been present in the last common ancestor of the Metazoa (LCAM), and that may have contributed to the evolution of the ability to biocalcify, we have studied the skeletal proteome of the coralline demosponge Vaceletia sp. and compare this to other metazoan biomineralizing proteomes. We bring some spatial resolution to this analysis by dividing Vaceletia's aragonitic calcium carbonate skeleton into \"head\" and \"stalk\" regions. With our approach we were able to identify 40 proteins from both the head and stalk regions, with many of these sharing some similarity to previously identified gene products from other organisms. Among these proteins are known biomineralization compounds, such as carbonic anhydrase, spherulin, extracellular matrix proteins and very acidic proteins. This report provides the first proteome scale analysis of a calcified poriferan skeletal proteome, and its composition clearly demonstrates that the LCAM contributed several key enzymes and matrix proteins to its descendants that supported the metazoan ability to biocalcify. However, lineage specific evolution is also likely to have contributed significantly to the ability of disparate metazoan lineages to biocalcify."],["dc.description.sponsorship","Open-Access Publikationsfonds 2015"],["dc.identifier.doi","10.1371/journal.pone.0140100"],["dc.identifier.isi","000364298400016"],["dc.identifier.pmid","26536128"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12559"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35436"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","The Skeleton Forming Proteome of an Early Branching Metazoan: A Molecular Survey of the Biomineralization Components Employed by the Coralline Sponge Vaceletia Sp."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","81"],["dc.bibliographiccitation.journal","Frontiers in Marine Science"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Germer, Juliane"],["dc.contributor.author","Cerveau, Nicolas"],["dc.contributor.author","Jackson, Daniel J."],["dc.date.accessioned","2019-07-09T11:43:18Z"],["dc.date.available","2019-07-09T11:43:18Z"],["dc.date.issued","2017"],["dc.description.abstract","djackso@uni-goettingen.de Specialty section: This article was submitted to Aquatic Microbiology, a section of the journal Frontiers in Marine Science Received: 31 October 2016 Accepted: 10 March 2017 Published: 28 March 2017 Citation: Germer J, Cerveau N and Jackson DJ (2017) The Holo-Transcriptome of a Calcified Early Branching Metazoan. Front. Mar. Sci. 4:81. doi: 10.3389/fmars.2017.00081 The Holo-Transcriptome of a Calcified Early Branching Metazoan Juliane Germer, Nicolas Cerveau and Daniel J. Jackson Department of Geobiology, Georg-August University of Göttingen, Göttingen, Germany Symbiotic interactions are widespread throughout the animal kingdom and are increasingly recognized as an important trait that can shape the evolution of a species. Sponges are widely understood to be the earliest branching clade of metazoans and often contain dense, diverse yet specific microbial communities which can constitute up to 50% of their biomass. These bacterial communities fulfill diverse functions influencing the sponge’s physiology and ecology, and may have greatly contributed to the evolutionary success of the Porifera. Here we have analyzed and characterized the holo-transcriptome of the hypercalcifying demosponge Vaceletia sp. and compare it to other sponge transcriptomic and genomic data. Vaceletia sp. harbors a diverse and abundant microbial community; by identifying the underlying molecular mechanism of a variety of lipid pathway components we show that the sponge seems to rely on the supply of short chain fatty acids by its bacterial community. Comparisons to other sponges reveal that this dependency may be more pronounced in sponges with an abundant microbial community. Furthermore, the presence of bacterial polyketide synthase genes suggests bacteria are the producers of Vaceletia’s abundant mid-chain branched fatty acids, whereas demospongic acids may be produced by the sponge host via elongation and desaturation of short-chain precursors. We show that the sponge and its microbial community have the molecular tools to interact through different mechanisms including the sponge’s immune system, and the presence of eukaryotic-like proteins in bacteria. These results expand our knowledge of the complex gene repertoire of sponges and show the importance of metabolic interactions between sponges and their endobiotic microbial communities."],["dc.identifier.doi","10.3389/fmars.2017.00081"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14420"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58857"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2296-7745"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","The Holo-Transcriptome of a Calcified Early Branching Metazoan"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]