Neumann, Bettina

[["dc.bibliographiccitation.firstpage","4798"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","4813"],["dc.bibliographiccitation.volume","47"],["dc.contributor.author","Beissel, Christian"],["dc.contributor.author","Neumann, Bettina"],["dc.contributor.author","Uhse, Simon"],["dc.contributor.author","Hampe, Irene"],["dc.contributor.author","Karki, Prajwal"],["dc.contributor.author","Krebber, Heike"],["dc.date.accessioned","2019-07-25T10:31:21Z"],["dc.date.available","2019-07-25T10:31:21Z"],["dc.date.issued","2019"],["dc.description.abstract","Translation termination requires eRF1 and eRF3 for polypeptide- and tRNA-release on stop codons. Additionally, Dbp5/DDX19 and Rli1/ABCE1 are required; however, their function in this process is currently unknown. Using a combination of in vivo and in vitro experiments, we show that they regulate a stepwise assembly of the termination complex. Rli1 and eRF3-GDP associate with the ribosome first. Subsequently, Dbp5-ATP delivers eRF1 to the stop codon and in this way prevents a premature access of eRF3. Dbp5 dissociates upon placing eRF1 through ATP-hydrolysis. This in turn enables eRF1 to contact eRF3, as the binding of Dbp5 and eRF3 to eRF1 is mutually exclusive. Defects in the Dbp5-guided eRF1 delivery lead to premature contact and premature dissociation of eRF1 and eRF3 from the ribosome and to subsequent stop codon readthrough. Thus, the stepwise Dbp5-controlled termination complex assembly is essential for regular translation termination events. Our data furthermore suggest a possible role of Dbp5/DDX19 in alternative translation termination events, such as during stress response or in developmental processes, which classifies the helicase as a potential drug target for nonsense suppression therapy to treat cancer and neurodegenerative diseases."],["dc.identifier.doi","10.1093/nar/gkz177"],["dc.identifier.pmid","30873535"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16303"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62049"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","1362-4962"],["dc.relation.issn","0305-1048"],["dc.relation.issn","1362-4962"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Translation termination depends on the sequential ribosomal entry of eRF1 and eRF3"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]