Hein, Birka

[["dc.bibliographiccitation.firstpage","8279"],["dc.bibliographiccitation.issue","35"],["dc.bibliographiccitation.journal","Biochemistry"],["dc.bibliographiccitation.lastpage","8281"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Strack, Rita L."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Bhattacharyya, Dibyendu"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Keenan, Robert J."],["dc.contributor.author","Glick, Benjamin S."],["dc.date.accessioned","2017-09-07T11:46:51Z"],["dc.date.available","2017-09-07T11:46:51Z"],["dc.date.issued","2009"],["dc.description.abstract","Fluorescent proteins (FPs) with far-red excitation and emission are desirable for multicolor labeling and live-animal imaging. We describe E2-Crimson, a far-red derivative of the tetrameric FP DsRed-Express2. Unlike other far-red FPs, E2-Crimson is noncytotoxic in bacterial and mammalian cells. E2-Crimson is brighter than other far-red FPs and matures substantially faster than other red and far-red FPs. Approximately 40% of the E2-Crimson fluorescence signal is remarkably photostable. With an excitation maximum at 611 nm, E2-Crimson is the first FP that is efficiently excited with standard far-red lasers. We show that E2-Crimson has unique applications for flow cytometry and stimulated emission depletion (STED) microscopy."],["dc.identifier.doi","10.1021/bi900870u"],["dc.identifier.gro","3143059"],["dc.identifier.isi","000269655600001"],["dc.identifier.pmid","19658435"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/531"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: NIH [R0 I EB008087, T32 BM007183]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-2960"],["dc.title","A Rapidly Maturing Far-Red Derivative of DsRed-Expres2 for Whole-Cell Labeling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","17a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","96"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Nägerl, U. Valentin"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:44:52Z"],["dc.date.available","2022-03-01T11:44:52Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1016/j.bpj.2008.12.988"],["dc.identifier.pii","S0006349508011788"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103148"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy in Living Cells using Live Cell Compatible Markers"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","943"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","945"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Bossi, Mariano"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:48:10Z"],["dc.date.available","2017-09-07T11:48:10Z"],["dc.date.issued","2008"],["dc.description.abstract","We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach."],["dc.identifier.doi","10.1038/nmeth.1257"],["dc.identifier.gro","3143218"],["dc.identifier.isi","000260532500012"],["dc.identifier.pmid","18794861"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/708"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Fluorescence nanoscopy by ground-state depletion and single-molecule return"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","578"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Acta Anaesthesiologica Scandinavica"],["dc.bibliographiccitation.lastpage","579"],["dc.bibliographiccitation.volume","52"],["dc.contributor.author","Schmitto, Jan Dieter"],["dc.contributor.author","Hein, S."],["dc.contributor.author","Braeuer, Anselm"],["dc.contributor.author","Popov, A. F."],["dc.contributor.author","Quintel, M."],["dc.contributor.author","Schoendube, Friedrich Albert"],["dc.date.accessioned","2018-11-07T11:16:15Z"],["dc.date.available","2018-11-07T11:16:15Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1111/j.1399-6576.2008.01580.x"],["dc.identifier.isi","000253982900029"],["dc.identifier.pmid","18339175"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54544"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0001-5172"],["dc.title","Perioperative myocardial infarction after cesarean section in a young woman with hypertrophic obstructive cardiomyopathy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","158"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","163"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:10Z"],["dc.date.available","2017-09-07T11:46:10Z"],["dc.date.issued","2010"],["dc.description.abstract","We show far-field fluorescence nanoscopy of different structural elements labeled with an organic dye within living mammalian cells. The diffraction barrier limiting far-field light microscopy is outperformed by using stimulated emission depletion. We used the tagging protein hAGT (SNAP-tag), which covalently binds benzy1guanine-substituted organic dyes, for labeling. Tetramethy1rhodamine was used to image the cytoskeleton (vimentin and microtubule-associated protein 2) as well as structures located at the cell membrane (caveolin and connexin-43) with a resolution down to 40 nm. Comparison with structures labeled with the yellow fluorescent protein Citrine validates this labeling approach. Nanoscopic movies showing the movement of connexin-43 clusters across the cell membrane evidence the capability of this technique to observe structural changes on the nanoscale over time. Pulsed or continuous-wave lasers for excitation and stimulated emission depletion yield images of similar resolution in living cells. Hence fusion proteins that bind modified organic dyes expand widely the application range of far-field fluorescence nanoscopy of living cells."],["dc.identifier.doi","10.1016/j.bpj.2009.09.053"],["dc.identifier.gro","3142983"],["dc.identifier.isi","000273433800018"],["dc.identifier.pmid","20074516"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/447"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Stimulated Emission Depletion Nanoscopy of Living Cells Using SNAP-Tag Fusion Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1159"],["dc.bibliographiccitation.issue","7233"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","1162"],["dc.bibliographiccitation.volume","457"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Ringemann, Christian"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Schwarzmann, Günter"],["dc.contributor.author","Sandhoff, Konrad"],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","von Middendorff, Claas"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:47:33Z"],["dc.date.available","2017-09-07T11:47:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Cholesterol-mediated lipid interactions are thought to have a functional role in many membrane-associated processes such as signalling events(1-5). Although several experiments indicate their existence, lipid nanodomains ('rafts') remain controversial owing to the lack of suitable detection techniques in living cells(4,6-9). The controversy is reflected in their putative size of 5-200 nm, spanning the range between the extent of a protein complex and the resolution limit of optical microscopy. Here we demonstrate the ability of stimulated emission depletion (STED) far-field fluorescence nanoscopy(10) to detect single diffusing (lipid) molecules in nanosized areas in the plasma membrane of living cells. Tuning of the probed area to spot sizes similar to 70-fold below the diffraction barrier reveals that unlike phosphoglycerolipids, sphingolipids and glycosylphosphatidylinositol-anchored proteins are transiently (similar to 10-20 ms) trapped in cholesterol-mediated molecular complexes dwelling within <20-nm diameter areas. The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells."],["dc.identifier.doi","10.1038/nature07596"],["dc.identifier.gro","3143149"],["dc.identifier.isi","000263680100047"],["dc.identifier.pmid","19098897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/631"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0028-0836"],["dc.title","Direct observation of the nanoscale dynamics of membrane lipids in a living cell"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","197a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","96"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Ringemann, Christian"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:44:51Z"],["dc.date.available","2022-03-01T11:44:51Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1016/j.bpj.2008.12.1058"],["dc.identifier.pii","S0006349508012484"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103138"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","High-Resolution Far-Field Fluorescence STED Microscopy Reveals Nanoscale Details of Molecular Membrane Dynamics"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14271"],["dc.bibliographiccitation.issue","38"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","14276"],["dc.bibliographiccitation.volume","105"],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:12Z"],["dc.date.available","2017-09-07T11:48:12Z"],["dc.date.issued","2008"],["dc.description.abstract","We demonstrate far-field optical imaging with subdiffraction resolution of the endoplasmic reticulum (ER) in the interior of a living mammalian cell. The diffraction barrier is overcome by applying stimulated emission depletion (STED) on a yellow fluorescent protein tag. Imaging individual structural elements of the ER revealed a focal plane (x, y) resolution of < 50 nm inside the living cell, corresponding to a 4-fold improvement over that of a confocal microscope and a 16-fold reduction in the focal-spot cross-sectional area. A similar gain in resolution is realized with both pulsed- and continuous-wave laser illumination. images of highly convoluted parts of the ER reveal a similar resolution improvement in 3D optical sectioning by a factor of 3 along the optic axis (z). Time-lapse STED recordings document morphological changes of the ER over time. Thus, nanoscale 313 imaging of organelles in the interior of living cells greatly expands the scope of light microscopy in cell biology."],["dc.identifier.doi","10.1073/pnas.0807705105"],["dc.identifier.gro","3143235"],["dc.identifier.isi","000259592400010"],["dc.identifier.pmid","18796604"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/727"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: German Ministry for Research and Education"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0027-8424"],["dc.title","Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","580"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","ANASTHESIOLOGIE & INTENSIVMEDIZIN"],["dc.bibliographiccitation.lastpage","584"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Hein, S."],["dc.contributor.author","Benken, I."],["dc.contributor.author","Quintel, M."],["dc.contributor.author","Kettler, Dietrich"],["dc.date.accessioned","2018-11-07T10:44:58Z"],["dc.date.available","2018-11-07T10:44:58Z"],["dc.date.issued","2004"],["dc.description.abstract","The propofol infusion syndrome (PRIS) is a very rare, undesired adverse effect of sedation with propofol that occurs most frequently in children. Despite the fact that a number of fatal cases documented, the syndrome is still relatively unknown. We report the case of a two-year-old boy who was sedated with propofol for 22 hours after surgical resection of a spinal astrocytoma and ultimately died after developing acute rhabdomyolysis with cardiac involvement, severe metabolic acidosis, and acute renal failure. The pathogenesis of PRIS is very probably multifactorial; with various triggering and risk factors being involved to differing extents. A disturbance by propofol of the degradation of free fatty acids resulting in an acute energy deficiency at the cellular level, is considered to be the established cause. In addition, both exogenous and endogenous steroids and/or catecholamines appear to play a role."],["dc.identifier.isi","000224638400006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47392"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","D I O Med Verlags Gmbh"],["dc.relation.issn","0170-5334"],["dc.title","Propofol infusion syndrome - a case report"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","40"],["dc.bibliographiccitation.journal","Biochemistry"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Strack, Rita L."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Bhattacharyya, Dibyendu"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Keenan, Robert J."],["dc.contributor.author","Glick, Benjamin S."],["dc.date.accessioned","2017-09-07T11:46:47Z"],["dc.date.available","2017-09-07T11:46:47Z"],["dc.date.issued","2009"],["dc.format.extent","9704"],["dc.identifier.doi","10.1021/bi901587v"],["dc.identifier.gro","3143043"],["dc.identifier.isi","000270459100041"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/514"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0006-2960"],["dc.title","A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell Labeling (vol 48, pg 8279, 2009)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]