Hein, Birka

[["dc.bibliographiccitation.firstpage","943"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","945"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Bossi, Mariano"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:48:10Z"],["dc.date.available","2017-09-07T11:48:10Z"],["dc.date.issued","2008"],["dc.description.abstract","We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach."],["dc.identifier.doi","10.1038/nmeth.1257"],["dc.identifier.gro","3143218"],["dc.identifier.isi","000260532500012"],["dc.identifier.pmid","18794861"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/708"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Fluorescence nanoscopy by ground-state depletion and single-molecule return"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1159"],["dc.bibliographiccitation.issue","7233"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","1162"],["dc.bibliographiccitation.volume","457"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Ringemann, Christian"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Schwarzmann, Günter"],["dc.contributor.author","Sandhoff, Konrad"],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","von Middendorff, Claas"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:47:33Z"],["dc.date.available","2017-09-07T11:47:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Cholesterol-mediated lipid interactions are thought to have a functional role in many membrane-associated processes such as signalling events(1-5). Although several experiments indicate their existence, lipid nanodomains ('rafts') remain controversial owing to the lack of suitable detection techniques in living cells(4,6-9). The controversy is reflected in their putative size of 5-200 nm, spanning the range between the extent of a protein complex and the resolution limit of optical microscopy. Here we demonstrate the ability of stimulated emission depletion (STED) far-field fluorescence nanoscopy(10) to detect single diffusing (lipid) molecules in nanosized areas in the plasma membrane of living cells. Tuning of the probed area to spot sizes similar to 70-fold below the diffraction barrier reveals that unlike phosphoglycerolipids, sphingolipids and glycosylphosphatidylinositol-anchored proteins are transiently (similar to 10-20 ms) trapped in cholesterol-mediated molecular complexes dwelling within <20-nm diameter areas. The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells."],["dc.identifier.doi","10.1038/nature07596"],["dc.identifier.gro","3143149"],["dc.identifier.isi","000263680100047"],["dc.identifier.pmid","19098897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/631"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0028-0836"],["dc.title","Direct observation of the nanoscale dynamics of membrane lipids in a living cell"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","197a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","96"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Ringemann, Christian"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:44:51Z"],["dc.date.available","2022-03-01T11:44:51Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1016/j.bpj.2008.12.1058"],["dc.identifier.pii","S0006349508012484"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103138"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","High-Resolution Far-Field Fluorescence STED Microscopy Reveals Nanoscale Details of Molecular Membrane Dynamics"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1302"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","1309"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Giske, Arnold"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:46:10Z"],["dc.date.available","2017-09-07T11:46:10Z"],["dc.date.issued","2010"],["dc.description.abstract","We report on fast beam-scanning stimulated-emission-depletion (STED) microscopy in the visible range using for resolution enhancement compact, low cost and turn-key continuous wave (CW) fiber lasers emitting at 592 nm. Spatial resolutions of 35 to 65 nm in the focal plane are shown for various samples including fluorescent nanoparticles, immuno-stained cells with a non-exhaustive selection of 5 commonly used organic fluorescent markers, and living cells expressing the yellow fluorescent protein Citrine. The potential of the straightforward combination of CW-STED and fast beam scanning is illustrated in a movie of the endoplasmic reticulum (ER) of a living cell, composed of 100 frames (6 mu m x 12 mu m), each of them acquired in a time shorter than 0.2 s."],["dc.identifier.doi","10.1364/OE.18.001302"],["dc.identifier.gro","3142981"],["dc.identifier.isi","000273860400098"],["dc.identifier.pmid","20173956"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/444"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","Fast STED microscopy with continuous wave fiber lasers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","721"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","723"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Kellner, Robert R."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:52:34Z"],["dc.date.available","2017-09-07T11:52:34Z"],["dc.date.issued","2006"],["dc.description.abstract","We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The similar to 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers."],["dc.identifier.doi","10.1038/NMETH922"],["dc.identifier.gro","3143638"],["dc.identifier.isi","000240290300016"],["dc.identifier.pmid","16896340"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1174"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Nanoscale resolution in GFP-based microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1784"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","1792"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Boyarskiy, Vadim P."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:48Z"],["dc.date.available","2017-09-07T11:48:48Z"],["dc.date.issued","2008"],["dc.description.abstract","Highly water soluble fluorescent dyes were synthesized and transformed into new amino reactive fluorescent labels for biological microscopy. To this end, rhodamine 8 (prepared from 7-hydroxy-1,2,3,4-tetrahydroquinoline (7) and phthalic anhydride in 85% aq. H(3)PO(4)) was sulfonated with 30% SO(3) in H(2)SO(4) and afforded the water soluble disulfonic acid 3a (64%). Amidation of the carboxy group in 3a with 2-(methylamino)ethanol in the presence of O-(7-azabenzotriazol-1-yl)- N,N,N',N'-tetramethyluronium-PF(6)-(HATU) led to alcohol 3b (66%) which was transformed into the amino reactive mixed carbonate 3d with di(N-succinimidyl)carbonate and Et(3)N. Reaction of the carboxy group in 3a with MeNH(CH(2))(2)CO(2)Me and N,N,N',N'-tetramethyl-O-(N-succinimidyl)-uronium center dot BF(4)(-) (TSTU) yielded methyl ester 13. After saponification of the aliphatic carboxy group in 13, the compound was converted into NHS-ester 3e (using HATU and Et(3)N). Heating of 7 with trimellitic anhydride in H(3)PO(4) gave a mixture of dicarboxylic acids 14 and 15 (1:1). Regioisomer 15 was isolated, sulfonated with 30% SO(3) in H(2)SO(4), and disulfonic acid 3f was used for the synthesis of the mono NHS-ester 3g, in which the sterically unhindered carboxy group was selectively activated (with N-hydroxysuccinimide, HATU, and Et(3)N). The sulfonated rhodamines 3b, c and f are soluble in water (up to 0.1 M), have excellent photostabilities and large fluorescence quantum yields. Subdiffraction resolution images of tubulin filaments of mammalian cells stained with these dyes illustrate their applicability as labels for stimulated emission depletion microscopy and other fluorescence techniques."],["dc.identifier.doi","10.1002/chem.200701058"],["dc.identifier.gro","3143373"],["dc.identifier.isi","000253470900011"],["dc.identifier.pmid","18058955"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/880"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0947-6539"],["dc.title","Photostable, amino reactive and water-soluble fluorescent labels based on sulfonated rhodamine with a rigidized xanthene fragment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]