Lazzara, Thomas Dominic

[["dc.bibliographiccitation.firstpage","911"],["dc.bibliographiccitation.lastpage","940"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.editor","Bhushan, Bharat"],["dc.contributor.editor","Luo, Dan"],["dc.contributor.editor","Schricker, Scott R."],["dc.contributor.editor","Sigmund, Wolfgang"],["dc.contributor.editor","Zauscher, Stefan"],["dc.date.accessioned","2017-09-07T11:54:18Z"],["dc.date.available","2017-09-07T11:54:18Z"],["dc.date.issued","2014"],["dc.description.abstract","Anodic aluminum oxide (AAO) is a nanoporous material with well-defined hexagonally ordered, cylindrical parallel pores running straight through the material’s thickness. Owing to the transparency of AAO, thin-films of this material have been explored for in situ monitoring of biological relevant processes occurring within these nanoporous templates by using optical waveguide spectroscopy (OWS) and fluorescence microscopy."],["dc.identifier.doi","10.1007/978-3-642-31107-9_11"],["dc.identifier.gro","3145142"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2845"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","Springer Nature"],["dc.relation.doi","10.1007/978-3-642-31107-9"],["dc.relation.isbn","978-3-642-31106-2"],["dc.relation.ispartof","Handbook of Nanomaterials Properties"],["dc.title","Biofunctionalization of Nanoporous Alumina Substrates"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4767"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","4774"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Neubacher, Henrik"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Carnarius, Christian"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:46:17Z"],["dc.date.available","2017-09-07T11:46:17Z"],["dc.date.issued","2014"],["dc.description.abstract","Screening tools to study antimicrobial peptides (AMPs) with the aim to optimize therapeutic delivery vectors require automated and parallelized sampling based on chip technology. Here, we present the development of a chip-based assay that allows for the investigation of the action of AMPs on planar lipid membranes in a time-resolved manner by fluorescence readout. Anodic aluminum oxide (AAO) composed of cylindrical pores with a diameter of 70 nm and a thickness of up to 10 mu m was used as a support to generate pore-spanning lipid bilayers from giant unilamellar vesicle spreading, which resulted in large continuous membrane patches sealing the pores. Because AAO is optically transparent, fluid single lipid bilayers and the underlying pore cavities can be readily observed by three-dimensional confocal laser scanning microscopy (CLSM). To assay the permeabilizing activity of the AMPs, the translocation of the water-soluble dyes into the AAO cavities and the fluorescence of the sulforhodamine 101 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanol-l-amine triethylammonium salt (Texas Red DHPE)-labeled lipid membrane were observed by CLSM in a time-resolved manner as a function of the AMP concentration. The effect of two different AMPs, magainin-2 and melittin, was investigated, showing that the concentrations required for membrane permeabilization and the kinetics of the dye entrance differ significantly. Our results are discussed in light of the proposed permeabilization models of the two AMPs. The presented data demonstrate the potential of this setup for the development of an on-chip screening platform for AMPs."],["dc.identifier.doi","10.1021/la500358h"],["dc.identifier.gro","3142140"],["dc.identifier.isi","000335297300029"],["dc.identifier.pmid","24707859"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4988"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft (DFG) [SFB 803]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0743-7463"],["dc.title","Permeabilization Assay for Antimicrobial Peptides Based on Pore-Spanning Lipid Membranes on Nanoporous Alumina"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6935"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","6944"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Carnarius, Christian"],["dc.contributor.author","Kocun, Marta"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:43:25Z"],["dc.date.available","2017-09-07T11:43:25Z"],["dc.date.issued","2011"],["dc.description.abstract","Anodic aluminum oxide (AAO) is a porous material having aligned cylindrical compartments with 55-60 nm diameter pores, and being several micrometers deep. A protocol was developed to generate pore-spanning fluid lipid bilayers separating the attoliter-sized compartments of the nanoporous material from the bulk solution, while preserving the optical transparency of the AAO. The AAO was selectively functionalized by silane chemistry to spread giant unilamellar vesicles (GUVs) resulting In large continuous membrane patches covering the pores. Formation of fluid single lipid bilayers through GUV rupture could be readily observed by fluorescence microscopy and further supported by conservation of membrane surface area, before and after GUV rupture. Fluorescence recovery after photobleaching gale low immobile fractions (5-15%) and lipid diffusion coefficients similar to those found for bilayers on silica. The entrapment of molecules within the porous underlying cylindrical compartments, as well as the exclusion of macromolecules from the nanopores, demonstrate the barrier fun ton of the pore-spanning membranes and could be investigated in three-dimensions using confocal laser scanning fluorescence imaging."],["dc.identifier.doi","10.1021/nn201266e"],["dc.identifier.gro","3142672"],["dc.identifier.isi","000295187400021"],["dc.identifier.pmid","21797231"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/101"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.title","Separating Attoliter-Sized Compartments Using Fluid Pore-Spanning Lipid Bilayers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Abstracts of Papers of the American Chemical Society"],["dc.bibliographiccitation.volume","242"],["dc.contributor.author","Kocun, Marta"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:53:05Z"],["dc.date.available","2018-11-07T08:53:05Z"],["dc.date.issued","2011"],["dc.identifier.isi","000299378301606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22322"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.publisher.place","Washington"],["dc.relation.eventlocation","Denver, CO"],["dc.relation.issn","0065-7727"],["dc.title","Solvent-free, pore-spanning model membranes studied by fluorescence microscopy and atomic force microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Abstracts of Papers of the American Chemical Society"],["dc.bibliographiccitation.volume","242"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Lau, K. H. Aaron"],["dc.contributor.author","Knoll, Wolfgang"],["dc.contributor.author","Majoral, Jean-Pierre"],["dc.contributor.author","Caminade, Anne-Marie"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2018-11-07T08:53:05Z"],["dc.date.available","2018-11-07T08:53:05Z"],["dc.date.issued","2011"],["dc.identifier.isi","000299378301584"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22323"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.publisher.place","Washington"],["dc.relation.eventlocation","Denver, CO"],["dc.relation.issn","0065-7727"],["dc.title","Importance of macromolecular shape and interactions in layer-by-layer assemblies within cylindrical nanopores"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","57"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Colloid and Interface Science"],["dc.bibliographiccitation.lastpage","63"],["dc.bibliographiccitation.volume","366"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Behn, Daniela"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:49:01Z"],["dc.date.available","2017-09-07T11:49:01Z"],["dc.date.issued","2012"],["dc.description.abstract","Anodic aluminum oxide (AAO) substrates with aligned, cylindrical, non-intersecting pores with diameters of 75 nm and depths of 3.5 or 10 mu m were functionalized with lipid monolayers harboring different receptor lipids. AAO was first functionalized with dodecyl-trichlorosilane, followed by fusion of small unilamellar vesicles (SUVs) forming a lipid monolayer. The SUVs' lipid composition was transferred onto the AAO surface, allowing us to control the surface receptor density. Owing to the optical transparency of the MO, the overall vesicle spreading process and subsequent protein binding to the receptor-doped lipid monolayers could be investigated in situ by optical waveguide spectroscopy (OWS). SUV spreading occurred at the pore-rim interface, followed by lateral diffusion of lipids within the pore-interior surface until homogeneous coverage was achieved with a lipid monolayer. The functionality of the system was demonstrated through streptavidin binding onto a biotin-DOPE containing POPC membrane, showing maximum protein coverage at 10 mol% of biotin-DOPE. The system enabled us to monitor in real-time the selective extraction of two histidine-tagged proteins, PIGEA14 (14 kDa) and ezrin (70 kDa), directly from cell lysate solutions using a DOGS-NTA(Ni)/DOPC (1:9) membrane. The purification process including protein binding and elution was monitored by OWS and confirmed by SOS-PAGE. (C) 2011 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.jcis.2011.09.067"],["dc.identifier.gro","3142589"],["dc.identifier.isi","000297385900009"],["dc.identifier.pmid","22033154"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8956"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0021-9797"],["dc.title","Phospholipids as an alternative to direct covalent coupling: Surface functionalization of nanoporous alumina for protein recognition and purification"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7672"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","7680"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Kocun, Marta"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2017-09-07T11:44:09Z"],["dc.date.available","2017-09-07T11:44:09Z"],["dc.date.issued","2011"],["dc.description.abstract","Plasma membrane tension, produced by the underlying cytoskeleton, governs many dynamic processes such as fusion, blebbing exo- and endocytosis, cell migration, and adhesion. Here, a new protocol is introduced to model this intricate and often overlooked aspect of the plasma membrane. Lipid bilayers spanning pores of 600 nm radius were prepared by adsorption and spreading of giant unilamellar vesicles (GUVs) on moderately hydrophilic porous substrates prepared by gold-coating and subsequent self-assembly of a mercaptoethanol monolayer. Rupture of GUVs formed tens of micrometer sized pore-spanning membrane patches displaying low tension of sigma <= 3.5 mN m(-1) and lateral diffusion constants of about 8 mu m(2) s(-1). Site-specific force indentation experiments were performed to determine membrane tension as a function of lipid composition: for pure DOPC bilayers, a tension of 1.018 +/- 0.014 mN m(-1) was measured, which was increased by the addition of cholesterol to 3.50 +/- 0.15 mN m(-1). Compared to DOPC, POPC bilayers displayed a larger tension of 2.00 +/- 0.09 mN m(-1). Addition and subsequent partitioning of 2-propanol was shown to significantly reduce the membrane tension as a function of its concentration."],["dc.identifier.doi","10.1021/la2003172"],["dc.identifier.gro","3142713"],["dc.identifier.isi","000291500700046"],["dc.identifier.pmid","21619014"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/148"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0743-7463"],["dc.title","Preparation of Solvent-Free, Pore-Spanning Lipid Bilayers: Modeling the Low Tension of Plasma Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]