Jablonski, Lukasz Adam

[["dc.bibliographiccitation.firstpage","E3075"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","E3084"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Delvendahl, Igor"],["dc.contributor.author","Jablonski, Lukasz"],["dc.contributor.author","Baade, Carolin"],["dc.contributor.author","Matveev, Victor"],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Hallermann, Stefan"],["dc.date.accessioned","2019-01-24T13:12:31Z"],["dc.date.available","2019-01-24T13:12:31Z"],["dc.date.issued","2015"],["dc.description.abstract","Fast synchronous neurotransmitter release at the presynaptic active zone is triggered by local Ca(2+) signals, which are confined in their spatiotemporal extent by endogenous Ca(2+) buffers. However, it remains elusive how rapid and reliable Ca(2+) signaling can be sustained during repetitive release. Here, we established quantitative two-photon Ca(2+) imaging in cerebellar mossy fiber boutons, which fire at exceptionally high rates. We show that endogenous fixed buffers have a surprisingly low Ca(2+)-binding ratio (∼ 15) and low affinity, whereas mobile buffers have high affinity. Experimentally constrained modeling revealed that the low endogenous buffering promotes fast clearance of Ca(2+) from the active zone during repetitive firing. Measuring Ca(2+) signals at different distances from active zones with ultra-high-resolution confirmed our model predictions. Our results lead to the concept that reduced Ca(2+) buffering enables fast active zone Ca(2+) signaling, suggesting that the strength of endogenous Ca(2+) buffering limits the rate of synchronous synaptic transmission."],["dc.identifier.doi","10.1073/pnas.1508419112"],["dc.identifier.pmid","26015575"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57367"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1091-6490"],["dc.title","Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]