Hémonnot, Clément Y. J.

[["dc.bibliographiccitation.firstpage","1220"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","1223"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Graceffa, Rita"],["dc.contributor.author","Hémonnot, Clément Y. J."],["dc.contributor.author","Ranke, Christiane"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Liebi, Marianne"],["dc.contributor.author","Marmiroli, Benedetta"],["dc.contributor.author","Weihausen, Britta"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2018-02-12T12:27:30Z"],["dc.date.available","2018-02-12T12:27:30Z"],["dc.date.issued","2017"],["dc.description.abstract","Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single‐phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small‐angle X‐ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X‐ray measurements. Second, we solve the problem of “diluting” the sample signal by the signal from the oil separating the emulsion droplets by multiple fast acquisitions per droplet and data thresholding. We show that using our method, even the weakly scattering protein vimentin provides high signal‐to‐noise ratio data."],["dc.identifier.doi","10.1002/cphc.201700221"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/12175"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","molecular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Rapid Acquisition of X-Ray Scattering Data from Droplet-Encapsulated Protein Systems"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3553"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","3561"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hemonnot, Clement Y. J."],["dc.contributor.author","Reinhardt, Juliane"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Patommel, Jens"],["dc.contributor.author","Graceffa, Rita"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Schroer, Christian G."],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:54:36Z"],["dc.date.available","2017-09-07T11:54:36Z"],["dc.date.issued","2016"],["dc.description.abstract","In recent years, X-ray imaging of biological cells has emerged as a complementary alternative to fluorescence and electron microscopy. Different techniques were established and successfully applied to macromolecular assemblies and structures in cells. However, while the resolution is reaching the nanometer scale, the dose is increasing. It is essential to develop strategies to overcome or reduce radiation damage. Here we approach this intrinsic problem by combing two different X-ray techniques, namely ptychography and nanodiffraction, in one experiment and on the same sample. We acquire low dose ptychography overview images of whole cells at a resolution of 65 nm. We subsequently record high-resolution nanodiffraction data from regions of interest. By comparing images from the two modalities, we can exclude strong effects of radiation damage on the specimen. From the diffraction data we retrieve quantitative structural information from intracellular bundles of keratin intermediate filaments such as a filament radius of 5 nm, hexagonal geometric arrangement with an interfilament distance of 14 nm and bundle diameters on the order of 70 nm. Thus, we present an appealing combined approach to answer a broad range of questions in soft matter physics, biophysics and biology."],["dc.identifier.doi","10.1021/acsnano.5b07871"],["dc.identifier.gro","3141720"],["dc.identifier.isi","000372855400058"],["dc.identifier.pmid","26905642"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/324"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","X-rays Reveal the Internal Structure of Keratin Bundles in Whole Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10661"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","10670"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hémonnot, Clément Y. J."],["dc.contributor.author","Ranke, Christiane"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Graceffa, Rita"],["dc.contributor.author","Hagemann, Johannes"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-11-28T10:03:27Z"],["dc.date.available","2017-11-28T10:03:27Z"],["dc.date.issued","2016"],["dc.description.abstract","X-ray imaging of intact biological cells is emerging as a complementary method to visible light or electron microscopy. Owing to the high penetration depth and small wavelength of X-rays, it is possible to resolve subcellular structures at a resolution of a few nanometers. Here, we apply scanning X-ray nanodiffraction in combination with time-lapse bright-field microscopy to nuclei of 3T3 fibroblasts and thus relate the observed structures to specific phases in the cell division cycle. We scan the sample at a step size of 250 nm and analyze the individual diffraction patterns according to a generalized Porod’s law. Thus, we obtain information on the aggregation state of the nuclear DNA at a real space resolution on the order of the step size and in parallel structural information on the order of few nanometers. We are able to distinguish nucleoli, heterochromatin, and euchromatin in the nuclei and follow the compaction and decompaction during the cell division cycle."],["dc.identifier.doi","10.1021/acsnano.6b05034"],["dc.identifier.fs","623722"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/10593"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject","biological cells; cell division cycle; DNA compaction; nanostructure; X-ray nanodiffraction"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.title","Following DNA Compaction During the Cell Cycle by X-ray Nanodiffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","171"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Lab on a Chip"],["dc.bibliographiccitation.lastpage","178"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Denz, Manuela"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Hémonnot, Clément Y. J."],["dc.contributor.author","Spears, Heidi"],["dc.contributor.author","Wittmeier, Andrew"],["dc.contributor.author","Cassini, Chiara"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Perego, Eleonora"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Burghammer, Manfred"],["dc.date.accessioned","2021-06-01T10:50:48Z"],["dc.date.available","2021-06-01T10:50:48Z"],["dc.date.issued","2018"],["dc.description.abstract","The combination of microfluidics and X-ray methods attracts a lot of attention from researchers as it brings together the high controllability of microfluidic sample environments and the small length scales probed by X-rays. In particular, the fields of biophysics and biology have benefited enormously from such approaches. We introduce a straightforward fabrication method for X-ray compatible microfluidic devices made solely from cyclic olefin copolymers. We benchmark the performance of the devices against other devices including more commonly used Kapton windows and obtain data of equal quality using small angle X-ray scattering. An advantage of the devices presented here is that no gluing between interfaces is necessary, rendering the production very reliable. As a biophysical application, we investigate the early time points of the assembly of vimentin intermediate filament proteins into higher-order structures. This weakly scattering protein system leads to high quality data in the new devices, thus opening up the way for numerous future applications."],["dc.identifier.doi","10.1039/C7LC00824D"],["dc.identifier.eissn","1473-0189"],["dc.identifier.issn","1473-0197"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86792"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.relation.eissn","1473-0189"],["dc.relation.issn","1473-0197"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","microfluidics"],["dc.title","Cyclic olefin copolymer as an X-ray compatible material for microfluidic devices"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]