Gunka, Katrin

[["dc.bibliographiccitation.artnumber","1492"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Cascante-Estepa, Nora"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Stuelke, Joerg"],["dc.date.accessioned","2018-11-07T10:08:26Z"],["dc.date.available","2018-11-07T10:08:26Z"],["dc.date.issued","2016"],["dc.description.abstract","In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases, and accessory proteins. In the Gram-positive model organism Bacillus subtilis, the existence of a RNA degradosome assembled around the membrane-bound endoribonuclease RNase Y has been proposed. Here, we have studied the intracellular localization of the protein that have been implicated in the potential B. subtilis RNA degradosome, i.e., polynucleotide phosphorylase, the exoribonucleases J1 and J2, the DEAD-box RNA helicase CshA, and the glycolytic enzymes enolase and phosphofructokinase. Our data suggests that the bulk of these enzymes is located in the cytoplasm. The RNases J1 and J2 as well as the RNA helicase CshA were mainly localized in the peripheral regions of the cell where also the bulk of messenger RNA is localized. We were able to demonstrate active exclusion of these proteins from the transcribing nucleoid. Taken together, our findings suggest that the interactions of the enzymes involved in RNA degradation in B. subtilis are rather transient."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.3389/fmicb.2016.01492"],["dc.identifier.isi","000383647300001"],["dc.identifier.pmid","27708634"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13776"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39460"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Frontiers Media Sa"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Localization of Components of the RNA-Degrading Machine in Bacillus subtilis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","758"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Stannek, Lorena"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Care, Rachel A."],["dc.contributor.author","Gerth, Ulf"],["dc.contributor.author","Commichau, Fabian M."],["dc.date.accessioned","2018-11-07T10:02:18Z"],["dc.date.available","2018-11-07T10:02:18Z"],["dc.date.issued","2015"],["dc.description.abstract","The Gram-positive model bacterium Bacillus subtilis contains two glutamate dehydrogenase-encoding genes, rocG and gudB. While the rocG gene encodes the functional GDH, the gudB gene is cryptic (gudB(CR)) in the laboratory strain 168 due to a perfect 18 bp-long direct repeat that renders the GudB enzyme inactive and unstable. Although constitutively expressed the GudB(CR) protein can hardly be detected in B. subtilis as it is rapidly degraded within stationary growth phase. Its high instability qualifies GudB(CR) as a model substrate for studying protein turnover in B. subtilis. Recently, we have developed a visual screen to monitor the GudB(CR) stability in the cell using a GFP-GudB(CR) fusion. Using fluorescent microscopy we found that the G FP protein is simultaneously degraded together with GudB(CR). This allows us to analyze the stability of GudB(CR) in living cells. By combining the visual screen with a transposon mutagenesis approach we looked for mutants that show an increased fluorescence signal compared to the wild type indicating a stabilized GFP-GudB(CR) fusion. We observed, that disruption of the arginine kinase encoding gene mcsB upon transposon insertion leads to increased amounts of the GFP-GudB(CR) fusion in this mutant. Deletion of the cognate arginine phosphatase YwIE in contrast results in reduced levels of the GFP-GudB(CR) fusion. Recently, it was shown that the kinase McsB is involved in phosphorylation of GudB(CR) on arginine residues. Here we show that selected arginine-lysine point mutations of GudB(CR) exhibit no influence on degradation. The activity of McsB and YwIE, however, are crucial for the activation and inhibition, respectively, of a proteolytic machinery that efficiently degrades the unstable GudB(CR) protein in B. subtilis."],["dc.identifier.doi","10.3389/fmicb.2014.00758"],["dc.identifier.isi","000348706900001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11795"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38197"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Frontiers Media Sa"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Factors that mediate and prevent degradation of the inactive and unstable GudB protein in Bacillus subtilis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Wetzel, Daniela"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Chibani, Cynthia"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Liesegang, Heiko"],["dc.date.accessioned","2019-07-09T11:45:11Z"],["dc.date.available","2019-07-09T11:45:11Z"],["dc.date.issued","2018"],["dc.description.abstract","BACKGROUND: Clostridioides difficile infections (CDI) have emerged over the past decade causing symptoms that range from mild, antibiotic-associated diarrhea (AAD) to life-threatening toxic megacolon. In this study, we describe a multiple and isochronal (mixed) CDI caused by the isolates DSM 27638, DSM 27639 and DSM 27640 that already initially showed different morphotypes on solid media. RESULTS: The three isolates belonging to the ribotypes (RT) 012 (DSM 27639) and 027 (DSM 27638 and DSM 27640) were phenotypically characterized and high quality closed genome sequences were generated. The genomes were compared with seven reference strains including three strains of the RT 027, two of the RT 017, and one of the RT 078 as well as a multi-resistant RT 012 strain. The analysis of horizontal gene transfer events revealed gene acquisition incidents that sort the strains within the time line of the spread of their RTs within Germany. We could show as well that horizontal gene transfer between the members of different RTs occurred within this multiple infection. In addition, acquisition and exchange of virulence-related features including antibiotic resistance genes were observed. Analysis of the two genomes assigned to RT 027 revealed three single nucleotide polymorphisms (SNPs) and apparently a regional genome modification within the flagellar switch that regulates the fli operon. CONCLUSION: Our findings show that (i) evolutionary events based on horizontal gene transfer occur within an ongoing CDI and contribute to the adaptation of the species by the introduction of new genes into the genomes, (ii) within a multiple infection of a single patient the exchange of genetic material was responsible for a much higher genome variation than the observed SNPs."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2018"],["dc.identifier.doi","10.1186/s12864-017-4368-0"],["dc.identifier.pmid","29291715"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59178"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/15123 but duplicate"],["dc.notes.status","final"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Comparative genome and phenotypic analysis of three Clostridioides difficile strains isolated from a single patient provide insight into multiple infection of C. difficile."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1843"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Seugendo, Mwanaisha"],["dc.contributor.author","Janssen, Iryna"],["dc.contributor.author","Lang, Vanessa"],["dc.contributor.author","Hasibuan, Irene"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Kusumawati, R. L."],["dc.contributor.author","Mshana, Stephen E."],["dc.contributor.author","von Müller, Lutz"],["dc.contributor.author","Okamo, Benard"],["dc.contributor.author","Ortlepp, Jan R."],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Groß, Uwe"],["dc.date.accessioned","2019-07-09T11:45:47Z"],["dc.date.available","2019-07-09T11:45:47Z"],["dc.date.issued","2018"],["dc.description.abstract","Clostridioides (Clostridium) difficile infections (CDI) are considered worldwide as emerging health threat. Uptake of C. difficile spores may result in asymptomatic carrier status or lead to CDI that could range from mild diarrhea, eventually developing into pseudomembranous colitis up to a toxic megacolon that often results in high mortality. Most epidemiological studies to date have been performed in middle- and high income countries. Beside others, the use of antibiotics and the composition of the microbiome have been identified as major risk factors for the development of CDI. We therefore postulate that prevalence rates of CDI and the distribution of C. difficile strains differ between geographical regions depending on the regional use of antibiotics and food habits. A total of 593 healthy control individuals and 608 patients suffering from diarrhea in communities in Germany, Ghana, Tanzania and Indonesia were selected for a comparative multi-center cross-sectional study. The study populations were screened for the presence of C. difficile in stool samples. Cultured C. difficile strains (n = 84) were further subtyped and characterized using PCR-ribotyping, determination of toxin production, and antibiotic susceptibility testing. Prevalence rates of C. difficile varied widely between the countries. Whereas high prevalence rates were observed in symptomatic patients living in Germany and Indonesia (24.0 and 14.7%), patients from Ghana and Tanzania showed low detection rates (4.5 and 6.4%). Differences were also obvious for ribotype distribution and toxin repertoires. Toxin A+/B+ ribotypes 001/072 and 078 predominated in Germany, whereas most strains isolated from Indonesian patients belonged to toxin A+/B+ ribotype SLO160 and toxin A-/B+ ribotype 017. With 42.9-73.3%, non-toxigenic strains were most abundant in Africa, but were also found in Indonesia at a rate of 18.2%. All isolates were susceptible to vancomycin and metronidazole. Mirroring the antibiotic use, however, moxifloxacin resistance was absent in African C. difficile isolates but present in Indonesian (24.2%) and German ones (65.5%). This study showed that CDI is a global health threat with geographically different prevalence rates which might reflect distinct use of antibiotics. Significant differences for distributions of ribotypes, toxin production, and antibiotic susceptibilities were observed."],["dc.identifier.doi","10.3389/fmicb.2018.01843"],["dc.identifier.pmid","30131799"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15318"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59311"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Prevalence and Strain Characterization of Clostridioides (Clostridium) difficile in Representative Regions of Germany, Ghana, Tanzania and Indonesia - A Comparative Multi-Center Cross-Sectional Study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","170152"],["dc.bibliographiccitation.journal","Scientific data"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Schneider, Dominik"],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Gollnow, Kathleen"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2019-07-09T11:44:31Z"],["dc.date.available","2019-07-09T11:44:31Z"],["dc.date.issued","2017-10-17"],["dc.description.abstract","We present bacterial 16S rRNA gene datasets derived from stool samples of 44 patients with diarrhea indicative of a Clostridioides difficile infection. For 20 of these patients, C. difficile infection was confirmed by clinical evidence. Stool samples from patients originating from Germany, Ghana, and Indonesia were taken and subjected to DNA isolation. DNA isolations of stool samples from 35 asymptomatic control individuals were performed. The bacterial community structure was assessed by 16S rRNA gene analysis (V3-V4 region). Metadata from patients and control individuals include gender, age, country, presence of diarrhea, concomitant diseases, and results of microbiological tests to diagnose C. difficile presence. We provide initial data analysis and a dataset overview. After processing of paired-end sequencing data, reads were merged, quality-filtered, primer sequences removed, reads truncated to 400 bp and dereplicated. Singletons were removed and sequences were sorted by cluster size, clustered at 97% sequence similarity and chimeric sequences were discarded. Taxonomy to each operational taxonomic unit was assigned by BLASTn searches against Silva database 123.1 and a table was constructed."],["dc.identifier.doi","10.1038/sdata.2017.152"],["dc.identifier.pmid","29039846"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14810"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59030"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2052-4463"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Gut bacterial communities of diarrheic patients with indications of Clostridioides difficile infection."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","99"],["dc.bibliographiccitation.journal","New microbes and new infections"],["dc.bibliographiccitation.lastpage","102"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Seugendo, M."],["dc.contributor.author","Mshana, S. E."],["dc.contributor.author","Hokororo, A."],["dc.contributor.author","Okamo, B."],["dc.contributor.author","Mirambo, M. M."],["dc.contributor.author","von Müller, L."],["dc.contributor.author","Gunka, K."],["dc.contributor.author","Zimmermann, O."],["dc.contributor.author","Groß, U."],["dc.date.accessioned","2019-07-09T11:42:36Z"],["dc.date.available","2019-07-09T11:42:36Z"],["dc.date.issued","2015-11-01"],["dc.description.abstract","Little is known regarding the epidemiology Clostridium difficile in developing countries. Fresh stool samples from patients with diarrhoea were cultured anaerobically. C. difficile was detected in nine (6.4%) of 141 (95% confidence interval 4.2-13.1), of which seven (77.8%) were from children. HIV infection, prolonged hospitalization and antibiotic use were independent factors associated with the occurrence of C. difficile in the gastrointestinal tract. Two of the toxigenic isolates were typed as ribotype 045, and the other two had unknown ribotype. All C. difficile isolates were susceptible to metronidazole, moxifloxacin and clarithromycin, while three isolates were resistant to clarithromycin. C. difficile may be an important pathogen causing diarrhoea in sub-Saharan Africa among immunocompromised patients."],["dc.identifier.doi","10.1016/j.nmni.2015.09.016"],["dc.identifier.fs","617714"],["dc.identifier.pmid","26649183"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13602"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58705"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2052-2975"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","Clostridium difficile infections among adults and children in Mwanza/Tanzania: is it an underappreciated pathogen among immunocompromised patients in sub-Saharan Africa?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e66120"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Stannek, Lorena"],["dc.contributor.author","Care, Rachel A."],["dc.contributor.author","Commichau, Fabian M."],["dc.date.accessioned","2018-11-07T09:23:41Z"],["dc.date.available","2018-11-07T09:23:41Z"],["dc.date.issued","2013"],["dc.description.abstract","Soil bacteria like Bacillus subtilis can cope with many growth conditions by adjusting gene expression and metabolic pathways. Alternatively, bacteria can spontaneously accumulate beneficial mutations or shape their genomes in response to stress. Recently, it has been observed that a B. subtilis mutant lacking the catabolically active glutamate dehydrogenase (GDH), RocG, mutates the cryptic gudB(CR) gene at a high frequency. The suppressor mutants express the active GDH GudB, which can fully replace the function of RocG. Interestingly, the cryptic gudB(CR) allele is stably inherited as long as the bacteria synthesize the functional GDH RocG. Competition experiments revealed that the presence of the cryptic gudB(CR) allele provides the bacteria with a selective growth advantage when glutamate is scarce. Moreover, the lack of exogenous glutamate is the driving force for the selection of mutants that have inactivated the active gudB gene. In contrast, two functional GDHs are beneficial for the cells when glutamate was available. Thus, the amount of GDH activity strongly affects fitness of the bacteria depending on the availability of exogenous glutamate. At a first glance the high mutation frequency of the cryptic gudB(CR) allele might be attributed to stress-induced adaptive mutagenesis. However, other loci on the chromosome that could be potentially mutated during growth under the selective pressure that is exerted on a GDH-deficient mutant remained unaffected. Moreover, we show that a GDH-proficient B. subtilis strain has a strong selective growth advantage in a glutamate-dependent manner. Thus, the emergence and rapid clonal expansion of the active gudB allele can be in fact explained by spontaneous mutation and growth under selection without an increase of the mutation rate. Moreover, this study shows that the selective pressure that is exerted on a maladapted bacterium strongly affects the apparent mutation frequency of mutational hot spots."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2013"],["dc.identifier.doi","10.1371/journal.pone.0066120"],["dc.identifier.isi","000321038800048"],["dc.identifier.pmid","23785476"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9123"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29637"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Selection-Driven Accumulation of Suppressor Mutants in Bacillus subtilis: The Apparent High Mutation Frequency of the Cryptic gudB Gene and the Rapid Clonal Expansion of gudB(+) Suppressors Are Due to Growth under Selection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","5"],["dc.bibliographiccitation.journal","BMC Systems Biology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Florez, Lope A."],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Polania, Rafael"],["dc.contributor.author","Tholen, Stefan"],["dc.contributor.author","Stuelke, Joerg"],["dc.date.accessioned","2018-11-07T09:00:06Z"],["dc.date.available","2018-11-07T09:00:06Z"],["dc.date.issued","2011"],["dc.description.abstract","Background: Several computational methods exist to suggest rational genetic interventions that improve the productivity of industrial strains. Nonetheless, these methods are less effective to predict possible genetic responses of the strain after the intervention. This problem requires a better understanding of potential alternative metabolic and regulatory pathways able to counteract the targeted intervention. Results: Here we present SPABBATS, an algorithm based on Boolean satisfiability (SAT) that computes alternative metabolic pathways between input and output species in a reconstructed network. The pathways can be constructed iteratively in order of increasing complexity. SPABBATS allows the accumulation of intermediates in the pathways, which permits discovering pathways missed by most traditional pathway analysis methods. In addition, we provide a proof of concept experiment for the validity of the algorithm. We deleted the genes for the glutamate dehydrogenases of the Gram-positive bacterium Bacillus subtilis and isolated suppressor mutant strains able to grow on glutamate as single carbon source. Our SAT approach proposed candidate alternative pathways which were decisive to pinpoint the exact mutation of the suppressor strain. Conclusions: SPABBATS is the first application of SAT techniques to metabolic problems. It is particularly useful for the characterization of metabolic suppressor mutants and can be used in a synthetic biology setting to design new pathways with specific input-output requirements."],["dc.identifier.doi","10.1186/1752-0509-5-5"],["dc.identifier.isi","000286521700001"],["dc.identifier.pmid","21219666"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7228"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24070"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1752-0509"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","883"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Toedter, Dominik"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Stuelke, Joerg"],["dc.date.accessioned","2018-11-07T10:23:44Z"],["dc.date.available","2018-11-07T10:23:44Z"],["dc.date.issued","2017"],["dc.description.abstract","In most bacteria, fatty acid biosynthesis is an essential process that must be controlled by the availability of precursors and by the needs of cell division. So far, no mechanisms controlling synthesis of malonyl-coenzyme A (CoA), the committed step in fatty acid synthesis, have been identified in the Gram-positive model bacterium Bacillus subtilis. We have studied the localization and function of two highly expressed proteins of unknown function, YqhY and YloU. Both proteins are members of the conserved and widespread Asp23 family. While the deletion of yloU had no effect, loss of the yqhY gene induced the rapid acquisition of suppressor mutations. The vast majority of these mutations affect subunits of the acetyl-CoA carboxylase (ACCase) complex, the enzyme that catalyzes the formation of malonyl-CoA. Moreover, lack of yqhY is accompanied by the formation of lipophilic clusters in the polar regions of the cells indicating an increased activity of ACCase. Our results suggest that YqhY controls the activity of ACCase and that this control results in inhibition of ACCase activity. Hyperactivity of the enzyme complex in the absence of YqhY does then provoke mutations that cause reduced ACCase activity."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [SFB860]"],["dc.identifier.doi","10.3389/fmicb.2017.00883"],["dc.identifier.isi","000401692200001"],["dc.identifier.pmid","28579978"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14498"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42519"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Frontiers Media Sa"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The Highly Conserved Asp23 Family Protein YqhY Plays a Role in Lipid Biosynthesis in Bacillus subtilis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","840846"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Lang, Vanessa"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Ortlepp, Jan Rudolf"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Groß, U."],["dc.date.accessioned","2022-04-04T13:15:05Z"],["dc.date.available","2022-04-04T13:15:05Z"],["dc.date.issued","2022"],["dc.description.abstract","Nosocomial infections with Clostridioides (Clostridium) difficile have become an emergent health threat. We sought to define risk factors for a C. difficile infection (CDI) beyond the widely known ones, such as antibiotic use and prior hospital stay. We therefore focused on a group of patients with diarrhea in order to identify risk factors for C. difficile infection among this symptomatic cohort. A total of 121 hospitalized patients from Seesen/Germany with diarrhea were included who submitted a stool sample and were interviewed about their socio-demographic background, lifestyle and state of health using a standardized questionnaire. Antibiotic potential of diuretics was examined by agar diffusion test. C. difficile was identified in 29 patients resulting in a prevalence of 24.0%. The infection was hospital-acquired in most cases (p < 0.001, 82.1%; n = 23/28, versus 29/91, 31.9%). The generally accepted risk factor previous antibiotic use was confirmed in this study (p = 0.002, n = 23/28 CDI patients, 82.1%, versus n = 44/91 non-CDI patients, 48.4%). The following additional risk factors were identified: regular consumption of proton pump inhibitors; PPI (p = 0.011, n = 24/29, 82.8% vs. n = 52/92, 56.5%), CDI patients ate less vegetables (p = 0.001, n = 12/29, 41.4% vs. 69/92, 75.0%). The intake of the diuretic agent torasemid in patients with CDI (p = 0.005, n = 18/29, 62.1%) was higher than in patients without (n = 30/92, 32.6%). More patients with CDI had to undergo a surgery in the previous year (p = 0.022, n = 13/29, 44.8% vs. n = 21/92, 22.8%) and held more birds (p = 0.056, n = 4/29, 13.8%) than individuals of the negative group (n = 3/92, 3.3%). In conclusion, although no antibiotic potential was detected in diuretics, especially torasemid seems to have significant influence for the occurrence of a CDI as well as a nutrition poor in vegetables. A diet rich in vegetables represented a fourfold lower risk for a CDI (OR 0.240, CI (0.0720 - 0.796])."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.3389/fmicb.2022.840846"],["dc.identifier.pmid","35359708"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106201"],["dc.language.iso","en"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.title","Risk Factors of Patients With Diarrhea for Having Clostridioides (Clostridium) difficile Infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]