Thurow, Corinna

[["dc.bibliographiccitation.firstpage","19897"],["dc.bibliographiccitation.issue","26"],["dc.bibliographiccitation.journal","Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","19905"],["dc.bibliographiccitation.volume","275"],["dc.contributor.author","Niggeweg, R."],["dc.contributor.author","Thurow, C."],["dc.contributor.author","Kegler, C."],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2018-11-07T10:43:38Z"],["dc.date.available","2018-11-07T10:43:38Z"],["dc.date.issued","2000"],["dc.description.abstract","In higher plants, activating sequence-1 (as-1) of the cauliflower mosaic virus 35 S promoter mediates both salicylic acid (SA)- and auxin-inducible transcriptional activation. Originally found in promoters of several viral and bacterial plant pathogens, as-1-like elements are also functional elements of plant promoters activated in the course of a defense response upon pathogen attack. Nuclear as-1-binding factor (ASF-1) and cellular salicylic acid response protein (SARP) bind specifically to as-1. Four different tobacco bZIP transcription factors (TGA1a, PG13, TGA2.1, and TGA2.2) are potential components of either ASF-1 or SARP. Here we show that ASF-1 and SARP are very similar in their composition. TGA2.2 is a major component of either complex, as shown by supershift analysis and Western blot analysis of DNA affinity-purified SARP. Minor amounts of a protein immunologically related to TGA2.1 were detected, whereas TGA1a was not detectable. Overexpression of either TGA2.2 or a dominant negative TGA2.2 mutant affected both SA and auxin (2,4D) inducibility of various target promoters encoding as-1-like elements, albeit to different extents. This indicates that TGA2.2 is a component of the enhancosome assembling on these target promoters, both under elevated SA and 2,4D concentrations. However, the effect of altered TGA2.2 levels on gene expression was more pronounced upon SA treatment than upon 2,4D treatment."],["dc.identifier.doi","10.1074/jbc.M909267199"],["dc.identifier.isi","000087941300064"],["dc.identifier.pmid","10751419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47102"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Biochemistry Molecular Biology Inc"],["dc.relation.issn","0021-9258"],["dc.title","Tobacco transcription factor TGA2.2 is the main component of as-1-binding factor ASF-1 and is involved in salicylic acid- and auxin-inducible expression of as-1-containing target promoters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","823"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","New Phytologist"],["dc.bibliographiccitation.lastpage","837"],["dc.bibliographiccitation.volume","202"],["dc.contributor.author","König, Stefanie"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Gatz, Christiane"],["dc.contributor.author","Polle, Andrea"],["dc.contributor.author","Feußner, Ivo"],["dc.date.accessioned","2017-09-07T11:50:45Z"],["dc.date.available","2017-09-07T11:50:45Z"],["dc.date.issued","2014"],["dc.description.abstract","Summary - Verticillium longisporum is a soil‐borne vascular pathogen causing economic loss in rape. Using the model plant Arabidopsis this study analyzed metabolic changes upon fungal infection in order to identify possible defense strategies of Brassicaceae against this fungus. - Metabolite fingerprinting identified infection‐induced metabolites derived from the phenylpropanoid pathway. Targeted analysis confirmed the accumulation of sinapoyl glucosides, coniferin, syringin and lignans in leaves from early stages of infection on. At later stages, the amounts of amino acids increased. - To test the contribution of the phenylpropanoid pathway, mutants in the pathway were analyzed. The sinapate‐deficient mutant fah1‐2 showed stronger infection symptoms than wild‐type plants, which is most likely due to the lack of sinapoyl esters. Moreover, the coniferin accumulating transgenic plant UGT72E2‐OE was less susceptible. Consistently, sinapoyl glucose, coniferyl alcohol and coniferin inhibited fungal growth and melanization in vitro, whereas sinapyl alcohol and syringin did not. The amount of lignin was not significantly altered supporting the notion that soluble derivatives of the phenylpropanoid pathway contribute to defense. - These data show that soluble phenylpropanoids are important for the defense response of Arabidopsis against V. longisporum and that metabolite fingerprinting is a valuable tool to identify infection‐relevant metabolic markers."],["dc.identifier.doi","10.1111/nph.12709"],["dc.identifier.gro","3147731"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5128"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","0028-646X"],["dc.title","Soluble phenylpropanoids are involved in the defense response of Arabidopsis against Verticillium longisporum"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","817"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Plant Molecular Biology"],["dc.bibliographiccitation.lastpage","829"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Schiermeyer, A."],["dc.contributor.author","Thurow, C."],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2018-11-07T10:39:51Z"],["dc.date.available","2018-11-07T10:39:51Z"],["dc.date.issued","2003"],["dc.description.abstract","TGA factors constitute a family of conserved plant bZIP transcription factors that regulate transcription from as-1-like elements in response to plant signalling molecules salicylic acid ( SA), methyl jasmonate (MJ) and auxin. Based on sequence similarities, two subclasses of TGA factors have been identified before in tobacco: class I factors (TGA1a and PG13) are preferentially expressed in root tip meristems, whereas class II factors ( TGA2.1 and TGA2.2) are found in leaves and in roots. Here we describe a novel member of the tobacco TGA family (TGA10), which defines a distinct subclass of its own. TGA10 mRNA and TGA10 protein were found in roots but not in leaves of mature tobacco plants. TGA10 binds specifically to the as-1 element, interacts with TGA2.2, and activates transcription in yeast. When ectopically expressed in leaves, TGA10 enhanced SA-, auxin- and MJ-inducibility of target gene Nt103, which responds in the same manner to enhanced levels of TGA2.2. This indicates that TGA10, albeit normally not present in leaves, can interact with the leaf regulatory network controlling transcription from as-1-containing promoters. However, Nt103 expression was not affected in roots of TGA10-over-expressing plants, implying the existence of root-specific mechanisms which do not allow a positive effect of increased TGA10 levels on target gene expression."],["dc.identifier.doi","10.1023/A:1023093101976"],["dc.identifier.isi","000181944900002"],["dc.identifier.pmid","12777042"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46155"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Kluwer Academic Publ"],["dc.relation.issn","0167-4412"],["dc.title","Tobacco bZIP factor TGA10 is a novel member of the TGA family of transcription factors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","391"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PLANT PHYSIOLOGY"],["dc.bibliographiccitation.lastpage","402"],["dc.bibliographiccitation.volume","159"],["dc.contributor.author","Koester, Julia"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Kruse, Kerstin"],["dc.contributor.author","Meier, Alexander"],["dc.contributor.author","Iven, Tim"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2018-11-07T09:10:47Z"],["dc.date.available","2018-11-07T09:10:47Z"],["dc.date.issued","2012"],["dc.description.abstract","Plants modify harmful substances through an inducible detoxification system. In Arabidopsis (Arabidopsis thaliana), chemical induction of the cytochrome P450 gene CYP81D11 and other genes linked to the detoxification program depends on class II TGA transcription factors. CYP81D11 expression is also induced by the phytohormone jasmonic acid (JA) through the established pathway requiring the JA receptor CORONATINE INSENSITIVE1 (COI1) and the JA-regulated transcription factor MYC2. Here, we report that the xenobiotic-and the JA-dependent signal cascades have become interdependent at the CYP81D11 promoter. On the one hand, MYC2 can only activate the expression of CYP81D11 when both the MYC2- and the TGA-binding sites are present in the promoter. On the other hand, the xenobiotic-regulated class II TGA transcription factors can only mediate maximal promoter activity if TGA and MYC2 binding motifs, MYC2, and the JA-isoleucine biosynthesis enzymes DDE2/AOS and JAR1 are functional. Since JA levels and degradation of JAZ1, a repressor of the JA response, are not affected by reactive chemicals, we hypothesize that basal JA signaling amplifies the response to chemical stress. Remarkably, stress-induced expression levels were 3-fold lower in coi1 than in the JA biosynthesis mutant dede2-2, revealing that COI1 can contribute to the activation of the promoter in the absence of JA. Moreover, we show that deletion of the MYC2 binding motifs abolishes the JA responsiveness of the promoter but not the responsiveness to COI1. These findings suggest that yet unknown cis-element(s) can mediate COI1-dependent transcriptional activation in the absence of JA."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [GA330/20-1]"],["dc.identifier.doi","10.1104/pp.112.194274"],["dc.identifier.isi","303657100031"],["dc.identifier.pmid","22452854"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26570"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Plant Biologists"],["dc.relation.issn","0032-0889"],["dc.title","Xenobiotic- and Jasmonic Acid-Inducible Signal Transduction Pathways Have Become Interdependent at the Arabidopsis CYP81D11 Promoter"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","45"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Protein Engineering Design and Selection"],["dc.bibliographiccitation.lastpage","52"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Islam, Kazi Mohammed Didarul"],["dc.contributor.author","Dilcher, Meik"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Vock, Carsten A."],["dc.contributor.author","Krimmelbein, Ilga Kristine"],["dc.contributor.author","Tietze, Lutz Friedjan"],["dc.contributor.author","Gonzalez, Victor"],["dc.contributor.author","Zhao, Huimin"],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2018-11-07T08:35:26Z"],["dc.date.available","2018-11-07T08:35:26Z"],["dc.date.issued","2009"],["dc.description.abstract","Transcriptional activators that respond to ligands with no cellular targets are powerful tools that can confer regulated expression of a transgene in almost all biological systems. In this study, we altered the ligand-binding specificity of the human estrogen receptor alpha (hER alpha) so that it would recognize a non-steroidal synthetic compound with structural similarities to the phytoestrogen resveratrol. For this purpose, we performed iterative rounds of site-specific saturation mutagenesis of a fixed set of ligand-contacting residues and subsequent random mutagenesis of the entire ligand-binding domain. Selection of the receptor mutants and quantification of the interaction were carried out by exploiting a yeast two-hybrid system that reports the ligand-dependent interaction between hER alpha and steroid receptor coactivator-1 (SRC-1). The screen was performed with a synthetic ligand (CV3320) that promoted growth of the reporter yeast strain to half maximal levels at a concentration of 3.7 mu M. The optimized receptor mutant (L384F/L387M/Y537S) showed a 67-fold increased activity to the synthetic ligand CV3320 (half maximal yeast growth at 0.055 mu M) and a 10-fold decreased activity to 17 ss-estradiol (E2; half maximal yeast growth at 4 nM). The novel receptor-ligand pair partially fulfills the requirements for a specific 'gene switch' as it responds to concentrations of the synthetic ligand which do not activate the wildtype receptor. Due to its residual responsiveness to E2 at concentrations (4 nM) that might occur in vivo, further improvements have to be performed to render the system applicable in organisms with endogenous E2 synthesis."],["dc.identifier.doi","10.1093/protein/gzn067"],["dc.identifier.isi","000261908700006"],["dc.identifier.pmid","19088113"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18068"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1741-0126"],["dc.title","Directed evolution of estrogen receptor proteins with altered ligand-binding specificities"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1906"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","The New Phytologist"],["dc.bibliographiccitation.lastpage","1918"],["dc.bibliographiccitation.volume","221"],["dc.contributor.author","Li, Ning"],["dc.contributor.author","Muthreich, Martin"],["dc.contributor.author","Huang, Li‐Jun"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Sun, Tongjun"],["dc.contributor.author","Zhang, Yuelin"],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2020-12-10T18:36:17Z"],["dc.date.available","2020-12-10T18:36:17Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1111/nph.15496"],["dc.identifier.eissn","1469-8137"],["dc.identifier.issn","0028-646X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76570"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","TGACG‐BINDING FACTORs (TGAs) and TGA‐interacting CC‐type glutaredoxins modulate hyponastic growth in Arabidopsis thaliana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","100"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Plant Journal"],["dc.bibliographiccitation.lastpage","113"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Thurow, C."],["dc.contributor.author","Schiermeyer, A."],["dc.contributor.author","Krawczyk, S."],["dc.contributor.author","Butterbrodt, T."],["dc.contributor.author","Nickolov, K."],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2018-11-07T10:55:35Z"],["dc.date.available","2018-11-07T10:55:35Z"],["dc.date.issued","2005"],["dc.description.abstract","Salicylic acid (SA) is a crucial internal signaling molecule needed for the induction of plant defense responses upon attack of a variety of pathogens. Basic leucine zipper transcription factors of the TGA family bind to activating sequence-1 (as-1)-like elements which are SA-responsive cis elements found in promoters of 'immediate early' and 'late' SA-inducible genes. TGA2.2 constitutes the main component of tobacco as-1-binding factor-1 (ASF-1). TGA2.1, which differs from TGA2.2 by being able to activate transcription in yeast, constitutes a minor fraction of the complex. Both proteins interact with NPR1, a protein essential for SA inducibility of 'late' genes. Here we demonstrate using dsRNAi mediated gene silencing that reducing the amount of TGA2.2 and TGA2.1 correlates with a significant decrease in ASF-1 activity and with a decreased inducibility of both 'immediate early' and 'late' genes. In contrast, reducing the amount of TGA2.1 alone had no effect on the expression of these target genes suggesting that TGA2.1 is dispensable for SA-inducible gene expression from the as-1 element. Expression of a TGA2.2 mutant unable to form heterodimers with the endogenous pool of TGA factors led to reduced SA-inducibility of 'immediate early' gene Nt103, indicating that the native leucine zipper is important for the protein to act positively on transcription. Plants with reduced amounts of TGA2.1 developed petal like stamens indicating a regulatory role of TGA2.1 in defining organ identity in tobacco flowers. A model is suggested that unifies conflicting results on the function of tobacco TGA factors with respect to activation of the 'late'PR-1a promoter."],["dc.identifier.doi","10.1111/j.1365-313X.2005.02513.x"],["dc.identifier.isi","000231952000009"],["dc.identifier.pmid","16167899"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49821"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0960-7412"],["dc.title","Tobacco bZIP transcription factor TGA2.2 and related factor TGA2.1 have distinct roles in plant defense responses and plant development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1805"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLANT PHYSIOLOGY"],["dc.bibliographiccitation.lastpage","1818"],["dc.bibliographiccitation.volume","154"],["dc.contributor.author","Pape, Sebastian"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2018-11-07T08:36:30Z"],["dc.date.available","2018-11-07T08:36:30Z"],["dc.date.issued","2010"],["dc.description.abstract","Systemic acquired resistance is a broad-spectrum plant immune response involving massive transcriptional reprogramming. The Arabidopsis (Arabidopsis thaliana) PATHOGENESIS-RELATED-1 (PR-1) gene has been used in numerous studies to elucidate transcriptional control mechanisms regulating systemic acquired resistance. WRKY transcription factors and basic leucine zipper proteins of the TGA family regulate the PR-1 promoter by binding to specific cis-elements. In addition, the promoter is under the control of two proteins that do not directly contact the DNA: the positive regulator NONEXPRESSOR OF PR GENES1 (NPR1), which physically interacts with TGA factors, and the repressor SUPPRESSOR OF NPR1, INDUCIBLE1 (SNI1). In this study, we analyzed the importance of the TGA-binding sites LS5 and LS7 and the WKRY box LS4 for regulation by NPR1 and SNI1. In the absence of LS5 and LS7, NPR1 activates the PR-1 promoter through a mechanism that requires LS4. Since transcriptional activation of WRKY genes is under the control of NPR1 and since LS4 is not sufficient for the activation of a truncated PR-1 promoter by the effector protein NPR1-VP16 in transient assays, it is concluded that the LS4-dependent activation of the PR-1 promoter is indirect. In the case of NPR1 acting directly through TGA factors at its target promoters, two TGA-binding sites are necessary but not sufficient for NPR1 function in transgenic plants and in the NPR-VP16-based transactivation assay in protoplasts. SNI1 exerts its negative effect in the noninduced state by targeting unknown proteins associated with sequences between bp -816 and -573. Under induced conditions, SNI1 negatively regulates the function of WRKY transcription factors binding to WKRY boxes between bp -550 and -510."],["dc.identifier.doi","10.1104/pp.110.165563"],["dc.identifier.isi","000284834000018"],["dc.identifier.pmid","20935179"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18328"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Plant Biologists"],["dc.relation.issn","0032-0889"],["dc.title","The Arabidopsis PR-1 Promoter Contains Multiple Integration Sites for the Coactivator NPR1 and the Repressor SNI"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","775"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Plant Molecular Biology"],["dc.bibliographiccitation.lastpage","788"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Niggeweg, R."],["dc.contributor.author","Thurow, C."],["dc.contributor.author","Weigel, Ralf"],["dc.contributor.author","Pfitzner, U."],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2018-11-07T09:43:14Z"],["dc.date.available","2018-11-07T09:43:14Z"],["dc.date.issued","2000"],["dc.description.abstract","In higher plants, as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Originally found in viral and T-DNA promoters, they are also functional elements of plant promoters activated during the defence response against pathogens. Tobacco bZIP transcription factor TGA1a was the first recombinant protein shown to bind to as-1. cDNAs for two novel tobacco as-1-binding bZIP proteins (TGA2.1 and TGA2.2) were isolated. Revealing a high degree of amino acid identity in the bZIP domain (89%) and the C-terminus (79%), the two TGA2 factors differ remarkably with respect to the length of the N-terminus (170 amino acids in TGA2.1 versus 43 amino acids in TGA2.2). TGA2.1 and TGA2.2, but not TGA1a, interacted with ankyrin repeat protein NPR1, a central activator of the plant defence response. In contrast, TGA2.1 and TGA1a, but not TGA2.2, functioned as transcriptional activators in yeast. Apart from conferring transcriptional activation, the N-terminal domain of TGA2.1 led to reduced in vitro as-1-binding activity and almost completely abolished binding to one half site of this bifunctional element. When being part of a heterodimer with TGA2.2, TGA2.1 was efficiently recruited to a single half site, though double occupancy of the element was still preferred. In contrast, TGA1a preferred to bind to only one palindrome, a feature that was also maintained in heterodimers between TGA1a and TGA2.1 or TGA2.2."],["dc.identifier.doi","10.1023/A:1006319113205"],["dc.identifier.isi","000086411400010"],["dc.identifier.pmid","10809449"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34135"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","0167-4412"],["dc.title","Tobacco TGA factors differ with respect to interaction with NPR1, activation potential and DNA-binding properties"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","949"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The Plant Journal"],["dc.bibliographiccitation.lastpage","957"],["dc.bibliographiccitation.volume","65"],["dc.contributor.author","Mosblech, Alina"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Gatz, Christiane"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Heilmann, Ingo"],["dc.date.accessioned","2018-11-07T08:59:06Z"],["dc.date.available","2018-11-07T08:59:06Z"],["dc.date.issued","2011"],["dc.description.abstract","Plant responses to wounding are part of their defense responses against insects, and are tightly regulated. The isoleucin conjugate of jasmonic acid (JA-IIe) is a major regulatory molecule. We have previously shown that inositol polyphosphate signals are required for defense responses in Arabidopsis; however, the way in which inositol polyphosphates contribute to plant responses to wounding has so far remained unclear. Arabidopsis F-box proteins involved in the perception of JA-IIe (COI1) and auxin (TIR1) are structurally similar. Because TIR1 has recently been shown to contain inositol hexakisphosphate (InsP(6)) as a co-factor of unknown function, here we explored the possibility that InsP(6) or another inositol polyphosphate is required for COI1 function. In support of this hypothesis, COI1 variants with changes in putative inositol polyphosphate coordinating residues exhibited a reduced interaction with the COI1 target, JAZ9, in yeast two-hybrid tests. The equivalent COI1 variants displayed a reduced capability to rescue jasmonate-mediated root growth inhibition or silique development in Arabidopsis col1 mutants. Yeast two-hybrid tests using wild-type COI1 in an ipk1 Delta yeast strain exhibiting increased levels of inositol pentakisphosphate (InsP(5)) and reduced levels of InsP(6) indicate an enhanced COI1/JAZ9 interaction. Consistent with these findings, Arabidopsis ipk1-1 mutants, also with increased InsP(5) and reduced InsP(6) levels, showed increased defensive capabilities via COI1-mediated processes, including wound-induced gene expression, defense against caterpillars or root growth inhibition by jasmonate. The combined data from experiments using mutated COI1 variants, as well as yeast and Arabidopsis backgrounds altered in inositol polyphosphate metabolism, indicate that an inositol polyphosphate, and probably InsP(5), contributes to COI1 function."],["dc.identifier.doi","10.1111/j.1365-313X.2011.04480.x"],["dc.identifier.isi","000288449700009"],["dc.identifier.pmid","21205029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23810"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0960-7412"],["dc.title","Jasmonic acid perception by COI1 involves inositol polyphosphates in Arabidopsis thaliana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]