Thurow, Corinna

[["dc.bibliographiccitation.firstpage","775"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","NUCLEIC ACIDS RESEARCH"],["dc.bibliographiccitation.lastpage","781"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Krawczyk, Stefanie"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Niggeweg, Ricarda"],["dc.contributor.author","Gatz, Christiane"],["dc.date.accessioned","2019-07-10T08:12:50Z"],["dc.date.available","2019-07-10T08:12:50Z"],["dc.date.issued","2002"],["dc.description.abstract","In higher plants, activation sequence-1 (as-1) of the cauliflower mosaic virus 35S promoter mediates both salicylic acid- and auxin-inducible transcriptional activation. Originally found in viral and T-DNA promoters, as-1-like elements are also functional elements of plant promoters activated in the course of a defence response upon pathogen attack. as-1-like elements are characterised by two imperfect palindromes with the palindromic centres being spaced by 12 bp. They are recognised by plant nuclear as-1-binding factor ASF-1, the major component of which is basic/leucine zipper (bZIP) protein TGA2.2 (~80%) in Nicotiana tabacum. In electrophoretic mobility shift assays, ASF-1 as well as bZIP proteins TGA2.2, TGA2.1 and TGA1a showed a 3–10-fold reduced binding affinity to mutant as-1 elements encoding insertions of 2, 4, 6, 8 or 10 bp between the palindromes, respectively. This correlated with a 5–10-fold reduction in transcriptional activation from these elements in transient expression assays. Although ASF-1 and TGA factors bound efficiently to a mutant element carrying a 2 bp deletion between the palindromes [as-1/(–2)], the latter was strongly compromised with respect to mediating gene expression in vivo. A fusion protein consisting of TGA2.2 and a constitutive activation domain mediated transactivation from as-1/(–2) demonstrating binding of TGA factors in vivo. We therefore conclude that both DNA binding and transactivation require optimal positioning of TGA factors on the as-1 element."],["dc.identifier.fs","28444"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4102"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61055"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0305-1048"],["dc.relation.issn","1362-4962"],["dc.relation.orgunit","Fakultät für Biologie und Psychologie"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Analysis of the spacing between the two palindromes of activation sequence-1 with respect to binding to different TGA factors and transcriptional activation potential."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]