Menger, Björn

[["dc.bibliographiccitation.firstpage","118"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European Journal of Applied Physiology"],["dc.bibliographiccitation.lastpage","130"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Saul, D."],["dc.contributor.author","Ninkovic, M."],["dc.contributor.author","Komrakova, M."],["dc.contributor.author","Wolff, P."],["dc.contributor.author","Simka, P."],["dc.contributor.author","Gasimov, T."],["dc.contributor.author","Menger, B."],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Rohde, V."],["dc.contributor.author","Sehmisch, S."],["dc.date.accessioned","2020-06-15T06:51:43Z"],["dc.date.available","2020-06-15T06:51:43Z"],["dc.date.issued","2017"],["dc.description.abstract","Estrogen deficiency and aging are associated with osteoporosis, impaired bone healing, and lower cognitive performance. Close functional and physical connections occur between bone and the central nervous system. An anti-inflammatory drug, zileuton (which is an inhibitor of arachidonate 5-lipoxygenase), is known to have a positive effect on bone tissue repair and brain ischemia. We studied the effect of zileuton on osteopenic bone and its healing and on the genes considered to be crucial for the cross talks between bone and brain. Three-month-old Sprague-Dawley rats were ovariectomized or left untreated. After 8 wk, bilateral metaphyseal tibia osteotomy with plate osteosynthesis was performed in all rats. Ovariectomized rats were fed with food containing zileuton (1, 10, or 100 mg/kg body wt) for 5 wk. In tibiae, bone volume, callus and cortical volume, and gene expression of osteocalcin and alkaline phosphatase were enhanced by zileuton (10 or 100 mg); biomechanical properties and bone density were not changed. In femur, zileuton enlarged cortical volume distal and trabecular volume proximal, decreasing their density. The expression level of brain Sema3a, known to regulate bone mass positively, was downregulated after ovariectomy. In contrast, bone Sema4d, a negative regulator of bone mass, was upregulated in the tibia callus after ovariectomy, whereas zileuton treatment (10 or 100 mg) resulted in reverse effects. Here, we describe for the first time the expression of Rbbp4 mRNA and its increase in tibia after ovariectomy. Zileuton caused downregulation of Rbbp4 in the hippocampus and had an effect on bone healing, changed the expression of genes involved in cross talk between bones and brain, and may be a potent drug for further examination in estrogen deficiency-related dysfunction(s)."],["dc.identifier.doi","10.1152/japplphysiol.01126.2016"],["dc.identifier.pmid","28860177"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66241"],["dc.language.iso","en"],["dc.relation.eissn","1522-1601"],["dc.relation.issn","8750-7587"],["dc.title","Effect of zileuton on osteoporotic bone and its healing, expression of bone and brain genes in rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","134"],["dc.bibliographiccitation.journal","Bone"],["dc.bibliographiccitation.lastpage","144"],["dc.bibliographiccitation.volume","101"],["dc.contributor.author","Saul, D."],["dc.contributor.author","Gleitz, S."],["dc.contributor.author","Nguyen, H. H."],["dc.contributor.author","Kosinsky, R. L."],["dc.contributor.author","Sehmisch, S."],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Wassmann, M."],["dc.contributor.author","Menger, B."],["dc.contributor.author","Komrakova, M."],["dc.date.accessioned","2018-11-07T10:21:53Z"],["dc.date.accessioned","2020-06-15T07:16:22Z"],["dc.date.available","2018-11-07T10:21:53Z"],["dc.date.available","2020-06-15T07:16:22Z"],["dc.date.issued","2017"],["dc.description.abstract","Osteoporosis is one of the most common diseases worldwide. In osteoporosis, vertebral fractures represent a major burden. Lipoxygenase (LOX) inhibitors such as baicalein and zileuton may represent a promising therapeutic option owing to their antioxidative effects and suppression of various inflammatory processes in muscle and bone. The effect of these LOX inhibitors on the spine was studied in osteopenic rats. Female Sprague-Dawley rats were divided two times into five groups: four groups each were ovariectomized (OVX) and one control group was non-ovariectomized (NON-OVX). Eight weeks after ovariectomy, three concentrations of baicalein (1 mg/kg body weight [BW], 10 mg/kg BW, and 100 mg/kg BW) were administered subcutaneously daily in three OVX groups for 4 weeks. Similarly, zileuton was administered in three concentrations via food for 5 weeks. In vivo computed tomography (pQCT) of the spine was performed before the treatments and at the end of the experiment. Lumbar vertebrae were subjected to a compression test, micro-CT, and ashing analyses. After baicalein treatment, cortical bone mineral density (BMD) was improved; trabecular connectivity and trabecular BMD were diminished at high dose. After zileuton treatment the total BMD, anorganic weight, trabecular nodes, and trabecular area were improved. The in vivo stress-strain index was increased and alkaline phosphatase activity in serum was enhanced after both treatments. A dose-dependent effect was not clearly observed after both treatments. The treatments using baicalein for 4 and zileuton for 5 weeks were not sufficient to change the biomechanical properties and bone volume fraction (BV/TV). Overall, baicalein improved the cortical bone parameters whereas zileuton had a favorable effect on the trabecular structure. Moreover, both treatments increased the bone formation rate. Longer trials, a combination of both LOX inhibitors, and their effect at the cellular and molecular levels should be investigated in further studies. (C) 2017 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","Elsbeth Bonhoff Stiftung [N114]"],["dc.identifier.doi","10.1016/j.bone.2017.04.011"],["dc.identifier.isi","000404319300017"],["dc.identifier.pmid","28455215"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42182"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","8756-3282"],["dc.relation.issn","1873-2763"],["dc.title","Effect of the lipoxygenase-inhibitors baicalein and zileuton on the vertebra in ovariectomized rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]