Slotta, Jan Erik

[["dc.bibliographiccitation.firstpage","713"],["dc.bibliographiccitation.journal","American Journal of Transplantation"],["dc.bibliographiccitation.lastpage","714"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Schmitz, J."],["dc.contributor.author","Beck, J."],["dc.contributor.author","Slotta, Jan Erik"],["dc.contributor.author","Walson, Philip D."],["dc.date.accessioned","2018-11-07T09:39:13Z"],["dc.date.available","2018-11-07T09:39:13Z"],["dc.date.issued","2014"],["dc.identifier.isi","000338033303192"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33230"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","World Transplant Congress"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","1600-6143"],["dc.relation.issn","1600-6135"],["dc.title","Potential of Graft-Derived Cell-Free DNA Quantification to Improve Outcomes With Marginal Donor Organs - Case Report of Successful Liver Transplantation of a HELLP Syndrome Donor."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S241"],["dc.bibliographiccitation.journal","Liver Transplantation"],["dc.bibliographiccitation.lastpage","S242"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Slotta, Jan Erik"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Schuetz, Ekkehardt"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Kollmar, Otto"],["dc.date.accessioned","2018-11-07T09:39:07Z"],["dc.date.available","2018-11-07T09:39:07Z"],["dc.date.issued","2014"],["dc.identifier.isi","000339959602142"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33206"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","Joint International Congress of ILTS, ELITA and LICAGE"],["dc.relation.eventlocation","London, ENGLAND"],["dc.relation.issn","1527-6473"],["dc.relation.issn","1527-6465"],["dc.title","Graft Derived Cell-Free DNA as an Early Organ Integrity Biomarker after Liver Transplantation of a HELLP Syndrome Donor Liver."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1732"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Clinical Chemistry"],["dc.bibliographiccitation.lastpage","1741"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Bierau, Sarah"],["dc.contributor.author","Balzer, Stefan"],["dc.contributor.author","Andag, Reiner"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Gaedcke, Jochen"],["dc.contributor.author","Mörer, Onnen"],["dc.contributor.author","Slotta, Jan E."],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Schütz, Ekkehard"],["dc.date.accessioned","2020-05-22T07:07:40Z"],["dc.date.available","2020-05-22T07:07:40Z"],["dc.date.issued","2013"],["dc.description.abstract","BACKGROUND Cell-free DNA (cfDNA) from grafts in the circulation of transplant recipients is a potential biomarker of rejection. Its usefulness was investigated after heart transplantation during the maintenance phase by use of microarrays and massive parallel sequencing of donor and recipient DNA. Disadvantages of these methods are high costs, long turnaround times, and need for donor DNA. Therefore, we sought to develop a rapid and cost-effective method using digital droplet PCR (ddPCR). METHODS Plasma samples were collected from stable recipients after liver (LTx, n = 10), kidney (KTx, n = 9), and heart (HTx, n = 8) transplantation as well as from 7 additional patients directly after LTx. Known single-nucleotide polymorphisms were selected for high minor allelic frequencies, of which 41 hydrolysis probe assays were established. Plasma cfDNA was preamplified, followed by conventional real-time PCR to define informative (heterologous) SNPs, which were then used for quantification (percentage) of graft-derived cfDNA (GcfDNA) using ddPCR. RESULTS Mean recovery was 94% (SD, 13%) with an imprecision of 4%–14% with the use of controls with 2% minor allele. GcfDNA in stable patients was <6.8% (LTx), <2.5% (KTx), and <3.4% (HTx). On the day of LTx, GcfDNA was approximately 90% and by day 10 it was <15% in complication-free LTx recipients. In 2 patients with biopsy-proven rejection, GcfDNA increased to >60%, whereas in 1 patient with cholestasis no increase was found. CONCLUSIONS A novel, cost-effective, rapid technique was developed to quantify GcfDNA in transplant recipients. This technique embodies a promising, potentially universal biomarker for early detection of rejection, which could enable more effective therapeutic interventions."],["dc.identifier.doi","10.1373/clinchem.2013.210328"],["dc.identifier.pmid","24061615"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65680"],["dc.language.iso","en"],["dc.relation.eissn","1530-8561"],["dc.relation.issn","0009-9147"],["dc.title","Digital droplet PCR for rapid quantification of donor DNA in the circulation of transplant recipients as a potential universal biomarker of graft injury"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Transplant International"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Beck, J."],["dc.contributor.author","Schmitz, J."],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Slotta, Jan Erik"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Schütz, Ekkehard"],["dc.date.accessioned","2018-11-07T09:34:43Z"],["dc.date.available","2018-11-07T09:34:43Z"],["dc.date.issued","2014"],["dc.format.extent","50"],["dc.identifier.isi","000342752400154"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32234"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","1432-2277"],["dc.relation.issn","0934-0874"],["dc.title","USE OF PLASMA GCFDNA QUANTIFICATION TO GUIDE PERSONALIZED IMMUNOSUPPRESSION"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S107"],["dc.bibliographiccitation.journal","Liver Transplantation"],["dc.bibliographiccitation.lastpage","S108"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Slotta, Jan Erik"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Birau, Sarah"],["dc.contributor.author","Balzer, Stefan"],["dc.contributor.author","Andag, Reiner"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Gaedcke, Jochen"],["dc.contributor.author","Moerer, Onnen"],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Schuetz, Eckehardt"],["dc.contributor.author","Kollmar, Otto"],["dc.date.accessioned","2018-11-07T09:39:07Z"],["dc.date.available","2018-11-07T09:39:07Z"],["dc.date.issued","2014"],["dc.identifier.isi","000339959601015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33207"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","Joint International Congress of ILTS, ELITA and LICAGE"],["dc.relation.eventlocation","London, ENGLAND"],["dc.relation.issn","1527-6473"],["dc.relation.issn","1527-6465"],["dc.title","Quantification of Donor DNA in the Circulation after Liver Transplantation As a Potential Universal Rejection Biomarker Using Digital Droplet PCR."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e43"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Transplantation Journal"],["dc.bibliographiccitation.lastpage","e45"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Slotta, Jan Erik"],["dc.date.accessioned","2018-11-07T09:35:15Z"],["dc.date.accessioned","2020-05-22T07:54:43Z"],["dc.date.available","2018-11-07T09:35:15Z"],["dc.date.available","2020-05-22T07:54:43Z"],["dc.date.issued","2014"],["dc.identifier.isi","000341854800007"],["dc.identifier.pmid","25171533"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65700"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.eissn","1534-6080"],["dc.relation.issn","0041-1337"],["dc.title","Graft-Derived Cell-Free DNA as an Early Organ Integrity Biomarker After Transplantation of a Marginal HELLP Syndrome Donor Liver"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","713"],["dc.bibliographiccitation.journal","Transplantation Journal"],["dc.bibliographiccitation.lastpage","714"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Schmitz, J."],["dc.contributor.author","Beck, J."],["dc.contributor.author","Slotta, Jan Erik"],["dc.contributor.author","Walson, Philip D."],["dc.date.accessioned","2018-11-07T09:37:41Z"],["dc.date.available","2018-11-07T09:37:41Z"],["dc.date.issued","2014"],["dc.identifier.isi","000339104604346"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32897"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","World Transplant Congress"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","1534-6080"],["dc.relation.issn","0041-1337"],["dc.title","Potential of Graft-Derived Cell-Free DNA Quantification to Improve Outcomes With Marginal Donor Organs - Case Report of Successful Liver Transplantation of a HELLP Syndrome Donor."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]