Kruss, Sebastian

[["dc.bibliographiccitation.artnumber","10241"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Bisker, Gili"],["dc.contributor.author","Dong, Juyao"],["dc.contributor.author","Park, Hoyoung D."],["dc.contributor.author","Iverson, Nicole M."],["dc.contributor.author","Ahn, Jiyoung"],["dc.contributor.author","Nelson, Justin T."],["dc.contributor.author","Landry, Markita P."],["dc.contributor.author","Kruss, Sebastian"],["dc.contributor.author","Strano, Michael S."],["dc.date.accessioned","2018-11-07T10:21:13Z"],["dc.date.available","2018-11-07T10:21:13Z"],["dc.date.issued","2016"],["dc.description.abstract","Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by 480% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications."],["dc.identifier.doi","10.1038/ncomms10241"],["dc.identifier.isi","000369018800004"],["dc.identifier.pmid","26742890"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12894"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42048"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","2041-1723"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.title","Protein-targeted corona phase molecular recognition"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9104-9115"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Telele, Saba"],["dc.contributor.author","Zelená, Anna"],["dc.contributor.author","Gillen, Alice J."],["dc.contributor.author","Antonucci, Alessandra"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Mann, Florian A."],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Boghossian, Ardemis A."],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-06-26T11:13:39Z"],["dc.date.available","2020-06-26T11:13:39Z"],["dc.date.issued","2020"],["dc.description.abstract","Cells can take up nanoscale materials, which has important implications for understanding cellular functions, biocompatibility as well as biomedical applications. Controlled uptake, transport and triggered release of nanoscale cargo is one of the great challenges in biomedical applications of nanomaterials. Here, we study how human immune cells (neutrophilic granulocytes, neutrophils) take up nanomaterials and program them to release this cargo after a certain time period. For this purpose, we let neutrophils phagocytose DNA-functionalized single-walled carbon nanotubes (SWCNTs) in vitro that fluoresce in the near infrared (980 nm) and serve as sensors for small molecules. Cells still migrate, follow chemical gradients and respond to inflammatory signals after uptake of the cargo. To program release, we make use of neutrophil extracellular trap formation (NETosis), a novel cell death mechanism that leads to chromatin swelling, subsequent rupture of the cellular membrane and release of the cell's whole content. By using the process of NETosis, we can program the time point of cargo release via the initial concentration of stimuli such as phorbol 12-myristate-13-acetate (PMA) or lipopolysaccharide (LPS). At intermediate stimulation, cells continue to migrate, follow gradients and surface cues for around 30 minutes and up to several hundred micrometers until they stop and release the SWCNTs. The transported and released SWCNT sensors are still functional as shown by subsequent detection of the neurotransmitter dopamine and reactive oxygen species (H2O2). In summary, we hijack a biological process (NETosis) and demonstrate how neutrophils transport and release functional nanomaterials."],["dc.identifier.doi","10.1039/d0nr00864h"],["dc.identifier.pmid","32286598"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66755"],["dc.language.iso","en"],["dc.relation.eissn","2040-3372"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","CC BY 3.0"],["dc.subject.gro","cellular biophysics"],["dc.title","Transport and programmed release of nanoscale cargo from cells by using NETosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Biophotonics"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Spreinat, Alexander"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2019-12-05T14:29:38Z"],["dc.date.accessioned","2021-10-27T13:12:48Z"],["dc.date.available","2019-12-05T14:29:38Z"],["dc.date.available","2021-10-27T13:12:48Z"],["dc.date.issued","2019"],["dc.description.abstract","Multispectral imaging combines the spectral resolution of spectroscopy with the spatial resolution of imaging and is therefore very useful for biomedical applications. Currently, histological diagnostics use mainly stainings with standard dyes (eg, hematoxylin + eosin) to identify tumors. This method is not applicable in vivo and provides low amounts of chemical information. Biomolecules absorb near infrared light (NIR, 800-1700 nm) at different wavelengths, which could be used to fingerprint tissue. Here, we built a NIR multispectral absorption imaging setup to study skin tissue samples. NIR light (900-1500 nm) was used for homogenous wide-field transmission illumination and detected by a cooled InGaAs camera. In this setup, images I(x, y, λ) from dermatological samples (melanoma, nodular basal-cell carcinoma, squamous-cell carcinoma) were acquired to distinguish healthy from diseased tissue regions. In summary, we show the potential of multispectral NIR imaging for cancer diagnostics."],["dc.description.sponsorship","life@nano"],["dc.identifier.doi","10.1002/jbio.201960080"],["dc.identifier.eissn","1864-0648"],["dc.identifier.isbn","31602799"],["dc.identifier.issn","1864-063X"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16850"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91723"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.publisher","WILEY‐VCH Verlag GmbH \\u0026 Co. KGaA"],["dc.relation.eissn","1864-0648"],["dc.relation.issn","1864-0648"],["dc.relation.issn","1864-063X"],["dc.relation.orgunit","Fakultät für Chemie"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject","diagnostics; histology; multispectral imaging; near infrared spectroscopy; skin cancer"],["dc.subject.ddc","540"],["dc.title","Multispectral near infrared absorption imaging for histology of skin cancer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12"],["dc.bibliographiccitation.journal","Frontiers in Immunology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.affiliation","Neubert, Elsa; 1Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Senger-Sander, Susanne N.; 1Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Manzke, Veit S.; 1Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Busse, Julia; 1Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Polo, Elena; 2Institute of Physical Chemistry, University of Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Scheidmann, Sophie E. F.; 1Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Schön, Michael P.; 1Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Kruss, Sebastian; 2Institute of Physical Chemistry, University of Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Erpenbeck, Luise; 1Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Senger-Sander, Susanne N."],["dc.contributor.author","Manzke, Veit S."],["dc.contributor.author","Busse, Julia"],["dc.contributor.author","Polo, Elena"],["dc.contributor.author","Scheidmann, Sophie E. F."],["dc.contributor.author","Schön, Michael P."],["dc.contributor.author","Kruss, Sebastian"],["dc.contributor.author","Erpenbeck, Luise"],["dc.date.accessioned","2019-07-09T11:50:03Z"],["dc.date.available","2019-07-09T11:50:03Z"],["dc.date.issued","2019"],["dc.date.updated","2022-02-09T13:23:16Z"],["dc.description.abstract","The formation of neutrophil extracellular traps (NETs) is an immune defense mechanism of neutrophilic granulocytes. Moreover, it is also involved in the pathogenesis of autoimmune, inflammatory, and neoplastic diseases. For that reason, the process of NET formation (NETosis) is subject of intense ongoing research. In vitro approaches to quantify NET formation are commonly used and involve neutrophil stimulation with various activators such as phorbol 12-myristate 13-acetate (PMA), lipopolysaccharides (LPS), or calcium ionophores (CaI). However, the experimental conditions of these experiments, particularly the media and media supplements employed by different research groups, vary considerably, rendering comparisons of results difficult. Here, we present the first standardized investigation of the influence of different media supplements on NET formation in vitro. The addition of heat-inactivated (hi) fetal calf serum (FCS), 0.5% human serum albumin (HSA), or 0.5% bovine serum albumin (BSA) efficiently prevented NET formation of human neutrophils following stimulation with LPS and CaI, but not after stimulation with PMA. Thus, serum components such as HSA, BSA and hiFCS (at concentrations typically found in the literature) inhibit NET formation to different degrees, depending on the NETosis inducer used. In contrast, in murine neutrophils, NETosis was inhibited by FCS and BSA, regardless of the inducer employed. This shows that mouse and human neutrophils have different susceptibilities toward the inhibition of NETosis by albumin or serum components. Furthermore, we provide experimental evidence that albumin inhibits NETosis by scavenging activators such as LPS. We also put our results into the context of media supplements most commonly used in NET research. In experiments with human neutrophils, either FCS (0.5-10%), heat-inactivated (hiFCS, 0.1-10%) or human serum albumin (HSA, 0.05-2%) was commonly added to the medium. For murine neutrophils, serum-free medium was used in most cases for stimulation with LPS and CaI, reflecting the different sensitivities of human and murine neutrophils to media supplements. Thus, the choice of media supplements greatly determines the outcome of experiments on NET-formation, which must be taken into account in NETosis research."],["dc.identifier.doi","10.3389/fimmu.2019.00012"],["dc.identifier.eissn","1664-3224"],["dc.identifier.pmid","30733715"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15847"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59691"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-3224"],["dc.relation.issn","1664-3224"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Serum and Serum Albumin Inhibit in vitro Formation of Neutrophil Extracellular Traps (NETs)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Dohmen, Maria"],["dc.contributor.author","Walter, Sebastian G."],["dc.contributor.author","Noll, Christine"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2021-04-14T08:27:19Z"],["dc.date.available","2021-04-14T08:27:19Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1038/s41467-020-19718-5"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17801"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82239"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Remote near infrared identification of pathogens with multiplexed nanosensors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4541"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Nanoscale Advances"],["dc.bibliographiccitation.lastpage","4553"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Weitzel, Milan"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Oswald, Tabea A."],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Karius, Volker"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2021-08-12T07:45:03Z"],["dc.date.available","2021-08-12T07:45:03Z"],["dc.date.issued","2021"],["dc.description.abstract","The layered silicates Egyptian Blue (CaCuSi 4 O 10 , EB), Han Blue (BaCuSi 4 O 10 , HB) and Han Purple (BaCuSi 2 O 6 , HP) emit as bulk materials bright and stable fluorescence in the near-infrared (NIR), which is of high interest for (bio)photonics due to minimal scattering, absorption and phototoxicity in this spectral range. So far the optical properties of nanosheets (NS) of these silicates are poorly understood. Here, we exfoliate them into monodisperse nanosheets, report their physicochemical properties and use them for (bio)photonics. The approach uses ball milling followed by tip sonication and centrifugation steps to exfoliate the silicates into NS with lateral size and thickness down to ≈ 16–27 nm and 1–4 nm, respectively. They emit at ≈ 927 nm (EB-NS), 953 nm (HB-NS) and 924 nm (HP-NS), and single NS can be imaged in the NIR. The fluorescence lifetimes decrease from ≈ 30–100 μs (bulk) to 17 μs (EB-NS), 8 μs (HB-NS) and 7 μs (HP-NS), thus enabling lifetime-encoded multicolor imaging both on the microscopic and the macroscopic scale. Finally, remote imaging through tissue phantoms reveals the potential for bioimaging. In summary, we report a procedure to gain monodisperse NIR fluorescent silicate nanosheets, determine their size-dependent photophysical properties and showcase the potential for NIR photonics."],["dc.identifier.doi","10.1039/D1NA00238D"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88360"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/324"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2516-0230"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e2202842119"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.volume","119"],["dc.contributor.author","Elizarova, Sofia"],["dc.contributor.author","Chouaib, Abed Alrahman"],["dc.contributor.author","Shaib, Ali"],["dc.contributor.author","Hill, Björn"],["dc.contributor.author","Mann, Florian"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Kruss, Sebastian"],["dc.contributor.author","Daniel, James A."],["dc.date.accessioned","2022-06-01T09:39:16Z"],["dc.date.available","2022-06-01T09:39:16Z"],["dc.date.issued","2022"],["dc.description.abstract","Significance The neurotransmitter dopamine controls normal behavior and dopaminergic dysfunction is prevalent in multiple brain diseases. To reach a detailed understanding of how dopamine release and signaling are regulated at the subcellular level, we developed a near infrared fluorescent dopamine nanosensor 'paint' (AndromeDA) to directly image dopamine release and its spatiotemporal characteristics. With AndromeDA, we can ascribe discrete DA release events to defined axonal varicosities, directly assess the heterogeneity of DA release events across such release sites, and determine the molecular components of the DA release machinery. AndromeDA thus provides a new method for gaining fundamental insights into the core mechanisms of dopamine release, which with greatly benefit our knowledge of dopamine biology and pathobiology."],["dc.identifier.doi","10.1073/pnas.2202842119"],["dc.identifier.pmid","35613050"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/108429"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/493"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-572"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.relation.workinggroup","RG Brose"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0/"],["dc.title","A fluorescent nanosensor paint detects dopamine release at axonal varicosities with high spatiotemporal resolution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Chizhik, Alexey"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Kuhlemann, llyas"],["dc.contributor.author","Meyer, Daniel"],["dc.contributor.author","Vuong, Loan"],["dc.contributor.author","Preiß, Helen"],["dc.contributor.author","Herrmann, Niklas"],["dc.contributor.author","Mann, Florian A."],["dc.contributor.author","Lv, Zhiyi"],["dc.contributor.author","Oswald, Tabea A."],["dc.contributor.author","Spreinat, Alexander"],["dc.contributor.author","Erpenbeck, Luise"],["dc.contributor.author","Großhans, Jörg"],["dc.contributor.author","Karius, Volker"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Pablo Giraldo, Juan"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2020-11-05T15:08:10Z"],["dc.date.available","2020-11-05T15:08:10Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.1038/s41467-020-15299-5"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17352"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68478"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-352.7"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","2041-1723"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Exfoliated near infrared fluorescent silicate nanosheets for (bio)photonics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11159"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.lastpage","11166"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Nißler, Robert"],["dc.contributor.author","Mann, Florian A."],["dc.contributor.author","Preiß, Helen"],["dc.contributor.author","Selvaggio, Gabriele"],["dc.contributor.author","Herrmann, Niklas"],["dc.contributor.author","Kruss, Sebastian"],["dc.date.accessioned","2019-07-15T10:05:29Z"],["dc.date.accessioned","2021-10-27T13:12:41Z"],["dc.date.available","2019-07-15T10:05:29Z"],["dc.date.available","2021-10-27T13:12:41Z"],["dc.date.issued","2019"],["dc.description.abstract","Single-walled carbon nanotubes (SWCNTs) have unique photophysical properties and serve as building blocks for biosensors, functional materials and devices. For many applications it is crucial to use chirality-pure SWCNTs, which requires sophisticated processes. Purification procedures such as wrapping by certain polymers, phase separation, density gradient centrifugation or gel chromatography have been developed and yield distinct SWCNT species wrapped by a specific polymer or surfactant. However, many applications require a different organic functionalization (corona) around the SWCNTs instead of the one used for the purification process. Here, we present a novel efficient and straightforward process to gain chirality pure SWCNTs with tunable functionalization. Our approach uses polyfluorene (PFO) polymers to enrich certain chiralities but the polymer is removed again and finally exchanged to any desired organic phase. We demonstrate this concept by dispersing SWCNTs in poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(6,6'-{2,2'-bipyridine})] (PFO-BPy), which is known to preferentially solubilize (6,5)-SWCNTs. Then PFO-BPy is removed and recycled, while letting the SWCNTs adsorb/agglomerate on sodium chloride (NaCl) crystals, which act as a toluene-stable but water-soluble filler material. In the last step these purified SWCNTs are redispersed in different polymers, surfactants and ssDNA. This corona phase exchange purification (CPEP) approach was also extended to other PFO variants to enrich and functionalize (7,5)-SWCNTs. CPEP purified and functionalized SWCNTs display monodisperse nIR spectra, which are important for fundamental studies and applications that rely on spectral changes. We show this advantage for SWCNT-based nIR fluorescent sensors for the neurotransmitter dopamine and red-shifted sp3 defect peaks . In summary, CPEP makes use of PFO polymers for chirality enrichment but provides access to chirality enriched SWCNTs functionalized in any desired polymer, surfactant or biopolymer."],["dc.identifier.doi","10.1039/c9nr03258d"],["dc.identifier.pmid","31149692"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16279"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91713"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","2040-3372"],["dc.relation.issn","2040-3364"],["dc.relation.orgunit","Fakultät für Chemie"],["dc.rights","CC BY 3.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.subject","carbon nanotubes; Chirality; CPEP"],["dc.subject.ddc","540"],["dc.title","Chirality enriched carbon nanotubes with tunable wrapping via corona phase exchange purification (CPEP)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Immunology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Neubert, Elsa"],["dc.contributor.author","Bach, Katharina Marie"],["dc.contributor.author","Busse, Julia"],["dc.contributor.author","Bogeski, Ivan"],["dc.contributor.author","Schön, Michael P."],["dc.contributor.author","Kruss, Sebastian"],["dc.contributor.author","Erpenbeck, Luise"],["dc.date.accessioned","2020-12-10T18:44:25Z"],["dc.date.available","2020-12-10T18:44:25Z"],["dc.date.issued","2019"],["dc.description.abstract","Neutrophil Extracellular Traps (NETs) are produced by neutrophilic granulocytes and consist of decondensed chromatin decorated with antimicrobial peptides. They defend the organism against intruders and are released upon various stimuli including pathogens, mediators of inflammation, or chemical triggers. NET formation is also involved in inflammatory, cardiovascular, malignant diseases, and autoimmune disorders like rheumatoid arthritis, psoriasis, or systemic lupus erythematosus (SLE). In many autoimmune diseases like SLE or dermatomyositis, light of the ultraviolet-visible (UV-VIS) spectrum is well-known to trigger and aggravate disease severity. However, the underlying connection between NET formation, light exposure, and disease exacerbation remains elusive. We studied the effect of UVA (375 nm), blue (470 nm) and green (565 nm) light on NETosis in human neutrophils ex vivo. Our results show a dose- and wavelength-dependent induction of NETosis. Light-induced NETosis depended on the generation of extracellular reactive oxygen species (ROS) induced by riboflavin excitation and its subsequent reaction with tryptophan. The light-induced NETosis required both neutrophil elastase (NE) as well as myeloperoxidase (MPO) activation and induced histone citrullination. These findings suggest that NET formation as a response to light could be the hitherto missing link between elevated susceptibility to NET formation in autoimmune patients and photosensitivity for example in SLE and dermatomyositis patients. This novel connection could provide a clue for a deeper understanding of light-sensitive diseases in general and for the development of new pharmacological strategies to avoid disease exacerbation upon light exposure."],["dc.identifier.doi","10.3389/fimmu.2019.02428"],["dc.identifier.eissn","1664-3224"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16550"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78445"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-3224"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Blue and Long-Wave Ultraviolet Light Induce in vitro Neutrophil Extracellular Trap (NET) Formation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]