Rave-Fränk, Margret

[["dc.bibliographiccitation.firstpage","731"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","FASEB BioAdvances"],["dc.bibliographiccitation.lastpage","746"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Markov, Alex"],["dc.contributor.author","Kraemer, Lena K."],["dc.contributor.author","Christalla, Peter"],["dc.contributor.author","Rave-Fränk, Margret"],["dc.contributor.author","Fischer, Henrike J."],["dc.contributor.author","Reichardt, Holger Michael"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.date.accessioned","2020-01-21T09:29:21Z"],["dc.date.accessioned","2021-10-27T13:22:07Z"],["dc.date.available","2020-01-21T09:29:21Z"],["dc.date.available","2021-10-27T13:22:07Z"],["dc.date.issued","2019"],["dc.description.abstract","Satellite cells reside in defined niches and are activated upon skeletal muscle injury to facilitate regeneration. Mechanistic studies of skeletal muscle regeneration are hampered by the inability to faithfully simulate satellite cell biology in vitro. We sought to overcome this limitation by developing tissue engineered skeletal muscle (ESM) with (1) satellite cell niches and (2) the capacity to regenerate after injury. ESMs contained quiescent Pax7‐positive satellite cells in morphologically defined niches. Satellite cells could be activated to repair (i) cardiotoxin and (ii) mechanical crush injuries. Activation of the Wnt‐pathway was essential for muscle regeneration. Finally, muscle progenitors from the engineered niche developed de novo ESM in vitro and regenerated skeletal muscle after cardiotoxin‐induced injury in vivo. We conclude that ESM with functional progenitor niches reminiscent of the in vivo satellite cell niches can be engineered in vitro. ESM may ultimately be exploited in disease modeling, drug screening, or muscle regeneration."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2019"],["dc.identifier.doi","10.1096/fba.2019-00013"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17135"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/92070"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","2573-9832"],["dc.relation.issn","2573-9832"],["dc.relation.issn","2573-9832"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Regeneration competent satellite cell niches in rat engineered skeletal muscle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3928"],["dc.bibliographiccitation.issue","31"],["dc.bibliographiccitation.journal","World journal of gastroenterology : WJG"],["dc.bibliographiccitation.lastpage","3935"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Krause, Petra"],["dc.contributor.author","Rave-Fränk, Margret"],["dc.contributor.author","Wolff, Hendrik Andreas"],["dc.contributor.author","Becker, Heinz"],["dc.contributor.author","Christiansen, Hans"],["dc.contributor.author","Koenig, Sarah"],["dc.date.accessioned","2019-07-10T08:13:36Z"],["dc.date.available","2019-07-10T08:13:36Z"],["dc.date.issued","2010"],["dc.description.abstract","AIM: To investigate whether irradiation (IR) and partial hepatectomy (PH) may prepare the host liver for non-parenchymal cell (NPC) transplantation. METHODS: Livers of dipeptidyl peptidase IV (DPPIV)-deficient rats were pre-conditioned with external beam IR (25 Gy) delivered to two-thirds of the right liver lobules followed by a one-third PH of the untreated lobule. DPPIV-positive liver cells (NPC preparations enriched for liver sinusoidal endothelial cells (LSECs) and hepatocytes) were transplanted via the spleen into the recipient livers. The extent and quality of donor cell engraftment and growth was studied over a long-term interval of 16 wk after transplantation. RESULTS: Host liver staining demonstrated 3 different repopulation types. Well defined clusters of donor-derived hepatocytes with canalicular expression of DPPIV were detectable either adjacent to or in between large areas of donor cells (covering up to 90% of the section plane) co-expressing the endothelial marker platelet endothelial cell adhesion molecule. The third type consisted of formations of DPPIV-positive duct-like structures which co-localized with biliary epithelial CD49f. CONCLUSION: Liver IR and PH as a preconditioning stimulus enables multiple cell liver repopulation by donor hepatocytes, LSECs, and bile duct cells."],["dc.identifier.fs","574471"],["dc.identifier.pmid","20712054"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6866"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61286"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1007-9327"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Bile Ducts"],["dc.subject.mesh","Cell Proliferation"],["dc.subject.mesh","Cell Survival"],["dc.subject.mesh","Dipeptidyl Peptidase 4"],["dc.subject.mesh","Endothelial Cells"],["dc.subject.mesh","Hepatectomy"],["dc.subject.mesh","Hepatocytes"],["dc.subject.mesh","Liver"],["dc.subject.mesh","Liver Regeneration"],["dc.subject.mesh","Rats"],["dc.subject.mesh","Rats, Inbred F344"],["dc.subject.mesh","Rats, Transgenic"],["dc.subject.mesh","Time Factors"],["dc.subject.mesh","Transplantation Conditioning"],["dc.title","Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","BioMed Research International"],["dc.bibliographiccitation.lastpage","12"],["dc.bibliographiccitation.volume","2018"],["dc.contributor.author","Ihler, Friedrich"],["dc.contributor.author","Gratz, Ronja"],["dc.contributor.author","Wolff, Hendrik A."],["dc.contributor.author","Weiss, Bernhard G."],["dc.contributor.author","Bertlich, Mattis"],["dc.contributor.author","Kitz, Julia"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Rave-Fränk, Margret"],["dc.contributor.author","Canis, Martin"],["dc.date.accessioned","2019-07-09T11:45:12Z"],["dc.date.available","2019-07-09T11:45:12Z"],["dc.date.issued","2018"],["dc.description.abstract","In epithelial tumors, a shift towards a mesenchymal phenotype has been associated with increased invasiveness andmetastasis. It is assumed that this phenomenon plays amajor role in disease progression and ultimately prognosis.This study investigated epithelialmesenchymal transition (EMT) in human papillomavirus- (HPV-) negative pharyngeal squamous cell carcinoma. Tissue was obtained from one hypopharyngeal primary tumor and a regional lymph nodemetastasis during surgery with curative intention. A cell culture was established fromthe primary tumor andmesenchymal growth conditions were emulated.Gene expression profiling was performed (Human 8 × 60K design array, Agilent Technologies) and EMT was assessed by a gene set (MSigDB: M5930, Hallmark epithelial mesenchymal transition), applying gene set expression analysis (GSEA). Immunohistochemical staining and flow cytometry of CD44 and E-cadherin were compared in primary tumor, metastasis, and cell cultures. Primary tumor and metastasis were highly positive for CD44.Aloss of E-cadherin occurred in the metastasis. Flowcytometry showed the appearance of a population without E-cadherin in spheroid colonies. In GSEA, the EMT phenotype was enriched in the primary tumor compared to metastasis and cell cultures (FDR < 25%, 𝑝 < 5%). EMT showed variable expression during metastasis. It may thereby be a dynamic state in HPV-negative pharyngeal squamous cell carcinoma that is active only during the process of metastasis itself. Thereby, the primary tumor as well as the metastasis may exhibit fewer EMT properties."],["dc.identifier.doi","10.1155/2018/7929104"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15055"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59179"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2314-6141"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Epithelial-Mesenchymal Transition during Metastasis of HPV-Negative Pharyngeal Squamous Cell Carcinoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]