Mißbach-Güntner, Jeannine

[["dc.bibliographiccitation.firstpage","663"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Neoplasia"],["dc.bibliographiccitation.lastpage","673"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Kimmina, Sarah"],["dc.contributor.author","Zientkowska, Marta"],["dc.contributor.author","Domeyer-Missbach, Melanie"],["dc.contributor.author","Malz, Cordula"],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-10T08:11:50Z"],["dc.date.available","2019-07-10T08:11:50Z"],["dc.date.issued","2008"],["dc.description.abstract","Noninvasive methods are strongly needed to detect and quantify not only tumor growth in murine tumor models but also the development of vascularization and necrosis within tumors. This study investigates the use of a new imaging technique, flat-panel detector volume computed tomography (fpVCT), to monitor in vivo tumor progression and structural changes within tumors of two murine carcinoma models. After tumor cell inoculation, single fpVCT scans of the entire mice were performed at different time points. The acquired isotropic, high-resolution volume data sets enable an accurate real-time assessment and precise measurements of tumor volumes. Spreading of contrast agent-containing blood vessels around and within the tumors was clearly visible over time. Furthermore, fpVCT permits the identification of differences in the uptake of contrast media within tumors, thus delineating necrosis, tumor tissues, and blood vessels. Classification of tumor tissues based on the decomposition of the underlying mixture distribution of tissue-related Hounsfield units allowed the quantitative acquisition of necrotic tissues at each time point. Morphologic alterations of the tumor depicted by fpVCT were confirmed by histopathologic examination. Concluding, our data show that fpVCT may be highly suitable for the noninvasive evaluation of tumor responses to anticancer therapies during the course of the disease."],["dc.identifier.doi","10.1593/neo.08270"],["dc.identifier.pii","S1476558608800048"],["dc.identifier.pmid","18592006"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11245"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60806"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/102991"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1476-5586"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.title","Morphologic changes of mammary carcinomas in mice over time as monitored by flat-panel detector volume computed tomography."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e21168"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Sausbier, Ulrike"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Kabagema, Clement"],["dc.contributor.author","Flockerzie, Katarina"],["dc.contributor.author","Kuscher, Gerd Marten"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Neuhuber, Winfried"],["dc.contributor.author","Ruth, Peter"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Sausbier, Matthias"],["dc.date.accessioned","2018-11-07T08:55:07Z"],["dc.date.available","2018-11-07T08:55:07Z"],["dc.date.issued","2011"],["dc.description.abstract","Background: The process of bone resorption by osteoclasts is regulated by Cathepsin K, the lysosomal collagenase responsible for the degradation of the organic bone matrix during bone remodeling. Recently, Cathepsin K was regarded as a potential target for therapeutic intervention of osteoporosis. However, mechanisms leading to osteopenia, which is much more common in young female population and often appears to be the clinical pre-stage of idiopathic osteoporosis, still remain to be elucidated, and molecular targets need to be identified. Methodology/Principal Findings: We found, that in juvenile bone the large conductance, voltage and Ca2+-activated (BK) K+ channel, which links membrane depolarization and local increases in cytosolic calcium to hyperpolarizing K+ outward currents, is exclusively expressed in osteoclasts. In juvenile BK-deficient (BK-/-) female mice, plasma Cathepsin K levels were elevated two-fold when compared to wild-type littermates. This increase was linked to an osteopenic phenotype with reduced bone mineral density in long bones and enhanced porosity of trabecular meshwork in BK-/- vertebrae as demonstrated by high-resolution flat-panel volume computed tomography and micro-CT. However, plasma levels of sRANKL, osteoprotegerin, estrogene, Ca2+ and triiodthyronine as well as osteoclastogenesis were not altered in BK-/- females. Conclusion/Significance: Our findings suggest that the BK channel controls resorptive osteoclast activity by regulating Cathepsin K release. Targeted deletion of BK channel in mice resulted in an osteoclast-autonomous osteopenia, becoming apparent in juvenile females. Thus, the BK-/- mouse-line represents a new model for juvenile osteopenia, and revealed the BK channel as putative new target for therapeutic controlling of osteoclast activity."],["dc.identifier.doi","10.1371/journal.pone.0021168"],["dc.identifier.isi","000291682300031"],["dc.identifier.pmid","21695131"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7820"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22829"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Osteopenia Due to Enhanced Cathepsin K Release by BK Channel Ablation in Osteoclasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","755"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Neoplasia (New York, N.Y.)"],["dc.bibliographiccitation.lastpage","765"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Zientkowska, Marta"],["dc.contributor.author","Domeyer-Missbach, Melanie"],["dc.contributor.author","Kimmina, Sarah"],["dc.contributor.author","Obenauer, Silvia"],["dc.contributor.author","Kauer, Fritz"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Vogel, Wolfgang F."],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-10T08:11:50Z"],["dc.date.available","2019-07-10T08:11:50Z"],["dc.date.issued","2007-09-01"],["dc.description.abstract","Skeletal metastasis is an important cause of mortality in patients with breast cancer. Hence, animal models, in combination with various imaging techniques, are in high demand for preclinical assessment of novel therapies. We evaluated the applicability of flat-panel volume computed tomography (fpVCT) to noninvasive detection of osteolytic bone metastases that develop in severe immunodeficient mice after intracardial injection of MDA-MB-231 breast cancer cells. A single fpVCT scan at 200-microm isotropic resolution was employed to detect osteolysis within the entire skeleton. Osteolytic lesions identified by fpVCT correlated with Faxitron X-ray analysis and were subsequently confirmed by histopathological examination. Isotropic three-dimensional image data sets obtained by fpVCT were the basis for the precise visualization of the extent of the lesion within the cortical bone and for the measurement of bone loss. Furthermore, fpVCT imaging allows continuous monitoring of growth kinetics for each metastatic site and visualization of lesions in more complex regions of the skeleton, such as the skull. Our findings suggest that fpVCT is a powerful tool that can be used to monitor the occurrence and progression of osteolytic lesions in vivo and can be further developed to monitor responses to antimetastatic therapies over the course of the disease."],["dc.identifier.pmid","17898871"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11246"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60807"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1476-5586"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.subject.mesh","Adenocarcinoma"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Bone Neoplasms"],["dc.subject.mesh","Breast Neoplasms"],["dc.subject.mesh","Disease Progression"],["dc.subject.mesh","Female"],["dc.subject.mesh","Femoral Neoplasms"],["dc.subject.mesh","Humerus"],["dc.subject.mesh","Imaging, Three-Dimensional"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Mice, SCID"],["dc.subject.mesh","Models, Animal"],["dc.subject.mesh","Osteolysis"],["dc.subject.mesh","Skull Neoplasms"],["dc.subject.mesh","Specific Pathogen-Free Organisms"],["dc.subject.mesh","Tibia"],["dc.subject.mesh","Tomography, X-Ray Computed"],["dc.subject.mesh","Tumor Burden"],["dc.title","Flat-panel detector-based volume computed tomography: a novel 3D imaging technique to monitor osteolytic bone lesions in a mouse tumor metastasis model."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","286"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","European Journal of Radiology"],["dc.bibliographiccitation.lastpage","293"],["dc.bibliographiccitation.volume","70"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Jannasch, Katharina"],["dc.contributor.author","Mathejczyk, Julia"],["dc.contributor.author","Pardo, Luis A."],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Tietze, Lutz Friedjan"],["dc.date.accessioned","2018-11-07T08:30:16Z"],["dc.date.available","2018-11-07T08:30:16Z"],["dc.date.issued","2009"],["dc.description.abstract","Conventional chemotherapy of cancer has its limitations, especially in advanced and disseminated disease and suffers from lack of specificity. This results in a poor therapeutic index and considerable toxicity to normal organs. Therefore, many efforts are made to develop novel therapeutic tools against cancer with the aim of selectively targeting the drug to the turnout site. Drug delivery strategies fundamentally rely on the identification of good-quality biomarkers, allowing unequivocal discrimination between cancer and healthy tissue. At present, antibodies or antibody fragments have clearly proven their value as carrier molecules specific for a tumour-associated molecular marker. This present review draws attention to the use of near-infrared fluorescence (NIRF) imaging to investigate binding specificity and kinetics of carrier molecules such as monoclonal antibodies. In addition, flat-panel volume computed tomography (fpVCT) will be presented to monitor anatomical structures in turnout mouse models over time in a non-invasive manner. Each imaging device sheds light on a different aspect; functional imaging is applied to optimise the dose schedule and the concept of selective tumour therapies, whereas anatomical imaging assesses preclinically the efficacy of novel turnout therapies. Both imaging techniques in combination allow the visualisation of functional information obtained by NIRF imaging within an adequate anatomic framework. (C) 2009 Elsevier Ireland Ltd. All rights reserved."],["dc.description.sponsorship","DFG [SFB 416, AL336/5-1]"],["dc.identifier.doi","10.1016/j.ejrad.2009.01.048"],["dc.identifier.isi","000266868900012"],["dc.identifier.pmid","19285818"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16849"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","0720-048X"],["dc.title","Concept of a selective tumour therapy and its evaluation by near-infrared fluorescence imaging and flat-panel volume computed tomography in mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]