Mißbach-Güntner, Jeannine

[["dc.bibliographiccitation.artnumber","andr.13292"],["dc.bibliographiccitation.firstpage","1660"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Andrology"],["dc.bibliographiccitation.lastpage","1672"],["dc.bibliographiccitation.volume","10"],["dc.contributor.affiliation","Pinkert‐Leetsch, Diana; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Rost, John Uwe; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Schmiedeknecht, Max Ulrich Heiner; 3\r\nDepartment of Neuropathology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Stadelmann, Christine; 3\r\nDepartment of Neuropathology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Alves, Frauke; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.author","Pinkert‐Leetsch, Diana"],["dc.contributor.author","Rost, John Uwe"],["dc.contributor.author","Schmiedeknecht, Max Ulrich Heiner"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Missbach‐Guentner, Jeannine"],["dc.date.accessioned","2022-11-28T08:48:04Z"],["dc.date.available","2022-11-28T08:48:04Z"],["dc.date.issued","2022-09-15"],["dc.date.updated","2022-11-27T10:10:46Z"],["dc.description.abstract","Abstract\r\n\r\nBackground\r\nThe unique anatomy of the male reproductive organ reflects its complex function from sperm maturation to their storage for months until emission. Since light microscopy in two dimensions (2d) cannot sufficiently demonstrate its complex morphology, a comprehensive visualization is required to identify pathologic alterations in its entire anatomical context.\r\n\r\n\r\nObjectives\r\nAim of this study was to use three‐dimensional (3d) light sheet fluorescence microscopy (LSFM) to visualize entire murine testes in 3d, label‐free and at subcellular resolution, and to assign local autofluorescence to testicular and deferent structures.\r\n\r\n\r\nMaterials and methods\r\nMurine testes were fixed with four different fixatives and subsequently cleared with benzoic acid/benzyl benzoate. Hereafter, complete murine testes were scanned with LSFM with different fluorescence filter sets and subsequently embedded in paraffin for further conventional planar histology.\r\n\r\n\r\nResults\r\nAutofluorescence signals of the murine reproductive organ allowed the unambiguous identification of the testicular anatomy from the seminiferous tubules to the vas deferens with their specific stratification independent of the used fixative. Blood vessels were visualized from the pampiniform plexus to the small capillaries of single tubules. Moreover, due to the specific intrinsic fluorescence properties of the efferent ducts and the epididymis, luminal caliber, the epithelial stratification and retronuclear cytoplasmic inclusions gave a unique insight into the interface of both morphological structures. Subsequent 2d histology confirmed the identified morphological structures.\r\n\r\n\r\nDiscussion\r\nLSFM analysis of the murine reproductive organ allows due to its intrinsic fluorescence a simple, label‐free 3d assessment of its entire duct morphology, the epithelial composition, and the associated blood supply in its anatomical relation.\r\n\r\n\r\nConclusion\r\nLSFM provides the technical basis for comprehensive analyses of pathologically altered murine testes in its entirety by depicting specific autofluorescence. Thereby it facilitates mouse studies of testicular disease or their drug‐related alterations in more detail potentially for clinical translation assessing human testicular biopsies."],["dc.description.sponsorship","Bundesministerium fuer Bildung und Forschung, Deutschland"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1111/andr.13292"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/117280"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","2047-2927"],["dc.relation.issn","2047-2919"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made."],["dc.title","The murine male reproductive organ at a glance: Three‐dimensional insights and virtual histology using label‐free light sheet microcopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e118"],["dc.bibliographiccitation.firstpage","1232"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PLoS Genetics"],["dc.bibliographiccitation.lastpage","1243"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Vogel, Wolfgang F."],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2018-11-07T11:01:18Z"],["dc.date.available","2018-11-07T11:01:18Z"],["dc.date.issued","2007"],["dc.description.abstract","Rapid progress in exploring the human and mouse genome has resulted in the generation of a multitude of mouse models to study gene functions in their biological context. However, effective screening methods that allow rapid noninvasive phenotyping of transgenic and knockout mice are still lacking. To identify murine models with bone alterations in vivo, we used flat-panel volume computed tomography (fpVCT) for high-resolution 3-D imaging and developed an algorithm with a computational intelligence system. First, we tested the accuracy and reliability of this approach by imaging discoidin domain receptor 2-(DDR2-) deficient mice, which display distinct skull abnormalities as shown by comparative landmark-based analysis. High-contrast fpVCT data of the skull with 200 mu m isotropic resolution and 8-s scan time allowed segmentation and computation of significant shape features as well as visualization of morphological differences. The application of a trained artificial neuronal network to these datasets permitted a semiautomatic and highly accurate phenotype classification of DDR2-deficient compared to C57BL/6 wild-type mice. Even heterozygous DDR2 mice with only subtle phenotypic alterations were correctly determined by fpVCT imaging and identified as a new class. In addition, we successfully applied the algorithm to classify knockout mice lacking the DDR1 gene with no apparent skull deformities. Thus, this new method seems to be a potential tool to identify novel mouse phenotypes with skull changes from transgenic and knockout mice on the basis of random mutagenesis as well as from genetic models. However for this purpose, new neuronal networks have to be created and trained. In summary, the combination of fpVCT images with artificial neuronal networks provides a reliable, novel method for rapid, cost-effective, and noninvasive primary screening tool to detect skeletal phenotypes in mice."],["dc.identifier.doi","10.1371/journal.pgen.0030118"],["dc.identifier.isi","000248350000010"],["dc.identifier.pmid","17658952"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8443"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51120"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1553-7390"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Semi-automatic classification of skeletal morphology in genetically altered mice using flat-panel volume computed tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1407"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Pinkert-Leetsch, Diana"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Ufartes, Roser"],["dc.contributor.author","Hornung, Daniel"],["dc.contributor.author","Tampe, Bjoern"],["dc.contributor.author","Zeisberg, Michael"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-09T11:45:05Z"],["dc.date.available","2019-07-09T11:45:05Z"],["dc.date.issued","2018"],["dc.description.abstract","The increasing number of patients with end stage chronic kidney disease not only calls for novel therapeutics but also for pioneering research using convincing preclinical disease models and innovative analytical techniques. The aim of this study was to introduce a virtual histology approach using micro computed tomography (µCT) for the entire murine kidney in order to close the gap between single slice planar histology and a 3D high resolution dataset. An ex vivo staining protocol based on phosphotungstic acid diffusion was adapted to enhance renal soft tissue x-ray attenuation. Subsequent CT scans allowed (i) the detection of the renal cortex, medulla and pelvis in greater detail, (ii) the analysis of morphological alterations, (iii) the quantification of the volume as well as the radio-opacity of these portions and (iv) the quantification of renal fibrotic remodeling based on altered radio-opacity using the unilateral ureteral obstruction model. Thus, virtual histology based on PTA contrast enhanced CT will in future help to refine the outcome of preclinical research on kidney associated murine disease models."],["dc.identifier.doi","10.1038/s41598-018-19773-5"],["dc.identifier.pmid","29362427"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15031"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59157"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","3D virtual histology of murine kidneys -high resolution visualization of pathological alterations by micro computed tomography."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0170597"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Ufartes, Roser"],["dc.contributor.author","Larsson, Emanuel"],["dc.contributor.author","Martin, Sabine"],["dc.contributor.author","Lazzarini, Marcio"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Pinkert-Leetsch, Diana"],["dc.contributor.author","Katschinski, Doerthe Magdalena"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2018-11-07T10:27:31Z"],["dc.date.available","2018-11-07T10:27:31Z"],["dc.date.issued","2017"],["dc.description.abstract","The small size of the adult and developing mouse heart poses a great challenge for imaging in preclinical research. The aim of the study was to establish a phosphotungstic acid (PTA) ex-vivo staining approach that efficiently enhances the x-ray attenuation of soft-tissue to allow high resolution 3D visualization of mouse hearts by synchrotron radiation based pCT (SRpCT) and classical pCT. We demonstrate that SRpCT of PTA stained mouse hearts ex vivo allows imaging of the cardiac atrium, ventricles, myocardium especially its fibre structure and vessel walls in great detail and furthermore enables the depiction of growth and anatomical changes during distinct developmental stages of hearts in mouse embryos. Our x-ray based virtual histology approach is not limited to SRpCT as it does not require monochromatic and/or coherent x-ray sources and even more importantly can be combined with conventional histological procedures. Furthermore, it permits volumetric measurements as we show for the assessment of the plaque volumes in the aortic valve region of mice from an ApoE-/- mouse model. Subsequent, Masson-Goldner trichrome staining of paraffin sections of PTA stained samples revealed intact collagen and muscle fibres and positive staining of CD31 on endothelial cells by immunohistochemistry illustrates that our approach does not prevent immunochemistry analysis. The feasibility to scan hearts already embedded in paraffin ensured a 100% correlation between virtual cut sections of the CT data sets and histological heart sections of the same sample and may allow in future guiding the cutting process to specific regions of interest. In summary, since our CT based virtual histology approach is a powerful tool for the 3D depiction of morphological alterations in hearts and embryos in high resolution and can be combined with classical histological analysis it may be used in preclinical research to unravel structural alterations of various heart diseases."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1371/journal.pone.0170597"],["dc.identifier.isi","000393712500011"],["dc.identifier.pmid","28178293"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14241"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43247"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","mu CT of ex-vivo stained mouse hearts and embryos enables a precise match between 3D virtual histology, classical histology and immunochemistry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","663"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Neoplasia"],["dc.bibliographiccitation.lastpage","673"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Kimmina, Sarah"],["dc.contributor.author","Zientkowska, Marta"],["dc.contributor.author","Domeyer-Missbach, Melanie"],["dc.contributor.author","Malz, Cordula"],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-10T08:11:50Z"],["dc.date.available","2019-07-10T08:11:50Z"],["dc.date.issued","2008"],["dc.description.abstract","Noninvasive methods are strongly needed to detect and quantify not only tumor growth in murine tumor models but also the development of vascularization and necrosis within tumors. This study investigates the use of a new imaging technique, flat-panel detector volume computed tomography (fpVCT), to monitor in vivo tumor progression and structural changes within tumors of two murine carcinoma models. After tumor cell inoculation, single fpVCT scans of the entire mice were performed at different time points. The acquired isotropic, high-resolution volume data sets enable an accurate real-time assessment and precise measurements of tumor volumes. Spreading of contrast agent-containing blood vessels around and within the tumors was clearly visible over time. Furthermore, fpVCT permits the identification of differences in the uptake of contrast media within tumors, thus delineating necrosis, tumor tissues, and blood vessels. Classification of tumor tissues based on the decomposition of the underlying mixture distribution of tissue-related Hounsfield units allowed the quantitative acquisition of necrotic tissues at each time point. Morphologic alterations of the tumor depicted by fpVCT were confirmed by histopathologic examination. Concluding, our data show that fpVCT may be highly suitable for the noninvasive evaluation of tumor responses to anticancer therapies during the course of the disease."],["dc.identifier.doi","10.1593/neo.08270"],["dc.identifier.pii","S1476558608800048"],["dc.identifier.pmid","18592006"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11245"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60806"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/102991"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1476-5586"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.title","Morphologic changes of mammary carcinomas in mice over time as monitored by flat-panel detector volume computed tomography."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e21168"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Sausbier, Ulrike"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Kabagema, Clement"],["dc.contributor.author","Flockerzie, Katarina"],["dc.contributor.author","Kuscher, Gerd Marten"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Neuhuber, Winfried"],["dc.contributor.author","Ruth, Peter"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Sausbier, Matthias"],["dc.date.accessioned","2018-11-07T08:55:07Z"],["dc.date.available","2018-11-07T08:55:07Z"],["dc.date.issued","2011"],["dc.description.abstract","Background: The process of bone resorption by osteoclasts is regulated by Cathepsin K, the lysosomal collagenase responsible for the degradation of the organic bone matrix during bone remodeling. Recently, Cathepsin K was regarded as a potential target for therapeutic intervention of osteoporosis. However, mechanisms leading to osteopenia, which is much more common in young female population and often appears to be the clinical pre-stage of idiopathic osteoporosis, still remain to be elucidated, and molecular targets need to be identified. Methodology/Principal Findings: We found, that in juvenile bone the large conductance, voltage and Ca2+-activated (BK) K+ channel, which links membrane depolarization and local increases in cytosolic calcium to hyperpolarizing K+ outward currents, is exclusively expressed in osteoclasts. In juvenile BK-deficient (BK-/-) female mice, plasma Cathepsin K levels were elevated two-fold when compared to wild-type littermates. This increase was linked to an osteopenic phenotype with reduced bone mineral density in long bones and enhanced porosity of trabecular meshwork in BK-/- vertebrae as demonstrated by high-resolution flat-panel volume computed tomography and micro-CT. However, plasma levels of sRANKL, osteoprotegerin, estrogene, Ca2+ and triiodthyronine as well as osteoclastogenesis were not altered in BK-/- females. Conclusion/Significance: Our findings suggest that the BK channel controls resorptive osteoclast activity by regulating Cathepsin K release. Targeted deletion of BK channel in mice resulted in an osteoclast-autonomous osteopenia, becoming apparent in juvenile females. Thus, the BK-/- mouse-line represents a new model for juvenile osteopenia, and revealed the BK channel as putative new target for therapeutic controlling of osteoclast activity."],["dc.identifier.doi","10.1371/journal.pone.0021168"],["dc.identifier.isi","000291682300031"],["dc.identifier.pmid","21695131"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7820"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22829"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Osteopenia Due to Enhanced Cathepsin K Release by BK Channel Ablation in Osteoclasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","755"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Neoplasia (New York, N.Y.)"],["dc.bibliographiccitation.lastpage","765"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Zientkowska, Marta"],["dc.contributor.author","Domeyer-Missbach, Melanie"],["dc.contributor.author","Kimmina, Sarah"],["dc.contributor.author","Obenauer, Silvia"],["dc.contributor.author","Kauer, Fritz"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Vogel, Wolfgang F."],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-10T08:11:50Z"],["dc.date.available","2019-07-10T08:11:50Z"],["dc.date.issued","2007-09-01"],["dc.description.abstract","Skeletal metastasis is an important cause of mortality in patients with breast cancer. Hence, animal models, in combination with various imaging techniques, are in high demand for preclinical assessment of novel therapies. We evaluated the applicability of flat-panel volume computed tomography (fpVCT) to noninvasive detection of osteolytic bone metastases that develop in severe immunodeficient mice after intracardial injection of MDA-MB-231 breast cancer cells. A single fpVCT scan at 200-microm isotropic resolution was employed to detect osteolysis within the entire skeleton. Osteolytic lesions identified by fpVCT correlated with Faxitron X-ray analysis and were subsequently confirmed by histopathological examination. Isotropic three-dimensional image data sets obtained by fpVCT were the basis for the precise visualization of the extent of the lesion within the cortical bone and for the measurement of bone loss. Furthermore, fpVCT imaging allows continuous monitoring of growth kinetics for each metastatic site and visualization of lesions in more complex regions of the skeleton, such as the skull. Our findings suggest that fpVCT is a powerful tool that can be used to monitor the occurrence and progression of osteolytic lesions in vivo and can be further developed to monitor responses to antimetastatic therapies over the course of the disease."],["dc.identifier.pmid","17898871"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11246"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60807"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1476-5586"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.subject.mesh","Adenocarcinoma"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Bone Neoplasms"],["dc.subject.mesh","Breast Neoplasms"],["dc.subject.mesh","Disease Progression"],["dc.subject.mesh","Female"],["dc.subject.mesh","Femoral Neoplasms"],["dc.subject.mesh","Humerus"],["dc.subject.mesh","Imaging, Three-Dimensional"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Mice, SCID"],["dc.subject.mesh","Models, Animal"],["dc.subject.mesh","Osteolysis"],["dc.subject.mesh","Skull Neoplasms"],["dc.subject.mesh","Specific Pathogen-Free Organisms"],["dc.subject.mesh","Tibia"],["dc.subject.mesh","Tomography, X-Ray Computed"],["dc.subject.mesh","Tumor Burden"],["dc.title","Flat-panel detector-based volume computed tomography: a novel 3D imaging technique to monitor osteolytic bone lesions in a mouse tumor metastasis model."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1460"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","1466"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Pacilè, Serena"],["dc.contributor.author","Baran, Patrycja"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Dimmock, Matthew"],["dc.contributor.author","Lockie, Darren"],["dc.contributor.author","Missbach-Guntner, Jeannine"],["dc.contributor.author","Quiney, Harry"],["dc.contributor.author","McCormack, Mikkaela"],["dc.contributor.author","Mayo, Sheridan"],["dc.contributor.author","Thompson, Darren"],["dc.contributor.author","Nesterets, Yakov"],["dc.contributor.author","Hall, Chris"],["dc.contributor.author","Pavlov, Konstantin"],["dc.contributor.author","Prodanovic, Zdenka"],["dc.contributor.author","Tonutti, Maura"],["dc.contributor.author","Accardo, Agostino"],["dc.contributor.author","Fox, Jane"],["dc.contributor.author","Tavakoli Taba, Seyedamir"],["dc.contributor.author","Lewis, Sarah"],["dc.contributor.author","Brennan, Patrick"],["dc.contributor.author","Hausermann, Daniel"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Gureyev, Tim"],["dc.date.accessioned","2020-12-10T18:25:58Z"],["dc.date.available","2020-12-10T18:25:58Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1107/S1600577518010172"],["dc.identifier.eissn","1600-5775"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75898"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Advantages of breast cancer visualization and characterization using synchrotron radiation phase-contrast tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","286"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","European Journal of Radiology"],["dc.bibliographiccitation.lastpage","293"],["dc.bibliographiccitation.volume","70"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Jannasch, Katharina"],["dc.contributor.author","Mathejczyk, Julia"],["dc.contributor.author","Pardo, Luis A."],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Tietze, Lutz Friedjan"],["dc.date.accessioned","2018-11-07T08:30:16Z"],["dc.date.available","2018-11-07T08:30:16Z"],["dc.date.issued","2009"],["dc.description.abstract","Conventional chemotherapy of cancer has its limitations, especially in advanced and disseminated disease and suffers from lack of specificity. This results in a poor therapeutic index and considerable toxicity to normal organs. Therefore, many efforts are made to develop novel therapeutic tools against cancer with the aim of selectively targeting the drug to the turnout site. Drug delivery strategies fundamentally rely on the identification of good-quality biomarkers, allowing unequivocal discrimination between cancer and healthy tissue. At present, antibodies or antibody fragments have clearly proven their value as carrier molecules specific for a tumour-associated molecular marker. This present review draws attention to the use of near-infrared fluorescence (NIRF) imaging to investigate binding specificity and kinetics of carrier molecules such as monoclonal antibodies. In addition, flat-panel volume computed tomography (fpVCT) will be presented to monitor anatomical structures in turnout mouse models over time in a non-invasive manner. Each imaging device sheds light on a different aspect; functional imaging is applied to optimise the dose schedule and the concept of selective tumour therapies, whereas anatomical imaging assesses preclinically the efficacy of novel turnout therapies. Both imaging techniques in combination allow the visualisation of functional information obtained by NIRF imaging within an adequate anatomic framework. (C) 2009 Elsevier Ireland Ltd. All rights reserved."],["dc.description.sponsorship","DFG [SFB 416, AL336/5-1]"],["dc.identifier.doi","10.1016/j.ejrad.2009.01.048"],["dc.identifier.isi","000266868900012"],["dc.identifier.pmid","19285818"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16849"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","0720-048X"],["dc.title","Concept of a selective tumour therapy and its evaluation by near-infrared fluorescence imaging and flat-panel volume computed tomography in mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Imaging"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Zientkowska, Marta"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Missbach-Guentner, Jeannine"],["dc.contributor.author","Krell, Hans-Willi"],["dc.contributor.author","Mueller, Friedernann"],["dc.contributor.author","Grabbe, Eckhardt"],["dc.contributor.author","Tietze, Lutz Friedjan"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2018-11-07T08:35:08Z"],["dc.date.available","2018-11-07T08:35:08Z"],["dc.date.issued","2009"],["dc.description.abstract","Connecting fluorescence signals with anatomic structures enhances our ability to monitor biologic processes in mice. Here, we present a semiautomated approach to correlate two-dimensional (2D) noninvasive near-infrared fluorescence (NIRF) imaging with three-dimensional (3D), high-resolution, flat-panel volume computed tomography (fpVCT). We developed an algorithm to colocalize fluorescence signals of NIRF-labeled antibodies directed against matriptase and urokinase plasminogen activator receptor (uPAR) to orthotopic carcinomas in mice visualized by fpVCT. For this purpose, mice were anesthetized and fixed on a multimodality animal bed containing fiducial markers filled with iodine-containing contrast agent and fluorescent dye. After intravenous administration of contrast agent and Cy5.5-labeled antibodies, NIRF and fpVCT images were obtained, without repositioning the mice. Binding of Cy5.5-labeled matriptase-specific antibody to pancreatic tumors and Cy5.5-labeled uPAR-specific antibody to mammary carcinomas was assessed by time-domain NIRF imaging measuring the location of fluorescence intensity and its lifetime. In summary, we developed a novel 2D-3D registration technique for image fusion with NIRF imaging and fpVCT to provide complementary information in tumor models on the in vivo association of functional information with anatomic structures. The combination of fpVCT with NIRF imaging will now allow targeted and effective monitoring of preclinical tumor therapies."],["dc.identifier.doi","10.2310/7290.2009.00001"],["dc.identifier.isi","000263883600001"],["dc.identifier.pmid","19344571"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17988"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","B C Decker Inc"],["dc.relation.issn","1535-3508"],["dc.title","Semiautomatic Landmark-Based Two-Dimensional-Three-Dimensional Image Fusion in Living Mice: Correlation of Near-Infrared Fluorescence Imaging of Cy5.5-Labeled Antibodies with Flat-Panel Volume Computed Tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]