Geil, Burkhard

[["dc.bibliographiccitation.firstpage","2216"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2228"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Verbeek, Sarah"],["dc.contributor.author","Bao, Chunxiao"],["dc.contributor.author","Thiart, Jan"],["dc.contributor.author","Kros, Alexander"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:14:08Z"],["dc.date.available","2018-11-07T10:14:08Z"],["dc.date.issued","2016"],["dc.description.abstract","The fusion of lipid membranes is a key process in biology. It enables cells and organelles to exchange molecules with their surroundings, which otherwise could not cross the membrane barrier. To study such complex processes we use simplified artificial model systems, i.e., an optical fusion assay based on membrane-coated glass spheres. We present a technique to analyze membrane-membrane interactions in a large ensemble of particles. Detailed information on the geometry of the fusion stalk of fully fused membranes is obtained by studying the diffusional lipid dynamics with fluorescence recovery after photo-bleaching experiments. A small contact zone is a strong obstruction for the particle exchange across the fusion spot. With the aid of computer simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-membrane-coated beads allow us to estimate the size of the contact zones between two membrane-coated beads. Minimizing delamination and bending energy leads to minimal angles close to those geometrically allowed."],["dc.description.sponsorship","SFB [803 (B08)]"],["dc.identifier.doi","10.1016/j.bpj.2016.04.026"],["dc.identifier.isi","000376436700012"],["dc.identifier.pmid","27224487"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40569"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12006"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","15"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schwenen, Lando L. G."],["dc.contributor.author","Hubrich, Raphael"],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Yang, Jian"],["dc.contributor.author","Kros, Alexander"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:43:42Z"],["dc.date.available","2017-09-07T11:43:42Z"],["dc.date.issued","2015"],["dc.description.abstract","Even though a number of different in vitro fusion assays have been developed to analyze protein mediated fusion, they still only partially capture the essential features of the in vivo situation. Here we established an in vitro fusion assay that mimics the fluidity and planar geometry of the cellular plasma membrane to be able to monitor fusion of single protein-containing vesicles. As a proof of concept, planar pore-spanning membranes harboring SNARE-proteins were generated on highly ordered functionalized 1.2 mu m-sized pore arrays in Si3N4. Full mobility of the membrane components was demonstrated by fluorescence correlation spectroscopy. Fusion was analyzed by two color confocal laser scanning fluorescence microscopy in a time resolved manner allowing to readily distinguish between vesicle docking, intermediate states such as hemifusion and full fusion. The importance of the membrane geometry on the fusion process was highlighted by comparing SNARE-mediated fusion with that of a minimal SNARE fusion mimetic."],["dc.identifier.doi","10.1038/srep12006"],["dc.identifier.fs","613746"],["dc.identifier.gro","3141864"],["dc.identifier.isi","000357847500001"],["dc.identifier.pmid","26165860"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13641"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1923"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG [SFB 803, B04, B05]; Chinese Scholarship Council (CSC)"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Biomimetic Materials"],["dc.subject.mesh","Fluorescent Dyes"],["dc.subject.mesh","Gold"],["dc.subject.mesh","Kinetics"],["dc.subject.mesh","Membrane Fusion"],["dc.subject.mesh","Microscopy, Confocal"],["dc.subject.mesh","Models, Biological"],["dc.subject.mesh","Porosity"],["dc.subject.mesh","Rats"],["dc.subject.mesh","Recombinant Proteins"],["dc.subject.mesh","SNARE Proteins"],["dc.subject.mesh","Silicon Compounds"],["dc.subject.mesh","Time-Lapse Imaging"],["dc.subject.mesh","Unilamellar Liposomes"],["dc.title","Resolving single membrane fusion events on planar pore-spanning membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]