Paul, Thomas

[["dc.bibliographiccitation.artnumber","10"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular and cellular pediatrics"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Sigler, Matthias"],["dc.contributor.author","Klötzer, Julia"],["dc.contributor.author","Quentin, Thomas"],["dc.contributor.author","Paul, Thomas"],["dc.contributor.author","Möller, Oliver"],["dc.date.accessioned","2019-07-09T11:41:55Z"],["dc.date.available","2019-07-09T11:41:55Z"],["dc.date.issued","2015-12-01"],["dc.description.abstract","BACKGROUND: Stent implantation into the tracheo-bronchial system may be life-saving in selected pediatric patients with otherwise intractable stenosis of the upper airways. Following implantation, significant tissue proliferation may occur, requiring re-interventions. We sought to evaluate the effect of immunosuppressive coating of the stents on the extent of tissue proliferation in an animal model. METHODS: Bare metal and sirolimus-coated stents (Bx Sonic and Cypher Select, Johnson & Johnson, Cordis) were implanted into non-stenotic lower airways of New Zealand white rabbits (weight 3.1 to 4.8 kg). Three stents with sirolimus coating and six bare metal stents could be analyzed by means of histology and immunohistochemistry 12 months after implantation. RESULTS: On a macroscopic evaluation, all stents were partially covered with a considerable amount of whitish tissue. Histologically, these proliferations contained fiber-rich connective tissue and some fibromuscular cells without significant differences between both stent types. The superficial tissue layer was formed by typical respiratory epithelium and polygonal cells. Abundant lymphocyte infiltrations and moderate granulocyte infiltrations were found in both groups correspondingly, whereas foreign-body reaction was more pronounced around sirolimus-eluting stents. CONCLUSIONS: After stent implantation in the tracheo-bronchial system of rabbits, we found tissue reactions comparable to those seen after stent implantation into the vascular system. There was no difference between coated and uncoated stents with regard to quality and quantity of tissue proliferation. We found, however, a significantly different inflammatory reaction with a more pronounced foreign-body reaction in sirolimus-coated stents. In our small series, drug-eluting stents did not exhibit any benefit over bare metal stents in an experimental setting."],["dc.identifier.doi","10.1186/s40348-015-0021-7"],["dc.identifier.pmid","26542300"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12586"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58548"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2194-7791"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Stent implantation into the tracheo-bronchial system in rabbits: histopathologic sequelae in bare metal vs. drug-eluting stents."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular and Cellular Pediatrics"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Krause, Ulrich"],["dc.contributor.author","Alflen, Christian"],["dc.contributor.author","Steinmetz, Michael"],["dc.contributor.author","Müller, Matthias J."],["dc.contributor.author","Quentin, Thomas"],["dc.contributor.author","Paul, Thomas"],["dc.date.accessioned","2019-07-09T11:41:09Z"],["dc.date.available","2019-07-09T11:41:09Z"],["dc.date.issued","2015"],["dc.description.abstract","Background Sodium channels predominantly expressed in brain are expressed in myocardial tissue and play an important role in cardiac physiology. Alterations of sodium channels are known to result in neurological disease in infancy and childhood. It will be of interest to study the expression of brain-type sodium channels in the developing myocardium. Methods The expression of neuronal sodium channels (SCN1A, SCN8A) and the cardiac isoform SCN5A in the developing rat myocardium was studied by rtPCR, Western blot, and immunohistochemistry at different stages of antenatal and postnatal development. Results Significant changes of sodium channel expression during development were detected. Whereas SCN5A RNA increased to maximum levels on day 21 after birth, the highest SCN1A RNA levels were detected on day 1 to 7 after birth. SCN8A RNA was maximally expressed during embryonic development. At the protein level, the amount of SCN5A protein increased along with the RNA level. SCN1A protein level decreased after birth in contrast to RNA expression. Western blot could not detect SCN8A protein in the myocardium at any stage of development. Immunohistochemistry however proved the presence of SCN8A protein in the developing rat myocardium. Conclusions Heart- and brain-type sodium channels are differentially expressed during ontogenesis. The high expression level of SCN1A in the perinatal period and early infancy indicates its importance in preserving a regular cardiac rhythm in this early phase of life. Altered regulation of sodium channels might result in severe cardiac rhythm disturbances."],["dc.identifier.doi","10.1186/s40348-015-0015-5"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11734"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58360"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2194-7791"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Characterization of maturation of neuronal voltage-gated sodium channels SCN1A and SCN8A in rat myocardium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]