Hell, Stefan W.

[["dc.bibliographiccitation.artnumber","042004"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Methods and Applications in Fluorescence"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Sednev, Maksim V."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:49Z"],["dc.date.available","2017-09-07T11:54:49Z"],["dc.date.issued","2015"],["dc.description.abstract","The review deals with commercially available organic dyes possessing large Stokes shifts and their applications as fluorescent labels in optical microscopy based on stimulated emission depletion (STED). STED microscopy breaks Abbe's diffraction barrier and provides optical resolution beyond the diffraction limit. STED microscopy is non-invasive and requires photostable fluorescent markers attached to biomolecules or other objects of interest. Up to now, in most biology-related STED experiments, bright and photoresistant dyes with small Stokes shifts of 20-40 nm were used. The rapid progress in STED microscopy showed that organic fluorophores possessing large Stokes shifts are indispensable in multi-color super-resolution techniques. The ultimate result of the imaging relies on the optimal combination of a dye, the bio-conjugation procedure and the performance of the optical microscope. Modern bioconjugation methods, basics of STED microscopy, as well as structures and spectral properties of the presently available fluorescent markers are reviewed and discussed. In particular, the spectral properties of the commercial dyes are tabulated and correlated with the available depletion wavelengths found in STED microscopes produced by LEICA Microsytems, Abberior Instruments and Picoquant GmbH."],["dc.identifier.doi","10.1088/2050-6120/3/4/042004"],["dc.identifier.gro","3141768"],["dc.identifier.isi","000380688100007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/857"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2050-6120"],["dc.title","Fluorescent dyes with large Stokes shifts for super-resolution optical microscopy of biological objects: a review"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","337"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","European Journal of Organic Chemistry"],["dc.bibliographiccitation.lastpage","349"],["dc.bibliographiccitation.volume","2015"],["dc.contributor.author","Mitronova, Gyuzel Yu"],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Wolfram, Thomas"],["dc.contributor.author","Meineke, Dirk N. H."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","John, Michael"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:46Z"],["dc.date.available","2017-09-07T11:44:46Z"],["dc.date.issued","2015"],["dc.description.abstract","Aromatic nucleophilic substitution (SNAr) of fluorine in 9-(3'-carboxy-4',5',6',7'-tetrafluorophenyl) groups of xanthene dyes constitutes a powerful tool in dye design. Thiols and amines regioselectively replace F-6'. This approach enables additional hydrophilic residues or functional groups required for bioconjugation to be introduced. By using this methodology, a \"bright\" and photostable dye for two-color superresolution microscopy was synthesized (with absorption and emission maxima at 604 and 627 nm, respectively). In the case of red-emitting rhodamine dyes with 3'-carboxy-4',5',7'-trifluorophenyl residues, two-dimensional NMR techniques and a chemical transformation were used to prove the precise position of the additional substituent - a carboxylic acid group linked through the S-atom at C-6'. Furthermore, simple H-1 NMR spectra reliably permit the position of the additional carboxy substituent in the 3'-carboxyphenyl ring (at C-5' or C-6') to be established. Information on the exact position of this substituent is significant for the design of molecular probes and for the prediction of the properties of their bioconjugates."],["dc.identifier.doi","10.1002/ejoc.201403269"],["dc.identifier.gro","3141988"],["dc.identifier.isi","000347722900011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3301"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1099-0690"],["dc.relation.issn","1434-193X"],["dc.title","Functionalization of the meso-Phenyl Ring of Rhodamine Dyes Through SNAr with Sulfur Nucleophiles: Synthesis, Biophysical Characterizations, and Comprehensive NMR Analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1651"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1660"],["dc.bibliographiccitation.volume","101"],["dc.contributor.author","Mueller, Veronica"],["dc.contributor.author","Ringemann, Christian"],["dc.contributor.author","Honigmann, Alf"],["dc.contributor.author","Schwarzmann, Guenter"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Leuschner, Ivo"],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:43:23Z"],["dc.date.available","2017-09-07T11:43:23Z"],["dc.date.issued","2011"],["dc.description.abstract","Details about molecular membrane dynamics in living cells, such as lipid-protein interactions, are often hidden from the observer because of the limited spatial resolution of conventional far-field optical microscopy. The superior spatial resolution of stimulated emission depletion (STED) nanoscopy can provide new insights into this process. The application of fluorescence correlation spectroscopy (FCS) in focal spots continuously tuned down to 30 nm in diameter distinguishes between free and anomalous molecular diffusion due to, for example, transient binding of lipids to other membrane constituents, such as lipids and proteins. We compared STED-FCS data recorded on various fluorescent lipid analogs in the plasma membrane of living mammalian cells. Our results demonstrate details about the observed transient formation of molecular complexes. The diffusion characteristics of phosphoglycerolipids without hydroxyl-containing headgroups revealed weak interactions. The strongest interactions were observed with sphingolipid analogs, which showed cholesterol-assisted and cytoskeleton-dependent binding. The hydroxyl-containing headgroup of gangliosides, galactosylceramide, and phosphoinositol assisted binding, but in a much less cholesterol- and cytoskeleton-dependent manner. The observed anomalous diffusion indicates lipid-specific transient hydrogen bonding to other membrane molecules, such as proteins, and points to a distinct connectivity of the various lipids to other membrane constituents. This strong interaction is different from that responsible for forming cholesterol-dependent, liquid-ordered domains in model membranes."],["dc.identifier.doi","10.1016/j.bpj.2011.09.006"],["dc.identifier.gro","3142651"],["dc.identifier.isi","000295661300011"],["dc.identifier.pmid","21961591"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7462"],["dc.bibliographiccitation.issue","44"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","7465"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:53:30Z"],["dc.date.available","2017-09-07T11:53:30Z"],["dc.date.issued","2006"],["dc.description.abstract","I see red, I see red, I see red: Optically switchable compounds were synthesized whose visible fluorescence can be quenched down to 6 % with light at 360–400 nm and then restored with red light from a diode laser. The molecular switch offers potential applications in data storage and far-field microscopy with spatial resolution far below the diffraction limit."],["dc.identifier.doi","10.1002/anie.200602591"],["dc.identifier.gro","3143754"],["dc.identifier.isi","000242285700035"],["dc.identifier.pmid","17042053"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1303"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1433-7851"],["dc.title","Reversible red fluorescent molecular switches"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","522"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Photochemical & Photobiological Sciences"],["dc.bibliographiccitation.lastpage","532"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Sednev, Maksim V."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:43:09Z"],["dc.date.available","2017-09-07T11:43:09Z"],["dc.date.issued","2012"],["dc.description.abstract","Caged near-IR emitting fluorescent dyes are in high demand in optical microscopy but up to now were unavailable. We discovered that the combination of a carbopyronine dye core and a photosensitive 2-diazo-1-indanone residue leads to masked near-IR emitting fluorescent dyes. Illumination of these caged dyes with either UV or visible light (lambda < 420 nm) efficiently generates fluorescent compounds with absorption and emission at 635 nm and 660 nm, respectively. A high-yielding synthetic route with attractive possibilities for further dye design is described in detail. Good photostability, high contrast, and a large fluorescence quantum yield after uncaging are the most important features of the new compounds for non-invasive imaging in high-resolution optical microscopy. For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group."],["dc.identifier.doi","10.1039/c1pp05321c"],["dc.identifier.gro","3142604"],["dc.identifier.isi","000300991600012"],["dc.identifier.pmid","22218703"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Bundesministerium fur Bildung und Forschung (BMBF) [513, FKZ 13N11066]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1474-9092"],["dc.relation.issn","1474-905X"],["dc.title","Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11631"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","11642"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","Sednev, Maksim V."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hebisch, Elke"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:44:44Z"],["dc.date.available","2017-09-07T11:44:44Z"],["dc.date.issued","2016"],["dc.description.abstract","Large Stokes-shift coumarin dyes with an O-phosphorylated 4-(hydroxymethyl)-2,2-dimethyl-1,2,3,4-tetrahydroquinoline fragment emitting in the blue, green, and red regions of the visible spectrum were synthesized. For this purpose, N-substituted and O-protected 1,2-dihydro-7-hydroxy- 2,2,4-trimethylquinoline was oxidized with SeO2 to the corresponding a, alpha,beta-unsaturated aldehyde and then reduced with NaBH4 in a \"one-pot\" fashion to yield N-substituted and 7-O-protected 4-(hydroxymethyl)-7-hydroxy-2,2-dimethyl-1,2,3,4-tetrahydroquinoline as a common precursor to all the coumarin dyes reported here. The photophysical properties of the new dyes (\"reduced coumarins\") and 1,2-dihydroqui-noline analogues (formal precursors) with a trisubstituted C=C bond were compared. The \"reduced coumarins\" were found to be more photoresistant and brighter than their 1,2-dihydroquinoline counterparts. Free carboxylate analogues, as well as their antibody conjugates (obtained from N-hydroxysuccinimidyl esters) were also prepared. All studied conjugates with secondary antibodies afforded high specificity and were suitable for fluorescence microscopy. The red-emitting coumarin dye bearing a betaine fragment at the C-3-position showed excellent performance in stimulation emission depletion (STED) microscopy."],["dc.identifier.doi","10.1002/chem.201601252"],["dc.identifier.gro","3141636"],["dc.identifier.isi","000382921600024"],["dc.identifier.pmid","27385071"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3789"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/148"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [SFB 1002]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A05: Molekulares Imaging von kardialen Calcium-Freisetzungsdomänen"],["dc.relation","SFB 1002 | C02: RhoGTPasen und ihre Bedeutung für die Last-abhängige Myokardfibrose"],["dc.relation.issn","0947-6539"],["dc.relation.issn","1521-3765"],["dc.relation.workinggroup","RG Hell"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.title","\"Reduced\" Coumarin Dyes with an O-Phosphorylated 2,2-Dimethyl-4-( hydroxymethyl)-1,2,3,4-tetrahydroquinoline Fragment: Synthesis, Spectra, and STED Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6266"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","6270"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Kunetsky, R."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:52Z"],["dc.date.available","2017-09-07T11:49:52Z"],["dc.date.issued","2007"],["dc.description.abstract","Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed."],["dc.identifier.doi","10.1002/anie.200702167"],["dc.identifier.gro","3143552"],["dc.identifier.isi","000249114700006"],["dc.identifier.pmid","17640007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1078"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1433-7851"],["dc.title","Photochromic rhodamines provide nanoscopy with optical sectioning"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3290"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","3294"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Mitronova, Gyuzel Yu"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Klocke, Jessica L."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Meineke, Dirk N. H."],["dc.contributor.author","D'Este, E."],["dc.contributor.author","Kraemer, Philip-Tobias"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:38Z"],["dc.date.available","2017-09-07T11:54:38Z"],["dc.date.issued","2016"],["dc.description.abstract","A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of =500-630nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1m solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60nm."],["dc.identifier.doi","10.1002/anie.201511018"],["dc.identifier.gro","3141724"],["dc.identifier.isi","000371418200008"],["dc.identifier.pmid","26844929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/369"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.title","Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","15655"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Angewandte Chemie"],["dc.bibliographiccitation.lastpage","15659"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Roubinet, Benoît"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Alt, Philipp"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","Irie, Masahiro"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:24Z"],["dc.date.available","2018-04-23T11:48:24Z"],["dc.date.issued","2016"],["dc.description.abstract","Reversibel photoschaltbares 1,2‐Bis(2‐ethyl‐6‐phenyl‐1‐benzothiophen‐1,1‐dioxid‐3‐yl)perfluorcyclopenten (EBT) mit fluoreszierender “geschlossener” Form wurde mit vier oder acht Carboxygruppen versehen und an Antikörper gebunden. Die carboxylierten Derivate wiesen geringe Aggregation, effizientes Photoschalten in wässrigen Puffern, gezieltes Färben von zellulären Strukturen und gute photophysikalische Eigenschaften auf. Abwechselnde Bestrahlung mit UV und blauem Licht relativ geringer Intensität führte zu reversibler photochemischer Isomerisierung zwischen zwei stabilen Strukturen über mehrere dutzend Schaltzyklen. Dies ermöglichte die Verwendung der Farbstoffe für hochauflösende RESOLFT‐Mikroskopie (“reversible switchable optical linear fluorescence transitions”). Hierbei konnte eine optische Auflösung von 75 nm an zellulären Tubulin‐Filamenten erzielt werden."],["dc.identifier.doi","10.1002/ange.201607940"],["dc.identifier.gro","3142364"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13503"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0044-8249"],["dc.title","Carboxylierte photoschaltbare Diarylethene als Biomarkierungen für hochauflösende RESOLFT-Mikroskopie"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6070"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Chemistry – A European Journal"],["dc.bibliographiccitation.lastpage","6076"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Grimm, Florian"],["dc.contributor.author","Rehman, Jasmin"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Khan, Taukeer A."],["dc.contributor.author","Schlötel, Jan Gero"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","John, Michael"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2021-06-01T09:41:16Z"],["dc.date.available","2021-06-01T09:41:16Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1002/chem.202005134"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84865"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1521-3765"],["dc.relation.issn","0947-6539"],["dc.title","Rhodamines with a Chloronicotinic Acid Fragment for Live Cell Superresolution STED Microscopy "],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]