Hell, Stefan W.

[["dc.bibliographiccitation.firstpage","550"],["dc.bibliographiccitation.lastpage","560"],["dc.contributor.author","Bewersdorf, Jörg"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.editor","Pawley, James B."],["dc.date.accessioned","2017-09-07T11:53:06Z"],["dc.date.available","2017-09-07T11:53:06Z"],["dc.date.issued","2006"],["dc.description.abstract","Multi-photon processes relying on the cooperative action of two or more photons can broadly be divided into two families that are distinguished by the fact that the photons are either absorbed or scattered (Shen, 1984). Whereas the scattering events relevant to microscopy are second and third harmonic generation (SHG, THG), as well as coherent anti-Stokes Raman scattering (CARS), the useful multi-photon absorption events are two- and threephoton excitation (2PE, 3PE)."],["dc.identifier.doi","10.1007/978-0-387-45524-2_29"],["dc.identifier.gro","3145029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2720"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.publisher","Springer Nature"],["dc.relation.isbn","978-0-387-25921-5"],["dc.relation.ispartof","Handbook Of Biological Confocal Microscopy"],["dc.title","Multifocal Multi-Photon Microscopy"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","404"],["dc.bibliographiccitation.lastpage","413"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.editor","Pawley, James B."],["dc.date.accessioned","2017-09-07T11:53:05Z"],["dc.date.available","2017-09-07T11:53:05Z"],["dc.date.issued","2006"],["dc.description.abstract","Modern optical microscopes are so good that many scientists forget that these instruments only provide their optimal performance if they are used under certain operating conditions. Typical users may be unaware of the very existence of such limitations either because they may unwittingly work within the limits or because they fail to recognize their effects. It is probably also correct to assume that the manufacturer does not intend to discourage purchase by emphasizing the pitfalls that unavoidably arise from the physics of imaging."],["dc.identifier.doi","10.1007/978-0-387-45524-2_20"],["dc.identifier.gro","3145031"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2722"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.publisher","Springer"],["dc.publisher.place","Boston"],["dc.relation.isbn","978-0-387-25921-5"],["dc.relation.ispartof","Handbook Of Biological Confocal Microscopy"],["dc.title","Aberrations in Confocal and Multi-Photon Fluorescence Microscopy Induced by Refractive Index Mismatch"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","561"],["dc.bibliographiccitation.lastpage","570"],["dc.contributor.author","Bewersdorf, Jörg"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.editor","Pawley, James B."],["dc.date.accessioned","2017-09-07T11:53:05Z"],["dc.date.available","2017-09-07T11:53:05Z"],["dc.date.issued","2006"],["dc.description.abstract","The axial (z-) resolution of any fluorescence microscope using a single lens is limited by diffraction to >500nm. While a modest improvement by up to a factor of 2 may be achieved by mathematical deconvolution, a substantial improvement of the axial resolution requires a radical change of the physics of imaging Since the 1990s, two families of methods have evolved that accomplished substantially improved axial resolution in threedimensional (3D) imaging. The first family, comprising 4Pi microscopy and I5M, coherently combines the aperture of two opposing lenses (Hell and Stelzer, 1992a, 1992b; Gustafsson et al., 1995, 1999; Eģner and Hell, 2005). The second family, of which stimulated emission depletion (STED) microscopy (Hell and Wichmann, 1994; see also Chapter 31, this volume) is the most established member, exploits photophysical or photochemical properties of the dye to break the diffraction barrier."],["dc.identifier.doi","10.1007/978-0-387-45524-2_30"],["dc.identifier.gro","3145030"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2721"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.publisher","Springer"],["dc.relation.isbn","978-0-387-25921-5"],["dc.relation.ispartof","Handbook Of Biological Confocal Microscopy"],["dc.title","4Pi Microscopy"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]