Hirrlinger, Johannes

[["dc.bibliographiccitation.firstpage","1229"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","1241"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Rahman, Jamilur"],["dc.contributor.author","Latal, A. Tobias"],["dc.contributor.author","Besser, Stefanie"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T09:26:26Z"],["dc.date.available","2018-11-07T09:26:26Z"],["dc.date.issued","2013"],["dc.description.abstract","Inhibitory neurons are involved in the generation and patterning of the respiratory rhythm in the adult animal. However, the role of glycinergic neurons in the respiratory rhythm in the developing network is still not understood. Although the complete loss of glycinergic transmission in vivo is lethal, the blockade of glycinergic transmission in slices of the medulla has little effect on pre-Botzinger complex network activity. As 50% of the respiratory rhythmic neurons in this slice preparation are glycinergic, they have to be considered as integrated parts of the network. We aimed to investigate whether glycinergic neurons receive mixed miniature inhibitory postsynaptic currents (mIPSCs) that result from co-release of GABA and glycine. Quantification of mixed mIPSCs by the use of different objective detection methods resulted in a wide range of results. Therefore, we generated traces of mIPSCs with a known distribution of mixed mIPSCs and mono-transmitter-induced mIPSCs, and tested the detection methods on the simulated data. We found that analysis paradigms, which are based on fitting the sum of two mIPSC templates, to be most acceptable. On the basis of these protocols, 2040% of all mIPSCs recorded from respiratory glycinergic neurons are mixed mIPSCs that result from co-release of GABA and glycine. Furthermore, single-cell reverse transcriptase polymerase chain reaction revealed that 46% of glycinergic neurons co-express mRNA of glycine transporter 2 together with at least one marker protein of GABAergic neurons. Our data suggest that significant co-transmission occurs in the pre-Botzinger complex that might be involved in the shaping of synaptic inhibition of respiratory glycinergic neurons."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [HI1414/2-1, HU797/7-1]; CMPB"],["dc.identifier.doi","10.1111/ejn.12136"],["dc.identifier.isi","000317850800003"],["dc.identifier.pmid","23347272"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30298"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0953-816X"],["dc.title","Mixed miniature postsynaptic currents resulting from co-release of glycine and GABA recorded from glycinergic neurons in the neonatal respiratory network"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5687"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","The Journal of Physiology"],["dc.bibliographiccitation.lastpage","5705"],["dc.bibliographiccitation.volume","597"],["dc.contributor.author","Gerkau, Niklas J."],["dc.contributor.author","Lerchundi, Rodrigo"],["dc.contributor.author","Nelson, Joel S. E."],["dc.contributor.author","Lantermann, Marina"],["dc.contributor.author","Meyer, Jan"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Rose, Christine R."],["dc.date.accessioned","2021-06-01T10:47:31Z"],["dc.date.available","2021-06-01T10:47:31Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1113/JP278658"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85629"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1469-7793"],["dc.relation.issn","0022-3751"],["dc.title","Relation between activity‐induced intracellular sodium transients and ATP dynamics in mouse hippocampal neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1358"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","1365"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Grass, D."],["dc.contributor.author","Pawlowski, P. G."],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Papadopoulos, Nestoras"],["dc.contributor.author","Richter, Diethelm W."],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Hulsmann, S."],["dc.date.accessioned","2018-11-07T10:51:10Z"],["dc.date.available","2018-11-07T10:51:10Z"],["dc.date.issued","2004"],["dc.description.abstract","A population of neurons in the caudal medulla generates the rhythmic activity underlying breathing movements. Although this neuronal network has attracted great attention for studying neuronal aspects of synaptic transmission, functions of glial cells supporting this neuronal activity remain unclear. To investigate the role of astrocytes in the respiratory network, we applied electrophysiological and immunohistochemical techniques to characterize astrocytes in regions involved in the generation and transmission of rhythmic activity. In the ventral respiratory group and the hypoglossal nucleus (XII) of acutely isolated brainstem slices, we analyzed fluorescently labeled astrocytes obtained from TgN(GFAP-EGFP) transgenic mice with the whole-cell voltage-clamp technique. Three subpopulations of astrocytes could be discerned by their distinct membrane current profiles. A first group of astrocytes was characterized by nonrectifying, symmetrical and voltage-independent potassium currents and a robust glutamate transporter response to D-aspartate. A second group of astrocytes showed additional A-type potassium currents, whereas a third group, identified by immunolabeling for the glial progenitor marker NG2, expressed outwardly rectifying potassium currents, smaller potassium inward currents, and only minimal D-aspartate-induced transporter currents. Astrocytes of all groups showed kainate-induced inward currents. We conclude that most of the astrocytes serve as a buffer system of excess extracellular glutamate and potassium; however, a distinct cell population (NG2-positive, A-type potassium currents) may play an important role for network plasticity."],["dc.identifier.doi","10.1523/JNEUROSCI.4022-03.2004"],["dc.identifier.isi","000188896100012"],["dc.identifier.pmid","14960607"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48825"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.title","Diversity of functional astroglial properties in the respiratory network"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S23"],["dc.bibliographiccitation.journal","NEURON GLIA BIOLOGY"],["dc.bibliographiccitation.lastpage","S24"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Dibaj, Payam"],["dc.contributor.author","Kaiser, M."],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Neusch, C."],["dc.date.accessioned","2018-11-07T11:07:10Z"],["dc.date.available","2018-11-07T11:07:10Z"],["dc.date.issued","2007"],["dc.identifier.isi","000251708800070"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52492"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cambridge Univ Press"],["dc.publisher.place","New york"],["dc.relation.issn","1740-925X"],["dc.title","Co-enrichment of Kir4.1 and AQP4 channels in spinal cord astrocytes suggests coupling of K+ flux and water transport: swelling experiments using transgenic mouse technology and time lapse 2-photon laser microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","EBC20220091"],["dc.bibliographiccitation.journal","Essays in Biochemistry"],["dc.contributor.author","Bohmbach, Kirsten"],["dc.contributor.author","Henneberger, Christian"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.date.accessioned","2022-10-04T10:21:27Z"],["dc.date.available","2022-10-04T10:21:27Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n Learning and memory are fundamental but highly complex functions of the brain. They rely on multiple mechanisms including the processing of sensory information, memory formation, maintenance of short- and long-term memory, memory retrieval and memory extinction. Recent experiments provide strong evidence that, besides neurons, astrocytes crucially contribute to these higher brain functions. However, the complex interplay of astrocytes and neurons in local neuron–glia assemblies is far from being understood. Although important basic cellular principles that govern and link neuronal and astrocytic cellular functions have been established, additional mechanisms clearly continue to emerge. In this short essay, we first review current technologies allowing the experimenter to explore the role of astrocytes in behaving animals, with focus on spatial memory. We then discuss astrocytic signaling mechanisms and their role in learning and memory. We also reveal gaps in our knowledge that currently prevent a comprehensive understanding of how astrocytes contribute to acquisition, storage and retrieval of memory by modulating neuronal signaling in local circuits."],["dc.identifier.doi","10.1042/EBC20220091"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114412"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","1744-1358"],["dc.relation.issn","0071-1365"],["dc.title","Astrocytes in memory formation and maintenance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Oke, Yoshihiko"],["dc.contributor.author","Miwakeichi, Fumikazu"],["dc.contributor.author","Oku, Yoshitaka"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Hülsmann, Swen"],["dc.date.accessioned","2020-12-10T18:44:37Z"],["dc.date.available","2020-12-10T18:44:37Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.3389/fphys.2018.01586"],["dc.identifier.eissn","1664-042X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78533"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.iserratumof","/handle/2/78530"],["dc.title","Corrigendum: Cell Type-Dependent Activation Sequence During Rhythmic Bursting in the PreBötzinger Complex in Respiratory Rhythmic Slices From Mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","erratum_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","97"],["dc.bibliographiccitation.lastpage","100"],["dc.bibliographiccitation.seriesnr","669"],["dc.contributor.author","Winter, Stefan M."],["dc.contributor.author","Fresemann, Jens"],["dc.contributor.author","Schnell, Christian"],["dc.contributor.author","Oku, Yoshitaka"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T08:48:16Z"],["dc.date.available","2018-11-07T08:48:16Z"],["dc.date.issued","2010"],["dc.description.abstract","The neuronal network in the pre-Botzinger Complex is the key element of respiratory rhythm generation. Isolated in a slice preparation, the pre-Botzinger Complex network is still able to generate its inspiratory activity. Although the mechanism of rhythm generation in principle relies on glutamatergic neurons, interestingly we found that glycinergic neurons represent a major portion of all inspiratory neurons in the slice preparation."],["dc.identifier.doi","10.1007/978-1-4419-5692-7_20"],["dc.identifier.isi","000277995200020"],["dc.identifier.pmid","20217329"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21163"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Berlin"],["dc.relation.conference","11th Oxford Conference on Modeling and Control of Breathing"],["dc.relation.crisseries","Advances in Experimental Medicine and Biology"],["dc.relation.eventend","2009-07-26"],["dc.relation.eventlocation","Nara, Japan"],["dc.relation.eventstart","2009-07-23"],["dc.relation.isbn","978-1-4419-5691-0"],["dc.relation.ispartof","New frontiers in respiratory control"],["dc.relation.ispartofseries","Advances in Experimental Medicine and Biology; 669"],["dc.title","Glycinergic Interneurons in the Respiratory Network of the Rhythmic Slice Preparation"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","NEURON GLIA BIOLOGY"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Dibaj, Payam"],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Nadrigny, Fabien"],["dc.contributor.author","Schomburg, Eike D."],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Neusch, Clemens"],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2018-11-07T11:07:11Z"],["dc.date.available","2018-11-07T11:07:11Z"],["dc.date.issued","2007"],["dc.format.extent","S109"],["dc.identifier.isi","000251708800336"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52494"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cambridge Univ Press"],["dc.publisher.place","New york"],["dc.relation.issn","1741-0533"],["dc.relation.issn","1740-925X"],["dc.title","Pharmacological inhibition of the NO-pathway blocks microglia migration following a laser lesion in the mouse spinal cord in vivo"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","173"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Journal of Physiology"],["dc.bibliographiccitation.lastpage","191"],["dc.bibliographiccitation.volume","597"],["dc.contributor.author","Hülsmann, Swen"],["dc.contributor.author","Oke, Yoshihiko"],["dc.contributor.author","Mesuret, Guillaume"],["dc.contributor.author","Latal, A. Tobias"],["dc.contributor.author","Fortuna, Michal G."],["dc.contributor.author","Niebert, Marcus"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Fischer, Julia"],["dc.contributor.author","Hammerschmidt, Kurt"],["dc.date.accessioned","2019-07-30T07:09:26Z"],["dc.date.available","2019-07-30T07:09:26Z"],["dc.date.issued","2019"],["dc.description.abstract","Newborn mice produce ultrasonic vocalization to communicate with their mother. The neuronal glycine transporter (GlyT2) is required for efficient loading of synaptic vesicles in glycinergic neurons. Mice lacking GlyT2 develop a phenotype that resembles human hyperekplexia and the mice die in the second postnatal week. In the present study, we show that GlyT2-knockout mice do not acquire adult ultrasonic vocalization-associated breathing patterns. Despite the strong impairment of glycinergic inhibition, they can produce sufficient expiratory airflow to produce ultrasonic vocalization. Because mouse ultrasonic vocalization is a valuable read-out in translational research, these data are highly relevant for a broad range of research fields."],["dc.identifier.doi","10.1113/JP276976"],["dc.identifier.pmid","30296333"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62159"],["dc.language.iso","en"],["dc.relation.eissn","1469-7793"],["dc.relation.issn","0022-3751"],["dc.relation.issn","1469-7793"],["dc.title","The postnatal development of ultrasonic vocalization-associated breathing is altered in glycine transporter 2-deficient mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1133"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Glia"],["dc.bibliographiccitation.lastpage","1144"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Dibaj, Payam"],["dc.contributor.author","Nadrigny, Fabien"],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Scheller, Anja"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Schomburg, Eike D."],["dc.contributor.author","Neusch, Clemens"],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2018-11-07T08:41:42Z"],["dc.date.available","2018-11-07T08:41:42Z"],["dc.date.issued","2010"],["dc.description.abstract","To understand the pathomechanisms of spinal cord injuries will be a prerequisite to develop efficient therapies. By investigating acute lesions of spinal cord white matter in anesthetized mice with fluorescently labeled microglia and axons using in vivo two-photon laser-scanning microscopy (2P-LSM), we identified the messenger nitric oxide (NO) as a modulator of injury-activated microglia. Local tissue damages evoked by high-power laser pulses provoked an immediate attraction of microglial processes. Spinal superfusion with NO synthase and guanylate cyclase inhibitors blocked these extensions. Furthermore, local injection of the NO-donor spermine NONOate (SPNO) or the NO-dependent second messenger cGMP induced efficient migration of microglial cells toward the injection site. High-tissue levels of NO, achieved by uniform superfusion with SPNO and mimicking extended tissue damage, resulted in a fast conversion of the microglial shape from ramified to ameboid indicating cellular activation. When the spinal white matter was preconditioned by increased, ambient ATP (known as a microglial chemoattractant) levels, the attraction of microglial processes to local NO release was augmented, whereas it was abolished at low levels of tissue ATP. Because both signaling molecules, NO and ATP, mediate acute microglial reactions, coordinated pharmacological targeting of NO and purinergic pathways will be an effective mean to influence the innate immune processes after spinal cord injury. (C) 2010 Wiley-Liss, Inc."],["dc.identifier.doi","10.1002/glia.20993"],["dc.identifier.isi","000278198400011"],["dc.identifier.pmid","20468054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19527"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","0894-1491"],["dc.title","NO Mediates Microglial Response to Acute Spinal Cord Injury Under ATP Control In Vivo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]