Welz, Christian

[["dc.bibliographiccitation.firstpage","2201"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","2210"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Hagemann, Jan"],["dc.contributor.author","Jacobi, Christian"],["dc.contributor.author","Hahn, Moritz"],["dc.contributor.author","Schmid, Vanessa"],["dc.contributor.author","Welz, Christian"],["dc.contributor.author","Schwenk-Zieger, Sabina"],["dc.contributor.author","Stauber, Roland"],["dc.contributor.author","Baumeister, Philipp"],["dc.contributor.author","Becker, Sven"],["dc.date.accessioned","2018-11-07T10:24:11Z"],["dc.date.available","2018-11-07T10:24:11Z"],["dc.date.issued","2017"],["dc.description.abstract","Background/Aim: Chemo-radiation currently serves as first-line therapy of advanced and recurrent head and neck cancer, while new chemotherapy regimens are emerging. However, response rates to any treatment are difficult to predict and underlie broad variation. This study shows the development of a standardized, high-throughput in vitro assay to assess patients' individual response to therapy regimens as a future tool for personalized tumor therapy. Materials and Methods: Viability and proliferation analyses after chemo +/- radiation treatment of single spheroids (low adhesion plates/Hanging Drop (HD)) were generated from head and neck squamous cell carcinoma (HNSCC) cell lines and primary human cells from fresh tumor specimens. Results: All cell lines showed reliable growth in all cell culture methods. The spheroids showed significant delay of growth and/or necrosis compared to control groups when exposed to current standard chemotherapeutic regimens. Single 3D spheroids ready for therapy susceptibility testing could be generated from actual tumor specimens after enzymatic and mechanical separation. Conclusion: In its current form, this single spheroid-based in vitro assay was able to test individual therapy susceptibility to current standard therapy regimens or, potentially, for testing new targeted drugs in HNSCC treatment. With recent discoveries regarding tumor heterogeneity and individual mutation status, a reliable assay is a prerequisite for personalized therapy in head and neck cancer."],["dc.description.sponsorship","Universitat Munchen"],["dc.identifier.doi","10.21873/anticanres.11555"],["dc.identifier.isi","000402173300005"],["dc.identifier.pmid","28476783"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42610"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","1791-7530"],["dc.relation.issn","0250-7005"],["dc.title","Spheroid-based 3D Cell Cultures Enable Personalized Therapy Testing and Drug Discovery in Head and Neck Cancer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0141827"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Welz, Christian"],["dc.contributor.author","Emmert, Steffen"],["dc.contributor.author","Canis, Martin"],["dc.contributor.author","Becker, Sven"],["dc.contributor.author","Baumeister, Philipp"],["dc.contributor.author","Shimizu, Tetsuji"],["dc.contributor.author","Morfill, Gregor E."],["dc.contributor.author","Harreus, Uli"],["dc.contributor.author","Zimmermann, Julia L."],["dc.date.accessioned","2018-11-07T09:48:49Z"],["dc.date.available","2018-11-07T09:48:49Z"],["dc.date.issued","2015"],["dc.description.abstract","Head and neck squamous cell cancer (HNSCC) is the 7th most common cancer worldwide. Despite the development of new therapeutic agents such as monoclonal antibodies, prognosis did not change for the last decades. Cold atmospheric plasma (CAP) presents the most promising new technology in cancer treatment. In this study the efficacy of a surface micro discharging (SMD) plasma device against two head and neck cancer cell lines was proved. Effects on the cell viability, DNA fragmentation and apoptosis induction were evaluated with the MTT assay, alkaline microgel electrophoresis (comet assay) and Annexin-V/PI staining. MTT assay revealed that the CAP treatment markedly decreases the cell viability for all tested treatment times (30, 60, 90, 120 and 180 s). IC 50 was reached within maximal 120 seconds of CAP treatment. Comet assay analysis showed a dose dependent high DNA fragmentation being one of the key players in anti-cancer activity of CAP. Annexin-V/PI staining revealed induction of apoptosis in CAP treated HNSCC cell lines but no significant dose dependency was seen. Thus, we confirmed that SMD Plasma technology is definitely a promising new approach on cancer treatment."],["dc.description.sponsorship","Friedrich-Baur-Stiftung Munich [40/13]"],["dc.identifier.doi","10.1371/journal.pone.0141827"],["dc.identifier.isi","000365251500006"],["dc.identifier.pmid","26588072"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12554"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35387"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Cold Atmospheric Plasma: A Promising Complementary Therapy for Squamous Head and Neck Cancer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","294"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Auris Nasus Larynx"],["dc.bibliographiccitation.lastpage","301"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","Becker, Sven"],["dc.contributor.author","Zimmermann, Julia L."],["dc.contributor.author","Baumeister, Philipp"],["dc.contributor.author","Brunner, Theresa F."],["dc.contributor.author","Shimizu, Tetsuji"],["dc.contributor.author","Li, Yang-Fang"],["dc.contributor.author","Morfill, Gregor E."],["dc.contributor.author","Harréus, Ulrich"],["dc.contributor.author","Welz, Christian"],["dc.date.accessioned","2020-12-10T14:22:24Z"],["dc.date.available","2020-12-10T14:22:24Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.anl.2018.07.008"],["dc.identifier.issn","0385-8146"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71598"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Effects of cold atmospheric plasma (CAP) on bacteria and mucosa of the upper aerodigestive tract"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","343"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Environmental Pathology, Toxicology and Oncology"],["dc.bibliographiccitation.lastpage","354"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Welz, Christian"],["dc.contributor.author","Canis, Martin"],["dc.contributor.author","Schwenk-Zieger, Sabina"],["dc.contributor.author","Becker, Sven"],["dc.contributor.author","Stucke, Vincent"],["dc.contributor.author","Ihler, Friedrich"],["dc.contributor.author","Baumeister, Philipp"],["dc.date.accessioned","2020-12-10T18:42:45Z"],["dc.date.available","2020-12-10T18:42:45Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1615/JEnvironPatholToxicolOncol.v35.i4"],["dc.identifier.issn","0731-8898"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78070"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Cytotoxic and Genotoxic Effects of Electronic Cigarette Liquids on Human Mucosal Tissue Cultures of the Oropharynx"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2432"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Cancers"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Kugler, Philipp"],["dc.contributor.author","Becker, Sven"],["dc.contributor.author","Welz, Christian"],["dc.contributor.author","Wiesmann, Nadine"],["dc.contributor.author","Sax, Jonas"],["dc.contributor.author","Buhr, Christoph R."],["dc.contributor.author","Thoma, Markus H."],["dc.contributor.author","Brieger, Juergen"],["dc.contributor.author","Eckrich, Jonas"],["dc.date.accessioned","2022-06-01T09:39:56Z"],["dc.date.available","2022-06-01T09:39:56Z"],["dc.date.issued","2022"],["dc.description.abstract","Cold atmospheric plasma (CAP) has demonstrated promising anti-cancer effects in numerous in vitro and in vivo studies. Despite their relevance for the treatment of solid tumors, effects of CAP on tumor vasculature and microcirculation have only rarely been investigated. Here, we report the reduction of vessel density and an increase in vascular permeability and tumor cell apoptosis after CAP application. Solid tumors in the chorioallantoic membrane of chicken embryos were treated with CAP and evaluated with respect to effects of CAP on embryo survival, tumor size, and tumor morphology. Furthermore, intratumoral blood vessel density, apoptotic cell death and the tumor-associated microcirculation were investigated and compared to sham treatment. Treatment with CAP significantly reduced intratumoral vessel density while increasing the rate of intratumoral apoptosis in solid tumors. Furthermore, CAP treatment increased vascular permeability and attenuated the microcirculation by causing vessel occlusions in the tumor-associated vasculature. These effects point out the potential of CAP as a promising and yet underrated therapeutic modality for addressing the tumor vasculature in the treatment of solid tumors."],["dc.identifier.doi","10.3390/cancers14102432"],["dc.identifier.pii","cancers14102432"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/108597"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-572"],["dc.relation.eissn","2072-6694"],["dc.title","Cold Atmospheric Plasma Reduces Vessel Density and Increases Vascular Permeability and Apoptotic Cell Death in Solid Tumors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","134"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.contributor.author","Hagemann, Jan"],["dc.contributor.author","Jacobi, Christian"],["dc.contributor.author","Gstoettner, Sabine"],["dc.contributor.author","Welz, Christian"],["dc.contributor.author","Schwenk-Zieger, Sabina"],["dc.contributor.author","Stauber, Roland"],["dc.contributor.author","Strieth, Sebastian"],["dc.contributor.author","Kuenzel, Julian"],["dc.contributor.author","Baumeister, Philipp"],["dc.contributor.author","Becker, Sven"],["dc.date.accessioned","2020-12-10T18:47:29Z"],["dc.date.available","2020-12-10T18:47:29Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.3791/57012"],["dc.identifier.eissn","1940-087X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78782"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]