Ebert, Antje Dagmar

[["dc.bibliographiccitation.artnumber","32669"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Lee, Jaecheol"],["dc.contributor.author","Jung, Seung Min"],["dc.contributor.author","Ebert, Antje D."],["dc.contributor.author","Wu, Haodi"],["dc.contributor.author","Diecke, Sebastian"],["dc.contributor.author","Kim, Youngkyun"],["dc.contributor.author","Yi, Hyoju"],["dc.contributor.author","Park, S. H. P."],["dc.contributor.author","Ju, Ji Hyeon"],["dc.date.accessioned","2018-11-07T10:08:38Z"],["dc.date.available","2018-11-07T10:08:38Z"],["dc.date.issued","2016"],["dc.description.abstract","Cardiovascular disease is a leading cause of morbidity in rheumatoid arthritis (RA) patients. This study aimed to generate and characterise cardiomyocytes from induced pluripotent stem cells (iPSCs) of RA patients. Fibroblast-like synoviocytes (FLSs) from patients with RA and osteoarthritis (OA) were successfully reprogrammed into RA-iPSCs and OA-iPSCs, respectively. The pluripotency of iPSCs was confirmed by quantitative reverse transcription-polymerase chain reaction and immunofluorescence staining. Established iPSCs were differentiated into cardiomyocytes using a small molecule-based monolayer differentiation protocol. Within 12 days of cardiac differentiation from patient-specific and control-iPSCs, spontaneously beating cardiomyocytes (iPSC-CMs) were observed. All iPSC-CMs exhibited a reliable sarcomeric structure stained with antibodies against cardiac markers and similar expression profiles of cardiac-specific genes. Intracellular calcium signalling was recorded to compare calcium-handling properties among cardiomyocytes differentiated from the three groups of iPSCs. RA-iPSC-CMs had a lower amplitude and a shorter duration of calcium transients than the control groups. Peak tangential stress and the maximum contractile rate were also decreased in RA-iPSC-CMs, suggesting that contractility was reduced. This study demonstrates the successful generation of functional cardiomyocytes from pathogenic synovial cells in RA patients through iPSC reprogramming. Research using RA-iPSC-CMs might provide an opportunity to investigate the pathophysiology of cardiac involvement in RA."],["dc.identifier.doi","10.1038/srep32669"],["dc.identifier.isi","000382779200001"],["dc.identifier.pmid","27609119"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13750"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39500"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Generation of Functional Cardiomyocytes from the Synoviocytes of Patients with Rheumatoid Arthritis via Induced Pluripotent Stem Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]