Affenzeller, Susanne

[["dc.bibliographiccitation.artnumber","e0223552"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Affenzeller, Susanne"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Licha, Tobias"],["dc.contributor.author","Jackson, Daniel J."],["dc.contributor.author","Wolkenstein, Klaus"],["dc.date.accessioned","2020-08-04T08:32:28Z"],["dc.date.available","2020-08-04T08:32:28Z"],["dc.date.issued","2019"],["dc.description.abstract","Eumelanin and pheomelanin are well known and common pigments found in nature. However, their complex polymer structure and high thermostability complicate their direct chemical identification. A widely used analytical method is indirect determination using HPLC with UV detection of both types of melanin by their most abundant oxidation products: pyrrole-2,3-dicarboxylic acid (PDCA), pyrrole-2,3,5-tricarboxylic acid (PTCA), thiazole-4,5-dicarboxylic acid (TDCA), and thiazole-2,4,5-tricarboxylic acid (TTCA). An increasing interest in pigmentation in biological research led us to develop a highly sensitive and selective method to identify and quantify these melanin markers in diverse biological samples with complex matrices. By introducing solid-phase extraction (SPE, reversed-phase) following alkaline oxidation we could significantly decrease background signals while maintaining recoveries greater than 70%. Our HPLC-UV-MS method allows for confident peak identification via exact mass information in corresponding UV signals used for quantitation. In addition to synthetic melanin and Sepia officinalis ink as reference compounds eumelanin markers were detected in brown human hair and a brown bivalve shell (Mytilus edulis). Brown feathers from the common chicken (Gallus g. domesticus) yielded all four eumelanin and pheomelanin markers. The present method can be easily adapted for a wide range of future studies on biological samples with unknown melanin content."],["dc.identifier.doi","10.1371/journal.pone.0223552"],["dc.identifier.pmid","31622353"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67513"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Abteilung Geobiologie"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Quantitation of eumelanin and pheomelanin markers in diverse biological samples by HPLC-UV-MS following solid-phase extraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0201396"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Affenzeller, Susanne"],["dc.contributor.author","Cerveau, Nicolas"],["dc.contributor.author","Jackson, Daniel John"],["dc.date.accessioned","2019-07-09T11:46:04Z"],["dc.date.available","2019-07-09T11:46:04Z"],["dc.date.issued","2018"],["dc.description.abstract","Identifying and understanding mechanisms that generate phenotypic diversity is a fundamental goal of evolutionary biology. With a diversity of pigmented shell morphotypes governed by Mendelian patterns of inheritance, the common grove snail Cepaea nemoralis (Linnaeus, 1758) has been a model for evolutionary biologists and population geneticists for decades. However, the genetic mechanisms by which C. nemoralis generates this pigmented shell diversity remain unknown. An important first step in investigating this pigmentation pattern is to establish a set of validated reference genes for differential gene expression assays. Here we have evaluated eleven candidate genes for reverse transcription quantitative polymerase chain reaction (qPCR) in C. nemoralis. Five of these were housekeeping genes traditionally employed as qPCR reference genes in other species, while six alternative genes were selected de novo from C. nemoralis transcriptome data based on the stability of their expression levels. We tested all eleven candidates for expression stability in four sub-adult tissues of C. nemoralis: pigmented mantle, unpigmented mantle, head and foot. We find that two commonly employed housekeeping genes (alpha tubulin, glyceraldehyde 3-phosphate dehydrogenase) are unsuitable for use as qPCR reference genes in C. nemoralis. The traditional housekeeping gene UBIquitin on the other hand performed very well. Additionally, an RNAdirected DNA polymerase (RNAP), a Potassium Channel Protein (KCHP) and a Prenylated Rab acceptor protein 1 (PRAP), identified de novo from transcriptomic data, were the most stably expressed genes in different tissue combinations. We also tested expression stability over two seasons and found that, although other genes are more stable within a single season, beta actin (BACT) and elongation factor 1 alpha (EF1α) were the most reliable reference genes across seasons."],["dc.identifier.doi","10.1371/journal.pone.0201396"],["dc.identifier.pmid","30157182"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15393"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15688"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59376"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","550"],["dc.title","Identification and validation of reference genes for qPCR in the terrestrial gastropod Cepaea nemoralis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","789"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Organisms Diversity & Evolution"],["dc.bibliographiccitation.lastpage","812"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Affenzeller, Susanne"],["dc.contributor.author","Haar, Nicole"],["dc.contributor.author","Steiner, Gerhard"],["dc.date.accessioned","2020-08-04T07:29:29Z"],["dc.date.available","2020-08-04T07:29:29Z"],["dc.date.issued","2017"],["dc.description.abstract","The trochoid genus, Gibbula, is abundant and diverse in the Mediterranean Sea but problematic to identify and delineate. This is due to highly variable shell morphology, vague original descriptions, and missing or unspecific type material. In recent studies, COI barcoding yielded satisfactory results for species delineation. In the present study, a combination of geometric shell morphometric methods and COI barcoding was used to assess the most abundant species of the Eastern Mediterranean. All relevant identification characters were captured via standardised images of the shells in both lateral and ventral views. Agreeing with previous studies, Gibbula was recovered as paraphyletic in the molecular analysis and thus is restricted to the clade encompassing the type species Gibbula magus (Linnaeus, 1758). The geometric morphometric analyses and the barcoding approach clearly distinguish the remaining species into two groups: the genus Steromphala Gray, 1847 and the genus Phorcus Risso, 1826. Type material was used for the geometric morphometric analyses whenever possible. Based on re-examination of the original type descriptions, lectotypes were designated. The joint application of DNA-barcoding and geometric morphometrics not only effectively delineated the sister genera Steromphala and Phorcus but also delineated all analysed species in the Gibbula-Steromphala-Phorcus genus complex. The additional use of geometric morphometrics enables researchers to compare barcoded material with fossil specimens or dry collections in an objective way."],["dc.identifier.doi","10.1007/s13127-017-0343-5"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67510"],["dc.language.iso","en"],["dc.relation.eissn","1618-1077"],["dc.relation.issn","1439-6092"],["dc.title","Revision of the genus complex Gibbula: an integrative approach to delineating the Eastern Mediterranean genera Gibbula Risso, 1826, Steromphala Gray, 1847, and Phorcus Risso, 1826 using DNA-barcoding and geometric morphometrics (Vetigastropoda, Trochoidea)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2442"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Affenzeller, Susanne"],["dc.contributor.author","Wolkenstein, Klaus"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Jackson, Daniel J."],["dc.date.accessioned","2020-08-04T07:37:40Z"],["dc.date.available","2020-08-04T07:37:40Z"],["dc.date.issued","2020"],["dc.description.abstract","The common grove snail Cepaea nemoralis displays a stable pigmentation polymorphism in its shell that has held the attention of scientists for decades. While the details of the molecular mechanisms that generate and maintain this diversity remain elusive, it has long been employed as a model system to address questions related to ecology, population genetics and evolution. In order to contribute to the ongoing efforts to identify the genes that generate this polymorphism we have tested the long-standing assumption that melanin is the pigment that comprises the dark-brown bands. Surprisingly, using a newly established analytical chemical method, we find no evidence that eumelanin is differentially distributed within the shells of C. nemoralis. Furthermore, genes known to be responsible for melanin deposition in other metazoans are not differentially expressed within the shell-forming mantle tissue of C. nemoralis. These results have implications for the continuing search for the supergene that generates the various pigmentation morphotypes."],["dc.identifier.doi","10.1038/s41598-020-59185-y"],["dc.identifier.pmid","32051478"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67511"],["dc.language.iso","en"],["dc.relation.issn","2045-2322"],["dc.relation.orgunit","Abteilung Geobiologie"],["dc.title","Challenging the concept that eumelanin is the polymorphic brown banded pigment in Cepaea nemoralis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","73"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Zootaxa"],["dc.bibliographiccitation.lastpage","90"],["dc.bibliographiccitation.volume","4337"],["dc.contributor.author","Affenzeller, Susanne"],["dc.contributor.author","Steiner, Gerhard"],["dc.date.accessioned","2020-08-04T07:28:23Z"],["dc.date.available","2020-08-04T07:28:23Z"],["dc.date.issued","2017-10-17"],["dc.description.abstract","Luitfried Salvini-Plawen was one of the most distinguished researchers for molluscan phylogenetic systematics of the last decades. In his publications he described a total of 193 species: 134 Solenogastres, 34 Caudofoveata, 14 interstitial Gastropoda, one polyplacophoran and the remaining comprising Cnidaria, Priapulida, Kamptozoa, and Echinodermata. In addition, he introduced 47 genus-group names and 54 names for family-level and higher taxa. This catalog comprises lists of all taxon names published by Luitfried Salvini-Plawen. The catalog entries contain taxonomic information, original citations, type localities and type collections. It aims to facilitate further research on these and related taxa."],["dc.identifier.doi","10.11646/zootaxa.4337.1.3"],["dc.identifier.pmid","29242431"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67509"],["dc.language.iso","en"],["dc.relation.eissn","1175-5326"],["dc.relation.issn","1175-5334"],["dc.title","Catalog of taxa introduced by Luitfried Salvini-Plawen (1939-2014)"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","47"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Frontiers in Zoology"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Affenzeller, Susanne"],["dc.contributor.author","Wolkenstein, Klaus"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Jackson, Daniel J."],["dc.date.accessioned","2020-08-04T07:39:30Z"],["dc.date.available","2020-08-04T07:39:30Z"],["dc.date.issued","2019"],["dc.description.abstract","The geometric patterns that adorn the shells of many phylogenetically disparate molluscan species are comprised of pigments that span the visible spectrum. Although early chemical studies implicated melanin as a commonly employed pigment, surprisingly little evidence generated with more recent and sensitive techniques exists to support these observations."],["dc.identifier.doi","10.1186/s12983-019-0346-5"],["dc.identifier.pmid","31889966"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17046"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67512"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1742-9994"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Eumelanin and pheomelanin pigmentation in mollusc shells may be less common than expected: insights from mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]