Wemheuer, Bernd

[["dc.bibliographiccitation.firstpage","13"],["dc.bibliographiccitation.lastpage","21"],["dc.bibliographiccitation.seriesnr","2555"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Schneider, Dominik"],["dc.contributor.author","Pfeiffer, Birgit"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.editor","Streit, Wolfgang R."],["dc.contributor.editor","Daniel, Rolf"],["dc.date.accessioned","2022-11-01T10:17:28Z"],["dc.date.available","2022-11-01T10:17:28Z"],["dc.date.issued","2023"],["dc.identifier.doi","10.1007/978-1-0716-2795-2_2"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116819"],["dc.notes.intern","DOI-Import GROB-605"],["dc.publisher","Springer US"],["dc.publisher.place","New York, NY"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.eisbn","978-1-0716-2795-2"],["dc.relation.isbn","978-1-0716-2794-5"],["dc.relation.ispartof","Metagenomics : Methods and Protocols"],["dc.title","Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","13"],["dc.bibliographiccitation.lastpage","22"],["dc.bibliographiccitation.volume","1539"],["dc.contributor.author","Schneider, Dominik"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Pfeiffer, Birgit"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.editor","Streit, W."],["dc.contributor.editor","Daniel, R."],["dc.date.accessioned","2020-03-12T09:01:08Z"],["dc.date.available","2020-03-12T09:01:08Z"],["dc.date.issued","2017"],["dc.description.abstract","Microbial communities play an important role in marine ecosystem processes. Although the number of studies targeting marker genes such as the 16S rRNA gene has been increased in the last few years, the vast majority of marine diversity is rather unexplored. Moreover, most studies focused on the entire bacterial community and thus disregarded active microbial community players. Here, we describe a detailed protocol for the simultaneous extraction of DNA and RNA from marine water samples and for the generation of cDNA from the isolated RNA which can be used as a universal template in various marker gene studies."],["dc.identifier.doi","10.1007/978-1-4939-6691-2_2"],["dc.identifier.pmid","27900681"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63326"],["dc.language.iso","en"],["dc.publisher","Humana Press"],["dc.publisher.place","New York, NY"],["dc.relation.eisbn","978-1-4939-6691-2"],["dc.relation.eissn","1940-6029"],["dc.relation.isbn","978-1-4939-6689-9"],["dc.relation.ispartof","Metagenomics. Methods in Molecular Biology"],["dc.relation.issn","1064-3745"],["dc.title","Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a cDNA as Universal Template for Marker Gene Studies"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","75"],["dc.bibliographiccitation.lastpage","84"],["dc.bibliographiccitation.volume","1539"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.editor","Streit, W."],["dc.contributor.editor","Daniel, R."],["dc.date.accessioned","2020-04-28T12:42:00Z"],["dc.date.available","2020-04-28T12:42:00Z"],["dc.date.issued","2017"],["dc.description.abstract","Plants are colonized various microorganisms including endophytes. These microbes can play an important role in agricultural production as they promote plant growth and/or enhance the resistance of their host plant against diseases and environmental stress conditions. Although culture-independent molecular approaches such as DNA barcoding have greatly enhanced our understanding of bacterial and fungal endophyte communities, there are some methodical problems when investigating endophyte diversity. One main issue are sequence contaminations such as plastid-derived rRNA gene sequences which are co-amplified due to their high homology to bacterial 16S rRNA genes. The same is true for plant and fungal ITS sequences. The application of highly specific-primers suppressing co-amplification of these sequence contaminations is a good solution for this issue. Here, we describe a detailed protocol for assessing bacterial and fungal endophyte diversity in plants using these primers in combination with next-generation sequencing."],["dc.identifier.doi","10.1007/978-1-4939-6691-2_6"],["dc.identifier.pmid","27900685"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64452"],["dc.language.iso","en"],["dc.publisher","Humana Press"],["dc.publisher.place","New York, NY"],["dc.relation.eisbn","978-1-4939-6691-2"],["dc.relation.isbn","978-1-4939-6689-9"],["dc.relation.ispartof","Metagenomics. Methods in Molecular Biology"],["dc.title","Assessing Bacterial and Fungal Diversity in the Plant Endosphere"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]