Schilbach, Sandra

[["dc.bibliographiccitation.journal","Nature Structural & Molecular Biology"],["dc.contributor.author","Rengachari, Srinivasan"],["dc.contributor.author","Schilbach, Sandra"],["dc.contributor.author","Kaliyappan, Thangavelu"],["dc.contributor.author","Gouge, Jerome"],["dc.contributor.author","Zumer, Kristina"],["dc.contributor.author","Schwarz, Juliane"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Dienemann, Christian"],["dc.contributor.author","Vannini, Alessandro"],["dc.contributor.author","Cramer, Patrick"],["dc.date.accessioned","2022-12-01T08:30:54Z"],["dc.date.available","2022-12-01T08:30:54Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes has been studied in detail, Pol II initiation at non-coding genes, such as small nuclear RNA (snRNA) genes, is less well understood at the structural level. Here, we study Pol II initiation at snRNA gene promoters and show that the snRNA-activating protein complex (SNAPc) enables DNA opening and transcription initiation independent of TFIIE and TFIIH in vitro. We then resolve cryo-EM structures of the SNAPc-containing Pol IIpre-initiation complex (PIC) assembled on U1 and U5 snRNA promoters. The core of SNAPc binds two turns of DNA and recognizes the snRNA promoter-specific proximal sequence element (PSE), located upstream of the TATA box-binding protein TBP. Two extensions of SNAPc, called wing-1 and wing-2, bind TFIIA and TFIIB, respectively, explaining how SNAPc directs Pol II to snRNA promoters. Comparison of structures of closed and open promoter complexes elucidates TFIIH-independent DNA opening. These results provide the structural basis of Pol II initiation at non-coding RNA gene promoters."],["dc.identifier.doi","10.1038/s41594-022-00857-w"],["dc.identifier.pii","857"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118010"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1545-9985"],["dc.relation.issn","1545-9993"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Structural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","97"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Cell"],["dc.bibliographiccitation.lastpage","106.e4"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Dienemann, Christian"],["dc.contributor.author","Schwalb, Björn"],["dc.contributor.author","Schilbach, Sandra"],["dc.contributor.author","Cramer, Patrick"],["dc.date.accessioned","2022-03-01T11:45:17Z"],["dc.date.available","2022-03-01T11:45:17Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.molcel.2018.10.014"],["dc.identifier.pii","S1097276518308463"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103276"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","1097-2765"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Promoter Distortion and Opening in the RNA Polymerase II Cleft"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","204"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","209"],["dc.bibliographiccitation.volume","551"],["dc.contributor.author","Schilbach, S."],["dc.contributor.author","Hantsche, M."],["dc.contributor.author","Tegunov, D."],["dc.contributor.author","Dienemann, C."],["dc.contributor.author","Wigge, C."],["dc.contributor.author","Urlaub, H."],["dc.contributor.author","Cramer, P."],["dc.date.accessioned","2018-01-09T12:04:30Z"],["dc.date.available","2018-01-09T12:04:30Z"],["dc.date.issued","2017"],["dc.description.abstract","For the initiation of transcription, RNA polymerase II (Pol II) assembles with general transcription factors on promoter DNA to form the pre-initiation complex (PIC). Here we report cryo-electron microscopy structures of the Saccharomyces cerevisiae PIC and PIC-core Mediator complex at nominal resolutions of 4.7 Å and 5.8 Å, respectively. The structures reveal transcription factor IIH (TFIIH), and suggest how the core and kinase TFIIH modules function in the opening of promoter DNA and the phosphorylation of Pol II, respectively. The TFIIH core subunit Ssl2 (a homologue of human XPB) is positioned on downstream DNA by the 'E-bridge' helix in TFIIE, consistent with TFIIE-stimulated DNA opening. The TFIIH kinase module subunit Tfb3 (MAT1 in human) anchors the kinase Kin28 (CDK7), which is mobile in the PIC but preferentially located between the Mediator hook and shoulder in the PIC-core Mediator complex. Open spaces between the Mediator head and middle modules may allow access of the kinase to its substrate, the C-terminal domain of Pol II."],["dc.identifier.doi","10.1038/nature24282"],["dc.identifier.pmid","29088706"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11583"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1476-4687"],["dc.title","Structures of transcription pre-initiation complex with TFIIH and Mediator"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]