Luther, Stefan

[["dc.bibliographiccitation.firstpage","4832"],["dc.bibliographiccitation.lastpage","4835"],["dc.bibliographiccitation.volume","2018"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Schwaerzle, Michael"],["dc.contributor.author","Ruther, Patrick"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Richter, Claudia"],["dc.date.accessioned","2022-05-16T13:44:04Z"],["dc.date.available","2022-05-16T13:44:04Z"],["dc.date.issued","2018-07"],["dc.description.abstract","One major cause of death in the industrialized world is sudden cardiac death, which so far can be reliably treated only by applying strong electrical shocks. Developing improved methods, aiming at lowering shock intensity and associated side effects potentially has significant clinical implications. Thus, optogenetic stimulation using structured illumination has been introduced as a promising experimental tool to investigate mechanisms underlying multi-site pacing and to optimize potential low-energy approaches. Furthermore, an objective of this work is to strengthen the application of optogenetic tools for cardiac arrhythmia research, which in turn is expected to improve applicable technologies towards tissue-protective defibrillation."],["dc.identifier.doi","10.1109/EMBC.2018.8513124"],["dc.identifier.pmid","30441427"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107982"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/292"],["dc.language.iso","en"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation.conference","40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)"],["dc.relation.eventend","2018-07-21"],["dc.relation.eventlocation","Honolulu, HI"],["dc.relation.eventstart","2018-07-18"],["dc.relation.isbn","978-1-5386-3646-6"],["dc.relation.ispartof","Proceedings"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Follow the Light - From Low-Energy Defibrillation to Multi-Site Photostimulation"],["dc.type","conference_paper"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1651"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Quiñonez Uribe, Raúl A."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Richter, Claudia"],["dc.date.accessioned","2019-07-09T11:50:14Z"],["dc.date.available","2019-07-09T11:50:14Z"],["dc.date.issued","2018"],["dc.description.abstract","Complex spatiotemporal non-linearity as observed during cardiac arrhythmia strongly correlates with vortex-like excitation wavelengths and tissue characteristics. Therefore, the control of arrhythmic patterns requires fundamental understanding of dependencies between onset and perpetuation of arrhythmia and substrate instabilities. Available treatments, such as drug application or high-energy electrical shocks, are discussed for potential side effects resulting in prognosis worsening due to the lack of specificity and spatiotemporal precision. In contrast, cardiac optogenetics relies on light sensitive ion channels stimulated to trigger excitation of cardiomyocytes solely making use of the inner cell mechanisms. This enables low-energy, non-damaging optical control of cardiac excitation with high resolution. Recently, the capability of optogenetic cardioversion was shown in Channelrhodopsin-2 (ChR2) transgenic mice. But these studies used mainly structured and local illumination for cardiac stimulation. In addition, since optogenetic and electrical stimulus work on different principles to control the electrical activity of cardiac tissue, a better understanding of the phenomena behind optogenetic cardioversion is still needed. The present study aims to investigate global illumination with regard to parameter characterization and its potential for cardioversion. Our results show that by tuning the light intensity without exceeding 1.10 mW mm-2, a single pulse in the range of 10-1,000 ms is sufficient to reliably reset the heart into sinus rhythm. The combination of our panoramic low-intensity photostimulation with optical mapping techniques visualized wave collision resulting in annihilation as well as propagation perturbations as mechanisms leading to optogenetic cardioversion, which seem to base on other processes than electrical defibrillation. This study contributes to the understanding of the roles played by epicardial illumination, pulse duration and light intensity in optogenetic cardioversion, which are the main variables influencing cardiac optogenetic control, highlighting the advantages and insights of global stimulation. Therefore, the presented results can be modules in the design of novel illumination technologies with specific energy requirements on the way toward tissue-protective defibrillation techniques."],["dc.identifier.doi","10.3389/fphys.2018.01651"],["dc.identifier.pmid","30542292"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15889"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59730"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/302"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/241526/EU//EUTRIGTREAT"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation.eissn","1664-042X"],["dc.relation.issn","1664-042X"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","610"],["dc.title","Energy-Reduced Arrhythmia Termination Using Global Photostimulation in Optogenetic Murine Hearts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","174"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Steinebach, Janna"],["dc.contributor.author","Schwaerzle, Michael"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Ruther, Patrick"],["dc.contributor.author","Richter, Claudia"],["dc.date.accessioned","2021-12-01T09:23:13Z"],["dc.date.available","2021-12-01T09:23:13Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.3791/62335"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94593"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","1940-087X"],["dc.title","Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","104820G"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.seriesnr","1048219"],["dc.contributor.author","Diaz-Maue, Laura"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Richter, Claudia"],["dc.contributor.editor","Mohanty, Samarendra K."],["dc.contributor.editor","Thakor, Nitish V."],["dc.contributor.editor","Jansen, E. Duco"],["dc.date.accessioned","2019-02-27T16:47:31Z"],["dc.date.available","2019-02-27T16:47:31Z"],["dc.date.issued","2018"],["dc.description.abstract","Detailed understanding of mechanisms and instabilities underlying the onset, perpetuation, and control of cardiac arrhythmias is required for the development, further optimization, and translation of clinically applicable defibrillation methods. Recently, the potential use of optogenetic tools using structured illumination to control cardiac arrhythmia has been successfully demonstrated and photostimulation turned out to be a promising experimental tool to investigate the dynamics and mechanisms of multi-site pacing strategies for low-energy defibrillation. In order to study the relation between trigger and control mechanisms of arrhythmic cardiac conditions without external affecting factors like eventually damaging fiber poking, it is important to establish a non-invasive photostimulation method. Hence, we applied a custom-configured digital light processing micromirror array operated by a high-speed FPGA, which guarantees a high frequency control of stimulation patterns. The integration into a highly sophisticated optical experiment setup allows us to record photostimulation effects and to proof the light pulse as origin of cardiac excitation. Experiments with transgenic murine hearts demonstrate the successful induction and termination of cardiac dysrhythmia using light crafting tools. However, the complex spatiotemporal dynamics underlying arrhythmia critically depends on the ratio of the characteristic wavelength of arrhythmia and substrate size. Based on the experimental evidence regarding the feasibility of optical defibrillation in small mammals, the transfer in clinically relevant large animal models would be the next milestone to therapeutic translation. Thus, the presented experimental results of optogenetically modified murine hearts function as originator for ongoing studies involving principle design studies for therapeutic applicable optical defibrillation."],["dc.identifier.doi","10.1117/12.2288976"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57656"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/207"],["dc.language.iso","en"],["dc.notes.status","fcwi"],["dc.publisher","SPIE"],["dc.publisher.place","Bellingham, Washington"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation.conference","SPIE BiOS"],["dc.relation.crisseries","Proceedings of SPIE"],["dc.relation.eventend","2018-01-28"],["dc.relation.eventlocation","San Francisco"],["dc.relation.eventstart","2018-01-27"],["dc.relation.isbn","978-1-5106-1449-9"],["dc.relation.isbn","978-1-5106-1450-5"],["dc.relation.ispartof","Optogenetics and Optical Manipulation 2018"],["dc.relation.ispartofseries","Proceedings of SPIE; 1048219"],["dc.relation.issn","1605-7422"],["dc.relation.issn","1996-756X"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Towards optogenetic control of spatiotemporal cardiac dynamics"],["dc.type","conference_paper"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]