Thomssen, Reiner

[["dc.bibliographiccitation.firstpage","177"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Medical Microbiology and Immunology"],["dc.bibliographiccitation.lastpage","184"],["dc.bibliographiccitation.volume","188"],["dc.contributor.author","Monazahian, M."],["dc.contributor.author","Kippenberger, S."],["dc.contributor.author","Mueller, A."],["dc.contributor.author","Seitz, H."],["dc.contributor.author","Bohme, I."],["dc.contributor.author","Grethe, S."],["dc.contributor.author","Thomssen, R."],["dc.date.accessioned","2018-11-07T10:48:52Z"],["dc.date.available","2018-11-07T10:48:52Z"],["dc.date.issued","2000"],["dc.description.abstract","Heterogeneities in the density of hepatitis C virus (HCV)-RNA-carrying material from human sera (1.03-1.20 g/ml) are partially due to the binding of lipoproteins [low density (LDL), very low density (VLDL), high density (HDL) lipoproteins] and immunoglobulins. In this study we demonstrate the binding of recombinant HCV envelope protein (E1/E2) to human LDL, VLDL and HDL on a molecular basis. The binding: of lipoproteins was restricted to the middle part of the El gene product (amino acids 222-336) and the C-terminal part of the E2 protein (amino acids 523-809). Lipoproteins did not bind to recombinant HCV core protein."],["dc.identifier.doi","10.1007/s004300000032"],["dc.identifier.isi","000088186500003"],["dc.identifier.pmid","10917154"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48300"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0300-8584"],["dc.title","Binding of human lipoproteins (low, very low, high density lipoproteins) to recombinant envelope proteins of hepatitis C virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","152"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Medical Virology"],["dc.bibliographiccitation.lastpage","158"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Grethe, S."],["dc.contributor.author","Gemsa, F."],["dc.contributor.author","Monazahian, M."],["dc.contributor.author","Bohme, I."],["dc.contributor.author","Uy, Angela"],["dc.contributor.author","Thomssen, R."],["dc.date.accessioned","2018-11-07T10:36:39Z"],["dc.date.available","2018-11-07T10:36:39Z"],["dc.date.issued","2000"],["dc.description.abstract","Infection with hepatitis C virus (HCV) is still a serious problem in hemodialysis patients, despite screening of blood products for anti-HCV antibodies. The prevalence of HCV in HD patients is between 15% and 30% in Germany. We report the molecular epidemiology of an HCV outbreak in a hemodialysis unit in 1997 is determined. HCV hypervariable region 1 (HVR1) was amplified from serum samples of 19 patients by polymerase chain reaction (PCR) and sequenced directly. In addition, HCV isolates from 3 of these 19 patients were cloned and sequenced. 14 newly infected patients and two patients, who had been infected for several years had very closely related HCV isolates. Unrelated HCV isolates as well as sequences obtained from an HCV outbreak in a plasmapheresis center were found in different, distantly related branches. These findings provide strong evidence for nosocomial transmission of the virus, despite following strict general hygiene precautions. The production of anti-HCV antibody was delayed significantly or seroconversion did not occur at all during the period of: observation in 8 out of 14 newly infected HCV RNA positive patients. Close-meshed reverse transcription-polymerase chain reaction (RT-PCR) analyses on apparently non infected patients within hemodialysis units and upon admission of new patients is strongly recommended for the early detection and prevention of outbreaks of HCV. J. Med. Virol, 60:152-158, 2000. (C) 2000 Wiley-Liss, Inc."],["dc.identifier.doi","10.1002/(SICI)1096-9071(200002)60:2<152::AID-JMV8>3.0.CO;2-I"],["dc.identifier.isi","000084428300008"],["dc.identifier.pmid","10596014"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45380"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","0146-6615"],["dc.title","Molecular epidemiology of an outbreak of HCV in a hemodialysis unit: Direct sequencing of HCV-HVR1 as an appropriate tool for phylogenetic analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]