Pöhlmann, Stefan

[["dc.bibliographiccitation.artnumber","e0212757"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PlOS ONE"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Bdeir, Najat"],["dc.contributor.author","Arora, Prerna"],["dc.contributor.author","Gärtner, Sabine"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Reichl, Udo"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Winkler, Michael"],["dc.date.accessioned","2019-07-09T11:50:20Z"],["dc.date.available","2019-07-09T11:50:20Z"],["dc.date.issued","2019"],["dc.description.abstract","Influenza A virus (IAV) infection poses a serious health threat and novel antiviral strategies are needed. Defective interfering particles (DIPs) can be generated in IAV infected cells due to errors of the viral polymerase and may suppress spread of wild type (wt) virus. The antiviral activity of DIPs is exerted by a DI genomic RNA segment that usually contains a large deletion and suppresses amplification of wt segments, potentially by competing for cellular and viral resources. DI-244 is a naturally occurring prototypic segment 1-derived DI RNA in which most of the PB2 open reading frame has been deleted and which is currently developed for antiviral therapy. At present, coinfection with wt virus is required for production of DI-244 particles which raises concerns regarding biosafety and may complicate interpretation of research results. Here, we show that cocultures of 293T and MDCK cell lines stably expressing codon optimized PB2 allow production of DI-244 particles solely from plasmids and in the absence of helper virus. Moreover, we demonstrate that infectivity of these particles can be quantified using MDCK-PB2 cells. Finally, we report that the DI-244 particles produced in this novel system exert potent antiviral activity against H1N1 and H3N2 IAV but not against the unrelated vesicular stomatitis virus. This is the first report of DIP production in the absence of infectious IAV and may spur efforts to develop DIPs for antiviral therapy."],["dc.identifier.doi","10.1371/journal.pone.0212757"],["dc.identifier.pmid","30822349"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15912"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59749"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","599"],["dc.title","A system for production of defective interfering particles in the absence of infectious influenza A virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14057"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","23"],["dc.contributor.affiliation","Arora, Prerna; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.affiliation","Zhang, Lu; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.affiliation","Rocha, Cheila; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.affiliation","Graichen, Luise; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.affiliation","Nehlmeier, Inga; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.affiliation","Kempf, Amy; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.affiliation","Cossmann, Anne; 3Department for Rheumatology and Immunology, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany"],["dc.contributor.affiliation","Ramos, Gema Morillas; 3Department for Rheumatology and Immunology, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany"],["dc.contributor.affiliation","Baier, Eva; 5Department of Nephrology and Rheumatology, University Medical Center Göttingen, Georg-August University of Göttingen, Robert-Koch-Straße 40, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Tampe, Björn; 5Department of Nephrology and Rheumatology, University Medical Center Göttingen, Georg-August University of Göttingen, Robert-Koch-Straße 40, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Moerer, Onnen; 6Department of Anesthesiology, University Medical Center Göttingen, Georg-August University of Göttingen, Robert-Koch-Straße 40, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Dickel, Steffen; 6Department of Anesthesiology, University Medical Center Göttingen, Georg-August University of Göttingen, Robert-Koch-Straße 40, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Winkler, Martin S.; 6Department of Anesthesiology, University Medical Center Göttingen, Georg-August University of Göttingen, Robert-Koch-Straße 40, 37075 Göttingen, Germany"],["dc.contributor.affiliation","Behrens, Georg M. N.; 3Department for Rheumatology and Immunology, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany"],["dc.contributor.affiliation","Pöhlmann, Stefan; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.affiliation","Hoffmann, Markus; 1Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany"],["dc.contributor.author","Arora, Prerna"],["dc.contributor.author","Zhang, Lu"],["dc.contributor.author","Rocha, Cheila"],["dc.contributor.author","Graichen, Luise"],["dc.contributor.author","Nehlmeier, Inga"],["dc.contributor.author","Kempf, Amy"],["dc.contributor.author","Cossmann, Anne"],["dc.contributor.author","Ramos, Gema Morillas"],["dc.contributor.author","Baier, Eva"],["dc.contributor.author","Tampe, Björn"],["dc.contributor.author","Moerer, Onnen"],["dc.contributor.author","Dickel, Steffen"],["dc.contributor.author","Winkler, Martin S."],["dc.contributor.author","Behrens, Georg M. N."],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Hoffmann, Markus"],["dc.date.accessioned","2022-12-07T15:52:24Z"],["dc.date.available","2022-12-07T15:52:24Z"],["dc.date.issued","2022-11-14"],["dc.date.updated","2022-12-07T13:05:30Z"],["dc.description.abstract","Recently, a recombinant SARS-CoV-2 lineage, XD, emerged that harbors a spike gene that is largely derived from the Omicron variant BA.1 in the genetic background of the Delta variant. This finding raised concerns that the recombinant virus might exhibit altered biological properties as compared to the parental viruses and might pose an elevated threat to human health. Here, using pseudotyped particles, we show that ACE2 binding and cell tropism of XD mimics that of BA.1. Further, XD and BA.1 displayed comparable sensitivity to neutralization by antibodies induced upon vaccination with BNT162b2/Comirnaty (BNT) or BNT vaccination followed by breakthrough infection. Our findings reveal important biological commonalities between XD and Omicron BA.1 host cell entry and its inhibition by antibodies."],["dc.description.sponsorship","German Federal Ministry of Education and Research (Bundesministerium für Bildung und Forschung)"],["dc.description.sponsorship","Ministry for Science and Culture of Lower Saxony (Niedersächsisches Ministerium für Wissenschaft und Kultur)"],["dc.description.sponsorship","German Research Foundation (Deutsche Forschungsgemeinschaft)"],["dc.description.sponsorship","German Center for Infection Research"],["dc.description.sponsorship","European Commission via the EU UNDINE project"],["dc.description.sponsorship","European Regional Development Fund"],["dc.description.sponsorship","Sartorius AG (unrestricted funding, MSW)"],["dc.identifier.doi","10.3390/ijms232214057"],["dc.identifier.pii","ijms232214057"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118476"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1422-0067"],["dc.rights","CC BY 4.0"],["dc.title","The SARS-CoV-2 Delta-Omicron Recombinant Lineage (XD) Exhibits Immune-Escape Properties Similar to the Omicron (BA.1) Variant"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]