Pöhlmann, Stefan

[["dc.bibliographiccitation.artnumber","e43337"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Winkler, Michael"],["dc.contributor.author","Bertram, Stephanie"],["dc.contributor.author","Gnirß, Kerstin"],["dc.contributor.author","Nehlmeier, Inga"],["dc.contributor.author","Gawanbacht, Ali"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Ehrhardt, Christina"],["dc.contributor.author","Ludwig, Stephan"],["dc.contributor.author","Kiene, Miriam"],["dc.contributor.author","Moldenhauer, Anna-Sophie"],["dc.contributor.author","Goedecke, Ulrike"],["dc.contributor.author","Karsten, Christina B."],["dc.contributor.author","Kühl, Annika"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2019-07-09T11:53:44Z"],["dc.date.available","2019-07-09T11:53:44Z"],["dc.date.issued","2012"],["dc.description.abstract","The interferon-induced host cell factor tetherin inhibits release of human immunodeficiency virus (HIV) from the plasma membrane of infected cells and is counteracted by the HIV-1 protein Vpu. Influenza A virus (FLUAV) also buds from the plasma membrane and is not inhibited by tetherin. Here, we investigated if FLUAV encodes a functional equivalent of Vpu for tetherin antagonism. We found that expression of the FLUAV protein NS1, which antagonizes the interferon (IFN) response, did not block the tetherin-mediated restriction of HIV release, which was rescued by Vpu. Similarly, tetherinmediated inhibition of HIV release was not rescued by FLUAV infection. In contrast, FLUAV infection induced tetherin expression on target cells in an IFN-dependent manner. These results suggest that FLUAV escapes the antiviral effects of tetherin without encoding a tetherin antagonist with Vpu-like activity."],["dc.identifier.doi","10.1371/journal.pone.0043337"],["dc.identifier.fs","588509"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7923"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60484"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Influenza A Virus Does Not Encode a Tetherin Antagonist with Vpu-Like Activity and Induces IFN-Dependent Tetherin Expression in Infected Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e35876"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Bertram, Stephanie"],["dc.contributor.author","Heurich, Adeline"],["dc.contributor.author","Lavender, Hayley"],["dc.contributor.author","Gierer, Stefanie"],["dc.contributor.author","Danisch, Simon"],["dc.contributor.author","Perin, Paula"],["dc.contributor.author","Lucas, Jared M."],["dc.contributor.author","Nelson, Peter S."],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Soilleux, Elizabeth J."],["dc.date.accessioned","2019-07-09T11:53:44Z"],["dc.date.available","2019-07-09T11:53:44Z"],["dc.date.issued","2012"],["dc.description.abstract","The type II transmembrane serine proteases TMPRSS2 and HAT activate influenza viruses and the SARS-coronavirus (TMPRSS2) in cell culture and may play an important role in viral spread and pathogenesis in the infected host. However, it is at present largely unclear to what extent these proteases are expressed in viral target cells in human tissues. Here, we show that both HAT and TMPRSS2 are coexpressed with 2,6-linked sialic acids, the major receptor determinant of human influenza viruses, throughout the human respiratory tract. Similarly, coexpression of ACE2, the SARS-coronavirus receptor, and TMPRSS2 was frequently found in the upper and lower aerodigestive tract, with the exception of the vocal folds, epiglottis and trachea. Finally, activation of influenza virus was conserved between human, avian and porcine TMPRSS2, suggesting that this protease might activate influenza virus in reservoir-, intermediate- and human hosts. In sum, our results show that TMPRSS2 and HAT are expressed by important influenza and SARS-coronavirus target cells and could thus support viral spread in the human host."],["dc.identifier.doi","10.1371/journal.pone.0035876"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7927"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60485"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Influenza and SARS-Coronavirus Activating Proteases TMPRSS2 and HAT Are Expressed at Multiple Sites in Human Respiratory and Gastrointestinal Tracts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0176597"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Zmora, Pawel"],["dc.contributor.author","Molau-Blazejewska, Paulina"],["dc.contributor.author","Bertram, Stephanie"],["dc.contributor.author","Walendy-Gnirß, Kerstin"],["dc.contributor.author","Nehlmeier, Inga"],["dc.contributor.author","Hartleib, Anika"],["dc.contributor.author","Moldenhauer, Anna-Sophie"],["dc.contributor.author","Konzok, Sebastian"],["dc.contributor.author","Dehmel, Susann"],["dc.contributor.author","Sewald, Katherina"],["dc.contributor.author","Brinkmann, Constantin"],["dc.contributor.author","Curths, Christoph"],["dc.contributor.author","Knauf, Sascha"],["dc.contributor.author","Gruber, Jens"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Dahlmann, Franziska"],["dc.contributor.author","Braun, Armin"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2019-07-09T11:43:23Z"],["dc.date.available","2019-07-09T11:43:23Z"],["dc.date.issued","2017"],["dc.description.abstract","The cellular serine protease TMPRSS2, a member of the type II transmembrane serine protease (TTSP) family, cleaves and activates the hemagglutinin of influenza A viruses (FLUAV) in cell culture and is essential for spread of diverse FLUAV in mice. Non-human primates (NHP), in particular rhesus and cynomolgus macaques, serve as animal models for influenza and experimental FLUAV infection of common marmosets has recently also been reported. However, it is currently unknown whether the NHP orthologues of human TMPRSS2 cleave and activate FLUAV hemagglutinin and contribute to viral spread in respiratory tissue. Here, we cloned and functionally analyzed the macaque and marmoset orthologues of human TMPRSS2. In addition, we analyzed the macaque orthologues of human TMPRSS4 and HAT, which also belong to the TTSP family. We found that all NHP orthologues of human TMPRSS2, TMPRSS4 and HAT cleave and activate HA upon directed expression and provide evidence that endogenous TMPRSS2 is expressed in the respiratory epithelium of rhesus macaques. Finally, we demonstrate that a serine protease inhibitor active against TMPRSS2 suppresses FLUAV spread in precision-cut lung slices of human, macaque and marmoset origin. These results indicate that FLUAV depends on serine protease activity for spread in diverse NHP and in humans. Moreover, our findings suggest that macaques and marmosets may serve as models to study FLUAV activation by TMPRSS2 in human patients."],["dc.identifier.doi","10.1371/journal.pone.0176597"],["dc.identifier.pmid","28493964"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14490"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58876"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Non-human primate orthologues of TMPRSS2 cleave and activate the influenza virus hemagglutinin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]