Pöhlmann, Stefan

[["dc.bibliographiccitation.artnumber","S2211124721008287"],["dc.bibliographiccitation.firstpage","109415"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Hofmann-Winkler, Heike"],["dc.contributor.author","Krüger, Nadine"],["dc.contributor.author","Kempf, Amy"],["dc.contributor.author","Nehlmeier, Inga"],["dc.contributor.author","Graichen, Luise"],["dc.contributor.author","Arora, Prerna"],["dc.contributor.author","Sidarovich, Anzhalika"],["dc.contributor.author","Moldenhauer, Anna-Sophie"],["dc.contributor.author","Winkler, Martin S."],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2021-09-01T06:42:36Z"],["dc.date.available","2021-09-01T06:42:36Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.celrep.2021.109415"],["dc.identifier.pii","S2211124721008287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89097"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation.issn","2211-1247"],["dc.title","SARS-CoV-2 variant B.1.617 is resistant to bamlanivimab and evades antibodies induced by infection and vaccination"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10636"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Krüger, Nadine"],["dc.contributor.author","Rocha, Cheila"],["dc.contributor.author","Runft, Sandra"],["dc.contributor.author","Krüger, Johannes"],["dc.contributor.author","Färber, Iris"],["dc.contributor.author","Armando, Federico"],["dc.contributor.author","Leitzen, Eva"],["dc.contributor.author","Brogden, Graham"],["dc.contributor.author","Gerold, Gisa"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Baumgärtner, Wolfgang"],["dc.date.accessioned","2021-12-01T09:24:06Z"],["dc.date.available","2021-12-01T09:24:06Z"],["dc.date.issued","2021"],["dc.description.abstract","Natural or experimental infection of domestic cats and virus transmission from humans to captive predatory cats suggest that felids are highly susceptible to SARS-CoV-2 infection. However, it is unclear which cells and compartments of the respiratory tract are infected. To address this question, primary cell cultures derived from the nose, trachea, and lungs of cat and lion were inoculated with SARS-CoV-2. Strong viral replication was observed for nasal mucosa explants and tracheal air–liquid interface cultures, whereas replication in lung slices was less efficient. Infection was mainly restricted to epithelial cells and did not cause major pathological changes. Detection of high ACE2 levels in the nose and trachea but not lung further suggests that susceptibility of feline tissues to SARS-CoV-2 correlates with ACE2 expression. Collectively, this study demonstrates that SARS-CoV-2 can efficiently replicate in the feline upper respiratory tract ex vivo and thus highlights the risk of SARS-CoV-2 spillover from humans to felids."],["dc.description.abstract","Natural or experimental infection of domestic cats and virus transmission from humans to captive predatory cats suggest that felids are highly susceptible to SARS-CoV-2 infection. However, it is unclear which cells and compartments of the respiratory tract are infected. To address this question, primary cell cultures derived from the nose, trachea, and lungs of cat and lion were inoculated with SARS-CoV-2. Strong viral replication was observed for nasal mucosa explants and tracheal air–liquid interface cultures, whereas replication in lung slices was less efficient. Infection was mainly restricted to epithelial cells and did not cause major pathological changes. Detection of high ACE2 levels in the nose and trachea but not lung further suggests that susceptibility of feline tissues to SARS-CoV-2 correlates with ACE2 expression. Collectively, this study demonstrates that SARS-CoV-2 can efficiently replicate in the feline upper respiratory tract ex vivo and thus highlights the risk of SARS-CoV-2 spillover from humans to felids."],["dc.identifier.doi","10.3390/ijms221910636"],["dc.identifier.pii","ijms221910636"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94846"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","1422-0067"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","The Upper Respiratory Tract of Felids Is Highly Susceptible to SARS-CoV-2 Infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Virology"],["dc.bibliographiccitation.volume","93"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Nehlmeier, Inga"],["dc.contributor.author","Brinkmann, Constantin"],["dc.contributor.author","Krähling, Verena"],["dc.contributor.author","Behner, Laura"],["dc.contributor.author","Moldenhauer, Anna-Sophie"],["dc.contributor.author","Krüger, Nadine"],["dc.contributor.author","Nehls, Julia"],["dc.contributor.author","Schindler, Michael"],["dc.contributor.author","Hoenen, Thomas"],["dc.contributor.author","Maisner, Andrea"],["dc.contributor.author","Becker, Stephan"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.editor","Dermody, Terence S."],["dc.date.accessioned","2020-12-10T18:37:03Z"],["dc.date.available","2020-12-10T18:37:03Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1128/JVI.01821-18"],["dc.identifier.eissn","1098-5514"],["dc.identifier.issn","0022-538X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76825"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Tetherin Inhibits Nipah Virus but Not Ebola Virus Replication in Fruit Bat Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.artnumber","103255"],["dc.bibliographiccitation.journal","EBioMedicine"],["dc.bibliographiccitation.volume","65"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Hofmann-Winkler, Heike"],["dc.contributor.author","Smith, Joan C."],["dc.contributor.author","Krüger, Nadine"],["dc.contributor.author","Arora, Prerna"],["dc.contributor.author","Sørensen, Lambert K."],["dc.contributor.author","Søgaard, Ole S."],["dc.contributor.author","Hasselstrøm, Jørgen Bo"],["dc.contributor.author","Winkler, Michael"],["dc.contributor.author","Hempel, Tim"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2022-10-06T13:33:05Z"],["dc.date.available","2022-10-06T13:33:05Z"],["dc.date.issued","2021"],["dc.description.sponsorship"," http://dx.doi.org/10.13039/100010663 ERC"],["dc.description.sponsorship"," http://dx.doi.org/10.13039/501100004937 Bundesministerium fur Bildung und Forschung Dienststelle Berlin"],["dc.description.sponsorship"," http://dx.doi.org/10.13039/501100001659 Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.1016/j.ebiom.2021.103255"],["dc.identifier.pii","S2352396421000487"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115541"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","2352-3964"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Camostat mesylate inhibits SARS-CoV-2 activation by TMPRSS2-related proteases and its metabolite GBPA exerts antiviral activity"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","155"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Emerging Microbes & Infections"],["dc.bibliographiccitation.lastpage","168"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Kleine-Weber, Hannah"],["dc.contributor.author","Schroeder, Simon"],["dc.contributor.author","Krüger, Nadine"],["dc.contributor.author","Prokscha, Alexander"],["dc.contributor.author","Naim, Hassan Y."],["dc.contributor.author","Müller, Marcel A."],["dc.contributor.author","Drosten, Christian"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Hoffmann, Markus"],["dc.date.accessioned","2020-12-10T18:15:34Z"],["dc.date.available","2020-12-10T18:15:34Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1080/22221751.2020.1713705"],["dc.identifier.eissn","2222-1751"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74887"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","030098582110736"],["dc.bibliographiccitation.journal","Veterinary Pathology / Veterinary / American College of Veterinary Pathologists"],["dc.contributor.author","Runft, Sandra"],["dc.contributor.author","Färber, Iris"],["dc.contributor.author","Krüger, Johannes"],["dc.contributor.author","Krüger, Nadine"],["dc.contributor.author","Armando, Federico"],["dc.contributor.author","Rocha, Cheila"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Burigk, Laura"],["dc.contributor.author","Leitzen, Eva"],["dc.contributor.author","Ciurkiewicz, Malgorzata"],["dc.contributor.author","Baumgärtner, Wolfgang"],["dc.date.accessioned","2022-04-01T10:03:04Z"],["dc.date.available","2022-04-01T10:03:04Z"],["dc.date.issued","2022"],["dc.description.abstract","The emergence of the coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inspired rapid research efforts targeting the host range, pathogenesis and transmission mechanisms, and the development of antiviral strategies. Genetically modified mice, rhesus macaques, ferrets, and Syrian golden hamsters have been frequently used in studies of pathogenesis and efficacy of antiviral compounds and vaccines. However, alternatives to in vivo experiments, such as immortalized cell lines, primary respiratory epithelial cells cultured at an air–liquid interface, stem/progenitor cell-derived organoids, or tissue explants, have also been used for isolation of SARS-CoV-2, investigation of cytopathic effects, and pathogen–host interactions. Moreover, initial proof-of-concept studies for testing therapeutic agents can be performed with these tools, showing that animal-sparing cell culture methods could significantly reduce the need for animal models in the future, following the 3R principles of replace, reduce, and refine. So far, only few studies using animal-derived primary cells or tissues have been conducted in SARS-CoV-2 research, although natural infection has been shown to occur in several animal species. Therefore, the need for in-depth investigations on possible interspecies transmission routes and differences in susceptibility to SARS-CoV-2 is urgent. This review gives an overview of studies employing alternative culture systems like primary cell cultures, tissue explants, or organoids for investigations of the pathophysiology and reverse zoonotic potential of SARS-CoV-2 in animals. In addition, future possibilities of SARS-CoV-2 research in animals, including previously neglected methods like the use of precision-cut lung slices, will be outlined."],["dc.identifier.doi","10.1177/03009858211073678"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106077"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1544-2217"],["dc.relation.issn","0300-9858"],["dc.rights.uri","http://journals.sagepub.com/page/policies/text-and-data-mining-license"],["dc.title","Alternatives to animal models and their application in the discovery of species susceptibility to SARS-CoV-2 and other respiratory infectious pathogens: A review"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]