Schwappach-Pignataro, Blanche

[["dc.bibliographiccitation.firstpage","jcs230094"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Journal of Cell Science"],["dc.bibliographiccitation.volume","132"],["dc.contributor.author","Coy-Vergara, Javier"],["dc.contributor.author","Rivera-Monroy, Jhon"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Lenz, Christof"],["dc.contributor.author","Schwappach, Blanche"],["dc.date.accessioned","2020-12-10T18:41:53Z"],["dc.date.available","2020-12-10T18:41:53Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1242/jcs.230094"],["dc.identifier.pmid","31182645"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16428"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77717"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/291"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/72"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A07: Rolle der TRC40-Maschinerie im Proteostase-Netzwerk von Kardiomyozyten"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | Z02: Massenspektrometrie-basierte Proteomanalyse"],["dc.relation.workinggroup","RG Lenz"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.relation.workinggroup","RG Urlaub (Bioanalytische Massenspektrometrie)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","A trap mutant reveals the physiological client spectrum of TRC40"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","289"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","The Protein Journal"],["dc.bibliographiccitation.lastpage","305"],["dc.bibliographiccitation.volume","38"],["dc.contributor.author","Borgese, Nica"],["dc.contributor.author","Coy-Vergara, Javier"],["dc.contributor.author","Colombo, Sara Francesca"],["dc.contributor.author","Schwappach, Blanche"],["dc.date.accessioned","2020-12-10T14:11:41Z"],["dc.date.available","2020-12-10T14:11:41Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1007/s10930-019-09845-4"],["dc.identifier.pmid","31203484"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71163"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/274"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/73"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A07: Rolle der TRC40-Maschinerie im Proteostase-Netzwerk von Kardiomyozyten"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.title","The Ways of Tails: the GET Pathway and more"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","overview_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","39464"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Rivera-Monroy, Jhon"],["dc.contributor.author","Musiol, Lena"],["dc.contributor.author","Unthan-Fechner, Kirsten"],["dc.contributor.author","Farkas, Ákos"],["dc.contributor.author","Clancy, Anne"],["dc.contributor.author","Coy-Vergara, Javier"],["dc.contributor.author","Weill, Uri"],["dc.contributor.author","Gockel, Sarah"],["dc.contributor.author","Lin, Shuh-Yow"],["dc.contributor.author","Corey, David P."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Ströbel, Philipp"],["dc.contributor.author","Schuldiner, Maya"],["dc.contributor.author","Schwappach, Blanche"],["dc.contributor.author","Vilardi, Fabio"],["dc.date.accessioned","2018-04-23T11:49:05Z"],["dc.date.available","2018-04-23T11:49:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Tail-anchored (TA) proteins are post-translationally inserted into membranes. The TRC40 pathway targets TA proteins to the endoplasmic reticulum via a receptor comprised of WRB and CAML. TRC40 pathway clients have been identified using in vitro assays, however, the relevance of the TRC40 pathway in vivo remains unknown. We followed the fate of TA proteins in two tissue-specific WRB knockout mouse models and found that their dependence on the TRC40 pathway in vitro did not predict their reaction to receptor depletion in vivo. The SNARE syntaxin 5 (Stx5) was extremely sensitive to disruption of the TRC40 pathway. Screening yeast TA proteins with mammalian homologues, we show that the particular sensitivity of Stx5 is conserved, possibly due to aggregation propensity of its cytoplasmic domain. We establish that Stx5 is an autophagy target that is inefficiently membrane-targeted by alternative pathways. Our results highlight an intimate relationship between the TRC40 pathway and cellular proteostasis."],["dc.identifier.doi","10.1038/srep39464"],["dc.identifier.gro","3142486"],["dc.identifier.pmid","28000760"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14116"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13638"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/187"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/8"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A06: Molekulare Grundlagen mitochondrialer Kardiomyopathien"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.issn","2045-2322"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","72"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Cell"],["dc.bibliographiccitation.lastpage","86.e7"],["dc.bibliographiccitation.volume","80"],["dc.contributor.author","McDowell, Melanie A."],["dc.contributor.author","Heimes, Michael"],["dc.contributor.author","Fiorentino, Francesco"],["dc.contributor.author","Mehmood, Shahid"],["dc.contributor.author","Farkas, Ákos"],["dc.contributor.author","Coy-Vergara, Javier"],["dc.contributor.author","Wu, Di"],["dc.contributor.author","Bolla, Jani Reddy"],["dc.contributor.author","Schmid, Volker"],["dc.contributor.author","Heinze, Roger"],["dc.contributor.author","Wild, Klemens"],["dc.contributor.author","Flemming, Dirk"],["dc.contributor.author","Pfeffer, Stefan"],["dc.contributor.author","Schwappach, Blanche"],["dc.contributor.author","Robinson, Carol V."],["dc.contributor.author","Sinning, Irmgard"],["dc.date.accessioned","2021-04-14T08:23:23Z"],["dc.date.available","2021-04-14T08:23:23Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1016/j.molcel.2020.08.012"],["dc.identifier.pmid","32910895"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80894"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/69"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/127"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies"],["dc.relation.issn","1097-2765"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.title","Structural Basis of Tail-Anchored Membrane Protein Biogenesis by the GET Insertase Complex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","139"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The EMBO journal"],["dc.bibliographiccitation.lastpage","159"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Richter, Katharina N."],["dc.contributor.author","Revelo, Natalia H."],["dc.contributor.author","Seitz, Katharina J."],["dc.contributor.author","Helm, Martin S."],["dc.contributor.author","Sarkar, Deblina"],["dc.contributor.author","Saleeb, Rebecca S."],["dc.contributor.author","d'Este, Elisa"],["dc.contributor.author","Eberle, Jessica"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Vogl, Christian"],["dc.contributor.author","Lazaro, Diana F."],["dc.contributor.author","Richter, Frank"],["dc.contributor.author","Coy-Vergara, Javier"],["dc.contributor.author","Coceano, Giovanna"],["dc.contributor.author","Boyden, Edward S."],["dc.contributor.author","Duncan, Rory R."],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Outeiro, Tiago F."],["dc.contributor.author","Rehling, Peter"],["dc.contributor.author","Schwappach, Blanche"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Zapiec, Bolek"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.date.accessioned","2018-01-09T14:09:53Z"],["dc.date.available","2018-01-09T14:09:53Z"],["dc.date.issued","2018"],["dc.description.abstract","Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA Despite being less toxic than PFA, and, as most aldehydes, likely usable as a fixative, glyoxal has not yet been systematically tried in modern fluorescence microscopy. Here, we tested and optimized glyoxal fixation and surprisingly found it to be more efficient than PFA-based protocols. Glyoxal acted faster than PFA, cross-linked proteins more effectively, and improved the preservation of cellular morphology. We validated glyoxal fixation in multiple laboratories against different PFA-based protocols and confirmed that it enabled better immunostainings for a majority of the targets. Our data therefore support that glyoxal can be a valuable alternative to PFA for immunostaining."],["dc.identifier.doi","10.15252/embj.201695709"],["dc.identifier.pmid","29146773"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15063"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11599"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/195"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/15"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation"],["dc.relation.eissn","1460-2075"],["dc.relation.workinggroup","RG Hell"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Rehling (Mitochondrial Protein Biogenesis)"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]