Zautner, Andreas Erich

[["dc.bibliographiccitation.journal","Mycoses"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Folba, Claudia"],["dc.contributor.author","Ichsan, Ichsan"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:20:59Z"],["dc.date.available","2018-11-07T09:20:59Z"],["dc.date.issued","2013"],["dc.format.extent","5"],["dc.identifier.isi","000322918300017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29007"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","0933-7407"],["dc.title","Mass Spectrometry as a new Tool for Subtyping in Fungi"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2087"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Emele, Matthias F."],["dc.contributor.author","Joppe, Felix M."],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Kusumawati, R. Lia"],["dc.contributor.author","Berger, Fabian K."],["dc.contributor.author","Laukien, Friederike"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2019-09-24T08:07:22Z"],["dc.date.available","2019-09-24T08:07:22Z"],["dc.date.issued","2019"],["dc.description.abstract","Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system. During the past decade, hypervirulent PCR-ribotypes (RT) e.g., RT027 or RT176 emerged rapidly all over the world, associated with both, increased severity and mortality rates. It is thus of great importance to identify epidemic strains such as RT027 and RT176 as fast as possible. While commonly used diagnostic methods, e.g., multilocus sequence typing (MLST) or PCR-ribotyping, are time-consuming, proteotyping offers a fast, inexpensive, and reliable alternative solution. In this study, we established a MALDI-TOF-based typing scheme for C. difficile. A total of 109 ribotyped strains representative for five MLST clades were analyzed by MALDI-TOF. MLST, based on whole genome sequences, and PCR-ribotyping were used as reference methods. Isoforms of MS-detectable biomarkers, typically ribosomal proteins, were related with the deduced amino acid sequences and added to the C. difficile proteotyping scheme. In total, we were able to associate nine biomarkers with their encoding genes and include them in our proteotyping scheme. The discriminatory capacity of the C. difficile proteotyping scheme was mainly based on isoforms of L28-M (2 main isoforms), L35-M (4 main isoforms), and S20-M (2 main isoforms) giving rise to at least 16 proteotyping-derived types. In our test population, five of these 16 proteotyping-derived types were detected. These five proteotyping-derived types did not correspond exactly to the included five MLST-based C. difficile clades, nevertheless the subtyping depth of both methods was equivalent. Most importantly, proteotyping-derived clade B contained only isolates of the hypervirulent RT027 and RT176. Proteotyping is a stable and easy-to-perform intraspecies typing method and a promising alternative to currently used molecular techniques. It is possible to distinguish the group of RT027 and RT176 isolates from non-RT027/non-RT176 isolates using proteotyping, providing a valuable diagnostic tool."],["dc.identifier.doi","10.3389/fmicb.2019.02087"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16398"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62451"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Proteotyping of Clostridioides difficile as Alternate Typing Method to Ribotyping Is Able to Distinguish the Ribotypes RT027 and RT176 From Other Ribotypes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","302"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Welcker, F."],["dc.contributor.author","Edel, B."],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Kuhns, Martin"],["dc.contributor.author","Gross, U."],["dc.contributor.author","Kappe, R."],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Rimek, D."],["dc.date.accessioned","2018-11-07T09:06:05Z"],["dc.date.available","2018-11-07T09:06:05Z"],["dc.date.issued","2012"],["dc.format.extent","117"],["dc.identifier.isi","000311593300422"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25475"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","64th Annual Meeting of the German-Society-for-Hygiene-and-Microbiology (DGHM)"],["dc.relation.eventlocation","Hamburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Use of MALDI-TOF mass spectrometry for typing Aspergillus flavus in a potential hospital outbreak scenario"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1088"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Goldschmidt, Anne-Marie"],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Schuldes, Jörg"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Stingl, Kerstin"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Lingner, Thomas"],["dc.date.accessioned","2018-11-07T09:47:24Z"],["dc.date.available","2018-11-07T09:47:24Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: Campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. In contrast to Campylobacter jejuni, about 5 % of Campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by DpnI cutting specifically 5'-G(m)ATC-3' motifs. This indicates significant differences in DNA methylation between both microbial species. The goal of the study was to analyze the methylome of a C. coli strain susceptible to DpnI digestion, to identify its methylation motifs and restriction modification systems (RM-systems), and compare them to related organisms like C. jejuni and Helicobacter pylori. Results: Using one SMRT cell and the PacBio RS sequencing technology followed by PacBio Modification and Motif Analysis the complete genome of the DpnI susceptible strain C. coli BfR-CA-9557 was sequenced to 500-fold coverage and assembled into a single contig of 1.7 Mbp. The genome contains a CJIE1-like element prophage and is phylogenetically closer to C. coli clade 1 isolates than clade 3. 45,881 6-methylated adenines (ca. 2.7 % of genome positions) that are predominantly arranged in eight different methylation motifs and 1,788 4-methylated cytosines (ca. 0.1 %) have been detected. Only two of these motifs correspond to known restriction modification motifs. Characteristic for this methylome was the very high fraction of methylation of motifs with mostly above 99 %. Conclusions: Only five dominant methylation motifs have been identified in C. jejuni, which have been associated with known RM-systems. C. coli BFR-CA-9557 shares one (RAATTY) of these, but four ORFs could be assigned to putative Type I RM-systems, seven ORFs to Type II RM-systems and three ORFs to Type IV RM-systems. In accordance with DpnI prescreening RM-system IIP, methylation of GATC motifs was detected in C. coli BfR-CA-9557. A homologous IIP RM-system has been described for H. pylori. The remaining methylation motifs are specific for C. coli BfR-CA-9557 and have been neither detected in C. jejuni nor in H. pylori. The results of this study give us new insights into epigenetics of Campylobacteraceae and provide the groundwork to resolve the function of RM-systems in C. coli."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1186/s12864-015-2317-3"],["dc.identifier.isi","000367061700011"],["dc.identifier.pmid","26689587"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13469"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35106"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4163"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Journal of Clinical Microbiology"],["dc.bibliographiccitation.lastpage","4167"],["dc.bibliographiccitation.volume","52"],["dc.contributor.author","Bernhard, Mareike"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:32:15Z"],["dc.date.available","2018-11-07T09:32:15Z"],["dc.date.issued","2014"],["dc.description.abstract","Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based species identification has become a reliable and fast tool for use in clinical diagnostics, including in mycology. To identify yeasts in the MALDI Biotyper system, a multistep extraction protocol, which is also used to generate the reference spectra, is recommended. Sample preparation by on-target lysis (OTL) requires significantly less hands-on time and is therefore highly desirable, but it results in too-low MALDI Biotyper log score values to allow automated species identification. To overcome this problem, we developed a procedure for generating and validating an OTL spectrum data set for the most relevant and frequently occurring yeast species in clinical specimens. The performance was evaluated against a set of OTL spectra derived during clinical routine procedures and from a set of closely related yeasts. In the diagnostic setting, the OTL procedure significantly decreased the workload but allowed species identification with high specificity and sensitivity. False identifications were not observed. The use of in-house-generated OTL reference spectra can highly accelerate MALDI-TOF MS-based yeast species identification using the MALDI Biotyper."],["dc.description.sponsorship","Bruker Daltonics"],["dc.identifier.doi","10.1128/JCM.02128-14"],["dc.identifier.isi","345222900007"],["dc.identifier.pmid","25232169"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31711"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1098-660X"],["dc.relation.issn","0095-1137"],["dc.title","Yeast On-Target Lysis (YOTL), a Procedure for Making Auxiliary Mass Spectrum Data Sets for Clinical Routine Identification of Yeasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","13431"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:53:08Z"],["dc.date.available","2018-11-07T09:53:08Z"],["dc.date.issued","2015"],["dc.description.abstract","MALDI-TOF-MS of microorganisms, which identifies microbes based on masses of high abundant low molecular weight proteins, is rapidly advancing to become another standard method in clinical routine laboratory diagnostics. Allelic isoforms of these proteins result in varying masses of detectable biomarker ions. These variations give rise to a novel typing method for microorganisms named mass spectrometry-based phyloproteomics (MSPP). The base of MSPP is an amino acid sequence list of allelic isoforms caused by non-synonymous mutations in biomarker genes, which were detectable as mass shifts in an overlay of calibrated MALDI-TOF spectra. Thus, for each isolate a combination of amino acid sequences can be deduced from the scheme of recordable biomarker masses. Performing comparably to laborious multilocus and whole genome sequence typing (wgMLST)-approaches it is feasible to build phyloproteomic dendrograms using hierarchical cluster analysis. MSPP bears a high potential especially for identification of chromosomal localised virulence or antimicrobial resistance factors associated with evolutionary relatedness. In this study the principle of MSPP-typing was demonstrated on a Campylobacter jejuni ssp. jejuni isolate collection and MSPP was compared to MLST."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft; Georg August Universitat Gottingen"],["dc.identifier.doi","10.1038/srep13431"],["dc.identifier.isi","000360037600002"],["dc.identifier.pmid","26303099"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12455"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36271"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Mass Spectrometry-based PhyloProteomics (MSPP): A novel microbial typing Method"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","302"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Kuhns, Martin"],["dc.contributor.author","Rabsch, Wolfgang"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:06:03Z"],["dc.date.available","2018-11-07T09:06:03Z"],["dc.date.issued","2012"],["dc.format.extent","25"],["dc.identifier.isi","000311593300084"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25465"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.eventlocation","Hamburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Rapid discrimination of Salmonella enterica serovar Typhi from other serovars by MALDI-TOF mass spectrometry"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","162"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Fungal Biology"],["dc.bibliographiccitation.lastpage","165"],["dc.bibliographiccitation.volume","120"],["dc.contributor.author","Bernhard, Mareike"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Steinmann, Joerg"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T10:18:47Z"],["dc.date.available","2018-11-07T10:18:47Z"],["dc.date.issued","2016"],["dc.description.abstract","MALDI-ToF mass spectrometry offers fast and reliable species identification for bacteria and yeasts under clinical routine conditions. Here, we produced mass spectra for identification of clinically important species of the Pseudallescheria/Scedosporium complex using the recently suggested new nomenclature and use this example to discuss to what extent the principle of DNA barcoding might be transferred to mass spectrometry. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.funbio.2015.07.001"],["dc.identifier.isi","000369551600005"],["dc.identifier.pmid","26781372"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41522"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","1878-6162"],["dc.relation.issn","1878-6146"],["dc.title","Towards proteomic species barcoding of fungi-An example using Scedosporium/Pseudallescheria complex isolates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","European Journal of Microbiology and Immunology"],["dc.bibliographiccitation.lastpage","10"],["dc.contributor.author","Emele, Matthias F."],["dc.contributor.author","Karg, Matti"],["dc.contributor.author","Hotzel, Helmut"],["dc.contributor.author","Graaf-van Bloois, Linda"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2019-07-09T11:51:43Z"],["dc.date.available","2019-07-09T11:51:43Z"],["dc.date.issued","2019"],["dc.description.abstract","ampylobacter fetus is a causative agent of intestinal illness and, occasionally, severe systemic infections and meningitis. C. fetus currently comprises three subspecies: C. fetus subspecies fetus (Cff), C. fetus subspecies venerealis (Cfv), and C. fetus subspecies testudinum (Cft). Cff and Cfv are primarily associated with mammals whereas Cft is associated with reptiles. To offer an alternative to laborious sequence-based techniques such as multilocus sequence typing (MLST) and polymerase chain reaction (PCR)-ribotyping for this species, the purpose of the study was to develop a typing scheme based on proteotyping. In total, 41 representative C. fetus strains were analyzed by intact cell mass spectrometry and compared to MLST results. Biomarkers detected in the mass spectrum of C. fetus subsp. fetus reference strain LMG 6442 (NCTC 10842) as well as corresponding isoforms were associated with the respective amino acid sequences and added to the C. fetus proteotyping scheme. In combination, the 9 identified biomarkers allow the differentiation of Cft subspecies strains from Cff and Cfv subspecies strains. Biomarkers to distinguish between Cff and Cfv were not found. The results of the study show the potential of proteotyping to differentiate different subspecies, but also the limitations of the method."],["dc.identifier.doi","10.1556/1886.2019.00006"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16175"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59994"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2062-8633"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject.ddc","610"],["dc.title","Differentiation of Campylobacter fetus subspecies by proteotyping"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","80"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","81"],["dc.bibliographiccitation.volume","303"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Tareen, Abdul Malik"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gross, U."],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:20:05Z"],["dc.date.available","2018-11-07T09:20:05Z"],["dc.date.issued","2013"],["dc.identifier.isi","000331497600276"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28794"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","65th Annual Meeting of the German-Society-for-Hygiene-and-Microbiology (DGHM) e V / Annual Meeting of the German-Society-for-Infectious-Diseases (DGI) e V"],["dc.relation.eventlocation","Univ Rostock, Rostock, GERMANY"],["dc.relation.issn","1618-0607"],["dc.relation.issn","1438-4221"],["dc.title","Phyloproteomics versus phylogenetics: a comparative approach for the Discrimination of Campylobacter jejuni Subpopulations"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]