Zautner, Andreas Erich

[["dc.bibliographiccitation.artnumber","375681"],["dc.bibliographiccitation.journal","BioMed Research International"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.date.accessioned","2018-11-07T09:45:32Z"],["dc.date.available","2018-11-07T09:45:32Z"],["dc.date.issued","2014"],["dc.description.abstract","Atypical and multidrug resistance, especially ESBL and carbapenemase expressing Enterobacteriaceae, is globally spreading. Therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy. Consequently, rapid antibiotic resistance testing is essential. Various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data. PCR- and sequencing-based approaches are the most expensive but the most frequently applied modes of testing, suitable for the detection of resistance genes even from primary material. Next generation sequencing, based either on assessment of allelic single nucleotide polymorphisms or on the detection of nonubiquitous resistance mechanisms might allow for sequence-based bacterial resistance testing comparable to viral resistance testing on the long term. Fluorescence in situ hybridization (FISH), based on specific binding of fluorescence-labeled oligonucleotide probes, provides a less expensive molecular bridging technique. It is particularly useful for detection of resistance mechanisms based on mutations in ribosomal RNA. Approaches based on MALDI-TOFMS, alone or in combination with molecular techniques, like PCR/electrospray ionization MS or minisequencing provide the fastest resistance results from pure colonies or even primary samples with a growing number of protocols. This review details the various approaches of rapid resistance testing, their pros and cons, and their potential use for the diagnostic laboratory."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft; Georg August Universitat Gottingen"],["dc.identifier.doi","10.1155/2014/375681"],["dc.identifier.isi","000346708400001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10892"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34641"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Hindawi Publishing Corporation"],["dc.relation.issn","2314-6141"],["dc.relation.issn","2314-6133"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1"],["dc.bibliographiccitation.journal","Otolaryngology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.other","Ulualp, Seckin O."],["dc.date.accessioned","2013-01-04T13:06:45Z"],["dc.date.accessioned","2021-10-27T13:19:58Z"],["dc.date.available","2013-01-04T13:06:45Z"],["dc.date.available","2021-10-27T13:19:58Z"],["dc.date.issued","2012"],["dc.description.abstract","The article provides an overview on the current state-of-science of middle ear cholesteatoma, a non-neoplastic, keratinizing lesion that is characterized by the proliferation of epithelium with aberrant micro-architecture. Pathogenetic mechanisms including morphological, immunological, epidemiological and microbiological aspects of the disease are summarized. The importance of penicillinase-expressing anaerobic bacteria and biofilm formation for maintaining the chronic middle ear inflammation is stressed. Nevertheless, the role of the isolated pathogens in the primarily non-sterile compartiment of the middle ear cavity is so far not completely understood and data on the isolated species are contradictious. Heredity was demonstated for some variants of the disease. Therefore, further studies on the etiological role of microbial agents and potential benefits of resistance-adapted antimicrobial therapy seem advisable. Local and systemic complications of the potentially life-threatening disease like conductive and sensorineural hearing loss and cranial abscesses are reported. The prognosis is limited due to frequent recurrence in spite of surgical therapy. Further research is necessary for a better understanding of the pathogenetic mechanisms and to expand the sectrum of therapeutic options."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.4172/2161-119X.S5-001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8474"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91926"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","2161-119X"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Cholesteatoma – A Potential Consequence of Chronic Middle Ear Inflammation"],["dc.title.alternative","Otilis Media"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","40"],["dc.bibliographiccitation.journal","Acta Tropica"],["dc.bibliographiccitation.lastpage","65"],["dc.bibliographiccitation.volume","165"],["dc.contributor.author","Schwarz, Norbert Georg"],["dc.contributor.author","Loderstaedt, Ulrike"],["dc.contributor.author","Hahn, Andreas"],["dc.contributor.author","Hinz, Rebecca"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Eibach, Daniel"],["dc.contributor.author","Fischer, Marcellus"],["dc.contributor.author","Hagen, Ralf Matthias"],["dc.contributor.author","Frickmann, Hagen"],["dc.date.accessioned","2018-11-07T10:29:30Z"],["dc.date.available","2018-11-07T10:29:30Z"],["dc.date.issued","2017"],["dc.description.abstract","This review reports on laboratory diagnostic approaches for selected, highly pathogenic neglected zoonotic diseases, i.e. anthrax, bovine tuberculosis, brucellosis, echinococcosis, leishmaniasis, rabies, Taenia solium-associated diseases (neuro-/cysticercosis & taeniasis) and trypanosomiasis. Diagnostic options, including microscopy, culture, matrix-assisted laser-desorption-ionisation time of-flight mass spectrometry, molecular approaches and serology are introduced. These procedures are critically discussed regarding their diagnostic reliability and state of evaluation. For rare diseases reliable evaluation data are scarce due to the rarity of samples. If bio-safety level 3 is required for cultural growth, but such high standards of laboratory infrastructure are not available, serological and molecular approaches from inactivated sample material might be alternatives. Multiple subsequent testing using various test platforms in a stepwise approach may improve sensitivity and specificity. Cheap and easy to use tests, usually called \"rapid diagnostic tests\" (RDTs) may impact disease control measures, but should not preclude developing countries from state of the art diagnostics. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license."],["dc.identifier.doi","10.1016/j.actatropica.2015.09.003"],["dc.identifier.isi","000390973400008"],["dc.identifier.pmid","26391646"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14301"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43653"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1873-6254"],["dc.relation.issn","0001-706X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Microbiological laboratory diagnostics of neglected zoonotic diseases (NZDs)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","352"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Foodborne Pathogens and Disease"],["dc.bibliographiccitation.lastpage","358"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Dekker, Denise"],["dc.contributor.author","Eibach, Daniel"],["dc.contributor.author","Boahen, Kennedy G."],["dc.contributor.author","Akenten, Charity Wiafe"],["dc.contributor.author","Pfeifer, Yvonne"],["dc.contributor.author","Zautner, Andreas E."],["dc.contributor.author","Mertens, Eva"],["dc.contributor.author","Krumkamp, Ralf"],["dc.contributor.author","Jaeger, Anna"],["dc.contributor.author","Flieger, Antje"],["dc.contributor.author","Owusu-Dabo, Ellis"],["dc.contributor.author","May, Jürgen"],["dc.date.accessioned","2020-12-10T18:16:02Z"],["dc.date.available","2020-12-10T18:16:02Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1089/fpd.2018.2562"],["dc.identifier.eissn","1556-7125"],["dc.identifier.issn","1535-3141"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16618"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75032"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Fluoroquinolone-Resistant Salmonella enterica , Campylobacter spp., and Arcobacter butzleri from Local and Imported Poultry Meat in Kumasi, Ghana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","279"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Animals"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Ushanov, Leonid"],["dc.contributor.author","Lasareishvili, Besarion"],["dc.contributor.author","Janashia, Irakli"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2020-12-10T18:46:55Z"],["dc.date.available","2020-12-10T18:46:55Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/ani10020279"],["dc.identifier.eissn","2076-2615"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17334"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78585"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Application of Campylobacter jejuni Phages: Challenges and Perspectives"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","761259"],["dc.bibliographiccitation.journal","BioMed Research International"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Hinz, Rebecca"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.date.accessioned","2018-11-07T10:03:36Z"],["dc.date.available","2018-11-07T10:03:36Z"],["dc.date.issued","2015"],["dc.description.abstract","Chronic inflammation, which is caused by recurrent infections, is one of the factors contributing to the pathogenesis of cholesteatoma. If reimplantation of autologous ossicles after a surgical intervention is intended, inactivation of planktonic bacteria and biofilms is desirable. High hydrostatic pressure treatment is a procedure, which has been used to inactivate cholesteatoma cells on ossicles. Here we discuss the potential inactivating effect of high hydrostatic pressure on microbial pathogens including biofilms. Recent experimental data suggest an incomplete inactivation at a pressure level, which is tolerable for the bone substance of ossicles and results at least in a considerable reduction of pathogen load. Further studies are necessary to access how far this quantitative reduction of pathogens is sufficient to prevent ongoing chronic infections, for example, due to forming of biofilms."],["dc.description.sponsorship","Open Access Publikationsfonds 2015"],["dc.identifier.doi","10.1155/2015/761259"],["dc.identifier.isi","000349718100001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11856"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38504"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Hindawi Publishing Corp"],["dc.relation.issn","2314-6141"],["dc.relation.issn","2314-6133"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Infectious Causes of Cholesteatoma and Treatment of Infected Ossicles prior to Reimplantation by Hydrostatic High-Pressure Inactivation"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","720"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Microorganisms"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Schwanbeck, Julian"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Hasdemir, Ufuk"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Pfeifer, Yvonne"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Zautner, Andreas E."],["dc.contributor.author","Frickmann, Hagen"],["dc.date.accessioned","2021-06-01T09:42:39Z"],["dc.date.available","2021-06-01T09:42:39Z"],["dc.date.issued","2021"],["dc.description.abstract","Mobile genetic elements, such as plasmids, facilitate the spread of antibiotic resistance genes in Enterobacterales. In line with this, we investigated the plasmid-resistome of seven blaOXA-48 gene-carrying Klebsiella pneumoniae isolates, which were isolated between 2013 and 2014 at the University Medical Center in Göttingen, Germany. All isolates were subjected to complete genome sequencing including the reconstruction of entire plasmid sequences. In addition, phenotypic resistance testing was conducted. The seven isolates comprised both disease-associated isolates and colonizers isolated from five patients. They fell into two clusters of three sequence type (ST)101 and two ST11 isolates, respectively; and ST15 and ST23 singletons. The seven isolates harbored various plasmids of the incompatibility (Inc) groups IncF, IncL/M, IncN, IncR, and a novel plasmid chimera. All blaOXA-48 genes were encoded on the IncL/M plasmids. Of note, distinct phenotypical resistance patterns associated with different sets of resistance genes encoded by IncL/M and IncR plasmids were observed among isolates of the ST101 cluster in spite of high phylogenetic relatedness of the bacterial chromosomes, suggesting nosocomial transmission. This highlights the importance of plasmid uptake and plasmid recombination events for the fast generation of resistance variability after clonal transmission. In conclusion, this study contributes a piece in the puzzle of molecular epidemiology of resistance gene-carrying plasmids in K. pneumoniae in Germany."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/microorganisms9040720"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85312"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2076-2607"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights","CC BY 4.0"],["dc.title","Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2087"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Emele, Matthias F."],["dc.contributor.author","Joppe, Felix M."],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Kusumawati, R. Lia"],["dc.contributor.author","Berger, Fabian K."],["dc.contributor.author","Laukien, Friederike"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2019-09-24T08:07:22Z"],["dc.date.available","2019-09-24T08:07:22Z"],["dc.date.issued","2019"],["dc.description.abstract","Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system. During the past decade, hypervirulent PCR-ribotypes (RT) e.g., RT027 or RT176 emerged rapidly all over the world, associated with both, increased severity and mortality rates. It is thus of great importance to identify epidemic strains such as RT027 and RT176 as fast as possible. While commonly used diagnostic methods, e.g., multilocus sequence typing (MLST) or PCR-ribotyping, are time-consuming, proteotyping offers a fast, inexpensive, and reliable alternative solution. In this study, we established a MALDI-TOF-based typing scheme for C. difficile. A total of 109 ribotyped strains representative for five MLST clades were analyzed by MALDI-TOF. MLST, based on whole genome sequences, and PCR-ribotyping were used as reference methods. Isoforms of MS-detectable biomarkers, typically ribosomal proteins, were related with the deduced amino acid sequences and added to the C. difficile proteotyping scheme. In total, we were able to associate nine biomarkers with their encoding genes and include them in our proteotyping scheme. The discriminatory capacity of the C. difficile proteotyping scheme was mainly based on isoforms of L28-M (2 main isoforms), L35-M (4 main isoforms), and S20-M (2 main isoforms) giving rise to at least 16 proteotyping-derived types. In our test population, five of these 16 proteotyping-derived types were detected. These five proteotyping-derived types did not correspond exactly to the included five MLST-based C. difficile clades, nevertheless the subtyping depth of both methods was equivalent. Most importantly, proteotyping-derived clade B contained only isolates of the hypervirulent RT027 and RT176. Proteotyping is a stable and easy-to-perform intraspecies typing method and a promising alternative to currently used molecular techniques. It is possible to distinguish the group of RT027 and RT176 isolates from non-RT027/non-RT176 isolates using proteotyping, providing a valuable diagnostic tool."],["dc.identifier.doi","10.3389/fmicb.2019.02087"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16398"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62451"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Proteotyping of Clostridioides difficile as Alternate Typing Method to Ribotyping Is Able to Distinguish the Ribotypes RT027 and RT176 From Other Ribotypes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1088"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Goldschmidt, Anne-Marie"],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Schuldes, Jörg"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Stingl, Kerstin"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Lingner, Thomas"],["dc.date.accessioned","2018-11-07T09:47:24Z"],["dc.date.available","2018-11-07T09:47:24Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: Campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. In contrast to Campylobacter jejuni, about 5 % of Campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by DpnI cutting specifically 5'-G(m)ATC-3' motifs. This indicates significant differences in DNA methylation between both microbial species. The goal of the study was to analyze the methylome of a C. coli strain susceptible to DpnI digestion, to identify its methylation motifs and restriction modification systems (RM-systems), and compare them to related organisms like C. jejuni and Helicobacter pylori. Results: Using one SMRT cell and the PacBio RS sequencing technology followed by PacBio Modification and Motif Analysis the complete genome of the DpnI susceptible strain C. coli BfR-CA-9557 was sequenced to 500-fold coverage and assembled into a single contig of 1.7 Mbp. The genome contains a CJIE1-like element prophage and is phylogenetically closer to C. coli clade 1 isolates than clade 3. 45,881 6-methylated adenines (ca. 2.7 % of genome positions) that are predominantly arranged in eight different methylation motifs and 1,788 4-methylated cytosines (ca. 0.1 %) have been detected. Only two of these motifs correspond to known restriction modification motifs. Characteristic for this methylome was the very high fraction of methylation of motifs with mostly above 99 %. Conclusions: Only five dominant methylation motifs have been identified in C. jejuni, which have been associated with known RM-systems. C. coli BFR-CA-9557 shares one (RAATTY) of these, but four ORFs could be assigned to putative Type I RM-systems, seven ORFs to Type II RM-systems and three ORFs to Type IV RM-systems. In accordance with DpnI prescreening RM-system IIP, methylation of GATC motifs was detected in C. coli BfR-CA-9557. A homologous IIP RM-system has been described for H. pylori. The remaining methylation motifs are specific for C. coli BfR-CA-9557 and have been neither detected in C. jejuni nor in H. pylori. The results of this study give us new insights into epigenetics of Campylobacteraceae and provide the groundwork to resolve the function of RM-systems in C. coli."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1186/s12864-015-2317-3"],["dc.identifier.isi","000367061700011"],["dc.identifier.pmid","26689587"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13469"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35106"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","481"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Antibiotics"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Tilkorn, Friederike K. M. T."],["dc.contributor.author","Frickmann, Hagen"],["dc.contributor.author","Simon, Isabel S."],["dc.contributor.author","Schwanbeck, Julian"],["dc.contributor.author","Horn, Sebastian"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.date.accessioned","2020-08-06T06:04:43Z"],["dc.date.available","2020-08-06T06:04:43Z"],["dc.date.issued","2020"],["dc.description.abstract","Young children are frequently colonized with Clostridioides (C.) difficile. Depending on their resistance patterns, antibiotic treatment can facilitate gastrointestinal spreading in colonized individuals, potentially leading to transmission to others. C. difficile was isolated from stool samples from infants born in two hospitals in Göttingen and Darmstadt, Germany. All isolates were subjected to phenotypic antimicrobial resistance testing, PCR-based screening for toxin genes and mass spectrometry-based exclusion of ribotypes 027 and 176. Within an initial cohort of 324 neonates with a longitudinal survey of C. difficile, 137 strains were isolated from 48 individuals. Antimicrobial resistance was recorded against metronidazole in one (0.7%), erythromycin in 16 (11.7%) and moxifloxacin in 2 (1.5%) of the strains, whereas no resistance was observed against vancomycin (0.0%) or rifampicin (0.0%). Newly observed resistance against erythromycin in children with detection of previously completely sensitive isolates was reported for C. difficile isolates from 2 out of 48 children. In 20 children (42%), non-toxigenic strains were detected, and from 27 children (56%), toxigenic strains were isolated, while both toxigenic and non-toxigenic strains were recorded for 1 child (2%). Ribotypes 027 or 176 were not observed. In conclusion, the German C. difficile strains isolated from the children showed mild to moderate resistance with predominance of macrolide resistance, a substance class which is frequently applied in children. The observed switches to the dominance of macrolide-resistant isolates suggests likely selection of resistant C. difficile strains already in children"],["dc.identifier.doi","10.3390/antibiotics9080481"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17510"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67527"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2079-6382"],["dc.relation.issn","2079-6382"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.gro","C. difficile"],["dc.subject.gro","Rifaximin"],["dc.title","Antimicrobial Resistance Patterns in Clostridioides difficile Strains Isolated from Neonates in Germany"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]