Sperling, Jens

[["dc.bibliographiccitation.journal","Oncology Research and Treatment"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Sperling, Jens"],["dc.contributor.author","Ziemann, C."],["dc.contributor.author","Gittler, Anika"],["dc.contributor.author","Benz-Weisser, Anna"],["dc.contributor.author","Menger, Michael D."],["dc.contributor.author","Kollmar, Otto"],["dc.date.accessioned","2018-11-07T09:44:06Z"],["dc.date.available","2018-11-07T09:44:06Z"],["dc.date.issued","2014"],["dc.format.extent","49"],["dc.identifier.isi","000332306700162"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34323"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.relation.issn","2296-5262"],["dc.relation.issn","2296-5270"],["dc.title","Locoregional application of temsirolimus is effective to inhibit tumor growth of CC531 colorectal liver metastases even after stimulation by hepatectomy or portal branch ligation"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Cancer"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Ziemann, Christian"],["dc.contributor.author","Roller, Jonas"],["dc.contributor.author","Malter, Markus M."],["dc.contributor.author","Keller, Kira"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Glanemann, Matthias"],["dc.contributor.author","Menger, Michael D."],["dc.contributor.author","Sperling, Jens"],["dc.date.accessioned","2020-12-10T18:38:54Z"],["dc.date.available","2020-12-10T18:38:54Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1186/s12885-019-6135-x"],["dc.identifier.eissn","1471-2407"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16951"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77472"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Intra-arterial EmboCept S® and DC Bead® effectively inhibit tumor growth of colorectal rat liver metastases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","555"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","International Journal of Colorectal Disease"],["dc.bibliographiccitation.lastpage","562"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Sperling, Jens"],["dc.contributor.author","Schaefer, Thilo"],["dc.contributor.author","Benz-Weisser, Anna"],["dc.contributor.author","Ziemann, Christian"],["dc.contributor.author","Scheuer, Claudia"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Schilling, Martin Karl"],["dc.contributor.author","Menger, Michael D."],["dc.date.accessioned","2018-11-07T09:26:19Z"],["dc.date.available","2018-11-07T09:26:19Z"],["dc.date.issued","2013"],["dc.description.abstract","Systemic chemotherapy still represents the gold standard in the treatment of irresectable colorectal liver metastases. Modern anticancer agents like the monoclonal antibody cetuximab have improved the outcome of patients in clinical studies. As hepatic arterial infusion (HAI) is capable to potentially increase the anticancer effect of cytostatics, we herein studied whether HAI of cetuximab (CE) as a single agent or in combination with oxaliplatin (OX) exerts increased anticancer effects compared to the systemic application (SYS) of the drugs. WAG/Rij rats were randomized to eight groups and underwent 10 days after subcapsular hepatic tumor implantation either HAI or SYS of CE, OX, or the combination of both agents (CE + OX). Saline-treated animals served as controls. Tumor volume was measured at days 10 and 13 using three-dimensional ultrasound. On day 13, liver and tumor tissue was sampled for histological and immunohistochemical analysis. In controls, the tumor volume significantly increased from day 10 to 13. Application of OX alone via HAI or SYS did not inhibit tumor growth compared to controls. SYS of CE or CE + OX did also not reduce tumor growth. In contrast, HAI of CE and CE + OX significantly inhibited tumor growth. HAI of CE significantly reduced tumor vascularization as measured by the number of platelet endothelial cell adhesion molecule-1-positive cells and significantly increased the number of apoptotic tumor cells as measured by the cellular caspase-3 expression. HAI of CE and CE + OX reduces tumor growth of colorectal rat liver metastases involving the inhibition of angiogenesis and induction of tumor cell apoptosis."],["dc.identifier.doi","10.1007/s00384-012-1617-1"],["dc.identifier.isi","000318368300014"],["dc.identifier.pmid","23242249"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10946"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30274"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","0179-1958"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Hepatic arterial infusion but not systemic application of cetuximab in combination with oxaliplatin significantly reduces growth of CC531 colorectal rat liver metastases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Oncology Research and Treatment"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Ziemann, C."],["dc.contributor.author","Sperling, Jens"],["dc.contributor.author","Malter, M."],["dc.contributor.author","Keller, Katriona"],["dc.contributor.author","Roller, J."],["dc.contributor.author","Dold, S."],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Laschke, M."],["dc.contributor.author","Glanemann, M."],["dc.contributor.author","Menger, Michael D."],["dc.date.accessioned","2018-11-07T09:44:06Z"],["dc.date.available","2018-11-07T09:44:06Z"],["dc.date.issued","2014"],["dc.format.extent","47"],["dc.identifier.isi","000332306700155"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34322"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.relation.issn","2296-5262"],["dc.relation.issn","2296-5270"],["dc.title","Inhibition of tumor growth of colorectal liver metastases after trans-arterial chemoembolization using different chemoembolisats in a rat model"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","323"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Clinical & Experimental Metastasis"],["dc.bibliographiccitation.lastpage","332"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Senger, Sebastian"],["dc.contributor.author","Sperling, Jens"],["dc.contributor.author","Oberkircher, Barbara"],["dc.contributor.author","Schilling, Martin K."],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Menger, Michael D."],["dc.contributor.author","Ziemann, Christian"],["dc.date.accessioned","2020-12-10T14:11:27Z"],["dc.date.available","2020-12-10T14:11:27Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1007/s10585-017-9852-z"],["dc.identifier.eissn","1573-7276"],["dc.identifier.issn","0262-0898"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71078"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Portal branch ligation does not counteract the inhibiting effect of temsirolimus on extrahepatic colorectal metastatic growth"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","587"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Surgical Research"],["dc.bibliographiccitation.lastpage","594"],["dc.bibliographiccitation.volume","185"],["dc.contributor.author","Sperling, Jens"],["dc.contributor.author","Ziemann, Christian"],["dc.contributor.author","Gittler, Anika"],["dc.contributor.author","Benz-Weisser, Anna"],["dc.contributor.author","Menger, Michael D."],["dc.contributor.author","Kollmar, Otto"],["dc.date.accessioned","2018-11-07T09:17:19Z"],["dc.date.available","2018-11-07T09:17:19Z"],["dc.date.issued","2013"],["dc.description.abstract","Background: Hepatic arterial infusion (HAI) of specific anti-tumor drugs can be more effective compared with systemic drug application. Herein, we studied whether HAI of temsirolimus is effective to inhibit tumor growth of colorectal liver metastases after liver resection. Materials and methods: Twenty-four Wistar Albino Glaxo from Rijswijk (WAG/Rij) rats were randomized to four groups and underwent subcapsular implantation of CC531 colorectal cancer cells in the left liver lobe. In two groups, a 70% liver resection (Phx) was performed simultaneously. After 10 d, animals received either a HAI of temsirolimus (CCI-779) or saline solution (controls). Tumor growth was determined on d 10 and 13 using three-dimensional ultrasound. On d 13, tumor tissue was removed for histologic and immunohistochemical analysis. Results: Sham controls revealed a tumor growth of similar to 40% from d 10 to d 13. HAI of temsirolimus completely inhibited this tumor growth. Controls with Phx showed a tumor growth of > 60%. In contrast, HAI of temsirolimus in Phx animals did not only inhibit tumor growth but was even capable of decreasing the tumor size by similar to 8%. Immunohistochemical analysis of the tumors showed a decreased proliferation rate and an increased cleaved caspase-3 activity, which was associated with a significant reduction of platelet endothelial cell adhesion molecule (PECAM)-1-positive cells after HAI of temsirolimus. Conclusions: HAI of temsirolimus inhibits tumor growth of CC531 colorectal liver metastases even if a growth-stimulating procedure like Phx is performed. Inhibition of tumor growth is provided by a decrease of tumor vascularization associated with an inhibition of tumor cell proliferation and an induction of tumor cell apoptosis. (C) 2013 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.jss.2013.06.005"],["dc.identifier.isi","000326938500042"],["dc.identifier.pmid","23845871"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28136"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","1095-8673"],["dc.relation.issn","0022-4804"],["dc.title","Hepatic arterial infusion of temsirolimus inhibits tumor growth of colorectal rat liver metastases even after a growth stimulating procedure like liver resection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","313"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Clinical & Experimental Metastasis"],["dc.bibliographiccitation.lastpage","321"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Sperling, Jens"],["dc.contributor.author","Ziemann, Christian"],["dc.contributor.author","Gittler, Anika"],["dc.contributor.author","Benz-Weisser, Anna"],["dc.contributor.author","Menger, Michael D."],["dc.contributor.author","Kollmar, Otto"],["dc.date.accessioned","2018-11-07T09:59:04Z"],["dc.date.available","2018-11-07T09:59:04Z"],["dc.date.issued","2015"],["dc.description.abstract","Portal branch ligation (PBL) can be performed before major hepatic resection of colorectal liver metastases (mCRC) to increase the remnant liver mass. However, PBL may also stimulate mCRC growth through hepatic arterial hyperperfusion and growth factor release. Herein, we studied whether hepatic arterial infusion (HAI) of the mTOR-inhibitor temsirolimus (Tem) is capable of inhibiting the growth of colorectal liver metastases after PBL. WAG/Rij rats were randomized to four groups (n = 6 each) and underwent subcapsular implantation of 5 x 10(5) CC531 cells into the left liver lobe. The animals of two groups underwent simultaneous PBL of the tumour bearing liver lobe. Ten days later animals underwent a HAI either of temsirolimus (Tem and PBL Tem) or saline solution (Sham and PBL Sham). Tumour size was analyzed at days 10 and 13 using three-dimensional ultrasound. In Sham controls tumour volume increased by 43 %. After PBL Sham tumour volume increased by 52 %. In contrast, in animals undergoing HAI of temsirolimus the tumour growth was not only completely inhibited, but tumour volume was found decreased, irrespective of PBL. After HAI of temsirolimus immunohistochemistry revealed an increased cleaved caspase-3 activity, indicating stimulation of apoptotic cell death. In parallel temsirolimus treatment was associated with a significant reduction of PECAM-1 positive cells within the tumour tissue, implying a reduced tumour vascularisation. HAI of temsirolimus is capable of inhibiting the growth of CC531 colorectal rat liver metastases also after PBL."],["dc.identifier.doi","10.1007/s10585-015-9707-4"],["dc.identifier.isi","000352642000002"],["dc.identifier.pmid","25693517"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37505"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1573-7276"],["dc.relation.issn","0262-0898"],["dc.title","Tumour growth of colorectal rat liver metastases is inhibited by hepatic arterial infusion of the mTOR-inhibitor temsirolimus after portal branch ligation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","738"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Investigative Radiology"],["dc.bibliographiccitation.lastpage","744"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Fries, Peter"],["dc.contributor.author","Seidel, Roland"],["dc.contributor.author","Mueller, Andreas"],["dc.contributor.author","Matthes, Kathrin"],["dc.contributor.author","Denda, Gero"],["dc.contributor.author","Massmann, Alexander"],["dc.contributor.author","Menger, Michael D."],["dc.contributor.author","Sperling, Jens"],["dc.contributor.author","Morelli, John N."],["dc.contributor.author","Altmeyer, Katrin"],["dc.contributor.author","Schneider, Guenther"],["dc.contributor.author","Buecker, Arno"],["dc.date.accessioned","2018-11-07T09:18:46Z"],["dc.date.available","2018-11-07T09:18:46Z"],["dc.date.issued","2013"],["dc.description.abstract","Objective: The aim of this study was to compare a retrospectively self-gated fast low angle shot sequence (RSG-FLASH) with a prospectively triggered fast low angle shot sequence (PT-FLASH) using an external trigger device for dynamic contrast-enhanced magnetic resonance imaging of the liver at 9.4 T in a rat model of colorectal cancer metastases. Materials and Methods: In 10 rats with hepatic metastases, we acquired an axial RSG-FLASH sequence through the liver. A FLASH sequence with prospective triggering (PT-FLASH) using an external trigger device was acquired at the same location with the same imaging parameters. After intravenous injection of 0.2 mmol/kg body weight of Gd-DTPA, alternating acquisitions of both sequences were performed at 4 consecutive time points. Signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and lesion enhancement were obtained for liver tumors and parenchyma. In addition, we assessed the total acquisition times of the different imaging approaches for each acquisition, including triggering and gating. Two independent readers performed a qualitative evaluation of each sequence. Statistical analyses included paired t tests and Wilcoxon matched pairs signed rank tests. Results: No statistically significant differences in SNR, CNR, or lesion enhancement were observed. Qualitative assessments of the sequences were comparable. However, acquisition times of PT-FLASH were significantly longer (mean [SD], 160.6 [25.7] seconds; P < 0.0001) and markedly variable (minimum, 120 seconds; maximum, 209 seconds), whereas the RSG-FLASH approach demonstrated a constant mean (SD) acquisition time of 59.0 (0) seconds. Conclusions: The RSG-FLASH and PT-FLASH sequences do not differ qualitatively or quantitatively regarding SNR, CNR, and lesion enhancement for magnetic resonance imaging of the liver in the rats at 9.4 T. However, the variability of acquisition times for the PT-FLASH sequences is a major factor of inconsistency, and we therefore consider this approach as inappropriate for dynamic contrast-enhanced studies with multiple-measurement time points. In contrast, the RSG-FLASH sequence represents a fast, consistent, and reproducible technique suitable for contrast-agent kinetic studies in experimental small-animal imaging of the abdomen."],["dc.description.sponsorship","BMBF (German Ministry of Education and Research) [0314101]"],["dc.identifier.isi","000330447000008"],["dc.identifier.pmid","23695083"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28479"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1536-0210"],["dc.relation.issn","0020-9996"],["dc.title","Comparison of Self-Gated and Prospectively Triggered Fast Low Angle Shot (FLASH) Sequences for Contrast-Enhanced Magnetic Resonance Imaging of the Liver at 9.4 T in a Rat Model of Colorectal Cancer Metastases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]