Bodenschatz, Eberhard

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PMC Biophysics"],["dc.bibliographiccitation.lastpage","15"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Westendorf, Christian"],["dc.contributor.author","Bae, Albert J."],["dc.contributor.author","Erlenkamper, Christoph"],["dc.contributor.author","Galland, Edouard"],["dc.contributor.author","Franck, Carl"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Beta, Carsten"],["dc.date.accessioned","2019-07-09T11:52:49Z"],["dc.date.available","2019-07-09T11:52:49Z"],["dc.date.issued","2010"],["dc.description.abstract","Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method. PACS Codes: 87.64.-t, 47.61.-k, 87.80.Ek"],["dc.identifier.doi","10.1186/1757-5036-3-9"],["dc.identifier.pmid","20403171"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60285"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.ddc","530"],["dc.title","Live cell flattening -traditional and novel approaches"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e106574"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Bae, Albert J."],["dc.contributor.author","Fuller, Danny"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Rappel, Wouter-Jan"],["dc.contributor.author","Loomis, William F."],["dc.date.accessioned","2018-11-07T09:35:03Z"],["dc.date.available","2018-11-07T09:35:03Z"],["dc.date.issued","2014"],["dc.description.abstract","Vegetative and developed amoebae of Dictyostelium discoideum gain traction and move rapidly on a wide range of substrata without forming focal adhesions. We used two independent assays to quantify cell-substrate adhesion in mutants and in wild-type cells as a function of development. Using a microfluidic device that generates a range of hydrodynamic shear stress, we found that substratum adhesion decreases at least 10 fold during the first 6 hr of development of wild type cells. This result was confirmed using a single-cell assay in which cells were attached to the cantilever of an atomic force probe and allowed to adhere to untreated glass surfaces before being retracted. Both of these assays showed that the decrease in substratum adhesion was dependent on the cAMP receptor CAR1 which triggers development. Vegetative cells missing talin as the result of a mutation in talA exhibited slightly reduced adhesive properties compared to vegetative wildtype cells. In sharp contrast to wild-type cells, however, these talA mutant cells did not show further reduction of adhesion during development such that after 5 hr of development they were significantly more adhesive than developed wild type cells. In addition, both assays showed that substrate adhesion was reduced in 0 hr cells when the actin cytoskeleton was disrupted by latrunculin. Consistent with previous observations, substrate adhesion was also reduced in 0 hr cells lacking the membrane proteins SadA or SibA as the result of mutations in sadA or sibA. However, there was no difference in the adhesion properties between wild type AX3 cells and these mutant cells after 6 hr of development, suggesting that neither SibA nor SadA play an essential role in substratum adhesion during aggregation. Our results provide a quantitative framework for further studies of cell substratum adhesion in Dictyostelium."],["dc.identifier.doi","10.1371/journal.pone.0106574"],["dc.identifier.fs","606792"],["dc.identifier.isi","000342351800013"],["dc.identifier.pmid","25247557"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11605"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32310"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.title","Cell Substratum Adhesion during Early Development of Dictyostelium discoideum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e37213"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Amselem, Gabriel"],["dc.contributor.author","Theves, Matthias"],["dc.contributor.author","Bae, Albert J."],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Beta, Carsten"],["dc.date.accessioned","2018-11-07T09:10:15Z"],["dc.date.available","2018-11-07T09:10:15Z"],["dc.date.issued","2012"],["dc.description.abstract","Chemotaxis, the directed motion of a cell toward a chemical source, plays a key role in many essential biological processes. Here, we derive a statistical model that quantitatively describes the chemotactic motion of eukaryotic cells in a chemical gradient. Our model is based on observations of the chemotactic motion of the social ameba Dictyostelium discoideum, a model organism for eukaryotic chemotaxis. A large number of cell trajectories in stationary, linear chemoattractant gradients is measured, using microfluidic tools in combination with automated cell tracking. We describe the directional motion as the interplay between deterministic and stochastic contributions based on a Langevin equation. The functional form of this equation is directly extracted from experimental data by angle-resolved conditional averages. It contains quadratic deterministic damping and multiplicative noise. In the presence of an external gradient, the deterministic part shows a clear angular dependence that takes the form of a force pointing in gradient direction. With increasing gradient steepness, this force passes through a maximum that coincides with maxima in both speed and directionality of the cells. The stochastic part, on the other hand, does not depend on the orientation of the directional cue and remains independent of the gradient magnitude. Numerical simulations of our probabilistic model yield quantitative agreement with the experimental distribution functions. Thus our model captures well the dynamics of chemotactic cells and can serve to quantify differences and similarities of different chemotactic eukaryotes. Finally, on the basis of our model, we can characterize the heterogeneity within a population of chemotactic cells."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [BE 3978/3-1]"],["dc.identifier.doi","10.1371/journal.pone.0037213"],["dc.identifier.isi","000305342300029"],["dc.identifier.pmid","22662138"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7898"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26447"],["dc.notes","CB acknowledges financial support by the Deutsche Forschungsgemeinschaft (DFG BE 3978/3-1), www.dfg.de."],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Fakultät für Physik"],["dc.title","A Stochastic Description of Dictyostelium Chemotaxis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]