Schild, Detlev

[["dc.bibliographiccitation.artnumber","014022"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Weigel, Arwed"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Zeug, Andre"],["dc.date.accessioned","2018-11-07T08:34:48Z"],["dc.date.available","2018-11-07T08:34:48Z"],["dc.date.issued","2009"],["dc.description.abstract","The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens. Here we compare the resolution of the ApoTome (commercial SIM by Zeiss) to that achieved by a cLSM (Zeiss LSM 510). If fluorescent beads are used as test objects, then the ApoTome will achieve a lower axial resolution than the cLSM. In contrast to that, its lateral resolution scores slightly better. If subresolution homogeneous fluorescent layers are used as test objects, then the ApoTome will achieve a higher axial resolution than the cLSM. The ApoTome's axial resolution is homogeneous over the field-of-view while that of the cLSM changes markedly. Finally, the anisotropy of the ApoTome's resolution was found to be negligible for standard applications while its capability to resolve fine structures within stained tissue slices is limited to one or two cell layers and thus worse than in the cLSM. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3083439]"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.1117/1.3083439"],["dc.identifier.isi","000264551900027"],["dc.identifier.pmid","19256710"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7767"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17904"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Spie-soc Photoptical Instrumentation Engineers"],["dc.relation.issn","1083-3668"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Resolution in the ApoTome and the confocal laser scanning microscope: comparison"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","206"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Neural Regeneration Research"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Chatterjee, Madhurima"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Teunissen, CharlotteE"],["dc.date.accessioned","2020-12-10T18:47:43Z"],["dc.date.available","2020-12-10T18:47:43Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.4103/1673-5374.244776"],["dc.identifier.issn","1673-5374"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78864"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Contactins in the central nervous system: role in health and disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","140"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Neuroscience Methods"],["dc.bibliographiccitation.lastpage","147"],["dc.bibliographiccitation.volume","167"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Schweer, Tina-Saskia"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T11:19:01Z"],["dc.date.available","2018-11-07T11:19:01Z"],["dc.date.issued","2008"],["dc.description.abstract","ATP-binding cassette (ABC) transporters are a family of transmembrane proteins that, also known as multidrug resistance proteins, transport a wide variety of substrates across biological membranes in an energy-dependent manner. Recently it has been shown that members of this protein family interfere with fluorescent (calcium indicator) dye uptake in taste buds of rat and in cells in the olfactory epithelium of larval Xenopus laevis, including olfactory receptor neurons. It has, however, not been resolved whether this effect only serves to extrude xenobiotics in sensory taste and olfactory cells, or alternatively, whether it is a more general feature of many central nervous system neurons. In the latter case blocking these transporters would improve fluorescent dye uptake in general. Here we show, by means of cell imaging. that also neurons of the olfactory bulb express multidrug resistance transporters, whereby a marked inhomogeneity among cells in the main and accessory olfactory bulb was observed. Blocking these transporters improved the net uptake of fluorescent dyes not only in cell somata of the olfactory bulb, but especially in fine neuronal structures such as individual dendrites or olfactory glomeruli, which consist of a tangle of tiny neuronal processes. We therefore suggest that the expression of multidrug resistance proteins may be common in cells of the central nervous system, and that the application of specific transport inhibitors could generally improve fluorescent dye uptake in brain slices, thereby improving calcium imaging conditions. (c) 2007 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.jneumeth.2007.07.018"],["dc.identifier.isi","000252938400002"],["dc.identifier.pmid","17767961"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9767"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55173"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation.issn","0165-0270"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Improved fluorescent (calcium indicator) dye uptake in brain slices by blocking multidrug resistance transporters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1061"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Clinical Biochemistry"],["dc.bibliographiccitation.lastpage","1066"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Chatterjee, Madhurima"],["dc.contributor.author","Nöding, Bernd"],["dc.contributor.author","Willemse, Eline A.J."],["dc.contributor.author","Koel-Simmelink, Marleen J.A."],["dc.contributor.author","van der Flier, Wiesje M."],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Teunissen, Charlotte E."],["dc.date.accessioned","2020-12-10T14:23:07Z"],["dc.date.available","2020-12-10T14:23:07Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.clinbiochem.2017.08.017"],["dc.identifier.issn","0009-9120"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71839"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Detection of contactin-2 in cerebrospinal fluid (CSF) of patients with Alzheimer's disease using Fluorescence Correlation Spectroscopy (FCS)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10978"],["dc.bibliographiccitation.issue","43"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","10989"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Brinkmann, Alexander"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2020-12-10T18:42:36Z"],["dc.date.available","2020-12-10T18:42:36Z"],["dc.date.issued","2016"],["dc.description.abstract","The olfactory system senses odors, but not exclusively, as shown over the past years. It also registers other modalities such as temperature and pressure. However, it remains unknown how widespread these sensitivities are across species and how strongly their processing is interconnected with the processing of odors. Here, we present data on the beta-glomerulus in the olfactory bulb of Xenopus laevis tadpoles. We show that this glomerulus possesses an unusually broad response pattern to a large number of amino acids. The beta-glomerulus uses the classical cAMP-mediated pathway, as suggested by its sensitivity to forskolin. This finding was unexpected because amino acidsensitive olfactory sensory neurons of Xenopus commonly function in a cAMP-independent manner. Furthermore, we show that the beta-glomerulus also reacts to pressure pulses delivered to the olfactory mucosa. These mechanical stimuli induce responses with profiles having typical dose-response and adaptation curves. Finally, whereas the mechanosensitivity in the glomerular layer was observed repeatedly in the beta-glomerulus only, mechanosensitive modulation of mitral cells and their postsynaptic neuropils was found on a larger scale. Some mitral cells closely followed the response time course of the beta-glomerulus, whereas many others were strongly inhibited by short pressure pulses. In conclusion, our data demonstrate the existence of one glomerulus sensitive to both a large number of amino acids and pressure pulses and show that the processing of pressure pulses is intertwined with odor processing."],["dc.identifier.doi","10.1523/JNEUROSCI.4631-15.2016"],["dc.identifier.eissn","1529-2401"],["dc.identifier.isi","000391045800006"],["dc.identifier.issn","0270-6474"],["dc.identifier.pmid","27798179"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78021"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.title","One Special Glomerulus in the Olfactory Bulb of Xenopus laevis Tadpoles Integrates a Broad Range of Amino Acids and Mechanical Stimuli"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1452"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","FEBS Letters"],["dc.bibliographiccitation.lastpage","1458"],["dc.bibliographiccitation.volume","586"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Engelke, Michael"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Wienands, Juergen"],["dc.date.accessioned","2018-11-07T09:10:18Z"],["dc.date.available","2018-11-07T09:10:18Z"],["dc.date.issued","2012"],["dc.description.abstract","Antigen-induced B cell activation requires mobilization of the Ca2+ second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultaneously visualize intracellular Ca2+ flux profiles and the translocation of cytosolic signaling proteins to the plasma membrane in real time. Our approach delineated the kinetic hierarchy of Ca2+ signaling events in B cells and revealed a timely ordered contribution of various organelles to the overall Ca2+ signal. The developed experimental setup provides a useful tool to resolve the spatiotemporal signaling dynamics in various receptor signaling systems. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.febslet.2012.03.057"],["dc.identifier.isi","000304104200011"],["dc.identifier.pmid","22673510"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26456"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0014-5793"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Spatiotemporal resolution of Ca2+ signaling events by real time imaging of single B cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1614"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Glia"],["dc.bibliographiccitation.lastpage","1624"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Hassenklover, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T11:09:09Z"],["dc.date.available","2018-11-07T11:09:09Z"],["dc.date.issued","2008"],["dc.description.abstract","Extracellular purines and pyrimidines are important Signaling molecules acting via purinergic cell-surface receptors in neurons, glia, and glia-like cells such as sustentacular supporting cells (SCs) of the olfactory epithelium (OE). Here, we thoroughly characterize ATP-induced responses in SCs of the OE using functional Ca(2+) image The initial ATP-induced increase of the intracellular Ca(2+) concentration [Ca(2+)](i) always occurred in the apical part of SCs and subsequently propagated toward the basal lamina, indicating the occurrence of purinergic receptors I the apical part of SCs. The mean propagation velocity of the Ca(2+) signal within SCs was 17.10 +/- 1.02 mu m/s. ATP evoked increases in [Ca(2+)](i) in both the presence and absence of extracellular Ca(2+). Depletion of the intracellular Ca(2+) stores abolished the responses. This shows that the ATP-induced [Ca(2+)](i) increases were in large part, if not entirely, due to the activation of G protein-coupled receptors followed by Ca(2+) mobilization from intracellular stores, suggesting an involvement of P2Y receptors. The order of potency of the applied purinergic agonists was UTP > ATP > ATP-gamma S (with all others being only weakly active or inactive). The ATP-induced [Ca(2+)](i) increases could be reduced by the purinergic antagonists PPADS and RB2, but not by suramin. Our findings suggest that extracellular nucleotides in the OE activate SCs via P2Y(2)/P2Y(4)-like receptors and initiate a characteristic intraepithelial Ca(2+) wave. (C) 2008 Wiley-Liss, Inc."],["dc.description.sponsorship","DFG Research Center"],["dc.identifier.doi","10.1002/glia.20714"],["dc.identifier.isi","000260918100002"],["dc.identifier.pmid","18551628"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7757"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52943"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0894-1491"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Nucleotide-Induced Ca(2+) Signaling in Sustentacular Supporting Cells of the Olfactory Epithelium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","61"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Physiological Reviews"],["dc.bibliographiccitation.lastpage","154"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Di Natale, Corrado"],["dc.date.accessioned","2022-01-11T14:06:06Z"],["dc.date.available","2022-01-11T14:06:06Z"],["dc.date.issued","2022"],["dc.description.abstract","The biological olfactory system is the sensory system responsible for the detection of the chemical composition of the environment. Several attempts to mimic biological olfactory systems have led to various artificial olfactory systems using different technical approaches. Here we provide a parallel description of biological olfactory systems and their technical counterparts. We start with a presentation of the input to the systems, the stimuli, and treat the interface between the external world and the environment where receptor neurons or artificial chemosensors reside. We then delineate the functions of receptor neurons and chemosensors as well as their overall input-output (I/O) relationships. Up to this point, our accounts of the systems go along similar lines. The next processing steps differ considerably: whereas in biology the processing step following the receptor neurons is the “integration” and “processing” of receptor neuron outputs in the olfactory bulb, this step has various realizations in electronic noses. For a long period of time, the signal processing stages beyond the olfactory bulb, i.e., the higher olfactory centers, were little studied. Only recently has there been a marked growth of studies tackling the information processing in these centers. In electronic noses, a third stage of processing has virtually never been considered. In this review, we provide an up-to-date overview of the current knowledge of both fields and, for the first time, attempt to tie them together. We hope it will be a breeding ground for better information, communication, and data exchange between very related but so far little-connected fields."],["dc.description.abstract","The biological olfactory system is the sensory system responsible for the detection of the chemical composition of the environment. Several attempts to mimic biological olfactory systems have led to various artificial olfactory systems using different technical approaches. Here we provide a parallel description of biological olfactory systems and their technical counterparts. We start with a presentation of the input to the systems, the stimuli, and treat the interface between the external world and the environment where receptor neurons or artificial chemosensors reside. We then delineate the functions of receptor neurons and chemosensors as well as their overall input-output (I/O) relationships. Up to this point, our accounts of the systems go along similar lines. The next processing steps differ considerably: whereas in biology the processing step following the receptor neurons is the “integration” and “processing” of receptor neuron outputs in the olfactory bulb, this step has various realizations in electronic noses. For a long period of time, the signal processing stages beyond the olfactory bulb, i.e., the higher olfactory centers, were little studied. Only recently has there been a marked growth of studies tackling the information processing in these centers. In electronic noses, a third stage of processing has virtually never been considered. In this review, we provide an up-to-date overview of the current knowledge of both fields and, for the first time, attempt to tie them together. We hope it will be a breeding ground for better information, communication, and data exchange between very related but so far little-connected fields."],["dc.identifier.doi","10.1152/physrev.00036.2020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97825"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation.eissn","1522-1210"],["dc.relation.issn","0031-9333"],["dc.title","Principles of odor coding in vertebrates and artificial chemosensory systems"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2401"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","2406"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Chen, Tsai-Wen"],["dc.contributor.author","Lin, Bei-Jung"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T08:32:42Z"],["dc.date.available","2018-11-07T08:32:42Z"],["dc.date.issued","2009"],["dc.description.abstract","Odor representation in the olfactory bulb (OB) undergoes a transformation from a combinatorial glomerular map to a distributed mitral/tufted (M/T) cell code. To understand this transformation, we analyzed the odor representation in large populations of individual M/T cells in the Xenopus OB. The spontaneous [Ca(2+)] activities of M/T cells appeared to be irregular, but there were groups of spatially distributed neurons showing synchronized [Ca(2+)] activities. These neurons were always connected to the same glomerulus. Odorants elicited complex spatiotemporal response patterns in M/T cells where nearby neurons generally showed little correlation. But the responses of neurons connected to the same glomerulus were virtually identical, irrespective of whether the responses were excitatory or inhibitory, and independent of the distance between them. Synchronous neurons received correlated EPSCs and were coupled by electrical conductances that could account for the correlated responses. Thus, at the output stage of the OB, odors are represented by modules of distributed and synchronous M/T cells associated with the same glomeruli. This allows for parallel input to higher brain centers."],["dc.identifier.doi","10.1073/pnas.0810151106"],["dc.identifier.isi","000263516100058"],["dc.identifier.pmid","19181842"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6317"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17398"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Odor coding by modules of coherent mitral/tufted cells in the vertebrate olfactory bulb"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2985"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","2995"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","de Jong, Danielle"],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T09:48:00Z"],["dc.date.available","2018-11-07T09:48:00Z"],["dc.date.issued","2015"],["dc.description.abstract","Olfactory receptor neurons (ORNs) have high-voltage-gated Ca2+ channels whose physiological impact has remained enigmatic since the voltage-gated conductances in this cell type were first described in the 1980s. Here we show that in ORN somata of Xenopus laevis tadpoles these channels are clustered and co-expressed with large-conductance potassium (BK) channels. We found approximately five clusters per ORN and twelve Ca2+ channels per cluster. The action potential-triggered activation of BK channels accelerates the repolarization of action potentials and shortens interspike intervals during odour responses. This increases the sensitivity of individual ORNs to odorants. At the level of mitral cells of the olfactory bulb, odour qualities have been shown to be coded by first-spike-latency patterns. The system of Ca2+ and BK channels in ORNs appears to be important for correct odour coding because the blockage of BK channels not only affects ORN spiking patterns but also changes the latency pattern representation of odours in the olfactory bulb."],["dc.identifier.doi","10.1111/ejn.13095"],["dc.identifier.isi","000368243100011"],["dc.identifier.pmid","26452167"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35218"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1460-9568"],["dc.relation.issn","0953-816X"],["dc.title","Ca2+-BK channel clusters in olfactory receptor neurons and their role in odour coding"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]