Schild, Detlev

[["dc.bibliographiccitation.firstpage","1452"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","FEBS Letters"],["dc.bibliographiccitation.lastpage","1458"],["dc.bibliographiccitation.volume","586"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Engelke, Michael"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Wienands, Juergen"],["dc.date.accessioned","2018-11-07T09:10:18Z"],["dc.date.available","2018-11-07T09:10:18Z"],["dc.date.issued","2012"],["dc.description.abstract","Antigen-induced B cell activation requires mobilization of the Ca2+ second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultaneously visualize intracellular Ca2+ flux profiles and the translocation of cytosolic signaling proteins to the plasma membrane in real time. Our approach delineated the kinetic hierarchy of Ca2+ signaling events in B cells and revealed a timely ordered contribution of various organelles to the overall Ca2+ signal. The developed experimental setup provides a useful tool to resolve the spatiotemporal signaling dynamics in various receptor signaling systems. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.febslet.2012.03.057"],["dc.identifier.isi","000304104200011"],["dc.identifier.pmid","22673510"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26456"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0014-5793"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Spatiotemporal resolution of Ca2+ signaling events by real time imaging of single B cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1614"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Glia"],["dc.bibliographiccitation.lastpage","1624"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Hassenklover, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T11:09:09Z"],["dc.date.available","2018-11-07T11:09:09Z"],["dc.date.issued","2008"],["dc.description.abstract","Extracellular purines and pyrimidines are important Signaling molecules acting via purinergic cell-surface receptors in neurons, glia, and glia-like cells such as sustentacular supporting cells (SCs) of the olfactory epithelium (OE). Here, we thoroughly characterize ATP-induced responses in SCs of the OE using functional Ca(2+) image The initial ATP-induced increase of the intracellular Ca(2+) concentration [Ca(2+)](i) always occurred in the apical part of SCs and subsequently propagated toward the basal lamina, indicating the occurrence of purinergic receptors I the apical part of SCs. The mean propagation velocity of the Ca(2+) signal within SCs was 17.10 +/- 1.02 mu m/s. ATP evoked increases in [Ca(2+)](i) in both the presence and absence of extracellular Ca(2+). Depletion of the intracellular Ca(2+) stores abolished the responses. This shows that the ATP-induced [Ca(2+)](i) increases were in large part, if not entirely, due to the activation of G protein-coupled receptors followed by Ca(2+) mobilization from intracellular stores, suggesting an involvement of P2Y receptors. The order of potency of the applied purinergic agonists was UTP > ATP > ATP-gamma S (with all others being only weakly active or inactive). The ATP-induced [Ca(2+)](i) increases could be reduced by the purinergic antagonists PPADS and RB2, but not by suramin. Our findings suggest that extracellular nucleotides in the OE activate SCs via P2Y(2)/P2Y(4)-like receptors and initiate a characteristic intraepithelial Ca(2+) wave. (C) 2008 Wiley-Liss, Inc."],["dc.description.sponsorship","DFG Research Center"],["dc.identifier.doi","10.1002/glia.20714"],["dc.identifier.isi","000260918100002"],["dc.identifier.pmid","18551628"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7757"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52943"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0894-1491"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Nucleotide-Induced Ca(2+) Signaling in Sustentacular Supporting Cells of the Olfactory Epithelium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2611"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2619"],["dc.bibliographiccitation.volume","101"],["dc.contributor.author","Agte, Silke"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Matthias, Sabrina"],["dc.contributor.author","Ulbricht, Elke"],["dc.contributor.author","Erdmann, Ines"],["dc.contributor.author","Wurm, Antje"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Kaes, Josef A."],["dc.contributor.author","Reichenbach, Andreas"],["dc.date.accessioned","2018-11-07T08:48:58Z"],["dc.date.available","2018-11-07T08:48:58Z"],["dc.date.issued","2011"],["dc.description.abstract","In vertebrate eyes, images are projected onto an inverted retina where light passes all retinal layers on its way to the photoreceptor cells. Light scattering within this tissue should impair vision. We show that radial glial (Muller) cells in the living retina minimize intraretinal light scatter and conserve the diameter of a beam that hits a single Muller cell endfoot. Thus, light arrives at individual photoreceptors with high intensity. This leads to an optimized signal/noise ratio, which increases visual sensitivity and contrast. Moreover, we show that the ratio between Muller cells and cones-responsible for acute vision-is roughly 1. This suggests that high spatiotemporal resolution may be achieved by each cone receiving its part of the image via its individual Muller cell-light guide."],["dc.identifier.doi","10.1016/j.bpj.2011.09.062"],["dc.identifier.isi","000297897300010"],["dc.identifier.pmid","22261048"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7621"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21342"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","0006-3495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Muller Glial Cell-Provided Cellular Light Guidance through the Vital Guinea-Pig Retina"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","E3"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.lastpage","E4"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Wolf, Fred"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T09:00:59Z"],["dc.date.available","2018-11-07T09:00:59Z"],["dc.date.issued","2011"],["dc.identifier.isi","000285414900012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24299"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.eventlocation","Avignon, FRANCE"],["dc.relation.issn","0379-864X"],["dc.title","Fast simultaneous imaging of mitral cell populations reveals odor specificity of latency patterns"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","E61"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.lastpage","E62"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Hassenkloever, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T08:31:54Z"],["dc.date.available","2018-11-07T08:31:54Z"],["dc.date.issued","2009"],["dc.identifier.isi","000263408400198"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17222"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","18th Congress of the European-Chemoreception-Research-Organization"],["dc.relation.eventlocation","Univ Lyubljana, Bernardin, SLOVENIA"],["dc.relation.issn","0379-864X"],["dc.title","Purinergic Signaling in the Olfactory Epithelium"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","A6"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.lastpage","A7"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Hassenkloever, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T11:24:36Z"],["dc.date.available","2018-11-07T11:24:36Z"],["dc.date.issued","2009"],["dc.identifier.isi","000269196800022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56443"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","31st Annual Meeting of the Association-for-Chemoreception-Sciences"],["dc.relation.eventlocation","Sarasota, FL"],["dc.relation.issn","0379-864X"],["dc.title","Nucleotide-Mediated Signaling in the Olfactory Epithelium"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Chen, Tsai-Wen"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T09:00:58Z"],["dc.date.available","2018-11-07T09:00:58Z"],["dc.date.issued","2011"],["dc.format.extent","E56"],["dc.identifier.isi","000285414900151"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24296"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","20th Congress of European Chemoreception Research Organization (ECRO-2010)"],["dc.relation.eventlocation","Avignon, FRANCE"],["dc.relation.issn","0379-864X"],["dc.title","Activity correlation imaging: visualizing function and structure of neuronal populations in the olfactory bulb"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","872"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","884"],["dc.bibliographiccitation.volume","67"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Wolf, Fred"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2017-09-07T11:45:41Z"],["dc.date.available","2017-09-07T11:45:41Z"],["dc.date.issued","2010"],["dc.description.abstract","The encoding of odors by spatiotemporal patterns of mitral/tufted (M/T) cells in the vertebrate olfactory bulb has been discussed controversially. Motivated by temporal constraints from behavioral studies, we investigated the information contained in odor-evoked first-spike latencies. Using simultaneous recordings of dozens of M/T cells with a high temporal resolution and quantitative ensemble correlation techniques, we show that latency patterns, and in particular latency rank patterns, are highly odor specific and reproducible. They reliably predict the odor identity as well as the odor concentration on a single-trial basis and on short timescales—in fact, more reliably than patterns of firing rates. Furthermore, we show that latency ranks exhibit a better reproducibility at the level of M/T cells than in olfactory receptor neurons. Our results suggest that the latency patterns of M/T cells contain all the information higher brain centers need to identify odors and their concentrations."],["dc.identifier.doi","10.1016/j.neuron.2010.08.005"],["dc.identifier.gro","3151845"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6314"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8672"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","0896-6273"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Olfactory Coding with Patterns of Response Latencies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]