Schild, Detlev

[["dc.bibliographiccitation.firstpage","140"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Neuroscience Methods"],["dc.bibliographiccitation.lastpage","147"],["dc.bibliographiccitation.volume","167"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Schweer, Tina-Saskia"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T11:19:01Z"],["dc.date.available","2018-11-07T11:19:01Z"],["dc.date.issued","2008"],["dc.description.abstract","ATP-binding cassette (ABC) transporters are a family of transmembrane proteins that, also known as multidrug resistance proteins, transport a wide variety of substrates across biological membranes in an energy-dependent manner. Recently it has been shown that members of this protein family interfere with fluorescent (calcium indicator) dye uptake in taste buds of rat and in cells in the olfactory epithelium of larval Xenopus laevis, including olfactory receptor neurons. It has, however, not been resolved whether this effect only serves to extrude xenobiotics in sensory taste and olfactory cells, or alternatively, whether it is a more general feature of many central nervous system neurons. In the latter case blocking these transporters would improve fluorescent dye uptake in general. Here we show, by means of cell imaging. that also neurons of the olfactory bulb express multidrug resistance transporters, whereby a marked inhomogeneity among cells in the main and accessory olfactory bulb was observed. Blocking these transporters improved the net uptake of fluorescent dyes not only in cell somata of the olfactory bulb, but especially in fine neuronal structures such as individual dendrites or olfactory glomeruli, which consist of a tangle of tiny neuronal processes. We therefore suggest that the expression of multidrug resistance proteins may be common in cells of the central nervous system, and that the application of specific transport inhibitors could generally improve fluorescent dye uptake in brain slices, thereby improving calcium imaging conditions. (c) 2007 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.jneumeth.2007.07.018"],["dc.identifier.isi","000252938400002"],["dc.identifier.pmid","17767961"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9767"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55173"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation.issn","0165-0270"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Improved fluorescent (calcium indicator) dye uptake in brain slices by blocking multidrug resistance transporters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1614"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Glia"],["dc.bibliographiccitation.lastpage","1624"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Hassenklover, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T11:09:09Z"],["dc.date.available","2018-11-07T11:09:09Z"],["dc.date.issued","2008"],["dc.description.abstract","Extracellular purines and pyrimidines are important Signaling molecules acting via purinergic cell-surface receptors in neurons, glia, and glia-like cells such as sustentacular supporting cells (SCs) of the olfactory epithelium (OE). Here, we thoroughly characterize ATP-induced responses in SCs of the OE using functional Ca(2+) image The initial ATP-induced increase of the intracellular Ca(2+) concentration [Ca(2+)](i) always occurred in the apical part of SCs and subsequently propagated toward the basal lamina, indicating the occurrence of purinergic receptors I the apical part of SCs. The mean propagation velocity of the Ca(2+) signal within SCs was 17.10 +/- 1.02 mu m/s. ATP evoked increases in [Ca(2+)](i) in both the presence and absence of extracellular Ca(2+). Depletion of the intracellular Ca(2+) stores abolished the responses. This shows that the ATP-induced [Ca(2+)](i) increases were in large part, if not entirely, due to the activation of G protein-coupled receptors followed by Ca(2+) mobilization from intracellular stores, suggesting an involvement of P2Y receptors. The order of potency of the applied purinergic agonists was UTP > ATP > ATP-gamma S (with all others being only weakly active or inactive). The ATP-induced [Ca(2+)](i) increases could be reduced by the purinergic antagonists PPADS and RB2, but not by suramin. Our findings suggest that extracellular nucleotides in the OE activate SCs via P2Y(2)/P2Y(4)-like receptors and initiate a characteristic intraepithelial Ca(2+) wave. (C) 2008 Wiley-Liss, Inc."],["dc.description.sponsorship","DFG Research Center"],["dc.identifier.doi","10.1002/glia.20714"],["dc.identifier.isi","000260918100002"],["dc.identifier.pmid","18551628"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7757"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52943"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0894-1491"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Nucleotide-Induced Ca(2+) Signaling in Sustentacular Supporting Cells of the Olfactory Epithelium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","61"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Physiological Reviews"],["dc.bibliographiccitation.lastpage","154"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Di Natale, Corrado"],["dc.date.accessioned","2022-01-11T14:06:06Z"],["dc.date.available","2022-01-11T14:06:06Z"],["dc.date.issued","2022"],["dc.description.abstract","The biological olfactory system is the sensory system responsible for the detection of the chemical composition of the environment. Several attempts to mimic biological olfactory systems have led to various artificial olfactory systems using different technical approaches. Here we provide a parallel description of biological olfactory systems and their technical counterparts. We start with a presentation of the input to the systems, the stimuli, and treat the interface between the external world and the environment where receptor neurons or artificial chemosensors reside. We then delineate the functions of receptor neurons and chemosensors as well as their overall input-output (I/O) relationships. Up to this point, our accounts of the systems go along similar lines. The next processing steps differ considerably: whereas in biology the processing step following the receptor neurons is the “integration” and “processing” of receptor neuron outputs in the olfactory bulb, this step has various realizations in electronic noses. For a long period of time, the signal processing stages beyond the olfactory bulb, i.e., the higher olfactory centers, were little studied. Only recently has there been a marked growth of studies tackling the information processing in these centers. In electronic noses, a third stage of processing has virtually never been considered. In this review, we provide an up-to-date overview of the current knowledge of both fields and, for the first time, attempt to tie them together. We hope it will be a breeding ground for better information, communication, and data exchange between very related but so far little-connected fields."],["dc.description.abstract","The biological olfactory system is the sensory system responsible for the detection of the chemical composition of the environment. Several attempts to mimic biological olfactory systems have led to various artificial olfactory systems using different technical approaches. Here we provide a parallel description of biological olfactory systems and their technical counterparts. We start with a presentation of the input to the systems, the stimuli, and treat the interface between the external world and the environment where receptor neurons or artificial chemosensors reside. We then delineate the functions of receptor neurons and chemosensors as well as their overall input-output (I/O) relationships. Up to this point, our accounts of the systems go along similar lines. The next processing steps differ considerably: whereas in biology the processing step following the receptor neurons is the “integration” and “processing” of receptor neuron outputs in the olfactory bulb, this step has various realizations in electronic noses. For a long period of time, the signal processing stages beyond the olfactory bulb, i.e., the higher olfactory centers, were little studied. Only recently has there been a marked growth of studies tackling the information processing in these centers. In electronic noses, a third stage of processing has virtually never been considered. In this review, we provide an up-to-date overview of the current knowledge of both fields and, for the first time, attempt to tie them together. We hope it will be a breeding ground for better information, communication, and data exchange between very related but so far little-connected fields."],["dc.identifier.doi","10.1152/physrev.00036.2020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97825"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation.eissn","1522-1210"],["dc.relation.issn","0031-9333"],["dc.title","Principles of odor coding in vertebrates and artificial chemosensory systems"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Czesnik, D."],["dc.contributor.author","Kuduz, Josko"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T09:43:57Z"],["dc.date.available","2018-11-07T09:43:57Z"],["dc.date.issued","2006"],["dc.format.extent","A12"],["dc.identifier.isi","000238761600049"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34291"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","28th Annual Meeting of the Association-for-Chemoreception-Sciences"],["dc.relation.eventlocation","Sarasota, FL"],["dc.relation.issn","0379-864X"],["dc.title","Dual effect of ATP in the olfactory epithelium of Xenopus laevis tadpoles: Activation of both receptor and sustentacular supporting cells"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","99"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","The Journal of General Physiology"],["dc.bibliographiccitation.lastpage","107"],["dc.bibliographiccitation.volume","123"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T10:51:27Z"],["dc.date.available","2018-11-07T10:51:27Z"],["dc.date.issued","2004"],["dc.description.abstract","In olfactory receptor neurons (ORNs) of aquatic animals amino acids have been shown to be potent stimuli. Here we report on calcium imaging experiments in slices of the olfactory mucosa of Xenopus laevis tadpoles. We were able to determine the response profiles of 283 ORNs to 19 amino acids, where one profile comprises the responses of one ORN to 19 amino acids. 204 out of the 283 response profiles differed from each other. 36 response spectra occurred more than once, i.e., there were 36 classes of ORNs identically responding to the 19 amino acids. The number of ORNs that formed a class ranged from 2 to 13. Shape and duration of amino acid-elicited [Ca2+](i) transients showed a high degree of similarity upon repeated Stimulation with the same amino acid. Different amino acids, however, in some cases led to clearly distinguishable calcium responses in individual ORNs. Furthermore, ORNs clearly appeared to gain selectivity over time, i.e., ORNs of later developmental stages responded to less amino acids than ORNs of earlier stages. We discuss the narrowing of ORNT selectivity over stages in the context of expression of olfactory receptors."],["dc.identifier.doi","10.1085/jgp.200308970"],["dc.identifier.isi","000188785000002"],["dc.identifier.pmid","14744986"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48896"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Rockefeller Univ Press"],["dc.relation.issn","0022-1295"],["dc.title","Classes and narrowing selectivity of olfactory receptor neurons of Xenopus laevis tadpoles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","925"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","934"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Heermann, Stephan"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Brase, Christoph"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Roessler, Wolfgang"],["dc.date.accessioned","2018-11-07T10:59:55Z"],["dc.date.available","2018-11-07T10:59:55Z"],["dc.date.issued","2007"],["dc.description.abstract","The sensory input layer in the olfactory bulb (OB) is typically organized into spheroidal aggregates of dense neuropil called glomeruli. This characteristic compartmentalization of the synaptic neuropil is a typical feature of primary olfactory centres in vertebrates and most advanced invertebrates. In the present work we mapped the location of presynaptic sites in glomeruli across the OB using antibodies to presynaptic vesicle proteins and presynaptic membrane proteins in combination with confocal microscopy. In addition the responses of glomeruli upon mucosal application of amino acid-odorants and forskolin were monitored using functional calcium imaging. We first describe the spatial distribution of glomeruli across the main olfactory bulb (MOB) in premetamorphic Xenopus laevis. Second, we show that the heterogeneous organization of glomeruli along the dorsoventral and mediolateral axes of the MOB is associated with a differential distribution of synaptic vesicle proteins. While antibodies to synaptophysin, syntaxin and SNAP-25 uniformly labelled glomeruli in the whole MOB, intense synaptotagmin staining was present only in glomeruli in the lateral, and to a lesser extent in the intermediate, part of the OB. Interestingly, amino acid-responsive glomeruli were always located in the lateral part of the OB, and glomeruli activated by mucosal forskolin application were exclusively located in the medial part of the OB. This correlation between odour mapping and presynaptic protein distribution is an additional hint on the existence of different subsystems within the main olfactory system in larval Xenopus laevis."],["dc.identifier.doi","10.1111/j.1460-9568.2007.05731.x"],["dc.identifier.isi","000248963400013"],["dc.identifier.pmid","17666078"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7761"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50808"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0953-816X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Presynaptic protein distribution and odour mapping in glomeruli of the olfactory bulb of Xenopus laevis tadpoles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","475"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","The Journal of Physiology"],["dc.bibliographiccitation.lastpage","484"],["dc.bibliographiccitation.volume","545"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Rossler, W."],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T09:46:19Z"],["dc.date.available","2018-11-07T09:46:19Z"],["dc.date.issued","2002"],["dc.description.abstract","We report on responses of olfactory receptor neurons (ORNs) upon application of amino acids and forskolin using a novel slice preparation of the olfactory epithelium of Xenopus laevis tadpoles. Responses were measured using the patch-damp technique. Both amino acids and forskolin proved to be potent stimuli. Interestingly, a number of ORNs that responded to amino acids did not respond to forskolin. This suggests that some amino acids activate transduction pathways other than the well-known cAMP-mediated one. The differential processing of cAMP-mediated stimuli on the one hand and amino acid stimuli on the other was further elucidated by calcium-imaging of olfactory bulb neurons using a novel nose-olfactory bulb preparation of Xenopus laevis tadpoles. The projection pattern of amino acid-sensitive ORNs to olfactory bulb neurons differed markedly from the projection pattern of forskolin-sensitive ORNs. Olfactory bulb neurons activated by amino acids were located laterally compared to those activated by forskolin, and only a small proportion responded to both stimuli. The ensemble of neurons activated by forskolin was also activated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and the membrane-permeant cAMP analogue 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (pCPT-cAMP). We therefore conclude that sensory transduction of a number of amino acids is cAMP independent, and amino acid- and cAMP-mediated responses are processed differentially at the level of the olfactory bulb."],["dc.identifier.doi","10.1113/jphysiol.2002.031914"],["dc.identifier.isi","000180075200016"],["dc.identifier.pmid","12456827"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34844"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cambridge Univ Press"],["dc.relation.issn","0022-3751"],["dc.title","cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","E61"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.lastpage","E62"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Hassenkloever, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T08:31:54Z"],["dc.date.available","2018-11-07T08:31:54Z"],["dc.date.issued","2009"],["dc.identifier.isi","000263408400198"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17222"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","18th Congress of the European-Chemoreception-Research-Organization"],["dc.relation.eventlocation","Univ Lyubljana, Bernardin, SLOVENIA"],["dc.relation.issn","0379-864X"],["dc.title","Purinergic Signaling in the Olfactory Epithelium"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","A6"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Chemical Senses"],["dc.bibliographiccitation.lastpage","A7"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Hassenkloever, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T11:24:36Z"],["dc.date.available","2018-11-07T11:24:36Z"],["dc.date.issued","2009"],["dc.identifier.isi","000269196800022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56443"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","31st Annual Meeting of the Association-for-Chemoreception-Sciences"],["dc.relation.eventlocation","Sarasota, FL"],["dc.relation.issn","0379-864X"],["dc.title","Nucleotide-Mediated Signaling in the Olfactory Epithelium"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2315"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","2326"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Gliem, Sebastian"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T08:29:08Z"],["dc.date.available","2018-11-07T08:29:08Z"],["dc.date.issued","2009"],["dc.description.abstract","The main olfactory system of larval Xenopus laevis is made up of at least two subsystems consisting of subsets of olfactory receptor neurons (ORNs) with different transduction mechanisms. One ORN subset lacks the canonical cAMP transduction pathway and responds to amino acid odorants. The second subset has the cAMP transduction pathway but as yet suitable odorants are unknown. Here we report the identification of amines as proper olfactory stimuli for larval X. laevis using functional Ca(2+) imaging and slice preparations of the olfactory system. The response profiles of individual ORNs to a number of amines were extremely complex and mostly highly specific. The great majority of amine-sensitive ORNs responded also to forskolin, an activator of the olfactory cAMP transduction pathway. Most amine-induced responses could be attenuated by the cyclic nucleotide-gated channel inhibitor LY83583. This confirms that most amine-responsive olfactory receptors (ORs) are coupled to the cAMP-dependent transduction pathway. Furthermore, we show that trace amine-associated receptors (TAARs), which have been shown to act as specific ORs for amines in mammals, are expressed in the olfactory organ of X. laevis. The TAARs expressed in Xenopus cannot, however, explain the complex responses of individual ORNs to amines because there are too few of them. This indicates that, in addition to TAARs, there must be other receptor families involved in the detection of amines."],["dc.identifier.doi","10.1111/j.1460-9568.2009.06778.x"],["dc.identifier.isi","000267070200006"],["dc.identifier.pmid","19490026"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7756"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16575"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0953-816X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Highly specific responses to amine odorants of individual olfactory receptor neurons in situ"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]